Roche Cedex HiRes Reference guide

Brand: Roche

Model: Cedex HiRes

Type: Reference guide

Roche Cedex HiRes is a state-of-the-art analyzer designed to provide accurate and reliable results for your cell culture analysis needs. With its ability to perform a wide range of assays, including cell counting and viability determination, this device offers a comprehensive solution for cell culture monitoring and quality control. The Cedex HiRes features an intuitive user interface, allowing for easy setup and operation. Its compact design and small footprint make it a great fit for any laboratory setting.

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For use in quality control/manufacturing process only.

Preparing and Maintaining the Analyzer — (October 2017)

Moving parts of the Multi Sampler may cause hand injuries. The Multi Sampler needle

may cause injuries during operation. Never use the Cedex HiRes Analyzer without the

protective cover in place for the Multi Sampler.

Changing the Reagent Kit

To install or change the Reagent Kit:

If the Liquid Management system has been primed, click on the HWM button in Cedex

Control

Center to open the Hardware Management dialog box.

Attach a clean Cedex Sample Cup to the single sample port or place a sample cup in

the DefaultCup position on the Multi Sample Tray, and click on LM SHUTDOWN in the

Hardware Management dialog box to shut down the Liquid

Management.

Open the reagent chamber in the Cedex HiRes Analyzer by pushing the front upwards

until it

clicks into place.

Remove the old Reagent Kit from the tray and dispose of the old reagent containers.

Open the new Reagent Kit and remove the lids from the individual containers.

Place the new Reagent Kit containers in the appropriate positions in the Reagent Tray. The

positions for the reagents are marked on the Reagent Tray.

Close the front cover by pulling it all the way down. When the front cover is closed, the

capillaries are automatically correctly positioned in the individual containers.

Select the Reagent Kit Status option in the Functions menu of the Cedex Control Center

to open the Reagent Kit Status dialog box.

Click on the REPLACE ALL CONTAINERS button in the right-hand corner of the Reagent Kit

Status

dialog box to conﬁrm the changing of the Reagent Kit. The Reagent Control display at

the bottom

right-hand corner of the Cedex Control Center will turn from red to green and

indicate the number of runs left.

Attach a clean Cedex Sample Cup to the single sample port or place a sample cup in the

DefaultCup position

on the Multi Sample Tray, and perform a Prime via the Hardware

Management dialog box

(HWM button on the Cedex Control Center).

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Reﬁlling Individual Containers

In order to ensure the optimal functioning of the Analyzer, the Reagent Kit containers must

be thoroughly cleaned before reﬁlling with fresh reagents. Reagents can be reﬁlled, or in the

case of waste, emptied individually.

Shut down the liquid management via the Hardware Management dialog box as in Steps

1 to 2 of “Changing the Reagent Kit”.

Open the reagent chamber as described in Step 3 of “Changing the Reagent Kit ”.

Remove the reagent containers to be reﬁlled, empty them, and rinse thoroughly with distilled

water. Empty waste container, if necessary.

Add fresh reagents to the appropriate reagent containers and place them back in their

correct positions.

Close the reagent chamber.

Select the Reagent Kit Status option in the Functions menu of the Cedex Control Center

to open the Reagent Kit Status dialog box.

Conﬁrm the reﬁlling of each reagent container individually by clicking on the respective SET

FULL button (or EMPTY button for waste) in the Reagent Kit Status dialog box.

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Cedex HiRes Software 2.4 Quick Guide

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08385807001

102017

For use in quality control/manufacturing process only.

CEDEX is a trademark of Roche.

All other product names and trademarks are the property of their respective owners.

Preparing and Maintaining the Analyzer — (October 2017)

Prescan Failure

If the software determines that there are impurities in the ﬂow chamber, a message, “Dirt

or bubbles detected during prescan!” will appear. When the measurement is viewed in the

Measurement dialog box, there will be no check in the Valid checkbox below the Image area.

Do the following:

Click on the VIEW PRESCAN button in the Measurement dialog box, a part of the prescan

image

will appear in the Image dialog box.

Double-click on the partial prescan image to open the complete image in an image view

dialog box

If the ﬂow chamber appears to have had dirt or impurities in it, clean the system with

Standard Clean or Chamber Clean 2 before performing a new measurement. To perform

a cleaning routine, click on the CLEAN button in the Cedex Control Center to open the

Cleaning dialog box. Click on Standard Clean and/or Chamber Clean 2.

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The Cedex Sample Cup from the last measurement should remain attached to the single

sample port or placed in the DefaultCup position on the Multi Sample Tray during the

complete cleaning procedure. It should only be removed when a new Cedex Sample Cup has

been placed in position for the next measurement.

Interrupting a Measurement

An orange EMERGENCY STOP button is located on the left-hand side of the Measurement

dialog box and can be activated during the measuring procedure to interrupt the analysis

process. If the EMERGENCY STOP is selected, a message will appear asking, “Do you want to

cancel the process?”.

c Click on YES to stop the system immediately. If YES is selected, the EMERGENCY STOP

button will change to read Unlock Queue. The system must be unlocked by clicking on

this button before more measurements can be run.

c Click on NO to put the process on hold until another action is chosen. If NO is selected,

the EMERGENCY STOP button will change to read Resume. Click on this button to

resume the measurement process.

Use the EMERGENGY STOP button only when necessary as measurement data may be

lost.

Cedex HiRes Software 2.4 Quick Guide

Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

Roche Cedex HiRes Reference guide

Roche Cedex HiRes Reference guide

Roche Cedex HiRes Reference guide

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