GALENVS magneti User guide

Type
User guide
Columns 5 and 11 contain the purified
DNA elution.
5Select the PCKit protocol and press
When program is complete, remove
plate from miQron and discard combs.
Run
Report
Settings
Run 05-19-2021 11:16
PCKit
Insert two combs.
4
3Place plate into the miQron, taking care
that the label is facing outward.
2Add up to 16 samples to Binding Buer
(columns 1 and 7).
Remove the protective foil.
1
Binding Buer (150µl)
Columns 1 & 7
Elution Buer (90µl)
Columns 5 & 11
Wash Buer (400µl)
Columns 2 & 8
Columns 3 & 9
90µl extracted
and purified
RNA elution
50µl sample
added to
Binding Buer
90µl extracted
and purified
RNA elution
50µl sample
added to
Binding Buer
Step Name
Lysis / Binding
Wash
Wash
Elution
Discard Comb
Column
1 & 7
2 & 8
3 & 9
5 & 11
2 & 8
Volume
(µl)
200
400
400
90
400
Time
(sec)
300
60
60
60
0
Mixing Speed
(1–10)
5
5
5
5
5
Dry Time
(sec)
0
0
300
0
0
Magnet Capture
Time (sec)
120
120
120
120
0
PCKit miQron protocol parameters
PCR Clean Up Kit  1.0

PCR Clean Up Kit
Quick Start Guide
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GALENVS magneti User guide

Type
User guide

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