Roche Cedex HiRes Reference guide

Brand: Roche

Model: Cedex HiRes

Type: Reference guide

Roche Cedex HiRes is an automated cell counter and analyzer that provides accurate and reliable cell counting and analysis for various applications in life science research and biopharmaceutical manufacturing. With its advanced technology, the Cedex HiRes offers:

High-Resolution Imaging: Utilizes high-resolution digital imaging to capture detailed images of cells, enabling accurate cell counting and classification.
Automated Cell Counting and Analysis: Automates the process of cell counting and analysis, providing consistent and reproducible results. This eliminates manual counting errors and streamlines the workflow.

High-Resolution Imaging: Utilizes high-resolution digital imaging to capture detailed images of cells, enabling accurate cell counting and classification.
Automated Cell Counting and Analysis: Automates the process of cell counting and analysis, providing consistent and reproducible results. This eliminates manual counting errors and streamlines the workflow.

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For use in quality control/manufacturing processes only.

Performing a Measurement — (April 2014)

Moving parts of the Multi Sampler may cause hand injuries. The Multi Sampler needle

may cause injuries during operation. Never use the Cedex HiRes Analyzer without the

protective cover in place for the Multi Sampler.

Start the Cedex HiRes Analyzer and software

Switch on the Cedex HiRes Analyzer.

Switch on the monitor and PC.

Start the Cedex Software 2.3 by double clicking on the Cedex Client icon located on the

desktop (the Cedex Server should already be automatically started. It must be ﬁrst started

before the Cedex Software 2.3 can be started). The Log In dialog box of the Cedex HiRes

Software 2.3 program will open.

Enter the username in the User Name ﬁeld and password in the Password ﬁeld and press

<Return>. The Cedex Control Center appears after a successful login.

■

Perform a Hardware Startup and Prime

Click on the HWM button in the Cedex Control Center to open the Hardware Management

dialog box.

If the Hardware Management has not been started, the current status ﬁeld will

have the message “Not started”.

Click on HW Startup if the Hardware Management has not been started. The Hardware

Management will start and the current status ﬁeld will have the message “HW started and

LM not primed”.

Ensure that a clean, empty Cedex Sample Cup is correctly positioned on the Multi Sample

Tray in the DefaultCup position (usually sample cup position 1), or on the single sample port,

and click on Prime in the Hardware Management dialog box.

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Carry out a single measurement

Click on the MEASURE button in the Cedex Control Center to open the Measurement

dialog box.

In the Measurement dialog box, enter a clearly deﬁned Reactor ID to describe the sample

(e.g. the name of the cell culture or bioreactor name) in the Reactor ID area. You may either

choose a Reactor ID from the drop down list or enter a new Reactor ID.

Enter a clearly deﬁned Sample ID in the Sample ID area. Multiple Sample IDs can be listed

under one Reactor ID. The Sample ID can be freely deﬁned.

Choose the appropriate dilution factor from the Dilution drop-down list. For undiluted

samples, enter a dilution factor of 1:1. When a dilution of 1:2 is prepared, then

1 part cell

suspension has been added to 1 part dilution medium. A dilution of 1:3 means that 1 part

cell suspension has been added to 2 parts dilution medium. The dilution factor will be

automatically taken into account in the results.

Select the sample volume of 300 µL in the Volume drop-down menu.

Enter comments (if desired) in the Comment box.

Select the sample port type in the Sample Port drop-down list:

c Auto Sampler or Multi Sampler: for systems with a Multi Sampler.

c Single: for systems with a single sample port.

Enter the cup position for the sample in the Cup Position dialog box if there is a Multi

Sampler.

Select the cell type that optimally recognizes your cells from the Cell Type drop-down list:

c Min. Size: small cells with a diameter of about 6 µm.

c Std. Size: normal sized cells with a diameter of about 12 µm.

c Max. Size: large cells of more than 16 µm.

If a new cell type has been created, it can also be selected from the same Cell Type

drop-down list.

Do not select the Cell Type option HR-Illumination, if it is available. This Cell Type is

designed for service purposes only and is not intended for analysis of cells.

ccc

Cedex HiRes Software 2.3.2 Quick Guide

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07267070001

042014

For use in quality control/manufacturing processes only.

CEDEX is a trademark of Roche.

All other product names and trademarks are the property of their respective owners.

Performing a Measurement — (April 2014)

Select the desired precision level in the Precision drop-down list:

c Minimum: 3 images are used for the analysis.

c Normal: 6 images are used for the analysis.

c Superior: 8 images are used for the analysis.

c Maximum: 11 images are used for the analysis.

Prepare the sample. The Cedex HiRes Analyzer requires a volume of 300 µL +/- 10%.

Any greater variation in sample volume will lead to inaccurate results.

Attach the Cedex Sample Cup containing the sample to the sample port, or place it in the

appropriate

sample cup position on the Multi Sample Tray. The sample cup is correctly

attached to the

single sample port when it is in an upright position directly beneath it and

the capillary tube almost reaches the base of the sample cup.

Click on the START MEASUREMENT button to start the measurement.

When the measurement is ﬁnished, the top section of the Result Data area in the

Measurement dialog box will display the overall results from the measurement. The bottom

section displays, in succession, the results from each image taken.

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Logging out and/or shutting down

Open the Hardware Management dialog box (HWM button on the Cedex Control

Center).

Click on LM Shutdown to shut down the Liquid Management system.

Click on the LOGOUT button on the Cedex Control Center to log out of the Cedex Control

Center. The Cedex Control Center software program will be ended, and the login dialog

box will

reappear.

Alternatively, shut down the software by selecting Exit under the System menu or by

clicking on to close the Cedex Control Center dialog box.

The security query, “Do you really want to exit the Cedex Control Center?“, will appear.

Click on Yes to shut down the program.

In the case of a Multi Sampler, a sample cup should always be left in the DefaultCup position

on the Multi Sample Tray (see Quick Guide “Working with the Multi Sampler”). In the case of

a single sample port, the sample cup from the last measurement should remain attached to

the sample port during the entire shutdown procedure and should only be removed when a

new sample cup has been attached for the next measurement.

■

Published by:

Roche Diagnostics GmbH

Sandhofer Strasse 16

68305 Mannheim

Germany

Cedex HiRes Software 2.3.2 Quick Guide

Roche Cedex HiRes Reference guide

Brand: Roche

Model: Cedex HiRes

Type: Reference guide

High-Resolution Imaging: Utilizes high-resolution digital imaging to capture detailed images of cells, enabling accurate cell counting and classification.
Automated Cell Counting and Analysis: Automates the process of cell counting and analysis, providing consistent and reproducible results. This eliminates manual counting errors and streamlines the workflow.

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Roche Cedex HiRes Reference guide

Roche Cedex HiRes Reference guide

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