Roche Cedex HiRes User manual

Cedex HiRes Analyzer Operator’s Manual

Software Version 2.4

October 2020

How to Use the Cedex HiRes Analyzer Guides

Before reading, please review all Addenda for important information.

Addenda

Provide updates to the Cedex HiRes Analyzer Guides, including new supplementary information and

corrections to previous editions.

User Training Guide

Provides detailed step-by-step instructions for routine operation using the main applications of

the Cedex HiRes Analyzer, including instrument startup and shutdown.

Quick Guide

Provides a short set of instructions for use in the laboratory, describing the basic handling steps.

This shorter form of information is for routine use after you are familiar with the details of the Cedex

HiRes Analyzer described in the User Training Guide.

Operator´s Guide

Provides a detailed description of the Cedex HiRes Analyzer, system components and all relevant

software information not covered by the User Training Guide. For safety and installation

requirements, always refer to the Operator´s Guide.

Cedex HiRes Analyzer User Training Guide

Software Version 2.4

October 2020

3

Table of Contents

Prologue 5

I Document Information .................................................................................................................................................. 5

1 Revision History .................................................................................................................................................................... 5

2 Edition Notice ........................................................................................................................................................................ 5

3 Copyright ................................................................................................................................................................................. 5

4 Trademarks ............................................................................................................................................................................. 5

II Contact Addresses .......................................................................................................................................................... 6

III. Declaration of Conformity ........................................................................................................................................... 6

IV Intended Use ....................................................................................................................................................................... 6

V Preamble ............................................................................................................................................................................... 6

VI Conventions Used in this Guide ............................................................................................................................... 7

VII Warnings and Precautions .......................................................................................................................................... 8

A Startup 10

1 Turning on the Cedex HiRes Analyzer, Control Unit, and Software ..................................................11

2

Preparing the Cedex HiRes Analyzer for Measurements .............................................................................. 12

2.1 Working with the Multi Sampler ..................................................................................................................................12

2.2 Installing a Reagent Kit ...................................................................................................................................................13

2.3 Perform a Prime .................................................................................................................................................................. 14

B Main Application: Counting Cells and Determining Viability 15

1 Check the Analyzer Before Starting Measurements ..................................................................................15

2 Prepare the Samples ....................................................................................................................................................15

3 Setting Up a Single Measurement in the Measurement Dialog Box ................................................16

3.1 Open the Measurement Dialog Box and Deﬁne the Sample Parameters .................................................... 16

3.2 Deﬁne the Measurement and Analysis Parameters .............................................................................................17

4 Starting the Measurement ........................................................................................................................................ 18

5 End of Measurement ....................................................................................................................................................19

6 Viewing the Results in the Measurement Dialog Box ...............................................................................20

6.1 Viewing the Results in the Measurement Dialog Box .........................................................................................20

6.2 Viewing the Results in the Histograms ......................................................................................................................22

6.3 Viewing the Images ...........................................................................................................................................................22

7 Setting up Multiple Measurements Using the Multi Run Dialog Box ..............................................23

7.1 Open the Multi Run Dialog Box and Prepare Samples for Analysis ..............................................................23

7.2 Deﬁne the Sample Parameters and the Measurement and Analysis Parameters

in the Multi Run Dialog Box...........................................................................................................................................24

7.3 Schedule Samples and Start the Measurement ....................................................................................................25

7.4 Interrupting a Multi Run ..................................................................................................................................................26

7.5 Results ...................................................................................................................................................................................26

7.6 The Multi Run Result List................................................................................................................................................27

8 Measurement List ..........................................................................................................................................................28

4

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

Table of Contents

C Additional Applications 29

1 Monitoring Growth of Cells Over Time ...............................................................................................................29

1.1 Performing Measurements for Growth Curve Analysis .......................................................................................29

1.2 Result Analysis ....................................................................................................................................................................29

1.2.1 Create a CTC View .............................................................................................................................................................30

1.2.2 Add One or More Series of Measurements to the CTC View ...........................................................................31

1.2.3 Create a CTC Chart Associated with the View to Analyze Pattern of Growth Over Time ....................... 34

1.2.4 Exponential Regression Function to Determine the Speciﬁc Growth Rate

and Doubling Time of the Cell Culture ......................................................................................................................36

1.2.5 Checking Regression Curve Fitting .............................................................................................................................39

1.2.6 Editing Measurements in a View .................................................................................................................................40

2 Analyzing Unusual Cells .............................................................................................................................................41

2.1 Overview ................................................................................................................................................................................ 41

2.2 Example of Cells Not Recognized by the Cell Type Std. Size ............................................................................42

2.2.1 Create a New Cell Type ...................................................................................................................................................43

2.2.2 Modify the Parameters for the New Cell Type via the Live Operator Dialog Box ......................................45

2.2.3 Reprocess the Measurement Using the New Cell Type ......................................................................................48

D Shutting Down the Cedex HiRes Analyzer 50

1 Perform an LM Shutdown..........................................................................................................................................50

2 Log Out ................................................................................................................................................................................. 50

3 Shut Down the Cedex Control Center ................................................................................................................51

E Index 52

5

Prologue

I Document Information

1 Revision History

Version Software Version Revision Date Changes

1.0 V2.3 August 2013 First version

2.0 V2.3.2 April 2014 Updated Intended Use statement

Updated for SW version 2.3.2

3.0 V2.4 October 2017 Completely revised version due to

Software update, SW version 2.4

3.1 V2.4 October 2020 Safety statement about sharps, rough

edges, and/or moving parts added to

Chapter XI, Warnings and Precautions.

Editorial changes.

2 Edition Notice

The Cedex HiRes Analyzer User Training Guide, Software version 2.4, is for users of the Cedex HiRes Analyzer

together with the Cedex HiRes Analyzer Operator’s Guide for Software Version 2.4.

Every effort has been made to ensure that all the information contained in the Cedex HiRes Analyzer User

Training Guide is correct at the time of printing. However, Roche Diagnostics GmbH reserves the right to make

any changes necessary without notice as part of ongoing product development.

3 Copyright

Information in this document is subject to change without notice. No part of this document may be reproduced

or transmitted in any form or by any means, electronic or mechanical, for any purpose, without the express

written permission of Roche Diagnostics GmbH.

Questions or comments regarding the contents of this User Training Guide can be directed to your local Roche

Diagnostics representative.

4 Trademarks

CEDEX is a trademark of Roche.

All other product names and trademarks are the property of their respective owners.

Document Information

6

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

II Contact Addresses

Manufacturer Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

Distribution Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

Distribution in USA Roche Diagnostics

9115 Hague Road

Indianapolis, Indiana

III. Declaration of Conformity

Instrument

approvals

The Cedex HiRes Analyzer meets the protection requirements laid down in:

c Directive 2014/30/EU of the European Parliament and Council of 26. February 2014 relating to

electromagnetic compatibility (EMC).

c Directive 2014/35/EU of the European Parliament and Council of 26. February 2014 relating to

electrical equipment designed for use within certain voltage limits.

c Directive 2011/65/EU of the European Parliament and of the Council of 8 June 2011 on the

restriction of the use of certain hazardous substances in electrical and electronic equipment.

Compliance with the applicable directive(s) is provided by means of the Declaration of Conformity.

Regulatory compliance is demonstrated by the following marks:

c Complies with the provisions of the applicable EU directives.

c RoHS (Restriction of Hazardous Substances) compliant from Serial No. G037E0001.

Issued by Underwriters Laboratories, Inc. (UL) for Canada and the US. ‘Laboratory Equipment’ is

the product identiﬁer as shown on the type plate.

IV Intended Use

The Cedex HiRes Analyzer automatically determines the cell concentration and viability of cell suspensions. It

must be used exclusively by laboratory professionals who are trained in laboratory techniques and have studied

the Instructions for Use of this system.

The Cedex HiRes Analyzer is for use in quality control/manufacturing process only.

It was neither developed nor validated by the manufacturer for any kind of in vitro diagnostic application.

V Preamble

Before setting up operation of the Cedex HiRes Analyzer, it is important to read this User Training Guide and

the Cedex HiRes Analyzer Operator’s Guide thoroughly and completely. Non-observance of the instructions

contained herein or performing an operation not stated in this Training Guide could cause safety hazards.

Contact Addresses

7

Prologue

Conventions Used in this Guide

VI Conventions Used in this Guide

Text Conventions

To present information consistently and memorably, the following text conventions are used in this Training Guide:

Numbered list

Steps in a procedure that must be performed in the order listed.

Italic type

Used for operating instructions for the Cedex HiRes Software. In addition, important notes

and information notes are shown in italic type.

Blue italic type

Refers to a different section in this Training Guide or the Cedex HiRes Analyzer Operator’s

Guide, which should be consulted.

Symbols

In this Training Guide, the following symbols are used as visual signals:

Symbol Heading Description

WARNING This symbol is used to indicate that non-compliance with instructions or

procedures could lead to physical injury or even death or could cause

damage to the system.

IMPORTANT NOTE Information critical to the success of the procedure or use of the product.

INFORMATION NOTE Additional information about the current topic or procedure.

ccc

Procedure continued on next page.

n

End of procedure.

The following symbols appear on the analyzer:

Symbol Heading Description

CE MARK

The CE mark on the instrument type plate indicates conformity with

requirements of the directives relevant for this instrument.

cUL MARK On the instrument type plate.

CAUTION On the instrument type plate. Consult the accompanying documents for

important safety-related information such as warnings and precautions.

8

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

VII Warnings and Precautions

Moving parts may cause hand injuries. Never use the Cedex HiRes Analyzer without the protective cover

on the Multi Sampler and Syringe Module.

Good Laboratory Practice can reduce the risk of injury. Be aware of your laboratory environment, well-

prepared, and follow the instructions for use. Some areas of the instruments may have sharps, rough edges,

and/or moving parts. Wear personal protective equipment to minimize the risk of injury from bodily contact

with such parts, especially in less accessible areas, or while cleaning the instrument. Your personal protective

equipment should be appropriate to the degree and type of potential hazard, e. g. suitable lab gloves, eye

protection, lab coat, and footwear.

It is important that the following safety instructions and guidelines be observed in order to ensure safe and

reliable operation of the Cedex HiRes Analyzer.

In an emergency, immediately turn the power switch off and unplug the instrument.

c Place the device on a dry and sturdy surface, free of vibrations.

c Avoid transporting the device once it has been assembled.

c Prevent the device from getting wet.

c Protect the device from dust and smoke.

c Do not expose the device to direct sunlight.

c Ensure there is sufﬁcient air circulation around the Cedex HiRes Analyzer during operation (particularly

around the Control Unit and monitor).

c Operate within a constant temperature range of 10°C to 40°C (50°F to 100°F). Optimal image quality is

achieved between 20°C and 30°C.

c For best performance, it is recommended to keep the Cedex HiRes Analyzer permanently on and only shut

down the Software application on a regular basis. The Analyzer needs at least 2 hours to warm up before

it can be used for measurements.

c The Cedex HiRes Analyzer should only be used with the supplied or speciﬁed devices.

c Only trained personnel in a controlled laboratory environment should operate the Cedex HiRes Analyzer.

c All services and repairs should be performed only by an authorized Roche Service representative.

c Always ensure that there are sufﬁcient reagents in the reagent containers.

c Clean the containers before reusing them.

c Always observe the safety regulations when handling Trypan Blue, Detergent, Cleaning Solution, or acids

and bases.

c Waste contaminated with tissue or cells, for example, Cedex Sample Cups, pipette tips, etc., must be

disposed of according to the recommended rules for handling biohazard materials.

c Any leaks in the Liquid Management system must be repaired immediately. Contact your distributor or

authorized Roche Service representative.

c Always ensure that there is a Cedex Sample Cup attached to the single sample port or placed in the

DefaultCup position on the Multi Sample Tray.

c Only use Cedex Detergent for the cleaning process in the Liquid Management system.

c Only use Cedex Sample Cups from Roche Diagnostics for the conveyance of cell samples to the analyzer.

Other sample cups can cause irreparable damage to the Cedex HiRes Analyzer.

Warnings and Precautions

9

Prologue

c Always wear protective clothing, particularly gloves, when handling samples.

c Particles (especially microcarriers) larger than 90 μm should not gain access into the analyzer because they

cause irreparable damage and render the device non-functional.

c Dirt or dust particles should not gain access into the device.

c Ensure that all equipment required for sample preparation, for example, Cedex Sample Cups, pipette

tips, etc., are stored in a dust-free environment since coarse dust particles can impair the ﬂow chamber’s

functionality.

c Check that the Cedex Sample Cup is free of contamination before use. Only clean Cedex Sample Cups can

guarantee optimal functioning of the Cedex HiRes Analyzer.

c Ensure that the Trypan Blue solution is free of contamination, otherwise ﬁlter prior to use. Use the Cedex

HiRes Reagent Kit for best results.

c Secure your measurement data via regular backups of the database.

The manufacturer is not responsible or liable for any damages resulting from improper use or failure to

comply with the regulations in the Operating Instructions.

Measurements that have been migrated from older software database versions may have a different nomen-

clature with regard to certain information (e.g., Cell Type names or Precision names) than those described

in this guide. The settings described in this guide apply to measurements that have been performed with

Software version 2.4.

The Software and hardware of your Cedex HiRes Analyzer can differ from the information described here,

however this will not make a difference to the operational functions.

Refere to the Cedex HiRes Analyzer Operator’s Guide for further information about warnings and precautions.

Warnings and Precautions

10

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

A

A Startup

Before starting the Cedex HiRes Analyzer, ensure that the system is installed properly. Information about the in-

stallation of the Cedex HiRes Analyzer is provided in Section B, System Description, in the Cedex HiRes Analyzer

Operator’s Guide.

Cedex HiRes Analyzer hardware components must be turned on before starting the analysis Software.

Otherwise, the analysis Software may not be correctly initialized.

Remote Port

Multi Sampler

Syringe module with 8-way valve

Tray with the Cedex HiRes Reagent Kit

ON/OFF Switch

Multi Sample Tray for

Cedex Sample Cups

11

Startup

A

1 Turning on the Cedex HiRes Analyzer, Control Unit,

and Software

1

Press the ON/OFF switch on the Cedex HiRes Analyzer.

The switch lights up with a blue light indicating that the Cedex HiRes Analyzer is on:

ON/OFF Switch

2

Turn on the Control Unit (PC) and the monitor.

3

The Windows operating system starts, and a prompt for a username and password appears on the

Control Unit.

Log in as Win-Admin, password Win-Admin unless a different Windows operating system account has

been set up.

4

Check that the Cedex Server Software has already automatically started; it may appear as a minimized

dialog box in the task bar at the bottom of the screen.

If it has not started, double-click on the Cedex Server icon on the desktop to start the server.

5

Double-click the Cedex Client icon on the desktop to start the Cedex HiRes Software.

The Cedex Server must be started before the Cedex Client can be started.

6

The Log In dialog box of the Cedex HiRes Analyzer program opens.

Enter the username and password in the respective ﬁelds. Factory installed default user is Superuser

(password spass).

Click on the OK button to log in or press <Return>.

The Cedex Control Center opens.

n

The ﬁrst time a Cedex HiRes Analyzer is set up or when the Cedex HiRes Analyzer is moved to a new location,

a focus adjustment must be performed. See Automatic Adjustment and Alignment Veriﬁcation in the Cedex

HiRes Analyzer Operator’s Guide.

Turning on the Cedex HiRes Analyzer, Control Unit, and Software

12

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

A

2

Preparing the Cedex HiRes Analyzer for Measurements

2.1 Working with the Multi Sampler

Moving parts of the Multi Sampler may cause hand injuries. The Multi Sampler needle may cause

injuries during operation. Never use the Cedex HiRes Analyzer without the protective cover in place for the

Multi Sampler.

The Multi Sampler is part of the Cedex HiRes Analyzer, and automatically conveys up to 20 cell suspension

samples in sequence to the Cedex HiRes Analyzer for analysis.

Inserting the Multi Sample Tray

To insert the Multi Sample Tray, gently insert the tray into the notch until the automatic tray rope starts pulling

it into the Analyzer, as shown:

Ejecting the Multi Sample Tray

To eject the Multi Sample Tray, gently push on the right-hand corner of the tray when the system is idle. The

tray cannot be ejected if the system is moving or the needle is down in a cup.

Press to eject

The Multi Sampler DefaultCup

A Cedex Sample Cup is required for performing liquid management routines, such as Prime, LM Shutdown,

calibration, and cleaning routines. The position of the Cedex Sample Cup used for all liquid management

routines is called the DefaultCup position. The standard (default) position for the DefaultCup is position 1

of the Multi Sample Tray. The DefaultCup position can be changed (see Modifying the Standard Setting for the

Multi Sampler DefaultCup in the Cedex HiRes Analyzer Operator’s Guide).

Preparing the Cedex HiRes Analyzer for Measurements

Working with the Multi Sampler

13

Startup

A

2.2 Installing a Reagent Kit

1

Perform an LM Shutdown if the Liquid Management system has been primed

(see Section D, Perform an LM Shutdown).

2

Open the Reagent Kit chamber by pushing the front cover upwards until it clicks into place.

3

Remove the old Reagent Kit with the tray, and dispose of the old reagent bottles.

4

Open the new Cedex HiRes Reagent Kit, and remove the covers from the individual containers.

5

Place the Reagent Kit containers into the appropriate positions in the Reagent Tray:

Detergent Container

Trypan Blue Container

Cleaning Solution Container

Waste Container

Distilled or Deionized Water Container

6

Close the front cover by pulling it all the way down. When the front cover is closed, the capillaries

are automatically correctly positioned in the individual containers.

7

Select the Reagent Kit Status option in the Functions menu of the Cedex Control Center;

the Reagent Kit Status dialog box opens:

8

Click the REPLACE ALL CONTAINERS button in the right-hand corner of the Reagent Kit Status dialog box to

conﬁrm the changing of the Reagent Kit. The Reagent Control display at the bottom right-hand corner of the

Cedex Control Center turns from red to green and indicates the number of runs left.

ccc

Preparing the Cedex HiRes Analyzer for Measurements

Installing a Reagent Kit

14

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

A

9

Place a clean Cedex Sample Cup in the DefaultCup position on the Multi Sample Tray or attach it to the

single sample port, for a system with a single sample port.

Perform a Prime (see Perform a HW Startup and Prime, below).

For a system with a single sample port, ensure that a Cedex Sample Cup is always attached to the single

sample port. The Cedex Sample Cup (4 mL) is correctly attached when it is in an upright position beneath

the single sample port, and the capillary tube almost reaches the bottom of the Cedex Sample Cup:

n

2.3 Perform a Prime

Prime ﬁlls the capillary tubes and 8-way valve with the appropriate reagents. HW Startup initializes hardware

interfaces such as the scanner, Multi Sampler, and pump. By default, the software automatically performs an

HW Startup when launched (see Automatically Start the Hardware Management in the Cedex HiRes Analyzer

Operator’s Guide).

Both the Prime and the HW Startup functions are located in the Hardware Management dialog box.

1

Click the HWM button in the Cedex Control Center or select Hardware Management under the Functions

menu. The Hardware Management dialog box opens.

2

Ensure that a Cedex HiRes Reagent Kit is installed (see Installing a Reagent Kit) and a clean, empty Cedex

Sample Cup is correctly positioned on the Multi Sample Tray in the DefaultCup position or attached to the

single sample port.

3

Click the PRIME button in the Hardware Management dialog box. The duration of a Prime procedure is

displayed in the Estimated total time area. The message “HW started and LM primed” appears in the current

status ﬁeld when the Prime is completed.

If the Hardware Management has not been started, the message “HW not started and LM not primed”

appears in the current status ﬁeld. Click on the HW STARTUP button if this message appears.

4

Click on

to close the dialog box.

n

There should not be a break of more than 2 hours between a Prime and a measurement. If more than 2 hours

have passed since the last measurement, perform a new Prime.

Preparing the Cedex HiRes Analyzer for Measurements

Perform a HW Startup and Prime

15

Main Application: Counting Cells and Determining Viability

B

B Main Application:

Counting Cells and Determining Viability

1 Check the Analyzer Before Starting Measurements

Accurate measurement results obtained with the Cedex HiRes Analyzer depend on correctly ﬁlled reagent

containers and the proper performance of the Analyzer. Before starting measurements, perform the following

checks:

1

Check the syringe module (2.5 mL syringe and 8-way valve) for wear and tear or contamination.

Renew the syringe if required (see Syringe Maintenance in the Cedex HiRes Analyzer Operator’s Guide).

2

Check the number of runs left. This is listed in the right-hand corner of the Control Center next to a green

or red circle.

3

Check whether there is an empty Cedex Sample Cup in the DefaultCup position on the Multi Sample Tray

or attached to the sample port, for a single sample port.

4

Perform a Prime.

n

2 Prepare the Samples

1

Transfer 300 µl of a well-mixed cell suspension, with a cell concentration in the range of between

5 × 10

4

to 1 × 10

7

cells/mL, to a clean Cedex Sample Cup.

The Cedex HiRes Analyzer tolerates volume variations of +/- 10%. Any greater variation in sample volume

will lead to inaccurate results.

2

Place the prepared Cedex Sample Cup containing the cell suspension in the appropriate cup position on

the Multi Sample Tray or attach to the sample port.

n

Check the Analyzer Before Starting Measurements

16

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

B

3 Setting Up a Single Measurement in the

Measurement Dialog Box

To set up a measurement in the Measurement dialog box:

c Open the Measurement dialog box.

c Deﬁne the Sample Parameters in the Sample Parameters group.

c Deﬁne the Measurement and Analysis Parameters in the Processing Parameters group.

3.1 Open the Measurement Dialog Box and Deﬁne the Sample Parameters

1

In the Cedex Control Center shortcuts area, click on the MEASURE button. The Measurement dialog box opens:

2

In the Sample Parameters group of the Measurement dialog box, enter a Reactor ID by either selecting

a name from the drop-down list or entering a new Reactor ID. The Reactor ID describes the sample

(e.g., the name of the cell culture) and consists of up to 20 alphanumeric characters.

3

Enter a Sample ID (e.g., P001). Select a name from the drop-down list or enter a new Sample ID.

A Sample ID consists of up to 20 alphanumeric characters.

4

If you wish, select a date from the Sample Drawing list. The sample drawing date indicates when the sample

was drawn. An additional date and time (the process time) is automatically added by the Software reﬂecting

the actual date and time the sample was measured.

5

Choose the appropriate dilution factor from the Dilution list. For undiluted samples, enter a dilution factor of 1:1.

The dilution ratio chosen from the list only relates to the manual dilution of the sample prior to placing

the sample on the system. For undiluted samples, enter a dilution factor of 1:1. When a dilution of 1:2

is prepared, then 1 part cell suspension has been added to 1 part dilution medium. A dilution of 1:3

means that 1 part cell suspension has been added to 2 parts dilution medium. The dilution factor will be

automatically taken into account in the results.

6

Select the sample volume of 300 µl in the Volume drop-down menu.

7

(Optional) Enter comments on the sample in the Comments text ﬁeld.

n

Setting Up a Single Measurement in the Measurement Dialog Box

Open the Measurement Dialog Box and Deﬁne the Sample Parameters

17

Main Application: Counting Cells and Determining Viability

B

3.2 Deﬁne the Measurement and Analysis Parameters

1

Manually enter the cup position for the sample in the Cup Position ﬁeld if there is a Multi Sampler.

The positions are marked on the Multi Sample Tray.

2

In the Processing Parameters group of the Measurement dialog box, select the desired precision level in the

Precision list.

The precision level determines the volume to be analyzed. A higher analyzed volume requires a somewhat

longer measurement time but more cells will be counted:

c Minimum: approximately 3 images are used for the analysis.

c Normal: approximately 6 images are used for the analysis.

c Superior: approximately 8 images are used for the analysis.

c Maximum: approximately 11 images are used for the analysis.

3

Select the Cell Type that optimally recognizes your cells from the Cell Type list. The following Cell Types

are available by default:

c Min. Size for small cells with a diameter of about 6 µm, such as lymphocytes; default sedimentation

time is 90 seconds.

c Std. Size for normal-sized cells with a diameter of about 12 µm, such as HB58; default sedimentation

time is 60 seconds.

c Max. Size for large cells of more than 16 µm, such as K-562; default sedimentation time is 60 seconds.

If a new Cell Type has been created, it can also be selected from the same Cell Type drop-down list.

Do not select the Cell Type option Illumination Test. This Cell Type is designed for service purposes

only and is not intended for analysis of cells. The parameters for this Cell Type should not be changed.

4

The Cedex Control Center automatically adds the:

c System Name

c Process Time

5

Prepare the sample and place the Cedex Sample Cup containing the sample in the appropriate position on

the Multi Sample Tray or attach it to the single sample port.

The Sample Port area indicates which type of sample port is used for the measurement, based on the

instrument conﬁguration (see the Cedex HiRes Analyzer Operator’s Guide for more information about the

instrument conﬁguration):

c Multi Sampler: use the Multi Sample Tray.

c Single Port: use the single sample port.

c

Remote Port: use the Remote Sample Port located at the top of the instrument (see Chapter A).

c

Custom Port: Consult with your Cedex administrator for more information about which sample

port to

use before running any measurements.

n

Setting Up a single Measurement in the Measurement Dialog Box

Deﬁne the Measurement and Analysis Parameters

18

Cedex HiRes Analyzer Software Version 2.4 - Training Guide

B

4 Starting the Measurement

1

Ensure that the Cedex Sample Cup is correctly placed in the Multi Sample Tray or attached to the single

sample port.

2

Ensure that the reagent containers are sufﬁciently ﬁlled and in their correct positions.

3

Ensure that the waste container is empty.

4

Click on the START MEASUREMENT button in the middle of the Measurement dialog box to begin the

measurement.

n

The sample is injected into the chamber, and the cells are allowed to settle to the bottom of the ﬂow chamber

for a speciﬁed amount of time (the sedimentation phase). The system checks the ﬂow chamber for impurities

with a separate scan (Prescan) during the sedimentation phase.

Starting the Measurement

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