Thermo Fisher Scientific Axiom™ 2.0 Assay Owner's manual

Brand
Thermo Fisher Scientific
Model
Axiom™ 2.0 Assay
Type
Owner's manual
For Research Use Only. Not for use in diagnostic procedures.
Axiom™ 2.0 Assay 384HT-Array Format
SITE PREPARATION GUIDE
Automated Protocol for Beckman Biomek FXP
Publication Number 703269
Revision 5
The information in this guide is subject to change without notice.
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Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 3
Contents
CHAPTER 1 Biomek® FXP Target Prep Express Configuration . . . . . . . 4
Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Instrument specifications and settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Related Beckman Coulter documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Axiom™ 2.0 Target Prep Method for 384 Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Importing the method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
CHAPTER 2 Equipment and supplies required . . . . . . . . . . . . . . . . . . 10
Applied Biosystems equipment, software, reagents, and arrays required . . . . . . . . . . . . . . 10
Labware required for the Biomek FXP Target Prep Express. . . . . . . . . . . . . . . . . . . . . . . . 13
Axiom™ 2.0 Target Prep 384HT Samples Templates Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Other equipment, consumables, and reagents required . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Pre-amplification area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Oven requirements for target prep . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Thermal cyclers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Shakers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Plate centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Other common lab equipment required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Other reagents and gels required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Pipettes and tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Other Consumables Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
APPENDIX A Supplier contact list. . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Documentation and support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Related documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Customer and technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Limited product warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
4Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
1Biomek® FXP Target Prep Express
Configuration
Equipment
The Beckman Coulter Biomek® FXP Target Prep Express (Cat. No. A83103) is required
to run the Axiom™ 2.0 Assay for 384HT Samples with the automated target
preparation. This workstation includes the accessories, software and deck
configuration listed in Table 1. For information on upgrading a Biomek liquid handler
already at your site, contact Beckman Coulter. In addition to this workstation, the
components listed in Chapter 2 are also required. This chapter details the
configuration required when using Windows® 7 operating system.
Table 1 Biomek FXP Target Prep Express for Running the Axiom™ 2.0 Assay for 384HT Samples Workflow
Item Quantity Beckman
Cat. No.
Biomek FXP Target Prep Express
The part number for this workstation includes the components listed below.
1 A83103
•Biomek FX
P Dual Multichannel Span-8 1 A31844
• Biomek Windows 7 New Installation kit with Biomek Software 4.1 1 B40917
• Packaged Tip Loader 1 719356
• Span-8 Disposal ALP 1 719590
• Low Volume Disposable Mandrel Kit 8-Pack 1 A39380
• BFX 96-channel Disp, Tip Head, 200U 1 719368
• Pkg Static ALP Platform 4 719357
• 4 × 3 ALP Kit 1 719948
• Static Peltier ALP 1 A93938
• Shaking Peltier ALP 1 A93942
• Adapter, Deep Well Plate (required for Axiom™ assays) 1 A83050
• Adapter, Thin PCR (required for HT 3' IVT PLUS and HT WT PLUS assays) 1 A19709
• Frame for Modular Reservoir 4 372795
• 24-Position Tube Rack 1 373661
• Inserts, Tube, 11 mm (case of 25) 1 373696
• 24 Position Cold Tube Block
(referred to as Reagent Cold Block in this document)
1 A83054
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 5
Chapter 1 Biomek® FXP Target Prep Express Configuration
Instrument specifications and settings 1
Instrument specifications and settings
In order for the Axiom 2.0 assay for 384HT Samples to be successfully performed on
the Biomek FXP Target Prep Express Instrument the system must meet the following
specifications and be properly configured.
• Biomek FXP Target Prep Express system
– Hybrid dual armed Biomek FXP
– 96MC head left pod and Span-8 head right pod
– 1 mL syringes and low volume tubing on the Span8 system
– 384 Deep-Well Adapter (Cat. No. 101019) mounted on the Shaking Peltier
Device with notches facing the front
– ALPs secured to deck as shown in Figure 1
• System fluid is degassed water
• Clean compressed air is at 40–80 psi
• Computer workstation specifications
–Windows
® 7 operating system
– Internet Explorer® v8.0
–Microsoft
® Excel® 2010 software; 32-bit version ONLY software
– Biomek software v4.1.41 (version 4.1 build 41)
•Biomek software
– Make sure that the Affymetrix Deck is the default deck
– Make sure deck location SPelt96_1 has the device ShakingPeltier1 associated
with it
– Make sure deck location Pelt_1 has the device StaticPeltier1 associated with it
– Make sure deck location TR1 is ALP type Span8TrashRight and not
Span8TipTrashRight
• Check for a properly framed deck for both the Biomek FXP 96MC and Span8 pods
– Framing tolerance is 0.03 cm. Reframe if this is exceeded when checked
– If deck was reframed, save a current copy of the instrument file for backup
– Exit and restart Biomek software after installing Affymetrix Deck and after any
changes in deck framing in order to save the new values in the default
instrument (.bif) file
• Additional Pod Settings
–Navigate to: Instrument Hardware Setup Pod2 Additional Pod
Settings
– Pod 2 (Span-8) settings: Set System Trailing Airgap to 20 µL (Figure 2)
– Pod 1 (MC96) settings (Figure 3):
Check Always Rove at Z-Max box
Set Additional Roving Height to 0.45 cm or lower (default is 0.75 cm)
Set Gripper Offset in D Axis to 0.7 cm
Chapter 1 Biomek® FXP Target Prep Express Configuration
Instrument specifications and settings
6Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
1
Figure 1 Biomek FXP TPE System (top) and Deck Configuration (bottom)
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 7
Chapter 1 Biomek® FXP Target Prep Express Configuration
Instrument specifications and settings 1
Figure 2 Hardware Setup Changes for Span-8 Pod2
Figure 3 Hardware Setup Changes for Multichannel 96 Pod1
Chapter 1 Biomek® FXP Target Prep Express Configuration
Related Beckman Coulter documentation
8Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
1
Related Beckman Coulter documentation
•Biomek® Liquid Handler User’s Manual, Beckman Coulter Pub. No. 987834
•Biomek® FX Laboratory Automation Workstation Preinstallation Manual, Beckman
Coulter Pub. No. 719450, rev AH
•Biomek® Software User’s Manual Version 4.1, Beckman Coulter Pub. No. B30026AA
•Install Guide Biometra TRobot on Biomek FX Revision 2, 9/8/2014
Axiom™ 2.0 Target Prep Method for 384 Samples
The purpose of this section is to identify critical steps for importing the Axiom™ 2.0
Target Prep 384 Samples method into the Biomek® software for use on the Biomek® FXP
Target Prep Express.
Importing the
method
1. Run Setup.exe.
2. Click Setup.exe in method package to copy files from the package to the
following locations and import the Biomek method.
Table 2 Directory Location
Source Destination
\Root\*.* C:\Users\Public\Documents\Affymetrix\Axiom 2.0 384
\Images\ C:\Users\Public\Documents\Axiom 2.0 384\Images
\Misc\ C:\Users\Public\Documents\Axiom 2.0 384
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 9
Chapter 1 Biomek® FXP Target Prep Express Configuration
Axiom™ 2.0 Target Prep Method for 384 Samples 1
3. Set Method Preferences:
a. Locate and click the method branch named Axiom 2.0 Target Prep 384
Samples (Figure 4).
b. Barcode Tracking [Enabled] to input barcode during runtime deck setup.
• Log Name [Default/Other]: Name of barcode log text file
• Log Path [Default/Other]: Location of barcode log file
c. Set the Validate Reagent Module Barcodes run option to check for the correct
reagent module part number.
d. Set the Verify GeneTitan Labware Setup run option to check if the GT trays
were placed in the correct position on the deck.
e. Set the Prepare gel and OD plates for QC run option.
f. Set the Test Mode run option which disables the timers.
Figure 4
10 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2Equipment and supplies required
This chapter includes the supplier and ordering information for the equipment,
software, reagents, arrays, labware and other consumables that have been validated
for use with the Applied Biosystems™ Axiom™ 2.0 Assay for 384HT Samples.
Information on suppliers of the equipment and supples is listed in Appendix A,
ʺSupplier contact listʺ on page 27.
Applied Biosystems equipment, software, reagents, and arrays
required
Table 3 Applied Biosystems equipment, software, reagents and arrays required
Item Cat. No.
Equipment
GeneTitan™ Multi-Channel Instrument* Contact Thermo
Fisher Scientific,
Inc.
Software
GeneChip™ Command Console™ (AGCC) version 4.3 or later
Axiom Analysis Suite version 1.1 or later
Reagents
Axiom™ 2.0 384HT Reagent Kit 902245 (Sufficient
for one 384HT
array plate)
Arrays
Axiom™ 384HT Genotyping Solution Array Plates:
Please visit www.thermofisher.com for a complete list of array plates available for use with
the Axiom™ 2.0 Assay for 384 Samples.
* For a complete list of all equipment and supplies required for GeneTitan Instrument installation and operation, please consult the
GeneTitan™ Multi-Channel Instrument Site Preparation Guide (Pub. No. 08-0305).
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 11
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required 2
Consumables
Each Axiom™ 384HT High Volume Consumable Kit is sufficient to process 5 Axiom 384-
format plates.
• 10 x 384 Layout GeneTitan™ Stain Tray (Stain 1)
• 5 x 384 Layout Axiom™ Stain 2 Tray
• 5 x 384 Layout Axiom™ Stab. Tray
• 5 x 384 Layout Axiom™ Ligation Tray
• 5 x 384 Layout GeneTitan™ Hyb Tray
• 5 x 384 Layout GeneTitan™ Scan Tray
• 25 x 384 Layout GeneTitan™ Scan and Stain Tray Cover
These trays are required for processing Axiom 384 array plates on the GeneTitan™
Multichannel Instrument. Please use the new Axiom 384HT High Volume Consumable Kit
when running the Applied Biosystems™ Axiom™ 2.0 Assay for 384HT Samples.
902629
Axiom™ 384HT Consumable Kit for Biomek FXp (sufficient for 5 x 384 rxn runs)1 2
Each Kit Includes:
• 10 x 96 PCR Plate (Part No. 203015)
• 20 x 96 Deep-Well Square Plate (Part No. 203016)
• 15 x 384 Deep-Well 240 μL Plate (Part No. 203017)
• 15 x 384 Standard 120 μL Plate (Part No. 203018)
• 5 x 384 PCR Plate (Part No. 902288)
• 35 x Shallow 96 Full Reservoir (Part No. 203020)
• 5 x Deep 96 Full Reservoir (Part No. 203021)
902288
Axiom™ 384HT Consumable Kit for QC (sufficient for 10 x 384 rxn runs)2, Each Kit Includes:
• 10 x 384 UV Plate (Part No. 203022)
902289
Axiom™ 384HT Starter kit for Biomek FXP
Each Kit Includes:
• 1 x Thermoshake 384 Adapter (Part No. 101019)
• 1 x Plastic Plate Lid (Part No. 101018)
• Plate collar
• 1 set Reservoir Stickers (Part No. 90-0972)
• 1 x Cold Block Template #1 (Part No. 15-0448)
• 1 x Cold Block Template #2 (Part No. 15-0451)
• 1 x 2D Handheld Barcode Reader (Part No. 00-0179)
• 1 x External Barcode Reader Holder (Part No. 00-0093)
902290
Training Modules Available
Axiom™ 384HT Training Kit, Each Kit Includes:
•Axiom
™ 2.0 384HT Reagent Kit.
•Axiom
™ Human AIMs Array Plate
•Axiom
™ 384HT Consumable Kit for Biomek FXP
•Axiom
™ 384HT Consumable Kit for QC
•Axiom
™ 384HT GeneTitan™ MC Consumable Kit
•HapMap Samples
3
902291
1For quantity required per run, please refer to Table 4.
2The components of this kit may be purchased directly from the vendor, as listed in Table 4.
Table 3 Applied Biosystems equipment, software, reagents and arrays required (Continued)
Item Cat. No.
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required
12 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2
3Note: Per Thermo Fisher Scientific agreement with Coriell, all residual HapMap DNA samples must be destroyed immediately following
completion of training.
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 13
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required 2
Labware required
for the Biomek FXP
Target Prep Express
The labware required to run the Axiom™ 2.0 Assay for 384HT Samples Workflow on
the Biomek FXP Target Prep Express is listed in Table 4. Photographs of the labware are
provided in Table 5 on page 14.
Table 4 Labware for the Biomek FXP Target Prep Express
Item Quantity required Supplier Cat. No.
Pipette tips, barrier, 96 tips/rack1
AP96 P50 Tips (pink) 1152 tips
Beckman
Coulter
A21586
AP96 P250 Tips (aqua) 1440 tips 717253
Span-8 P250 Tips (green) 50 tips 379503
Span-8 P1000 Tips (yellow) 154 tips 987925
Plates
96 PCR Plate22 BIO-RAD HSP9631
384 Deep-Well 240 μL Plate2 33 E&K Scientific EK-2010-SBC
384 Standard 120 μL Plate23 Axygen/Corning P-384-
120SQ-C
384 PCR Plate2,3 1 Thermo Fisher
Scientific
902288
96 Deep-Well Square Plate2, 3 4 Thermo
Scientific
AB-0932
384 UV Plate4 1 E & K Scientific EK-30801
Reservoirs for Reagents
Shallow 96 Full Reservoir2 7 E & K Scientific EK-2036
Deep 96 Full Reservoir21 E & K Scientific EK-2035
Half Reservoir (75 mL module) 2
Beckman
Coulter
372786
Quarter Reservoir (40 mL module) 13 372790
Quarter Reservoir Divided by Width (19 mL module) 1 372792
Other
Microseal 'P' Replacement Pads BIO-RAD MSP-1003
Plastic Plate Lid Thermo Fisher
Scientific
101018
384 Deep-Well Adapter Thermo Fisher
Scientific
101019
1For pipette tips, the “Quantity required” column lists the total number of tips required and not the number of racks.
2 Available in the Axiom™ 384HT Consumable kit for Biomek FXP (Cat. No. 902288). See Table 3 on page 10 for details.
3 This plate is also part of the Axiom™ 384HT Consumable kit for Biomek® FXP. If purchased directly from the supplier, a unique barcode
must be added.
4 Available in the Axiom™ 384HT Consumable Kit for QC (Cat. No. 902289). See Table 3 on page 10 for details.
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required
14 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2
Photographs of the labware required
The photographs in Table 5 are provided to help visualize the labware required on the
Biomek FXP Target Prep Express to run the automated target preparation portion of the
Axiom™ 2.0 Assay Automated Workflow.
Table 5 Labware Used on the Biomek Workstation deck
Labware Supplier and Cat. No. Labware image
Biomek AP96 – P250 Pipette Tips
(aqua box; pre-sterile, barrier)
Beckman Coulter
Cat. No. 717253
Biomek Span P250 Pipette Tips
(green box; pre-sterile, barrier)
Beckman Coulter
Cat. No. 379503
Biomek Span P1000 Pipette Tips
(yellow box; pre-sterile, barrier,
conductive)
Beckman Coulter
Cat. No. 987925
Biomek Span P50 Pipette Tips
(pink box; pre-sterile, barrier)
Beckman Coulter
Cat. No. A21586
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 15
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required 2
Frame for reservoirs Beckman Coulter
Cat. No. 372795
Half reservoir
Half module, 75 mL capacity
Beckman Coulter
Cat. No. 372786
Quarter reservoirs
• Quarter module, 40 mL capacity
• Quarter module divided by width,
19 mL capacity each receptacle
Beckman Coulter
• Cat. No. 372790 (40 mL)
• Cat. No. 372792 (19 mL)
Table 5 Labware Used on the Biomek Workstation deck (Continued)
Labware Supplier and Cat. No. Labware image
Frame
Divided by width
19 mL capacity
Undivided
40 mL capacity
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required
16 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2
Reagent block, chilled to 4°C Beckman Coulter
Cat. No. A83054
24-position tube rack
with two 11 mm tube inserts in
positions A1 and B1.
Beckman Coulter
Cat. No. 373661 (rack)
Cat. No. 373696 (insert)
Plate collar Plate collar used with
ABgene 384-well PCR
plate. Contact your
Genotyping Technical
Sales Specialist for
additional plate collars.
96 PCR plate
Hard-Shell® Thin-Wall
96-Well Skirted PCR Plates
(available in multiple colors)
Bio-Rad
Cat. No. HSP9631
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
Table 5 Labware Used on the Biomek Workstation deck (Continued)
Labware Supplier and Cat. No. Labware image
Metal posts on block circled in red.
A1
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 17
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required 2
384 deep-well 240 μL plate1
Axygen® 384 Well Clear V-Bottom
240 μL Polypropylene Deep Well Not
Treated Plate, Sterile
Axygen/Corning
Cat. No.
P-384-240SQ-C-S
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
384 standard 120 μL plate
Axygen® 384 Well Clear V-Bottom
120 μL Polypropylene Deep Well Not
Treated Plate
Axygen/Corning
Cat. No. P-384-120SQ-C
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
384 PCR Plate2
ABgene 384 Well PCR Microplate,
Clear, Nonsterile
Thermo Fisher Scientific
Cat. No. AB0937
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
96 deep-well square plate1
ABgene® Mark II 96-Well Storage
Plate, Square Well, 2.2 mL
Thermo Scientific
Cat. No. AB-0932
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
Table 5 Labware Used on the Biomek Workstation deck (Continued)
Labware Supplier and Cat. No. Labware image
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required
18 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2
384 UV plate
384 Well, UV-Star Plates 120 μL,
Non-Sterile
E & K Scientific
Cat. No. EK-30801
Also available in the
Axiom™ 384HT
Consumable kit for QC
(Cat. No. 902289)
Shallow 96 full reservoir
Individual Well Format Reservoirs -
96 Well, Low Profile
E & K Scientific
Cat. No. EK-2036
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
Deep 96 full reservoir
Individual Well Format Reservoirs -
96 Well, Sterile
E & K Scientific
Cat. No. EK-2035
Also available in the
Axiom™ 384HT
Consumable kit for
Biomek FXP
(Cat. No. 902288)
Plastic plate lid Thermo Fisher Scientific
Cat. No. 101018
Also available in Axiom™
384HT Starter kit for
Biomek FXP
(Cat. No. 902290)
Table 5 Labware Used on the Biomek Workstation deck (Continued)
Labware Supplier and Cat. No. Labware image
Plastic plate lid
(top side)
Plastic plate lid
(underside)
P-pad
Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide 19
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required 2
P Pad
Microseal® ‘P’ Pad
PCR plate-sealing pad for
automation, resusable
Bio-Rad
Cat. No. MSP-1003
384 deep-well adapter
Plate adapter for Inheco
Thermoshake, fits Axygen
P-384-240SQ-C-S 384 deep-well
plate
Thermo Fisher Scientific
Cat. No. 101019
Also available in Axiom™
384HT Starter kit for
Biomek FXP (Cat. No.
902290)
1 The plates come unbarcoded from the vendor. Barcode labels should be applied before use. Barcoded versions of the plates are
available in the Axiom 384HT Consumable Kit for Biomek FXP.
2 At the current time this plate is unbarcoded. If desired, barcode label must be manually applied.
Table 5 Labware Used on the Biomek Workstation deck (Continued)
Labware Supplier and Cat. No. Labware image
Chapter 2 Equipment and supplies required
Applied Biosystems equipment, software, reagents, and arrays required
20 Axiom™ 2.0 Assay 384HT-Array Format Automated Protocol for Beckman Biomek FXP Site Preparation Guide
2
Axiom™ 2.0 Target
Prep 384HT
Samples Templates
Kit
The Tube Block and Reservoir Frames templates are provided in the Axiom™ 384HT
Starter Kit for Biomek FXP (Cat. No. 902290) and are ordered by your Thermo Fisher
Scientific FAS prior to beginning training. These templates are used with the
automated target prep protocol.
Tube block template
The Axiom 2.0 Target Prep 384 Samples Reagent Kit tube block templates were
designed to fit precisely onto the top of the Beckman Coulter Tube Block (Cat. No.
A83054). They are held in place by the metal posts on the block (Figure 5). Using these
templates helps to ensure the proper placement of reagent tubes onto the block for each
method.
Reservoir labels
The reservoir labels are stick-on labels for the modular reagent reservoir frames (used
at different stages in the automated workflow) and are provided in the Axiom™ 2.0
Target Prep 384HT Samples Templates Kit. These stick-on labels are color-coded to
match the colors found on the caps of the reagent tubes in the Axiom 2.0 384HT
Reagent Kit. Using these labels helps ensure the proper placement of reservoirs and
reagents for each step.
Figure 5 Axiom Reagent Templates for Tube Block
Tube block templates
Tube block
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Thermo Fisher Scientific Axiom™ 2.0 Assay Owner's manual

Brand
Thermo Fisher Scientific
Model
Axiom™ 2.0 Assay
Type
Owner's manual
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