Roche Cedex HiRes Reference guide

Cedex HiRes
Roche
Brand
Roche
Model
Cedex HiRes
Type
Reference guide

Roche Cedex HiRes is a sophisticated analyzer designed for precise and efficient cell counting and analysis in various research and clinical applications. With its advanced technology, the device offers a wide range of capabilities:

  • Automated cell counting: Cedex HiRes automates the cell counting process, providing accurate and consistent results for various cell types and sizes.

  • Cell differentiation: The system can differentiate between different cell types based on their size and morphology, enabling researchers to study specific cell populations.

  • Viability assessment: Cedex HiRes evaluates cell viability by distinguishing live and dead cells, aiding in cell culture monitoring and cytotoxicity assays.

Roche Cedex HiRes is a sophisticated analyzer designed for precise and efficient cell counting and analysis in various research and clinical applications. With its advanced technology, the device offers a wide range of capabilities:

  • Automated cell counting: Cedex HiRes automates the cell counting process, providing accurate and consistent results for various cell types and sizes.

  • Cell differentiation: The system can differentiate between different cell types based on their size and morphology, enabling researchers to study specific cell populations.

  • Viability assessment: Cedex HiRes evaluates cell viability by distinguishing live and dead cells, aiding in cell culture monitoring and cytotoxicity assays.

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For use in quality control/manufacturing processes only.
Preparing and Maintaining the Analyzer — (April 2014)
Moving parts of the Multi Sampler may cause hand injuries. The Multi Sampler needle
may cause injuries during operation. Never use the Cedex HiRes Analyzer without the
protective cover in place for the Multi Sampler.
Changing the Reagent Kit
Proceed as follows to install or change the Reagent Kit:
1
If the Liquid Management system has been primed, click on the HWM button in Cedex
Control
Center to open the Hardware Management dialog box.
2
Attach a clean Cedex Sample Cup to the sample port or place a sample cup in the
DefaultCup position on the Multi Sample Tray, and click on LM SHUTDOWN in the
Hardware Management dialog box to shut down the Liquid
Management.
3
Open the reagent chamber in Cedex HiRes Analyzer by pushing the front upwards until it
clicks into place.
4
Remove the old Reagent Kit from the tray and dispose of the old reagent containers if necessary.
5
Open the new Reagent Kit and remove the lids from the individual containers.
6
Place the new Reagent Kit containers in the appropriate positions in the Reagent Tray. The
positions for the reagents are marked on the Reagent Tray.
7
Close the front cover by pulling it all the way down. When the front cover is closed, the
capillaries are automatically correctly positioned in the individual containers.
8
Select the Reagent Kit Status option in the Functions menu of the Cedex Control Center
to open the Reagent Kit Status dialog box.
9
Click on the REPLACE ALL CONTAINERS button in right-hand corner of the Reagent Kit
Status
dialog box to confirm the changing of the Reagent Kit. The Reagent Control display at
the bottom
right-hand corner of the Cedex Control Center will turn from red to green and
indicate the number of runs left.
10
Attach a clean Cedex Sample Cup to the sample port or place a sample cup in the DefaultCup
position
on the Multi Sample Tray, and perform a Prime via the Hardware Management
dialog box
(HWM button on the Cedex Control Center).
■
Refilling Individual Containers
In order to ensure the optimal functioning of the Analyzer, the Reagent Kit containers must
be thoroughly cleaned before refilling with fresh reagents. Reagents can be refilled, or in the
case of waste emptied, individually.
1
Shut down the liquid management via the Hardware Management dialog box as in step
1 — 2 of “Changing the Reagent Kit” above.
2
Open the reagent chamber as described in step 3 of “Changing the Reagent Kit” above.
3
Remove the reagent containers to be refilled, empty them and rinse thoroughly with distilled
water. Empty waste container, if necessary.
4
Add fresh reagents to the appropriate reagent containers and place them back in their
correct positions.
5
Close the reagent chamber.
6
Select the Reagent Kit Status option in the Functions menu of the Cedex Control Center
to open the Reagent Kit Status dialog box.
7
Confirm the refilling of each reagent container individually by clicking on the respective SET
FULL button (or EMPTY button in the case of waste) in the Reagent Kit Status dialog box.
■
Cedex HiRes Software 2.3.2 Quick Guide
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07267096001
3
042014
For use in quality control/manufacturing processes only.
CEDEX is a trademark of Roche.
All other product names and trademarks are the property of their respective owners.
Preparing and Maintaining the Analyzer — (April 2014)
Prescan failure
If the software determines that there are impurities in the flow chamber, an error message,
“Dirt or bubbles detected during prescan!” will appear. When the measurement is viewed in
the Measurement dialog box there will be no check in the Valid checkbox below the Image
area. Do the following:
1
Click on the VIEW PRESCAN button in the Measurement dialog box, a part of the prescan
image
will appear in the image dialog box.
2
Double-click on the partial prescan image to open the complete image in an image view
dialog box
.
3
If the flow chamber appears to have had dirt or impurities in it, clean the system with
Standard Clean or Chamber Clean 2 before performing a new measurement. To carry
out a cleaning routine, click on the CLEAN button in the Cedex Control Center to open
the Cleaning dialog box. Click on Standard Clean and/or Chamber Clean 2.
■
The Cedex Sample Cup from the last measurement should remain attached to the sample
port or placed in the DefaultCup position on the Multi Sample Tray during the complete
cleaning procedure. It should only be removed when a new Cedex Sample Cup has been
placed in position for the next measurement.
Interrupting a Measurement
An orange EMERGENCY STOP button is located on the left-hand side of the Measurement
dialog box and can be activated during the measuring procedure to interrupt the analysis
process. If the EMERGENCY STOP is selected, an error message will appear asking, “Do you
want to cancel the process?”.
c Click on YES to stop the system immediately. If YES is selected, the EMERGENCY STOP
button will change to read Unlock Queue. The system must be unlocked by clicking on
this button before more measurements can be run.
c Click on NO to put the process on hold until another action is chosen. If NO is selected,
the EMERGENCY STOP button will change to read Resume. Click on this button to
resume the measurement process.
Only use the EMERGENGY STOP button in an emergency. Actual measurement data
may be lost.
Published by:
Roche Diagnostics GmbH
Sandhofer Strasse 16
68305 Mannheim
Germany
Cedex HiRes Software 2.3.2 Quick Guide
  • Page 1 1
  • Page 2 2

Roche Cedex HiRes Reference guide

Cedex HiRes
Roche
Brand
Roche
Model
Cedex HiRes
Type
Reference guide

Roche Cedex HiRes is a sophisticated analyzer designed for precise and efficient cell counting and analysis in various research and clinical applications. With its advanced technology, the device offers a wide range of capabilities:

  • Automated cell counting: Cedex HiRes automates the cell counting process, providing accurate and consistent results for various cell types and sizes.

  • Cell differentiation: The system can differentiate between different cell types based on their size and morphology, enabling researchers to study specific cell populations.

  • Viability assessment: Cedex HiRes evaluates cell viability by distinguishing live and dead cells, aiding in cell culture monitoring and cytotoxicity assays.

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