GALENVS magnetic Plasmid Miniprep Kit User guide

magnetic Plasmid Miniprep Kit

Brand: GALENVS

Model: magnetic Plasmid Miniprep Kit

Type: User guide

10 11

Place microfuge tube on magnetic rack

for 1 min.

Transfer pure plasmid supernatant to a

clean microfuge tube.

9Add 50–200µl of Elution Buer and mix

well by pipetting up-down (10–15x) until

solution is homoegenous.

Incubate for 1 min.

8Repeat with 600µl of Wash Buer #2,

place on magnetic rack for 1 min.

Discard supernatant and leave to dry

for 1–5 mins.

Elution Buer

6Place microfuge tube on magnetic

rack for 1 min. Discard supernatant. 7Add 600µl of Wash Buer #1 and

mix well by pipetting up-down

(5–10x) until solution is homogenous.

Place microfuge tube on magnetic

rack for 1 min. Discard supernatant.

Add 400µl of Binding Buer to the

supernatant in the new microfuge tube

and mix well by pipetting up-down

(10-15x) until solution is homogenous.

Incubate for 1 min at room temp.

5

Place tube on magnetic rack for 1 min.

Transfer supernatant to a new microfuge

tube. Discard previous tube.

4

2Add 200µl of Lysis Buer and mix well by

pipetting up-down (5–10x) until solution is

homogenous.

Incubate for 8 mins at room temp.

Lysis Buer

3Add 100µl of Neutralization Buer and

mix well by pipetting up-down (5–10x).

Incubate for 2 mins at room temp.

Neutralization Buer

Binding Buer

Pellet 1–10ml of bacterial cell sample.

(<5ml for high-copy plasmid, <10ml for

low-copy plasmid). Discard supernatant.

Add 200µl of Resuspension Buer to

the pellet and mix well by pipetting

up-down until solution is homogenous.

1

Resuspension Buer

Wash Buer #1

Wash Buer #2

1.0

 

 Plasmid Miniprep Kit

Plasmid Miniprep Kit

Quick Start Guide

1

GALENVS magnetic Plasmid Miniprep Kit User guide

magnetic Plasmid Miniprep Kit

Brand: GALENVS

Model: magnetic Plasmid Miniprep Kit

Type: User guide

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