Roche Cedex HiRes Reference guide

Brand: Roche

Model: Cedex HiRes

Type: Reference guide

Roche Cedex HiRes is a high-resolution cell analyzer designed to provide accurate and reliable cell counting and analysis in a variety of applications. It features advanced image analysis technology that enables precise cell recognition and counting, even for small or difficult-to-distinguish cells.

With its user-friendly software and intuitive interface, the Cedex HiRes allows for easy setup and operation, making it accessible to users with varying levels of expertise. The system's ability to measure a wide range of cell types and concentrations, along with its precise dilution and priming functions, ensures accurate results for both undiluted and diluted samples.

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For use in quality control/manufacturing process only.

Performing a Measurement — (November 2019)

Moving parts of the Multi Sampler may cause hand injuries. The Multi Sampler needle

may cause injuries during operation. Never use the Cedex HiRes Analyzer without the

protective cover in place for the Multi Sampler.

Start the Cedex HiRes Analyzer and Software

Turn on the Cedex HiRes Analyzer.

Turn on the monitor and PC.

Start the Cedex Software by double-clicking on the Cedex Client icon located on the

desktop (the Cedex Server should already be automatically started. It must be ﬁrst started

before the Cedex Software can be started). The Log In dialog box of the Cedex HiRes

Software will open.

Enter the username and password and press <Return>. The Cedex Control Center

appears after a successful login.

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Perform a Hardware Startup and Prime

Click on the HWM button in the Cedex Control Center to open the Hardware Management

dialog box.

If the Hardware Management has not been started, the current status ﬁeld will

have the message “Not started”.

Click on HW Startup if the Hardware Management has not been started. The Hardware

Management will start and the current status ﬁeld will have the message “HW started and

LM not primed”.

Ensure that a clean, empty Cedex Sample Cup is correctly positioned on the Multi Sample

Tray in the DefaultCup position (usually sample cup position 1), and click on Prime in the

Hardware Management dialog box. The status ﬁeld will have the message “HW started and

LM primed”.

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Perform a single measurement

Click on MEASURE in the Cedex Control Center to open the Measurement dialog box.

In the Measurement dialog box, enter a clearly deﬁned Reactor ID.

You may either

choose a

Reactor ID from the drop-down list or enter a new Reactor ID.

Enter a clearly deﬁned Sample ID. Multiple Sample IDs can be listed under one Reactor ID.

The Sample ID can be freely deﬁned.

Choose the appropriate dilution factor from the Dilution drop-down list. For undiluted

samples, enter a dilution factor of 1:1. The result will be calculated for the undiluted sample.

Enter comments in the Comment box.

Enter the sample cup position in the Cup Position dialog box.

Select the cell type that optimally recognizes your cells from the Cell Type drop-down list:

c Min. Size: small cells with a diameter of about 6 µm.

c Std. Size: normal sized cells with a diameter of about 12 µm.

c Max. Size: large cells of more than 16 µm.

If a new cell type has been created, it can also be selected from the same Cell Type

drop-down list.

Do not select the Cell Type option Illumination Test. This Cell Type is designed for

service purposes only and is not intended for analysis of cells.

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Cedex HiRes Software 2.5 Quick Guide

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112019

For use in quality control/manufacturing process only.

CEDEX is a trademark of Roche.

All other product names and trademarks are the property of their respective owners.

Performing a Measurement — (November 2019)

Select the desired precision level in the Precision drop-down list:

c Minimum: 3 images are used for the analysis.

c Normal: 6 images are used for the analysis.

c Superior: 8 images are used for the analysis.

c Maximum: 11 images are used for the analysis.

Prepare the sample. The Cedex HiRes Analyzer requires a volume of 300 µL +/- 10%.

Any greater variation in sample volume will lead to inaccurate results.

Place the Cedex Sample Cup containing the sample in the appropriate

sample cup position

on the Multi Sample Tray.

Click on the START MEASUREMENT button to start the measurement.

When the measurement is ﬁnished, the top section of the Result Data area in the

Measurement dialog box displays the overall results from the measurement.

The bottom section displays, in succession, the results from each image taken.

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Logging out and/or shutting down

Open the Hardware Management dialog box (HWM button on the Cedex Control Center).

Click on LM Shutdown to shut down the Liquid Management system.

Click on the LOGOUT button on the Cedex Control Center to log out of the Cedex Control

Center. The Cedex Control Center software program will be ended, and the Login dialog

box will

reappear.

Alternatively, shut down the software by selecting Exit under the System menu or by clicking

to close the Cedex Control Center dialog box.

When the security query “Do you really want to exit the Cedex Control Center?“ appears,

click on Yes to shut down the program.

A sample cup should always be left in the DefaultCup position on the Multi Sample Tray

(see Quick Guide “Working with the Multi Sampler”).

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Cedex HiRes Software 2.5 Quick Guide

Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

Roche Cedex HiRes Reference guide

Roche Cedex HiRes Reference guide

Roche Cedex HiRes Reference guide

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