GALENVS Plasmid User guide

Plasmid
Brand
GALENVS
Model
Plasmid
Type
User guide
When program is complete, discard
combs then remove plate from miQron.
Columns 5 and 11 contain the purified
DNA elution.
At Stop 2, add 400µl of Binding Buer
to columns 1 and 7.
Place plate back in miQron
and continue the program.
7
6At Stop 1, add 100µl of Neutralization
Buer to columns 1 and 7.
Place plate back in miQron
and continue the program.
5Select the PMKit protocol and press
Run
Report
Settings
Run 05-19-2021 11:16
PMKit
Insert two combs.
4
3Place plate into the miQron, taking care
that the label is facing outward.
2Resuspend bacterial pellet in 200µl of
Resuspension Buer then add to Lysis
Buer (columns 1 and 7).
Remove the protective foil.
1
50µl extracted
and purified
DNA elution
200µl Resuspension
added to Lysis Buer
50µl extracted
and purified
DNA elution
200µl Resuspension
added to Lysis Buer
Wash #1 Buer (600µl)
Columns 3 & 9
Wash #2 Buer (600µl)
Columns 2 & 8, 4 & 10
Elution Buer (50µl)
Columns 5 & 11
Lysis Buer (200µl)
Columns 1 & 7
@Stop 1: Add Neutralization Buer
(100µl) to columns 1 & 7
@Stop 2: Add Binding Buer
(400µl) to columns 1 & 7
Step Name
Lysis
Stop 1 –
Neutralization
Discard MNP
Stop 2 –
Binding
Wash #1
Wash #2
Elution
Discard MNP
Column
1 & 7
1 & 7
2 & 8
1 & 7
3 & 9
4 & 10
5 & 11
2 & 8
Volume
(µl)
400
500
600
900
600
600
50
600
Time
(sec)
480
120
12
60
60
60
60
6
Mixing Speed
(1–10)
3
5
10
5
5
5
5
10
Dry Time
(sec)
0
0
0
0
0
60
0
0
Magnet Capture
Time (sec)
n/a
75
n/a
50
50
50
50
0
PMKit miQron protocol parameters
 Plasmid Miniprep Kit  1.0
Plasmid Miniprep Kit
Quick Start Guide
  • Page 1 1

GALENVS Plasmid User guide

Plasmid
Brand
GALENVS
Model
Plasmid
Type
User guide
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