8 405-DPAS-SC-VP pH Electrodes
© 04 / 16 Mettler-Toledo GmbH 405-DPAS-SC-VP pH Electrodes
53 500 197 Printed in Switzerland
1 Introduction
TheMETTLERTOLEDOType405-DPAS-SC-VPcombination
pH electrode is a low-maintenance, pre-pressurized,
autoclavable electrode with a gel-type reference and
aVarioPin(VP)connector.Thereferenceelementincorporates
asilver-iontraptopreventsilversuldecontaminationof
thereferencejunction.
2 Preparation
Afterunpacking,examinetheelectrodeforanymechanical
damage.
METTLERTOLEDO electrodes are factory tested and are
shippedready-to-use.(Tomaintainfactorypressurization,
theType405-DPAS-SC-VPelectrodeshaveasiliconeseal
coveringtheceramicdiaphragm;thismustberemovedwith
theenclosedbladepriortouse.)Removethehydrationcap
andremoveanyelectrolytecrystalsbyrinsingtheelectrode
withdistilledwater.Patdry;donotrubasthismaybuildup
astaticchargeandlengthentheresponsetime.
Eliminateanyairbubblesaroundtheinternalelementinthe
pH-sensitive membrane.
3 Operation
METTLERTOLEDO pH electrodes can be used with most
pH/mVmeters.Followmanufacturer’sinstructionsforuse.
Atwo-pointcalibrationisrecommended,usingfreshbuffers
ofknownpHvalueswhichbracketthesamplepH;onebuffer
shouldbeclosetopH7tosetthezeropoint.Forfastroutine
analysis,astandardizationusingonebufferatavaluenear
thesamplepHcanbedone.
4 Sterilization
Type 405-DPAS-SC-VP electrodes are steam-sterilizable
in-situandauto-clavable.Followoperatinginstructionsfor
theautoclave.Itisnotnecessarytouseaprotectivecapon
theconnectorwhenautoclaving.
Repeatedsterilizationmaycausetheelectrolytetodiscolor
slightlyduetotheelevatedtemperatures.Thisdoesnotaffect
theelectrodeperformance.
5 Maintenance
When properly cared for, METTLER TOLEDO electrodes
shouldprovideaccurateresultsoverthousandsofuses.
Storage:Electrodesshouldberinsedthoroughlyafteruse
andstoredin3MKClorpH4buffersolutionwithaddedKCl.
DONOTstoretheelectrodedryorindistilledortapwater.
Iftheelectrodehasbeenstoreddryorinwater,itmustbe
reconditionedbysoakingin3MKClforseveralhours.
Relling: Type 405-DPAS-SC-VP gel-reference electrodes
arenotrellable.
Protein Contamination:CleantheelectrodewithpHElectrode
CleanerforProteinsfollowinginstructionsonthebottle.
Reactivating pH membrane: Use Reactivating Solution
followingdirectionsCAREFULLY.
en
en Instruction manual
Instructions for Type 405-DPAS-SC-VP
pre-pressurized gel-reference pH Electrodes