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  3. PD0016
PD0016

GALENVS PD0016 User guide

  • Hello! I've reviewed the user guide for the Plant DNA Extraction Kit PD0016-12. This kit facilitates the extraction and purification of plant DNA using a magnetic bead-based protocol. The document outlines the steps involved including lysis, binding, washing, and elution using specific buffers. Up to 16 samples can be processed in a single run. I am ready to answer your questions about the kit and its usage.
  • How much plant sample can be used?
    What is the volume of Lysis Buffer used?
    What are the final purified DNA elutions located?
    What is the volume of Elution Buffer?
Columns 6 and 12 contain the purified
DNA elution.
When program is complete, remove
plate from miQron and discard combs.
Run
Report
Settings
Run 05-19-2021 11:16
PDKit
Select the PDKit protocol and press
7
Insert two combs.
6
Place plate into the miQron, taking care
that the label is facing outward.
5
4Avoiding pellets, transfer up to 400µl of
supernatant to Binding Buer (Columns 1
& 7).
You can add up to 16 samples.
Remove the protective foil.
3
Add 600µl Lysis Buer, 60µl PPB, and 5µl
RNase.
Mix for 3 mins using TissueLyser at max
speed or vortex for 10 mins; then
centrifuge at 20,000g for 2 mins.
2
20,000g
PPB
RNase
Lysis Buer
Add up to 50mg of plant leaves sample
to the lysis bead tube provided.
1
100µl extracted
and purified
gDNA elution
400µl sample
added to
Binding Buer
100µl extracted
and purified
gDNA elution
400µl sample
added to
Binding Buer
Step Name
Binding
Wash #1
Wash #1
Wash #2
Wash #2
Elution
Discard Comb
Column
1 & 7
2 & 8
3 & 9
4 & 10
5 & 11
6 & 12
2 & 8
Volume
(µl)
600
600
600
600
600
100
600
Time
(sec)
300
60
60
60
60
60
0
Mixing Speed
(1–10)
7
7
7
7
7
10
5
Dry Time
(sec)
0
0
0
0
300
0
0
Magnet Capture
Time (sec)
150
90
90
90
90
150
0
PDKit miQron protocol parameters
Wash #1 Buer (600µl)
Columns 2 & 8, 3 &9
Wash #2 Buer (600µl)
Columns 4 & 10, 5 & 11
Elution Buer (100µl)
Columns 6 & 12
Binding Buer (600µl)
Columns 1 & 7
Plant DNA Extraction Kit  1.1

Plant DNA Extraction Kit
Quick Start Guide
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