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magneti

GALENVS magneti User guide

  • Hello! I've reviewed the Quick Start Guide for the TRCKit RNA Extraction Kit. This guide provides a step-by-step procedure for RNA extraction and purification using the miQron device. The document details the necessary steps, including lysis, binding, washing and elution. I'm ready to assist with any questions you may have about the process and its parameters.
  • What is the first step of TRCKit protocol?
    How many samples can I add to Lysis/Binding Buffer?
    Which columns contain the purified RNA elution?
Columns 6 and 12 contain the purified
RNA elution.
5Select the TRCKit protocol and press
When program is complete, remove
plate from miQron and discard combs.
Run
Report
Settings
Run 05-19-2021 11:16
TRCKit
Insert two combs.
4
3Place plate into the miQron, taking care
that the label is facing outward.
2Add up to 16 samples to Lysis/Binding
Buer (columns 1 and 7).
Remove the protective foil.
1
Lysis / Binding
Buer (600µl)
Columns 1 & 7
Binding magnetic
nanoparticles (200µl)
Columns 2 & 8
Elution Buer (90µl)
Columns 6 & 12
Wash #1 Buer (600µl)
Columns 3 & 9
Wash #2 Buer (600µl)
Columns 4 & 10
90µl extracted
and purified
RNA elution
200µl sample
added to Lysis/
Binding Buer
90µl extracted
and purified
RNA elution
200µl sample
added to Lysis/
Binding Buer
Step Name
Lysis
Bead Transfer
Binding
Wash #1
Wash #2
Elution
Discard Comb
Column
1 & 7
2 & 8
1 & 7
3 & 9
4 & 10
6 & 12
2 & 8
Volume
(µl)
800
200
800
600
600
90
600
Time
(sec)
12
–
12
12
12
12
0
Mixing Speed
(1–10)
7
–
7
6
6
7
5
Dry Time
(sec)
0
0
0
0
12
0
0
Magnet Capture
Time (sec)
–
100
110
100
100
200
0
TRCKit miQron protocol parameters
Total RNA Extraction Kit  1.0

Total RNA Extraction Kit
Quick Start Guide
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