GALENVS WW0016-12 User guide

Brand: GALENVS

Model: WW0016-12

Type: User guide

GALENVS WW0016-12 is a wastewater DNA/RNA extraction kit designed for rapid and efficient purification of nucleic acids from wastewater samples. The kit utilizes magnetic bead-based technology to selectively bind and concentrate target molecules, allowing for their easy elution in a concentrated form. This user-friendly kit is compatible with the miQron automated nucleic acid extraction system, enabling high-throughput processing of multiple samples with consistent and reliable results.

GALENVS WW0016-12 is a wastewater DNA/RNA extraction kit designed for rapid and efficient purification of nucleic acids from wastewater samples. The kit utilizes magnetic bead-based technology to selectively bind and concentrate target molecules, allowing for their easy elution in a concentrated form. This user-friendly kit is compatible with the miQron automated nucleic acid extraction system, enabling high-throughput processing of multiple samples with consistent and reliable results.

Columns 6 & 12 contains the purified

DNA/RNA elution.

8

When program is complete, remove

plate from miQron and discard combs.

Run

Report

Settings

Run 05-19-2021 11:16

WWKit

Select the WWKit protocol and press

7Insert two combs.

Place plate into the miQron, taking care

that the label is facing outward.

6

5Avoiding pellet, transfer up to 400μl of

supernatant to the Binding Buer wells

(Columns 1 & 7).

You can add up to 16 samples.

4Remove tube from magnetic rack and

resuspend beads in 400µl of Lysis Buer.

Vortex at max speed for 5 mins, then

separate beads using a 2ml magnetic rack.

Lysis Buer

3Resuspend beads in 1ml RNase-free water,

then transfer the mixture to a clean 2ml

centrifuge tube.

Place tube on 2ml magnetic rack to capture

beads, then discard supernatant.

Wash Buer #1

2Place sample on 15ml magnetic rack to

capture beads, then discard supernatant.

Remove tube from magnetic rack.

Concentration Buer

Concentration Beads

Transfer 10ml of wastewater sample to 15ml

conical tube. Add 100µl of Concentration

Buer to sample. Invert 5 times.

Add 75µl of Concentration Beads. Invert 5

times and incubate for 10 mins. Invert 3 more

times at the 5 min mark.

1

Step Name

-Load-

Bead Pickup

Binding

Wash #1

Wash #2

Elution

-Unload-

Column

2 & 8

1 & 7

3 & 9

4 & 10

6 & 12

2 & 8

Volume

(µl)

30

1000

600

600

100

Time

(sec)

60

300

60

60

300

Mixing Speed

(1–10)

Medium

Medium

Medium

Medium

Low

Dry Time

(sec)

0

0

0

120

0

Magnet Capture

Time (sec)

1 x 30 sec

5 x 30 sec

2 x 30 sec

2 x 30 sec

2 x 30 sec

Temp

OFF

OFF

OFF

OFF

65ºC

WWKit miQron protocol parameters

Binding Buer (600µl)

Columns 1 & 7

Binding magnetic

nanoparticles (30µl)

Columns 2 & 8

Elution Buer (90µl)

Columns 6 & 12

Wash #1 Buer (600µl)

Columns 3 & 9

Wash #2 Buer (600µl)

Columns 4 & 10

Elution Add Lysis

supernatant

ElutionAdd Lysis

supernatant

1.0



Wastewater DNA/RNA Extraction Kit



Wastewater DNA/RNA

Extraction Kit

Quick Start Guide

1

GALENVS WW0016-12 User guide

Brand: GALENVS

Model: WW0016-12

Type: User guide

GALENVS WW0016-12 is a wastewater DNA/RNA extraction kit designed for rapid and efficient purification of nucleic acids from wastewater samples. The kit utilizes magnetic bead-based technology to selectively bind and concentrate target molecules, allowing for their easy elution in a concentrated form. This user-friendly kit is compatible with the miQron automated nucleic acid extraction system, enabling high-throughput processing of multiple samples with consistent and reliable results.

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