2. GALENVS
  3. UP0050

GALENVS UP0050 User guide

  • Hello! I've reviewed the Quick Start Guide for the GALENVS Universal Pathogen DNA/RNA Extraction Kit, including models UP0050-12 and UP0200-12. This kit utilizes magnetic nanoparticles and various buffers to purify DNA/RNA from different sample types. I'm ready to answer your questions about the provided procedure, including sample preparation, buffer usage, and magnetic separation process.
  • What should be done with wash buffers before first use?
    How should extraction bottles be prepared before use?
    What is the initial step for sample preparation?
    How much Lysis/Binding Buffer should be added?
    What is the next step after adding the Lysis/Binding Buffer?
8Place tube on magnetic rack to separate
beads (~1–2 mins). Transfer clean DNA/RNA
solution (supernatant) to clean tube.
76 Remove tube from magnetic rack and
resuspend DNA/RNA–bead complex in
50–100µl of Elution Buer.
Mix well by pipetting up-down 15–20x to
elute DNA/RNA from beads and let stand
for 1–2 mins.
Optional: elute at 60ºC for improved yield.
Elution Buer
!
5
Wash Buer #2
Repeat wash with 600µl of Wash Buer #2,
return to magnetic rack for 1–2 mins, then
discard supernatant and leave to dry for 1 min.
4Remove tube from magnetic rack and
resuspend DNA/RNA–bead complex in
600µl of Wash Buer #1.
Return to magnetic rack for 1–2 mins, then
discard supernatant.
Wash Buer #1
Place tube on magnetic rack for 1–2 mins
to capture DNA/RNA-bead complex, then
discard supernatant.
3
Lysis/Binding Buer
Add 600µl of Lysis/Binding Buer
Mix well by pipetting up-down 10–15x.
Incubate for 5 mins.
2
Binding magnetic
nanoparticles
Add 30µl of binding magnetic
nanoparticles. Mix well by pipetting
up-down 10–15x.
Incubate for 5 mins.
1In 1.5ml microfuge tube, add 100µl–200µl
of sample (swabs, serum/plasma, whole
blood, cerebrospinal fluid (CSF), stool,
sputum, exudate, urine, or saliva).
Universal Pathogen DNA/RNA
Extraction Kit
   1.1
Universal Pathogen
DNA/RNA
Extraction Kit
Quick Start Guide
!Before first use add ethanol to wash buers as per label instructions.
Before extraction mix bottles well by inverting upside down several times.
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