FLOW PCR Setup Instrument, Operator’s Guide Version 5.0
Table of contents
4
5 Aborting a run ................................................................................................................................................ 86
5.1 Operator-controlled abort ............................................................................................................................ 86
5.2 Instrument-controlled abort ........................................................................................................................ 86
5.3 Emergency shutdown .................................................................................................................................... 86
6 Administrative tasks .................................................................................................................................. 87
6.1 User management ........................................................................................................................................... 87
6.2 Worklists ............................................................................................................................................................. 87
6.2.1 FLOW configuration ........................................................................................................................................ 87
6.2.2 Standalone configuration ............................................................................................................................. 87
6.3 Application Configuration Editor ............................................................................................................... 89
6.4 Order templates for MagNA Pure 96 Software .................................................................................... 92
6.5 Network interaction ........................................................................................................................................ 92
7 FLOW PCR Setup System in PCR Setup mode ............................................................................ 93
7.1 Starting the software ...................................................................................................................................... 93
7.2 The main window ............................................................................................................................................ 93
7.2.1 Information bar ................................................................................................................................................. 94
7.2.2 Working window area .................................................................................................................................... 94
7.2.3 Global action bar ............................................................................................................................................. 94
7.2.4 Status bar ........................................................................................................................................................... 95
7.3 Closing the software ....................................................................................................................................... 95
8 PCR Setup FLOW configuration ........................................................................................................... 96
8.1 Settings ................................................................................................................................................................ 96
8.2 PCR Test Setup Overview ............................................................................................................................. 97
8.2.1 Reaction Mix Preparation - Overview tab .............................................................................................. 97
8.2.2 Transfer Reaction Mixes - Overview tab ................................................................................................. 98
8.2.3 Transfer RT Mixes - Overview tab ............................................................................................................. 99
8.3 PCR Test Setup Execute ................................................................................................................................ 99
8.3.1 Processing view ............................................................................................................................................. 100
8.3.2 Open/Close vertical cover message box .............................................................................................. 101
8.3.3 Load tips message box ............................................................................................................................... 102
8.3.4 Edit Tip Count dialog box .......................................................................................................................... 102
8.3.5 Load Reagents message box ................................................................................................................... 104
8.3.6 Missing Tubes message box .................................................................................................................... 105
8.3.7 Load labware message box ...................................................................................................................... 105
8.3.8 Successfully processed message box ................................................................................................... 106
8.4 PCR Test Setup Review .............................................................................................................................. 106
8.4.1 Reaction Mix Preparation - Review tab ............................................................................................... 106
8.4.2 Transfer Reaction Mixes - Review tab .................................................................................................. 107
8.4.3 Transfer RT Mixes - Review tab .............................................................................................................. 109
8.4.4 Continue button ............................................................................................................................................ 109
8.5 PCR Sample Transfer Overview ............................................................................................................... 110
8.5.1 Transfer Samples - Overview tab ........................................................................................................... 110
8.5.2 Transfer Controls - Overview tab ............................................................................................................ 111
8.6 PCR Sample Transfer Execute ................................................................................................................. 111
8.6.1 Unload of Carriers message box ............................................................................................................ 112
8.6.2 Open/close vertical cover message box .............................................................................................. 112
8.6.3 Load Controls message box ..................................................................................................................... 112
8.6.4 Load labware message box ...................................................................................................................... 112
8.6.5 Load tips message box ............................................................................................................................... 112
8.6.6 Edit Tip Count dialog box .......................................................................................................................... 113
8.6.7 Successfully processed message box ................................................................................................... 113
8.7 PCR Sample Transfer Review ................................................................................................................... 113
8.7.1 Transfer Samples - Review tab ............................................................................................................... 113
8.7.2 Transfer Controls - Review tab ................................................................................................................ 114
8.8 End of run ........................................................................................................................................................ 115
9 Aborting a run ............................................................................................................................................. 116
9.1 Operator-controlled abort ......................................................................................................................... 116
9.2 Instrument-controlled abort ..................................................................................................................... 116
9.3 Emergency shutdown ................................................................................................................................. 116