Roche LightCycler 96 System Reference guide

Brand: Roche

Model: LightCycler 96 System

Type: Reference guide

For life science research only. Not for use in diagnostic procedures.

LightCycler

96 System Quick Guide: Programming and running an experiment

Workflow

To program and run an experiment, use:

 LightCycler® 96 Application Software for defining an experiment protocol and

analyzing acquired data.

 LightCycler® 96 Instrument Software for defining an experiment protocol and

performing experiments.

For detailed step-by-step information, refer to the

LightCycler® 96 System User Training Guide on the LightCycler® 96 USB Drive.

Set up the reaction mix

Define the experiment

Run the experiment

Identify the detection dye to be used in your experiment.

Prepare the PCR mix and set up the sample dilutions.

When setting up the PCR mix, you should compensate for pipetting losses. It is

recommended to prepare PCR mixes with 10% extra volume.



Run

experiment

Define

experiment

Edit

sample list

Analyze

results

Transfer experiment definition

Transfer raw data

Define

experiment

LightCycler

96 Application Software LightCycler

96 Instrument Software

Pipette the PCR mix and the corresponding sample dilution into each well of the

LightCycler

480 Multiwell Plate 96.

Seal the multiwell plate with the LightCycler

480 Sealing Foil using the sealing foil

applicator (part of the system package).

Centrifuge the multiwell plate at 1,500 x g for 2 minutes in a standard swing-bucket

centrifuge, using a rotor for multiwell plates and suitable adapters.



Create a new experiment using the LightCycler

96 Application Software or the

LightCycler

96 Instrument Software.

Open the Run Editor tab and define the temperature profile, including the heating and

cooling cycles to be used.

Configure the detection format and the sample volume.

Save the experiment.



If you have defined the experiment using the LightCycler

96 Application Software,

transfer the experiment file to the LightCycler

96 Instrument.

 If the instrument is connected to an Ethernet network, use the Instrument Manager

in the application software to send the experiment file to the instrument.

 If the instrument is not connected to an Ethernet network, use a USB drive to trans-

fer the experiment file to the instrument. The experiment file has to be saved into a

top level Experiments folder on the USB drive.

Insert the LightCycler

480 Multiwell Plate 96 with the samples into the

LightCycler

96 Instrument.

On the Overview tab on the touchscreen, select the experiment in the list.



For life science research only. Not for use in diagnostic procedures.

LightCycler

96 System Quick Guide: Programming and running an experiment

Run finished

The end of a run is indicated as follows:

 The status bar on the touchscreen displays the instrument status Ready.

 The LightCycler® 96 Instrument unlocks the loading module.

 The experiment progress window area shows the end time of the experiment run.

 The Raw Data tab provides the final raw data.

Transfer the experiment to the application software

Edit the sample list

Analyze the data

Disclaimer

Before setting up operation of the LightCycler® 96 System, it is important to read the user

documentation completely. Non-observance of the instructions provided or performing

any operations not stated in the user documentation could produce safety hazards.

In the global action bar on the right, choose the Start button.

View the Raw Data tab to monitor the progress of the running experiment.



 If the instrument is connected to an Ethernet network, use the Instrument Manager

in the application software to retrieve the experiment file from the instrument.

 If the instrument is not connected to an Ethernet network, use a USB drive to trans-

fer the experiment file to your computer.

Edit the experiment according to your needs and save the file to your computer.



Open the Plate View taboftheSample Editor.

Use the Clear Wells function to clear the empty wells. This eliminates the selected wells

from further analyses.

Select a well or a range of wells.



In the Reaction Properties window area to the right of the multiwell plate image, edit the

sample-specific properties.

Ensure that the sample assignment on the ‘Sample Editor’ tab matches the pipet-

ting scheme on the multiwell plate.

Save the experiment.



On the Analysis tab, add the appropriate analysis type.

Open the <analysis> Settings dialog box and set up the analysis-specific parameters.

Exclude samples if necessary.

Select the results to be displayed.

Optional: Export the result data.



Version Information

Version 2.0, May 2013, Software Version 1.1.

Trademarks

LIGHTCYCLER is a trademark of Roche.

Published by

Roche Diagnostics GmbH

Sandhofer Straße 116

68305 Mannheim

Germany

www.roche-applied-science.com

For patent license limitations for individual products, please refer to: www.technical-support.roche.com.

Roche LightCycler 96 System Reference guide

Roche LightCycler 96 System Reference guide

Roche LightCycler 96 System Reference guide

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