GALENVS PR0050-12 User guide

Brand
GALENVS
Model
PR0050-12
Type
User guide

GALENVS PR0050-12

The GALENVS PR0050-12 is a powerful and versatile plant RNA extraction kit that can be used to isolate high-quality RNA from a variety of plant tissues. The kit utilizes a combination of mechanical and chemical lysis to efficiently break down plant cells and release their RNA contents. The RNA is then purified using a series of wash buffers and eluted in a small volume of elution buffer. The eluted RNA can be used for a variety of downstream applications, such as RT-PCR, qPCR, and RNA sequencing.

Here are some of the key features and benefits of the GALENVS PR0050-12 Plant RNA Extraction Kit:

GALENVS PR0050-12

The GALENVS PR0050-12 is a powerful and versatile plant RNA extraction kit that can be used to isolate high-quality RNA from a variety of plant tissues. The kit utilizes a combination of mechanical and chemical lysis to efficiently break down plant cells and release their RNA contents. The RNA is then purified using a series of wash buffers and eluted in a small volume of elution buffer. The eluted RNA can be used for a variety of downstream applications, such as RT-PCR, qPCR, and RNA sequencing.

Here are some of the key features and benefits of the GALENVS PR0050-12 Plant RNA Extraction Kit:

19 Place tube on magnetic rack, wait for 1 min
then transfer supernatant to clean tube.
18
Elution Buer
Add 50µl of Elution Buer to tube and mix
briefly.
17 Dry beads on magnetic rack for 2 mins
after the third wash.
Remove any wash buer left at the bottom
of tube at the end of drying step.
16
Wash Buer #3
Repeat washing step with Wash Buer #3.Resuspend beads in 600µl Wash Buer #3.
Vortex for 10–20s.
Place tube on magnetic rack, wait for 1 min
and discard supernatant.
Wash Buer #3
15
Place tube on magnetic rack for 2 mins.
Discard supernatant.
1413 Add 400µl of Binding Bead and Binding
Buer #2 mixture to microfuge tube and
vortex for 10s.
Incubate the mixture for 5 mins at room
temperature.
Binding Bead &
Buer Mix
12 Mix 40µl of Binding Beads with 400µl
Binding Buer #2.
Binding Buer #2
11 Gently shake the mix for 20s, and incubate
at room temperature for 25 mins.
10
DNase Buer
Add 50µl of DNase buer to 50µl of RNA
sample in a microfuge tube.
9Add 2.5ml of DNase Reaction Buer to
DNase pellet.
Mix by gently inverting the tube.
DNase Reaction Buer
Wait 1 min then transfer supernatant to
clean microfuge tube..
8
Add 100µl of Elution Buer to tube and
mix briefly. Wait 1 min.
Place tube on magnetic rack to capture.
Elution Buer
7
Add 600µl of Wash Buer #2 to the tube
and vortex for 10–20s.
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #2
6
Add 600µl of Wash Buer #1 to the tube
and vortex for 10–20s.
Wait 1 min then place tube on magnetic
rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #1
5
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
4
Avoiding pellet, transfer up to 300–400µl of
supernatant to clean centrifuge tube.
Add 1000µl of Binding Buer #1, vortex for
10–20s, and wait 5 mins.
Binding Buer #1
32 Add 600µl of Lysis Buer and 60µl PPB.
Mix for 3 mins using TissueLyser at max
speed or vortex for 10 mins; then
centrifuge at 20,000g for 2 mins.
20,000g
PPB
Lysis Buer
Add up to 50mg of ground fresh plant
leaves to the lysis bead tube provided.
1
Plant RNA Extraction Kit
 1.0

Plant RNA Extraction Kit
Quick Start Guide
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GALENVS PR0050-12 User guide

Brand
GALENVS
Model
PR0050-12
Type
User guide

GALENVS PR0050-12

The GALENVS PR0050-12 is a powerful and versatile plant RNA extraction kit that can be used to isolate high-quality RNA from a variety of plant tissues. The kit utilizes a combination of mechanical and chemical lysis to efficiently break down plant cells and release their RNA contents. The RNA is then purified using a series of wash buffers and eluted in a small volume of elution buffer. The eluted RNA can be used for a variety of downstream applications, such as RT-PCR, qPCR, and RNA sequencing.

Here are some of the key features and benefits of the GALENVS PR0050-12 Plant RNA Extraction Kit:

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