GALENVS magneti User guide

magneti
Brand
GALENVS
Model
magneti
Type
User guide

GALENVS magneti is a compact, versatile, and easy-to-use nucleic acid purification system ideal for research and diagnostic applications. Its magnetic bead technology enables rapid and efficient extraction of high-quality nucleic acids from various samples, including blood, cells, tissues, plants, and bacteria. With GALENVS magneti, you can expect:

  • Fast and efficient nucleic acid extraction: The system utilizes magnetic beads to quickly capture and purify nucleic acids, reducing processing time and increasing productivity.

  • High-quality nucleic acid yield: GALENVS magneti delivers high-quality nucleic acids suitable for various downstream applications, including PCR, qPCR, sequencing, and genotyping.

GALENVS magneti is a compact, versatile, and easy-to-use nucleic acid purification system ideal for research and diagnostic applications. Its magnetic bead technology enables rapid and efficient extraction of high-quality nucleic acids from various samples, including blood, cells, tissues, plants, and bacteria. With GALENVS magneti, you can expect:

  • Fast and efficient nucleic acid extraction: The system utilizes magnetic beads to quickly capture and purify nucleic acids, reducing processing time and increasing productivity.

  • High-quality nucleic acid yield: GALENVS magneti delivers high-quality nucleic acids suitable for various downstream applications, including PCR, qPCR, sequencing, and genotyping.

12 Dry beads on magnetic rack for 2 mins
after the third wash.
Remove any wash buer left at the
bottom of tube at the end of drying step.
13 Add 90µl of Elution Buer A.
Pipette 20x and incubate for 5 mins.
Place tube on magnetic rack for 5 mins.
14 Transfer supernatant to clean microfuge
tube.
For part only 50µl of the elution is required.
!
Repeat washing step with 600µl Wash
Buer A2.
Place tube on magnetic rack for 2 mins,
then discard supernatant.
11
7 8 9
Add 40µl of Proteinase K. Add 300µl of Binding Buer A and mix by
pipetting.
Vortex tube for 15 minutes with a vortex
shaker at moderate speed.
5
5000g
Suspension
Buer
Proteinase K Binding Buer A
Resuspend pellet in 1.85ml nuclease-free,
sterile water.
Transfer suspension to microfuge tube.
Spin down tube again at 5000g for 10
mins.
Discard supernatant.
Resuspend pellet in 300µl of Suspension
Buer.
Resuspend beads in 600µl Wash Buer A1.
Mix by vortex shaker for 5 mins at
moderate speed.
Place tube on magnetic rack for 2 mins to
capture beads, then discard supernatant.
2 3 4
Nuclease-free
water
Thaw Paxgene tube for 2 hours at room
temperature.
Spin down PAXgene® tube at 5000g for
10 mins. Discard supernatant.
1
Add 40µl of Binding Beads A and mix by
pipetting.
Vortex tube for 10 minutes with a vortex
shaker.
Place tube on magnetic rack for 2 mins to
capture the RNA-bead complex.
Discard supernatant.
6
Resuspend beads in 600µl Wash Buer A2.
Mix by pipetting 20x.
Place tube on magnetic rack for 2 mins,
then discard supernatant.
10
5000g
Binding Beads A Wash Buer A1
Wash Buer A2 Wash Buer A2
Elution Buer A
Proceed to part DNase Treatment
Total RNA Extraction Kit
Quick Start Guide
Part RNA Extraction
22 Place tube on magnetic rack for 2 mins.
Discard supernatant.
Resuspend beads in 600µl Wash Buer B1.
Mix by pipetting 20x.
23
Wash Buer B1
DNase
Wash Buer B1
Elution Buer B
Dry beads on magnetic rack for 2 mins
after the second wash.
Remove any wash buer left at the
bottom of tube at the end of drying step.
Add 50µl of Elution Buer B to tube.
Mix by pipetting 20x and incubate for 5
mins.
Place tube on magnetic rack, wait for 5
mins and then transfer supernatant to
clean tube.
Repeat washing step with Wash Buer B1.
Place tube on magnetic rack, wait for 2
minutes and discard supernatant.
Vortex for 5 mins in a vortex shaker.
Add 400µl of Binding Bead and Binding
Buer mixture to microfuge tube and mix
by pipetting.
Mix 40µl of Binding Beads B with
400µl Binding Buer B.
For each RNA sample, prepare DNase
buer by adding 10µl of DNase prepared
in the previous step to 40µl of DNase
Reaction Buer in a microfuge tube.
Mix DNase with buer by gently inverting
the tube a few times.
Add 100µl of DNase Reaction Buer to
DNase pellet and then add 100µl of
glycerol.
Mix by gently inverting the tube.
Reconstituted pellet must be stored at -20 °C.
DNase Reaction Buer
Glycerol
Total RNA Extraction Kit
Quick Start Guide
Part DNase Treatment
26 27 2825
21
19
16
DNase Buer
Add 50µl of DNase buer to 50µl of RNA
sample in a microfuge tube.
17 Gently shake microfuge tube and
incubate at room temperature for 25
minutes.
1815
20
24
Binding Bead &
Buer Mix
Binding Buer B
1.0 
 Total RNA Extraction Kit
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GALENVS magneti User guide

magneti
Brand
GALENVS
Model
magneti
Type
User guide

GALENVS magneti is a compact, versatile, and easy-to-use nucleic acid purification system ideal for research and diagnostic applications. Its magnetic bead technology enables rapid and efficient extraction of high-quality nucleic acids from various samples, including blood, cells, tissues, plants, and bacteria. With GALENVS magneti, you can expect:

  • Fast and efficient nucleic acid extraction: The system utilizes magnetic beads to quickly capture and purify nucleic acids, reducing processing time and increasing productivity.

  • High-quality nucleic acid yield: GALENVS magneti delivers high-quality nucleic acids suitable for various downstream applications, including PCR, qPCR, sequencing, and genotyping.

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