Bayer HealthCare Advia 70 User manual

Part No. 083D0001-01

ADVIA 70 Hematology System

Operator's Guide

A

Trademarks and Copyright

ADVIA, is a registered trademark of Bayer Corporation. TESTpoint and SETpoint are

trademarks of Bayer Corporation.

Microsoft, Windows, and Windows 2000 are registered trademarks of Microsoft

Corporation.

All other trademarks and service marks used in this document are the property of their

respective owners.

No part of this manual or the products it describes may be reproduced by any means or in

any form without prior consent in writing from Bayer Corporation.

WARNING

If the ADVIA 70 Hematology System is used in a manner not specified by Bayer

Corporation, the protection provided by the equipment may be impaired. See Appendix B

for warning and hazard statements.

CONTENTS i

CONTENTS

CONTENTS .............................................................................................................i

FIGURES..............................................................................................................vii

SECTION 1 — INTRODUCTION.........................................................................1-1

Intended Use ............................................................................................................1-2

Reagents, Calibrators, and Controls.........................................................................1-3

General..............................................................................................................1-3

Damaged Containers.........................................................................................1-4

ADVIA 70 Diluent...............................................................................................1-4

ADVIA 70 Lyse...................................................................................................1-4

ADVIA 70 KLEEN ..............................................................................................1-4

ADVIA 70 Sheath...............................................................................................1-4

ADVIA 70 TESTpoint Controls and SETpoint Calibrators..................................1-5

Laboratory Precautions .....................................................................................1-5

Supplementary Data.................................................................................................1-5

Service Assistance....................................................................................................1-6

This Manual and Training .........................................................................................1-6

SECTION 2 — SETTING UP THE SYSTEM .......................................................2-1

Unpacking the System..............................................................................................2-2

Setting up the Autosampler (optional)................................................................2-3

Placement of the ADVIA 70 Hematology System .....................................................2-5

General Environment.........................................................................................2-5

Power Requirements.........................................................................................2-5

Space Requirements.........................................................................................2-6

Setting up the ADVIA 70 Hematology System ..........................................................2-6

ADVIA 70 Reagents...........................................................................................2-6

Supply Tubing Assemblies................................................................................. 2-7

ADVIA 70 KLEEN ..............................................................................................2-7

ADVIA 70 Diluent...............................................................................................2-7

ADVIA 70 Sheath...............................................................................................2-8

ii CONTENTS

ADVIA 70 Lyse...................................................................................................2-8

Waste ................................................................................................................2-8

Computer...........................................................................................................2-8

Computer Monitor..............................................................................................2-9

Printer (optional)................................................................................................ 2-9

Keyboard ...........................................................................................................2-9

Pointing Device..................................................................................................2-9

Starting the System ................................................................................................ 2-10

Software Configuration ...........................................................................................2-10

Auxiliary Menu........................................................................................................ 2-11

Patient Ranges and Action Limits....................................................................2-13

Autorecount Feature........................................................................................2-15

Printer (optional).............................................................................................. 2-16

Transfer Settings .............................................................................................2-17

Date/Time and Regional Settings .................................................................... 2-18

Customize Report............................................................................................2-20

Wake Up and Shutdown..................................................................................2-23

Advanced Setup ..............................................................................................2-24

Service ............................................................................................................2-24

SECTION 3 — SYSTEM PRINCIPLES ...............................................................3-1

General System Design............................................................................................3-2

Sequence of Operation.............................................................................................3-2

Dilution Phase ...................................................................................................3-2

Counting Phase................................................................................................. 3-3

Rinse Phase ......................................................................................................3-4

Elements of Impedance Cell Counting .....................................................................3-5

Cell Counting and Sizing (RBC, Plt) .........................................................................3-7

Cell Counting (WBC) ..............................................................................................3-13

White Cell Differential .............................................................................................3-13

Hemoglobin Determination (Hgb) ...........................................................................3-17

Mean Cell Volume Determination (MCV)................................................................3-18

CONTENTS iii

Hematocrit Determination (Hct) ..............................................................................3-18

Mean Cell Hemoglobin Determination (MCH).........................................................3-18

Mean Cell Hemoglobin Concentration Determination (MCHC)............................... 3-18

Red Cell Distribution Width (RDW).........................................................................3-19

Mean Platelet Volume Determination (MPV) ..........................................................3-19

Plateletcrit Determination (Pct)...............................................................................3-19

Platelet Distribution Width (PDW)...........................................................................3-19

Clog Detection ........................................................................................................3-20

Patient Action Limits and Normal Ranges...............................................................3-20

SECTION 4 — DAILY OPERATING PROCEDURES ..........................................4-1

Supplies and Accessories.........................................................................................4-2

Specimen Collection and Handling...........................................................................4-2

Startup ......................................................................................................................4-3

System Checks..................................................................................................4-3

Dry Startup ........................................................................................................4-3

Normal Startup ..................................................................................................4-4

Performing the Daily Quality Control Check .............................................................4-5

Open-Tube Sampling................................................................................................4-7

Running Samples in Sample Saver and Direct Patient Modes

without a Worklist ..............................................................................................4-7

Running Samples in Direct Patient Mode with a Worklist................................4-10

Running Stat Samples with a Worklist.............................................................4-12

Closed-Tube Sampling ...........................................................................................4-12

Using the Autosampler with a Worklist: No Barcode Reader...........................4-12

Using the Autosampler with a Worklist: Barcode Reader ................................4-14

Using the Autosampler without a Worklist (Barcode Reader Required)...........4-14

Running a Stat Sample in Autosampler Mode.................................................4-15

Shutdown................................................................................................................4-15

Shutdown without Wake Up............................................................................. 4-16

Shutdown with Wake Up.................................................................................. 4-16

iv CONTENTS

SECTION 5 — DATA ARCHIVE ..........................................................................5-1

File System Description............................................................................................5-1

Data Archive ......................................................................................................5-1

Maintenance Log...............................................................................................5-2

System Log........................................................................................................5-2

SECTION 6 — DATALOG ...................................................................................6-1

General.....................................................................................................................6-2

Opening the Datalog................................................................................................. 6-2

Searching the Datalog ..............................................................................................6-3

Searching by Sequence Number....................................................................... 6-4

Searching by Patient ID Number .......................................................................6-5

Searching by Patient Name...............................................................................6-6

Displaying Results .................................................................................................... 6-7

Changing the Patient ID............................................................................................6-8

Transferring Data......................................................................................................6-9

Printing Data.............................................................................................................6-9

SECTION 7 — CALIBRATION PROCEDURES ..................................................7-1

General.....................................................................................................................7-1

When to Calibrate.....................................................................................................7-1

How to Calibrate the System ....................................................................................7-2

SECTION 8 — QUALITY CONTROL ..................................................................8-1

General.....................................................................................................................8-2

Quality Control System Organization........................................................................8-3

Selecting the Active Control Lot................................................................................8-4

Adding Controls to the System .................................................................................8-5

Setting Up a Quality Control File Using a Disk ..................................................8-5

Setting Up a Quality Control File Manually........................................................8-6

Deleting a Control Lot...............................................................................................8-7

Editing a Control File ................................................................................................8-7

Reviewing Quality Control Results ...........................................................................8-8

Levy-Jennings Charts........................................................................................8-8

CONTENTS v

Quality Control Results....................................................................................8-10

Submitting Control Data by Disk......................................................................8-12



X

B

Analysis ...........................................................................................................8-12

Establishing



X

B

Target Values........................................................................ 8-14

Activating



X

B

Analysis....................................................................................8-14



X

B

Alert ..........................................................................................................8-14

Viewing or Printing



X

B

Graphics ....................................................................8-15

Reviewing Sample Results..............................................................................8-15

Reviewing Batches..........................................................................................8-15

SECTION 9 — MAINTENANCE..........................................................................9-1

General.....................................................................................................................9-2

Maintenance Log......................................................................................................9-3

Adding an Event to the Maintenance Log..........................................................9-3

Adding or Editing a Comment in the Maintenance Log .....................................9-4

Printing the Maintenance Log............................................................................ 9-4

Reagents ..................................................................................................................9-5

Changing Reagents...........................................................................................9-5

Priming Reagent Lines ...................................................................................... 9-7

Monthly or Pre-Calibration Cleaning.........................................................................9-7

Cleaning the Slide Valve....................................................................................9-7

Cleaning the WBC Aperture (No Disassembly) ................................................. 9-9

Semi-Annual Maintenance......................................................................................9-10

Cleaning the Apertures (Disassembly Required)............................................9-10

Annual Maintenance............................................................................................... 9-11

Additional Options................................................................................................... 9-11

SECTION 10 – TROUBLESHOOTING..............................................................10-1

General...................................................................................................................10-2

Overview of Troubleshooting ..................................................................................10-2

Alert or Symptom....................................................................................................10-2

Functional Alerts..............................................................................................10-2

System Message Alerts...................................................................................10-3

vi CONTENTS

Hematology Flags............................................................................................10-7

APPENDIX A — SYSTEM SPECIFICATIONS ................................................... A-1

Operating Characteristics .........................................................................................A-2

Parameters Reported ........................................................................................A-2

Scientific Principles............................................................................................A-3

Sampling Features....................................................................................................A-3

Volume Required for Each Analysis...................................................................A-3

Dilution Ratios ...................................................................................................A-3

Cell Counting Aperture Size ..............................................................................A-3

Cycle Time.........................................................................................................A-3

Waste Analysis ..................................................................................................A-3

Performance Specifications......................................................................................A-4

Computer Hardware .................................................................................................A-5

Processor and Memory .....................................................................................A-5

Display...............................................................................................................A-5

Input/Output Support .........................................................................................A-5

Printer (optional equipment) ..............................................................................A-5

Software Characteristics...........................................................................................A-6

Operating Systems............................................................................................A-6

Software Features .............................................................................................A-6

Alerts .................................................................................................................A-7

Physical Characteristics............................................................................................A-9

Reagents and Controls Required ......................................................................A-9

Power Requirements.........................................................................................A-9

Operating Environment......................................................................................A-9

Dimensions........................................................................................................A-9

Weight ...............................................................................................................A-9

Regulatory Compliance ..........................................................................................A-10

FIGURES vii

APPENDIX B — OPERATIONAL HAZARDS, PRECAUTIONS, AND

LIMITATIONS...................................................................................................... B-1

General.....................................................................................................................B-2

Documentation .........................................................................................................B-3

Electrical Hazards.....................................................................................................B-3

Instrument Precautions.............................................................................................B-3

Reagent Precautions ................................................................................................B-3

Maintenance Precautions .........................................................................................B-4

Calibration Precautions.............................................................................................B-4

Procedural Precautions and Limitations ...................................................................B-5

Results Limitations....................................................................................................B-5

Limitations of the Hct Determination..................................................................B-6

Limitations of the Platelet Count........................................................................B-7

Interpretation of Results............................................................................................B-7

APPENDIX C — BIBLIOGRAPHY..................................................................... C-1

APPENDIX D — ABBREVIATIONS ................................................................... C-1

FIGURES

SECTION 2 — SETTING UP THE SYSTEM

Figure 2-1 Rear Panel View..................................................................................... 2-2

Figure 2-2 Removing the Slide Valve.......................................................................2-3

Figure 2-3 Left Side of the Autosampler .................................................................. 2-4

Figure 2-4 Premix Cuvette.......................................................................................2-4

Figure 2-5 UPS Receptacles ...................................................................................2-5

Figure 2-6 Main Menu............................................................................................2-10

Figure 2-7 Auxiliary menu...................................................................................... 2-11

Figure 2-8 Patient Range - Action Limits Window..................................................2-13

Figure 2-9 Set/Review Patient Range ...................................................................2-14

Figure 2-10 Set/Review Action Limits .................................................................... 2-15

viii FIGURES

Figure 2-11 Printers Window..................................................................................2-16

Figure 2-12 Transfer Settings Box.........................................................................2-17

Figure 2-13 Date and Time Properties...................................................................2-18

Figure 2-14 Regional Settings ...............................................................................2-19

Figure 2-15 Laboratory Information Window..........................................................2-20

Figure 2-16 Misc Information Window ...................................................................2-21

Figure 2-17 Customer Report Setup Dialog Box ...................................................2-22

Figure 2-18 Wake Up / Shutdown Dialog Box........................................................2-23

SECTION 3 — SYSTEM PRINCIPLES

Figure 3-1 Dilution Schematic Diagram...................................................................3-3

Figure 3-2 Impedance Counting System RBC/Plt....................................................3-6

Figure 3-3 RBC/Plt Counting System – Counting in Process ..................................3-8

Figure 3-4 RBC/Plt Counting System – Counting Complete....................................3-9

Figure 3-5 RBC/PLT Counting System – Rinsing Silencer ....................................3-10

Figure 3-6 RBC/Plt Counting System – Backflush Aperture .................................. 3-11

Figure 3-7 RBC/Plt Counting System – Cycle Complete .......................................3-12

Figure 3-8 WBC Differential – Flow Cytometer Scheme........................................3-14

Figure 3-9 Flow Cytometer – Hydrodynamic Focused Cell Monofile.....................3-15

Figure 3-10 WOC Sample Dilution – Injection System ...........................................3-16

Figure 3-11 WOC Scattergrams.............................................................................3-17

SECTION 4 — DAILY OPERATING PROCEDURES

Figure 4-1 Main Menu..............................................................................................4-3

Figure 4-2 Startup Shutdown Window .....................................................................4-4

Figure 4-3 Run Menu...............................................................................................4-7

Figure 4-4 Results Window......................................................................................4-9

Figure 4-5 Worklist Menu.........................................................................................4-9

Figure 4-6 Edit Worklist Window............................................................................4-10

Figure 4-7 Add Patient Window ............................................................................. 4-11

SECTION 6 — DATALOG

Figure 6-1 Datalog Menu .........................................................................................6-2

Figure 6-2 Datalog Search Window.........................................................................6-3

FIGURES ix

Figure 6-3 Search by Sequence Number ................................................................6-4

Figure 6-4 Search by Patient ID ..............................................................................6-5

Figure 6-5 Search by Patient Name .........................................................................6-6

Figure 6-6 Results window ......................................................................................6-7

Figure 6-7 Changing Patient ID ...............................................................................6-8

Figure 6-8 Datalog Print Window.............................................................................6-9

SECTION 7 — CALIBRATION PROCEDURES

Figure 7-1 Calibration Menu ....................................................................................7-2

Figure 7-2 Calibration Run.......................................................................................7-3

Figure 7-3 Calibration Summary Window ................................................................7-4

SECTION 8 — QUALITY CONTROL

Figure 8-1 Quality Control Menu..............................................................................8-3

Figure 8-2 Control Menu..........................................................................................8-4

Figure 8-3 Add Control Window...............................................................................8-5

Figure 8-4 Add Control – Manual Window ...............................................................8-6

Figure 8-5 Add Control – Low Window .................................................................... 8-7

Figure 8-6 Edit Control – Low Window ....................................................................8-8

Figure 8-7 Levy-Jennings Window ..........................................................................8-9

Figure 8-8 Levy-Jennings Chart ............................................................................8-10

Figure 8-9 Control Data Window ........................................................................... 8-11

Figure 8-10 Data Files Window ............................................................................. 8-11

Figure 8-11



X

B

Menu.............................................................................................8-13

Figure 8-12



X

B

Target Window..............................................................................8-13

Figure 8-13



X

B

Chart Window ...............................................................................8-15

Figure 8-14



X

B

Review Samples Window ............................................................. 8-16

Figure 8-15



X

B

Review Batch Window..................................................................8-16

SECTION 9 — MAINTENANCE

Figure 9-1 Maintenance Menu..................................................................................9-3

Figure 9-2 Maintenance Log....................................................................................9-4

Figure 9-3 Replace Reagents Window....................................................................9-5

Figure 9-4 Change Reagents Window.....................................................................9-6

x FIGURES

Figure 9-5 Prime Reagents Window........................................................................9-6

Figure 9-6 Preventive Maintenance.........................................................................9-8

Figure 9-7 Cleaning the Apertures.........................................................................9-10

SECTION 10 — TROUBLESHOOTING

Figure 10-1 Normal FS-WA ...................................................................................10-8

Figure 10-2 Normal EX-SWA.................................................................................10-8

Figure 10-3 N Flag Scattergram ............................................................................10-8

Figure 10-4 B Flag Scattergram ............................................................................10-9

Figure 10-5 B Flag Scattergram ............................................................................10-9

Figure 10-6 L Flag Scattergram........................................................................... 10-10

Figure 10-7 L Flag Scattergram........................................................................... 10-10

SECTION 1 — INTRODUCTION 1-1

SECTION 1 — INTRODUCTION

Intended Use .........................................................................................................2

Reagents, Calibrators, and Controls...................................................................3

General........................................................................................................................ 3

Damaged Containers................................................................................................... 4

ADVIA 70 Diluent......................................................................................................... 4

ADVIA 70 Lyse............................................................................................................. 4

ADVIA 70 KLEEN ........................................................................................................ 4

ADVIA 70 Sheath......................................................................................................... 4

ADVIA 70 TESTpoint Controls and SETpoint Calibrators............................................ 5

Laboratory Precautions................................................................................................ 5

Supplementary Data .............................................................................................5

Service Assistance ...............................................................................................6

This Manual and Training.....................................................................................6

1-2 SECTION 1 — INTRODUCTION

Intended Use

The ADVIA

®

70 Hematology System is intended for the in vitro quantitative

determination of the following parameters:

• white blood cell count (WBC) with a 5-part differential grouped as:

neutrophils (Neut)

lymphocytes (Lymp)

monocytes (Mono)

eosinophils (Eos)

basophils (Bas)

• red blood cell count (RBC)

• hemoglobin (Hgb)

• hematocrit (Hct)

• mean corpuscular volume (MCV)

• mean corpuscular (cell) hemoglobin (MCH)

• mean corpuscular hemoglobin concentration (MCHC)

• red cell distribution width (RDW)

• platelet count (Plt)

• mean platelet volume (MPV)

• platelet crit (Pct)*

• platelet distribution width (PDW)*

* In the U.S.A., for research use only

The abbreviations in parenthesis are those used by the instrument to represent the

respective parameters.

Triple, closed systems automatically dilute, mix and analyze white cell, red cell,

platelet, and hemoglobin related parameters separately and simultaneously.

The WBC system makes two dilutions, one for counting white cells and the other

for the optical scattering and absorption analysis. The WBC total count is

measured in the first dilution and the distribution of cell types is determined from

the second. The differential results include the calculated absolute numbers and

percent values for the groups: neutrophils, lymphocytes, monocytes, eosinophils,

and basophils.

SECTION 1 — INTRODUCTION 1-3

Two WBC-attribute distribution scattergrams are provided on screen and in each

printed report. One scattergram displays the Forward Scattering (FS) vs. Wide-

Angle (WA) scattering. This presentation shows the lymphocytes, monocytes,

basophils, and neutrophils as four groups or clusters of cells. The second

scattergram displays the Super Wide-Angle scattering (SWA) vs. on-axis

Extinction (EX). This view of the optical data identifies the eosinophils.

The WBC impedance cuvette (WIC) is equipped with an optical absorbency

measuring system for determining the hemoglobin concentration using the

cyanmethemoglobin method.

The RBC system counts and groups red cells and platelets by size to provide size

distribution histograms for each on the printed report. Additional red cell and

platelet size information (MCV, Hct, RDW, MPV, PDW, and Pct) is calculated

and presented in tabular form both on the system and in the printed report. (Note:

In the U.S.A., PDW and Pct are for research use only.)

The system displays results on the monitor, in the Patient Report window of the

software. The system can also print results with a printer attached to the external

computer. If the laboratory is so equipped, results can also be transferred to a host

computer or laboratory information system (LIS).

In the Direct Mode, the system aspirates, automatically dilutes, and analyzes

EDTA-treated, whole-blood samples.

In the Sample Saver Mode, the system performs the analysis using only 80µL of

sample.

In the (optional) Autosampler Mode, the system sequences the operation of the

thirty tube ADVIA 70 autosampler, mixes the blood samples, pierces the tube cap

(septum), aspirates the needed amount of whole blood, automatically dilutes the

sample, and performs the analysis.

For a description of the scientific concepts used for determining parameters, see

Section 3 - Principles of the System.

Reagents, Calibrators, and Controls

General

Because the analyzer, reagents, controls, and calibrators are components of a

system, performance of the system depends on the combined integrity of all

components. The user must ensure that the products used with this instrument

have been tested and are certified by the manufacturer for use with the ADVIA

70 Hematology System. If in doubt, contact your technical service representative.

1-4 SECTION 1 — INTRODUCTION

The ADVIA 70 Hematology System is designed to be used with Bayer reagents.

The system uses individual packages of diluent, lyse, sheath, and cleaner reagent.

Each package contains enough reagent for about 500 tests.

Do not use the system with reagents and/or controls from multiple suppliers. In

such use, the system may not meet the performance specified in this manual and

may provide unreliable results. All references related to reagents in this manual

refer to Bayer reagents specifically formulated for this instrument.

Damaged Containers

Each reagent container must be examined before use. Inspect the package for

signs of leakage or moisture. Product integrity may be compromised in packages

that have been damaged. If there is evidence of leakage or improper handling, do

not use the reagent.

ADVIA 70 Diluent

ADVIA 70 Diluent is an azide-free, isotonic liquid for diluting whole blood. This

diluent is formulated to produce a total white blood cell count and a hemoglobin

measurement when used with ADVIA 70 Lyse.

ADVIA 70 Lyse

ADVIA 70 Lyse is an azide-free lysing reagent that rapidly breaks down red

blood cell walls, releases the hemoglobin from the cell, and reduces the size of

cellular debris to a level that does not interfere with white blood cell counting.

The reagent also contains a cyanide radical that combines with the free

hemoglobin to form cyanmethemoglobin, the absorption of which is directly

proportional to the hemoglobin concentration over the clinically useful range.

ADVIA 70 Lyse must be used with ADVIA 70 Diluent. This reagent is stable for

30 days after opening.

ADVIA 70 KLEEN

ADVIA 70 KLEEN is an enzyme-based, azide-free, isotonic, cleaning solution

and wetting agent. It is used in metering tubes during the counting cycle and

provides a rinse solution for apertures at the completion of the cycle. During the

Shutdown Cycle, the apertures, flow cell, and cuvettes are cleaned and then

remain bathed in this reagent until the next Startup Cycle.

ADVIA 70 Sheath

ADVIA 70 Sheath serves two purposes in the ADVIA 70 Hematology System:

SECTION 1 — INTRODUCTION 1-5

• To make a dilution of whole blood for injection into the flow stream of the

optical cuvette. This reagent is hypotonic and reduces red cell debris to

particles small enough in size that they do not interfere with light scatter

measurements of the WBCs.

• To provide the focusing sheath flow for the optical cuvette. The sample

dilution is inserted into this sheath flow stream in such a manner that a small

column of cells is located near the center of the flow cell channel.

ADVIA 70 TESTpoint Controls and SETpoint Calibrators

Whole-blood calibrators and controls are used to calibrate and verify accurate

operation of the ADVIA 70 Hematology System.

ADVIA 70 SETpoint Calibrator is a commercially prepared whole-blood product

with assigned values traceable to reference methods. Once they are opened,

calibrators are stable for 5 days.

ADVIA 70 TESTpoint Hematology Controls are commercially prepared whole-

blood products used to verify that the analyzer is functioning properly. They are

available in low, normal, and high levels. Daily use of all levels verifies the

operation of the analyzer over a wide range of values as observed in patient

samples. Once they are opened, calibrators are stable for 7 days.

For meeting specified performance, use only the controls and calibrators certified

for use with the ADVIA 70 Hematology System.

Laboratory Precautions

Exercise normal laboratory precautions required for handling laboratory reagents.

Reagents must be stored at room temperature (18 °C to 30 °C or 60 °F to 90°F)

unless otherwise specified. Reagents must be stabilized at laboratory temperature

for eight hours before use.

Treat controls and calibrators as you would treat any blood sample. Store them in

a refrigerator at the temperature stated on package insert that is supplied with the

product.

Refer to the package labeling or Material Safety Data Sheet (MSDS) for safe

handling procedures.

Supplementary Data

The manufacturer may occasionally issue and deliver supplementary bulletins

pertaining to the instrument. Read them and follow the instructions carefully.

Insert the bulletins into this manual for convenient reference.

1-6 SECTION 1 — INTRODUCTION

Service Assistance

If the ADVIA 70 Hematology System does not operate properly, follow the

directions in Section 10 - Troubleshooting. If the problem cannot be corrected

with the information and procedures presented, contact your distributor or Bayer

technical service for assistance.

When calling, you will be asked to furnish your customer identification number

(normally the instrument serial number). This serial number is available from the

Main Menu of the analyzer. Have this number ready before calling.

During phone calls, service representatives may ask you to open the front of the

instrument and inspect the mechanism. If a minor adjustment is needed, the

representative may invite you to make the adjustment to avoid the delay caused

by a service call or a temporary shutdown of the system. The front cover is

snapped on with spring catches on each side. It can be opened by grasping the

right side and pulling it straight away from the unit.

Close the front cover by pushing it straight back until it snaps into place.

This Manual and Training

IMPORTANT

To ensure the instrument performs as specified, follow manual instructions

explicitly.

This manual contains the necessary instructions for operating the ADVIA 70

Hematology System and obtaining reliable data on patient blood samples. It also

contains the instructions for routine instrument calibration and maintenance that

must be performed to ensure reliable system performance.

Read this manual thoroughly, and then follow the steps in Section 2 to configure

the instrument in the manner that best suits the laboratory. All operators should

study the manual and receive training on sample handling, record keeping, and

the use of this instrument.

The importance of training cannot be overstated. Operators should receive formal

training with this manual used as a guide. Periodic reviews, retraining and

evaluations are strongly recommended.

Though computer technology has removed a great deal of the tedious work and

variability in testing, this instrument’s reliability still depends on the operator’s

attention to cleanliness, instruction details, and accepted laboratory procedures.

SECTION 2 — SETTING UP THE SYSTEM 2-1

SECTION 2 — SETTING UP THE SYSTEM

Unpacking the System .........................................................................................2

Setting up the Autosampler (optional).......................................................................... 3

Placement of the ADVIA 70 Hematology System ...............................................5

General Environment................................................................................................... 5

Power Requirements ................................................................................................... 5

Space Requirements ................................................................................................... 6

Setting up the ADVIA 70 Hematology System ....................................................6

ADVIA 70 Reagents..................................................................................................... 6

Supply Tubing Assemblies........................................................................................... 7

ADVIA 70 KLEEN ........................................................................................................ 7

ADVIA 70 Diluent......................................................................................................... 7

ADVIA 70 Sheath......................................................................................................... 8

ADVIA 70 Lyse............................................................................................................. 8

Waste...........................................................................................................................8

Computer..................................................................................................................... 8

Computer Monitor........................................................................................................ 9

Printer (optional) .......................................................................................................... 9

Keyboard ..................................................................................................................... 9

Pointing Device............................................................................................................ 9

Starting the System ............................................................................................10

Software Configuration ......................................................................................10

Auxiliary Menu .................................................................................................... 11

Patient Ranges and Action Limits.............................................................................. 13

Autorecount Feature.................................................................................................. 15

Printer (optional) ........................................................................................................ 16

Transfer Settings ....................................................................................................... 17

Date/Time and Regional Settings .............................................................................. 18

Customize Report...................................................................................................... 20

Wake Up and Shutdown ............................................................................................ 23

Advanced Setup ........................................................................................................ 24

Service....................................................................................................................... 24

2-2 SECTION 2 — SETTING UP THE SYSTEM

Figure 2-1 Rear Panel View

Unpacking the System

1. Unpack the equipment carefully and examine all items for damage. File a

claim with the shipping carrier immediately if physical damage is discovered.

2. Check the equipment received against the packing list to ensure that the

shipment is complete. If the shipment appears to be incomplete, notify your

distributor immediately.

3. Place the instrument on the bench. Remove the shipping screws from the

bottom of the instrument. Identify the red arrow on the right side of the

instrument. Tilt the instrument to the left and locate the red shipping screw.

Use a screwdriver to remove the screw. Failure to remove this screw may

result in damage to the instrument.

4. Before power is applied to the instrument, remove the front cover of the

analyzer by pulling it straight forward. Remove the shipping screws that are

identified by large red washers. These screws secure the laser optical system

for shipment.

5. With the wash block in the down position, remove the thumb screw from the

lower front of the clear wash block. Carefully lower the wash block off the

sample probe.

Note position of the tubing as well as the shape of the wash block and the

small guide bearing in the top, which centers the sample probe. This will

assist in installing the block after cleaning.

6. Locate the red and white label attached to the slide valve. This label must be

removed before power is applied to the instrument. Remove the front part of

the slide valve by loosening the knurled thumb screw in the center of the

slide valve. Use hand pressure only to remove the screw.

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Bayer HealthCare Advia 70 User manual

Bayer HealthCare Advia 70 User manual

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