Roche LightCycler 96 System Reference guide
- Type
- Reference guide
Roche LightCycler 96 System
The Roche LightCycler 96 System is a high-performance real-time PCR instrument designed for demanding research applications. It features:
- 96-well format
- Fast and accurate temperature control
- Real-time fluorescence detection
- Intuitive touchscreen interface
With its advanced capabilities, the LightCycler 96 System can be used for a wide range of applications, including:
- Gene expression analysis
- Genotyping
- Microbial detection
- Mutation analysis
The system comes with powerful software that allows for easy experiment setup, data analysis, and reporting.
Roche LightCycler 96 System
The Roche LightCycler 96 System is a high-performance real-time PCR instrument designed for demanding research applications. It features:
- 96-well format
- Fast and accurate temperature control
- Real-time fluorescence detection
- Intuitive touchscreen interface
With its advanced capabilities, the LightCycler 96 System can be used for a wide range of applications, including:
- Gene expression analysis
- Genotyping
- Microbial detection
- Mutation analysis
The system comes with powerful software that allows for easy experiment setup, data analysis, and reporting.
For life science research only. Not for use in diagnostic procedures.
LightCycler
®
96 System Quick Guide: System installation
Unpack the instrument
The LightCycler® 96 Instrument and the accessories are packaged in a shipping box.
Check for damage that may have occurred during transportation. Report any signs of
damage to your local Roche Diagnostics representative.
Keep the shipping box and packaging in case of return.
If you have already disposed of the packaging, you can request it from Roche.
For detailed information on assembling the instrument and more detailed pictures,
refer to the ‘Operator’s Guide’ on the LightCycler® 96 USB Drive.
Assemble the instrument
Number Quantity Component
1 LightCycler
®
96 Instrument
(1) 1 Mains power cable (EU)
(2) 1 Mains power cable (US)
(3) 1 Ethernet cable (3 m)
(4) 1 LightCycler
®
96 USB Drive
(5) 1 Package fuses FUSE 5x20 T8AH 250V ULR/IEC
(6) 2 Ventilation dust filters
(7) 1 Sealing foil applicator
(1) (2) (3) (4) (5) (6) (7)
Remove the protective foam on the top and the accessory box located in front of the
instrument.
Lift the LightCycler
®
96 Instrument out of the box by holding it on the left and right
sides, and place it on a solid level surface.
To carry and lift the instrument, only use the recessed grips on the left and right
sides of the instrument base plate.
Caution: Due to the weight of the instrument, two persons may be needed to lift it.
Ensure that all components are present and intact.
Report any missing items to your local Roche Diagnostics representative.
Remove the protective foil from the instrument, and fully
loosen the fixation gripper on the back of the instrument.
Turn the screw counterclockwise.
Connect the supplied mains power cable to the mains power socket of the instrument,
and then to the wall outlet.
Optional, when connecting the instrument to an Ethernet network:
Connect one end of the Ethernet cable directly to the Ethernet port of your com-
puter or the Ethernet port of your LAN.
Connect the other end of the Ethernet cable to the Ethernet por t on the back of the
LightCycler
®
96 Instrument.
For configuring the instrument using a direct connection or the local Ethernet,
refer to the ‘Operator’s Guide’ on the LightCycler
®
96 USB Drive.
Optional: Connect the external handheld barcode scanner to the USB interface on the
back of the instrument.
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For life science research only. Not for use in diagnostic procedures.
LightCycler
®
96 System Quick Guide: System installation
Remove the transport locking device
Install the LightCycler
®
96 Application Software
For installing the LightCycler® 96 Application Software Version 1.1 as an upgrade,
refer to the ‘Operator’s Guide’ on the LightCycler® 96 USB Drive.
New software releases and user guides for the LightCycler® 96 Instrument are avail-
able in the download area of the Roche Applied Sciences website.
Disclaimer
Before setting up operation of the LightCycler® 96 System, it is important to read the user
documentation completely. Non-observance of the instructions provided or performing
any operations not stated in the user documentation could produce safety hazards.
Switch on the instrument using the mains power switch on the back of the instrument.
The initialization process begins.
When the instrument has successfully initialized, choose the Eject button on the touch-
screen to release the loading module. The loading module is ejected.
Manually pull the loading module completely out of the instrument.
Remove the transport locking device held by an adhesive
tape from the mount.
Keep the transport locking device including the
adhesive tape in case the instrument has to be
transported.
Ensure that no residuals of the tape remain on the
thermal block cycler unit.
Push the loading module in until it starts moving automatically to its home position.
Start the computer on which you want to install the software.
For a detailed list of the system requirements, refer to the ‘Operator’s Guide’ on
the LightCycler
®
96 USB Drive.
Insert the LightCycler
®
96 USB Drive into a USB interface on your computer.
Log on to Microsoft Windows, and ensure that you have the administration rights to
install the software.
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Navigate to the USB drive, and install the software by double-clicking the
Setup_LightCycler96_<release>.exe file.
The installation process transfers files, extracts the files, and prepares the installation
wizard.
For the optional connection of the application software and the instrument software
(usable for online monitoring and data transfer):
Start the LightCycler
®
96 Application Software.
Open the Instrument Manager.
Register the instrument with the application software.
For registering the instrument and monitoring an instrument run via the network,
refer to the ‘Operator’s Guide’ on the LightCycler
®
96 USB Drive.
Version Information
Version 2.0, May 2013, Software Version 1.1.
Trademarks
LIGHTCYCLER is a trademark of Roche.
© 2012 Roche Diagnostics. All rights reserved.
Published by
Roche Diagnostics GmbH
Sandhofer Straße 116
68305 Mannheim
Germany
www.roche-applied-science.com
For patent license limitations for individual products, please refer to: www.technical-support.roche.com.
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Roche LightCycler 96 System Reference guide
- Type
- Reference guide
Roche LightCycler 96 System
The Roche LightCycler 96 System is a high-performance real-time PCR instrument designed for demanding research applications. It features:
- 96-well format
- Fast and accurate temperature control
- Real-time fluorescence detection
- Intuitive touchscreen interface
With its advanced capabilities, the LightCycler 96 System can be used for a wide range of applications, including:
- Gene expression analysis
- Genotyping
- Microbial detection
- Mutation analysis
The system comes with powerful software that allows for easy experiment setup, data analysis, and reporting.
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