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magneti

GALENVS magneti User guide

  • Hello! I've reviewed the user guide for the GALENVS PCR Clean Up Kit. I understand it details the procedures for using the kit with the miQron device, including information about buffer usage and protocol parameters. I'm ready to assist with any questions you may have about the PCR Clean Up Kit or the steps involved.
  • What is the volume of Binding Buffer used?
    Which columns are used for the wash step?
    What is the volume of Elution Buffer?
Columns 5 and 11 contain the purified
DNA elution.
5Select the PCKit protocol and press
When program is complete, remove
plate from miQron and discard combs.
Run
Report
Settings
Run 05-19-2021 11:16
PCKit
Insert two combs.
4
3Place plate into the miQron, taking care
that the label is facing outward.
2Add up to 16 samples to Binding Buer
(columns 1 and 7).
Remove the protective foil.
1
Binding Buer (150µl)
Columns 1 & 7
Elution Buer (90µl)
Columns 5 & 11
Wash Buer (400µl)
Columns 2 & 8
Columns 3 & 9
90µl extracted
and purified
RNA elution
50µl sample
added to
Binding Buer
90µl extracted
and purified
RNA elution
50µl sample
added to
Binding Buer
Step Name
Lysis / Binding
Wash
Wash
Elution
Discard Comb
Column
1 & 7
2 & 8
3 & 9
5 & 11
2 & 8
Volume
(µl)
200
400
400
90
400
Time
(sec)
300
60
60
60
0
Mixing Speed
(1–10)
5
5
5
5
5
Dry Time
(sec)
0
0
300
0
0
Magnet Capture
Time (sec)
120
120
120
120
0
PCKit miQron protocol parameters
PCR Clean Up Kit  1.0

PCR Clean Up Kit
Quick Start Guide
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