2. GALENVS
  3. magneti Plant RNA Extraction Kit
magneti Plant RNA Extraction Kit

GALENVS magneti Plant RNA Extraction Kit User guide

  • I have reviewed the magnetiC Plant RNA Extraction Kit Quick Start Guide. This document provides detailed instructions for extracting RNA from plant leaves. It covers the entire process, from sample preparation to final elution, including DNase treatment. I am ready to assist with any questions you may have about this procedure.
  • How much plant material should be used?
    What speed should be used for centrifugation?
    How long should the binding mixture be incubated?
Place tube on magnetic rack for 2 mins.
Discard supernatant.
Resuspend beads in 600µl Wash Buer #3.
Vortex for 10–20s.
Place tube on magnetic rack, wait for 1 min
and discard supernatant.
Wash Buer #3
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Wait 1 min then transfer supernatant to
clean microfuge tube..
11
Add 100µl of Elution Buer to tube and
mix briefly. Wait 1 min.
Place tube on magnetic rack to capture.
Elution Buer
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Add 600µl of Wash Buer #2 to the tube
and vortex for 10–20s.
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #2
7
Add 600µl of Wash Buer #1 to the tube
and vortex for 10–20s.
Wait 1 min then place tube on magnetic
rack to capture.
Wait 1 min then discard supernatant.
Wash Buer #1
6
Place tube on magnetic rack to capture.
Wait 1 min then discard supernatant.
5
2Add 600µl of Lysis Buer and 60µl PPB.
Mix for 3 mins using TissueLyser at max
speed or vortex for 10 mins; then
centrifuge at 20,000g for 2 mins.
20,000g
PPB
Lysis Buer
Add up to 50mg of ground fresh plant
leaves to the lysis bead tube provided.
1Avoiding pellet, transfer up to 300–400µl of
supernatant to clean centrifuge tube.
Add 1000µl of Binding Buer, vortex for
10–20s, and wait 5 mins.
Binding Buer
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Plant RNA Extraction Kit
Quick Start Guide
Elution Buer
Add 50µl of Elution Buer to tube and mix
briefly.
Wash Buer #3
Repeat washing step with Wash Buer #3. Dry beads on magnetic rack for 2 mins
after the third wash.
Remove any wash buer left at the bottom
of tube at the end of drying step.
DNase
For each RNA sample, prepare DNase
buer by adding 10µl of DNase prepared in
the previous step to 40µl of DNase
Reaction Buer in a microfuge tube.
Mix DNase with buer by gently inverting
the tube a few times.
Add 100µl of DNase Reaction Buer to
DNase pellet and then add 100µl of glycerol.
Mix by gently inverting the tube.
Reconstituted pellet must be stored
at -20 °C.
DNase Reaction Buer
Glycerol
DNase Buer
Add 50µl of DNase buer to 50µl of RNA
sample in a microfuge tube.
Gently shake the mix for 20s, and incubate
at room temperature for 25 mins.
Mix 40µl of Binding Beads with 400µl
Binding Buer #2.
Binding Buer #2
Add 400µl of Binding Bead and Binding
Buer #2 mixture to microfuge tube and
vortex for 10s.
Incubate the mixture for 5 mins at room
temperature.
Binding Bead &
Buer Mix
Place tube on magnetic rack, wait for 1 min
then transfer supernatant to clean tube.
Plant RNA Extraction Kit
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