2. GALENVS
  3. PR0050-12

GALENVS PR0050-12 User guide

  • Hello! I've reviewed the Plant RNA Extraction Kit Quick Start Guide and understand the procedures for RNA extraction from plant leaves using this kit. The guide details the steps involving lysis, magnetic bead-based purification with specialized buffers, and DNase treatment. I'm ready to answer your questions about the kit and its usage.
  • How much plant material should I use?
    What speed and time should I use for the centrifuge in step 2?
    How long should the mixture incubate after adding DNase buffer in step 11?
19 Place tube on magnetic rack, wait for 1 min
then transfer supernatant to clean tube.
18
Elution Buer
Add 50µl of Elution Buer to tube and mix
briefly.
17 Dry beads on magnetic rack for 2 mins
after the third wash.
Remove any wash buer left at the bottom
of tube at the end of drying step.
16
Wash Buer #3
Repeat washing step with Wash Buer #3.Resuspend beads in 600µl Wash Buer #3.
Vortex for 10–20s.
Place tube on magnetic rack, wait for 1 min
and discard supernatant.
Wash Buer #3
15
Place tube on magnetic rack for 2 mins.
Discard supernatant.
1413 Add 400µl of Binding Bead and Binding
Buer #2 mixture to microfuge tube and
vortex for 10s.
Incubate the mixture for 5 mins at room
temperature.
Binding Bead &
Buer Mix
12 Mix 40µl of Binding Beads with 400µl
Binding Buer #2.
Binding Buer #2
11 Gently shake the mix for 20s, and incubate
at room temperature for 25 mins.
10
DNase Buer
Add 50µl of DNase buer to 50µl of RNA
sample in a microfuge tube.
9Add 2.5ml of DNase Reaction Buer to
DNase pellet.
Mix by gently inverting the tube.
DNase Reaction Buer
Wait 1 min then transfer supernatant to
clean microfuge tube..
8
Add 100µl of Elution Buer to tube and
mix briefly. Wait 1 min.
Place tube on magnetic rack to capture.
Elution Buer
7
Add 600µl of Wash Buer #2 to the tube
and vortex for 10–20s.
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #2
6
Add 600µl of Wash Buer #1 to the tube
and vortex for 10–20s.
Wait 1 min then place tube on magnetic
rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #1
5
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
4
Avoiding pellet, transfer up to 300–400µl of
supernatant to clean centrifuge tube.
Add 1000µl of Binding Buer #1, vortex for
10–20s, and wait 5 mins.
Binding Buer #1
32 Add 600µl of Lysis Buer and 60µl PPB.
Mix for 3 mins using TissueLyser at max
speed or vortex for 10 mins; then
centrifuge at 20,000g for 2 mins.
20,000g
PPB
Lysis Buer
Add up to 50mg of ground fresh plant
leaves to the lysis bead tube provided.
1
Plant RNA Extraction Kit
 1.0

Plant RNA Extraction Kit
Quick Start Guide
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