GALENVS PD0016 User guide

PD0016
Brand
GALENVS
Model
PD0016
Type
User guide
Columns 6 and 12 contain the purified
DNA elution.
When program is complete, remove
plate from miQron and discard combs.
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Run 05-19-2021 11:16
PDKit
Select the PDKit protocol and press
7
Insert two combs.
6
Place plate into the miQron, taking care
that the label is facing outward.
5
4Avoiding pellets, transfer up to 400µl of
supernatant to Binding Buer (Columns 1
& 7).
You can add up to 16 samples.
Remove the protective foil.
3
Add 600µl Lysis Buer, 60µl PPB, and 5µl
RNase.
Mix for 3 mins using TissueLyser at max
speed or vortex for 10 mins; then
centrifuge at 20,000g for 2 mins.
2
20,000g
PPB
RNase
Lysis Buer
Add up to 50mg of plant leaves sample
to the lysis bead tube provided.
1
100µl extracted
and purified
gDNA elution
400µl sample
added to
Binding Buer
100µl extracted
and purified
gDNA elution
400µl sample
added to
Binding Buer
Step Name
Binding
Wash #1
Wash #1
Wash #2
Wash #2
Elution
Discard Comb
Column
1 & 7
2 & 8
3 & 9
4 & 10
5 & 11
6 & 12
2 & 8
Volume
(µl)
600
600
600
600
600
100
600
Time
(sec)
300
60
60
60
60
60
0
Mixing Speed
(1–10)
7
7
7
7
7
10
5
Dry Time
(sec)
0
0
0
0
300
0
0
Magnet Capture
Time (sec)
150
90
90
90
90
150
0
PDKit miQron protocol parameters
Wash #1 Buer (600µl)
Columns 2 & 8, 3 &9
Wash #2 Buer (600µl)
Columns 4 & 10, 5 & 11
Elution Buer (100µl)
Columns 6 & 12
Binding Buer (600µl)
Columns 1 & 7
Plant DNA Extraction Kit  1.1

Plant DNA Extraction Kit
Quick Start Guide
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GALENVS PD0016 User guide

PD0016
Brand
GALENVS
Model
PD0016
Type
User guide
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