Thermo Fisher Scientific GeneChip HT Pico Kit Assay Automated Workflow User guide

Brand
Thermo Fisher Scientific
Model
GeneChip HT Pico Kit Assay Automated Workflow
Type
User guide
For Research Use Only. Not for use in diagnostic procedures.
GeneChip™ HT Pico Kit Assay Automated
Workflow
SITE PREPARATION GUIDE
for use with:
GeneChip™ Expression Arrays
GeneChip™ HT Pico Kit
Beckman Coulter™ Biomek™ FXP Target Prep Express Automated Workstation
Catalog Numbers 902790 and 902791
Publication Number MAN0018105
Revision B.0
Manufacturer:
Thermo Fisher Scientific Baltics UAB |
V.A. Graiciuno 8, LT-02241 |
Vilnius, Lithuania
Products:
GeneChip™ HT Pico Kit
Manufacturer:
Aymetrix Pte Ltd |
7 Gul Circle #2M-01 |
Keppel Logistics Building |
Singapore 629563
Products:
GeneChip™ expression arrays
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018105
Revision Date Description
B.0 09 October 2020 Added additional Bio-Rad Hard Shell PCR Plate catalog number.
A.0 27 December 2018 •Initial release in Thermo Fisher Scientific document control system.
•Supersedes legacy Aymetrix publication number 703399.
•Updated to the current document template, with associated
updates to trademarks, logos, licensing, and warranty.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2020 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■CHAPTER 1 Biomek™ FXP Target Prep Express Automated
Workstation configuration .......................................................... 4
Equipment ..................................................................... 4
Instrument specifications and settings ............................................. 6
Other equipment required ....................................................... 10
Thermal cyclers ........................................................... 10
Spectrophotometer ........................................................ 10
Miscellaneous equipment required ........................................... 10
■CHAPTER 2 Materials required and GeneChip™ HT Pico v1.0
method installation ................................................................ 11
Part 1: Equipment, reagents, consumables, and labware required .................... 11
Equipment ................................................................ 11
Reagents ................................................................. 13
Consumables and labware .................................................. 15
Labware templates ......................................................... 21
Part 2: Biomek™ HT Pico v1.0 method installation .................................. 23
Time requirements ......................................................... 23
Install the method files ..................................................... 24
Import the method into the Biomek™ FXP Target Prep Express
Automated Workstation .................................................. 26
Make required edits to Biomek™ Software v4.1.41 (Build 41) .................... 30
Import and configure the Biometra T-Robot thermal cycler program .............. 34
Set Microsoft™ Excel™ 2010 options ......................................... 40
Validate the method ........................................................ 40
Error recovery ............................................................. 41
Test the method with a water run ............................................ 42
■Documentation and support ....................................................... 47
Related documentation ......................................................... 47
Customer and technical support ................................................. 48
Limited product warranty ........................................................ 48
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP3
Biomek™ FXP Target Prep
Express Automated Workstation
configuration
■Equipment ............................................................................ 4
■Instrument specifications and settings ................................................... 6
■Other equipment required ............................................................. 10
Equipment
The Beckman Coulter™ Biomek™ FXP Target Prep Express Automated Workstation is required to run
GeneChip™ High Throughput (HT) expression assays. This system includes the accessories, software,
and deck configuration listed in Table 1 and displayed in Figure 1. For information on upgrading a
Biomek™ liquid handler already at your site, contact Beckman Coulter™ Inc. Chapter 2, “Materials
required and GeneChip™ HT Pico v1.0 method installation” lists the components required to run the
GeneChip™ HT Pico Kit Assay Automated Workflow.
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Table 1 Biomek™ FXP Target Prep Express Automated Workstation components required for running
GeneChip™ HT methods for expression.
Item Quantity
Beckman
Coulter™
Cat. No.
Biomek™ FXP Target Prep Express Automated Workstation
The part number for this workstation includes the components listed below:
1 A83103
•Biomek™ FXP Dual Multichannel Span-8 1 A31844
•Biomek™ Win 7 New Installation kit with Biomek™ Software 4.1 1 B40917
•Packaged Tip Loader 1 719356
•Span-8 Disposal ALP 1 719590
•Low Volume Disposable Mandrel Kit, 8-pack 1 A39380
•BFX 96-channel Disp, Tip Head, 200U 1 719368
1
4GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Table 1 Biomek FXP Target Prep Express Automated Workstation components required for running
GeneChip HT methods for expression. (continued)
Item Quantity
Beckman
Coulter™
Cat. No.
•Pkg Static ALP Platform 4 719357
•4 × 3 ALP Kit 1 719948
•Static Peltier ALP 1 A93938
•Shaking Peltier ALP 1 A93942
•Deep well plate adapter (required for Axiom™ assays) 1 A83050
•Thin PCR adapter (required for expression assays) 1 A19709
•Frame for Modular Reservoirs 3 372795
•24-Position Tube Rack Holder 1 373661
•11-mm Diameter Tube Rack Inserts (case of 25) 1 373696
•Blue Heater/Chiller 24-well Block
(referred to as Reagent Cold Block in this document)
1 A83054
•Biometra T-Robot Integration - v4.1 1 969125
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Equipment 1
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP5
Instrument specifications and settings
To perform GeneChip™ HT Pico expression assays successfully on the Biomek™ FXP Target Prep
Express Automated Workstation instrument, the system must meet the following specifications and be
properly configured.
•Biomek FXP Target Prep Express system:
–Hybrid dual armed Biomek™ FXP
–96MC head left pod (Pod 1) and Span-8 head right pod (Pod 2)
–1-mL syringes and low-volume tubing on the Span-8 system
–Thin PCR adapter mounted on the Shaking Peltier Device
–Shaking Peltier ALP Labware Guides properly adjusted
–ALPs secured to deck. See Figure 1.
•Degassed water as system fluid
•Clean compressed air at 40–80 psi
•Computer workstation specifications:
–Microsoft™ Windows™ 7 operating system
–Internet Explorer™ v8.0
–Microsoft™ Excel™ 2010 (must be 32‑bit version)
–Biomek™ Software v4.1.31 (Build 31) or v4.1.41 (Build 41)
•Biomek™ Software configuration:
–Ensure that the Aymetrix™ deck is the default deck.
–Ensure that deck location SPelt96_1 has the device ShakingPeltier1 associated with it.
–Ensure that deck location Pelt_1 has the device StaticPeltier1 associated with it.
–Ensure that deck location TR1 is ALP type Span8TrashRight (not Span8TipTrashRight).
•Check for a properly framed deck for the Biomek™ 200-µL 96-Channel Disposable Pipetting Head
and the Biomek™ Span-8 Waste ALP pods.
–Framing tolerance is 0.03 cm. Reframe if this is exceeded.
–If the deck needs to be reframed, save a current copy of the instrument file for backup.
–Exit and restart the Biomek™ Software after installing the Aymetrix™ deck and after any
changes in deck framing. This saves the new values in the default instrument (.bif) file.
•Additional pod settings:
–Navigate to Instrument4Hardware Setup4Pod24Additional Pod Settings.
–Pod 2 (Span-8) settings (Figure 2)
Set System Trailing Airgap to 20 µL.
–Navigate to Instrument4Hardware Setup4Pod14Additional Pod Settings.
–Pod 1 (MC96) settings (Figure 3)
Check Always move to Z-Max when roving box.
Set the Additional Roving Height field to 0.45 cm or less (default is 0.75 cm).
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Instrument specifications and settings
1
6GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
•Set the adjustable labware corner guides on the Shaking Peltier ALP:
–Ensure that the thin adapter plate is installed on the peltier, if not, install the thin adapter plate.
–To adjust the newer (tan) adjustable corner guides the Shaking Peltier ALP must be removed
from the deck to allow access to the rear screws; to adjust the older (blue) adjustable corner
guides the shaking peltier can remain on the deck.
–The tan adjustable corner guides have 2 flathead screws present on each of the outer sides
of the four guides to perform adjustments to hold labware; the blue adjustable corner guides
have 2 hex screws on the top of each corner guide to perform adjustments to hold labware
–Place a Costar Polystyrene 96-Well U-bottom Plate onto the Shaking Peltier ALP
–Tan adjustable corner guides: tighten the flat head screws on the sides of each labware guide
(8 total screws) until a slight resistance is felt. Then tighten each screw an additional 1/2 turn.
–Blue adjustable corner guides: adjust the corner guide screws until labware is held snugly.
–Ensure that the Costar Polystyrene 96-Well U-bottom Plate is held snugly but can be removed
from the Shaking Peltier ALP.
–Ensure that a Bio-Rad™ Hard-Shell™ 96-Well PCR Plate plate can be placed into and removed
from the Shaking Peltier ALP.
–Reinstall the Shaking Peltier ALP on the deck and reframe the position for Pod 1 if necessary.
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Instrument specifications and settings 1
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP7
Figure 1 Biomek™ FXP Target Prep Express Automated Workstation system (top) and deck configuration
(bottom).
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Instrument specifications and settings
1
8GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Figure 2 Hardware setup changes for the span-8 ALP on Pod 2.
Figure 3 Hardware setup changes for the 96-channel head on Pod1.
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Instrument specifications and settings 1
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP9
Other equipment required
Thermal cyclers
The GeneChip™ HT Pico v1.0 method has 2 thermal cycler options for reaction incubations: an on-deck
Biometra™ TRobot 96 Thermocycler or an o-deck thermal cycler.
For on-deck incubation using a Biometra™ TRobot 96 Thermocycler (Biometra™ Cat. No. 050-991), the
thermal cycler should be purchased directly from a local Biometra™ distributor. For information, see
analytikjena.com. The Biometra™ TRobot 96 Thermocycler integration solution must be purchased
separately from Beckman Coulter™.
To use an o-deck thermal cycler, ensure that the thermal cycler can accept the full-skirted Bio-Rad
Hard-Shell 96 PCR plate. If the hybridization-ready target will be prepared for cartridge arrays, ensure
that the thermal cycler can accept FrameStar™ Break-A-Way PCR Plates. See Table 9.
Spectrophotometer
The GeneChip™ HT Pico v1.0 method has 2 options for measuring nucleic acid concentration: a UV/Vis
plate reader or a NanoDrop™ spectrophotometer (or equivalent instrument). NanoDrop™ instruments can
be purchased from Thermo Fisher Scientific. There are no unique plate reader requirements except that
the plate reader must accommodate Greiner UV-Star plates. See Table 9.
Miscellaneous equipment required
"MLS" indicates that the material is available from fisherscientific.com or another major laboratory
supplier.
Table 2 Other equipment required
Item Source
Benchtop cooler, –20°C MLS
Centrifuge for 96-well plates and 1.5-mL and 2.0-mL tubes MLS
Freezer, –20°C, not frost-free MLS
Ice bucket MLS
Pipette with pipette tips MLS
Refrigerator, 2–8°C MLS
Vortex mixer MLS
Chapter 1 Biomek™ FXP Target Prep Express Automated Workstation configuration
Other equipment required
1
10 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Materials required and GeneChip™
HT Pico v1.0 method installation
■Part 1: Equipment, reagents, consumables, and labware required .......................... 11
■Part 2: Biomek™ HT Pico v1.0 method installation ........................................ 23
“Part 1: Equipment, reagents, consumables, and labware required” on page 11 of this chapter lists
the part numbers and suppliers for the equipment, reagents, consumables, and labware that have been
verified for use with the GeneChip™ HT Pico Kit on the Beckman Coulter™ Biomek™ FXP Target Prep
Express Automated Workstation. “Part 2: Biomek™ HT Pico v1.0 method installation” on page 23 of
this chapter provides detailed instructions for the installation and validation of the HT Pico v1.0 method
for the Biomek™ FXP Target Prep Express Automated Workstation.
Part 1: Equipment, reagents, consumables, and labware
required
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Equipment
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Table 3 Equipment required for target preparation.
Quantity Item Source
1 Biomek™ FXP Target Prep Express Automated Workstation Beckman Coulter™
A83103
1 24-Position Tube Rack Holder Beckman Coulter™
373661
11-mm Diameter Tube Rack Inserts (11 mm, white, case of 25) Beckman Coulter™
373696
1 Reagent Cold Block, 24 Well Block Beckman Coulter™
A83054
1 Thin PCR adapter (installed on the Shaking Peltier ALP) Beckman Coulter™
A19709
2
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP11
Table 3 Equipment required for target preparation. (continued)
Quantity Item Source
1 Centrifuge (for tube and plate) MLS
1 Pipettes MLS
1 Spectrophotometer (plate, single channel, or multichannel) MLS
1 Thermal cycler (for optional o-deck incubation) MLS
As
needed
Adhesive PCR plate seals (for optional o-deck incubation) MLS
1 Vortex mixer MLS
Table 4 Expression installation kits required for the HT Pico method.
Quantity Item Source
1 GeneChip™ HT 3' IVT PLUS and WT PLUS Core Installation Kit ,
components:
•Alpaqua™ Magnetic Plate
•Alpillo™ Plate Cushion
•Arched auto-sealing lids with wide tabs (package of 4)
902453
1 GeneChip™ HT Pico Installation Kit, components:
•HT Pico reagent cold block template
•HT Pico reservoir frame stickers
•PCR tube holder
902901
HT Pico v1.0 Biomek™ method file and support files 600757
(Installed by Thermo
Fisher Scientific FAS)
Table 5 Equipment required for array processing.
Item Source
GeneChip™ System for Cartridge Arrays
•GeneChip™ Hybridization Oven 645
•GeneChip™ Fluidics Station 450
•GeneChip™ Scanner 3000 7G System
•00-0331 (110/220V)
•00-0079
•00-0213
GeneChip™ AutoLoader with External Barcode Reader 00-0090 (GCS 3000 7G S/N 501)
00-0129 (GCS 3000 7G S/N 502)
GeneTitan™ Multi-Channel (MC) Instrument Contact Thermo Fisher Scientific
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required
2
12 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Reagents
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Table 6 Contents and storage: GeneChip™ 3′ IVT Pico Kit (Cat. No. 902790, 30 reactions) and
GeneChip™ HT Pico Kit (Cat. No. 902791, 96 reactions).
Component Storage
Pico Amplification Kit, Module 1: cRNA Step
Pico First-Strand Enzyme
–20°C
Pico First-Strand Buer
Pico Cleanup Reagent
Pico 3’ Adaptor Enzyme
Pico 3’ Adaptor Buer
Pico PCR Enzyme
Pico PCR Buer
Pico IVT Enzyme
Pico IVT Buer
Poly-A Control Stock
Nuclease-free Water Any temperature[1]
Pico Amplification Kit, Module 2: cDNA Step
Pico 2nd-Cycle Primers
–20°C
Pico 2nd-Cycle ds-cDNA Enzyme
Pico 2nd-Cycle ds-cDNA Buer
Pico Frag. & Label Enzyme
Pico Frag. & Label Buer
Pico Amplification Kit, Module 3
Purification Beads
4°C[2]
Pico Hydrolysis Buer
Pico Neutralization Buer
Control HeLa RNA
Control RNA (100 ng/μL HeLa total RNA) –20°C
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required 2
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP13
Table 6 Contents and storage: GeneChip 3′ IVT Pico Kit (Cat. No. 902790, 30 reactions) and
GeneChip HT Pico Kit (Cat. No. 902791, 96 reactions). (continued)
Component Storage
GeneChip™ Hybridization Control Kit
20X Hybridization Controls
–20°C
3 nM Control Oligo B2
Tubes Organizer: Plastic vinyl template for organization and storage of components in 9 x 9 array, 81-
places square wells, 5 1/4 in. x 5 1/4 in (for example, Nalgene™ Polycarbonate 9 x 9 CryoBox, Cat. No.
5026-0909 ).
[1] Store the Nuclease-free Water at –20°C, 4°C, or room temperature.
[2] Do not freeze.
Table 7 Additional reagents required.
Item Source
GeneChip™ Hybridization, Wash, and Stain Kit (30 reactions, for cartridge arrays) 900720
GeneTitan™ Hybridization, Wash, and Stain Kit for WT Array Plates (96 reactions) 901622
GeneTitan™ Hybridization, Wash, and Stain Kit for 3’ IVT Arrays (96 reactions) 901530
DNAZap™ PCR DNA Degradation Solutions AM9890
RNaseZap™ RNase Decontamination Solution AM9780
Nuclease-free Water (for elution, dilution and preparing 80% Ethanol Wash Solution):
•~45 mL for 1 × 24 reactions, and
•~120 mL for 1 × 96 reactions
MLS
100% Ethanol (molecular biology grade or equivalent) for preparing 80% Ethanol
Wash Solution:
•~20 mL for 1 × 24 reactions, and
•~50 mL for 1 × 96 reactions
MLS
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required
2
14 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Consumables and labware
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Thermo Fisher Scientific does not oer a consumables kit specifically for use with the HT Pico v1.0
method. The HT WT PLUS Consumables for Biomek™ FXP Target Prep Express (Cat. No. 902508),
when supplemented with 2 additional Bio-Rad™ 'P' pads (Cat. No. MSL2032 ), is sucient to perform 4
complete HT Pico assays for cartridge arrays or 3′ IVT array plates.
When supplemented with 2 additionalBio-Rad™ 'P' pads and 4 additional Bio-Rad Hard-Shell PCR
plate, clear well (Cat. No. HSP9631 or HSP9601), the kit will be sucient to perform 4 complete HT
Pico assays for WT or Clariom™ S array plates.
Table 8 Beckman Coulter™ tips and labware required for 1 full run of the GeneChip™ HT Pico Kit on the
Biomek™ FXP Target Prep Express Automated Workstation.
Item Packaging Total 24X Total 96X Source
Biomek™ P50 Sterile Filtered Tips (magenta
box)[1]
96/box
10 boxes/case
12 boxes 14 boxes Beckman
Coulter™
A21586
Biomek™ P250 Sterile Filtered Tips (aqua
box), for AP96 multichannel pipettor
•Plates
•Cartridges
96/box
10 boxes/case
•6 boxes
•5 boxes
•6 boxes
•5 boxes
Beckman
Coulter™
717253
Biomek™ P250 Span-8 Sterile Filtered
Tips(green box), for span-8 pipettor
96/box
10 boxes/case
1 box 2 boxes Beckman
Coulter™
379503
Biomek™ P1000 Span-8 Conductive Sterile
Filtered Tips (yellow box) or Biomek™ P1000
Span-8 Nonconductive Sterile Filtered Tips
(orange box), for span-8 pipettor[2]
96/box
5 boxes/case
2 boxes 2 boxes Beckman
Coulter™
987925 or
B01124
Quarter Reservoir, Divided by Width, 19 mL
per section
•Plates
•Cartridges
48 units/case
•6 units
•4 units
•6 units
•4 units
Beckman
Coulter™
372792
Quarter Reservoir, 40 mL
•Plates
•Cartridges
48 units/case
•5 units
•4 units
•5 units
•4 units
Beckman
Coulter™
372790
Frame for Modular Reservoirs 1 3 reusable
frames
3 reusable
frames
Beckman
Coulter™
372795
[1] Two fewer boxes of this tip required for NanoDrop™ quantification option for method parts 2 and 4.
[2] The HT Pico v1.0 method can use conductive and nonconductive P1000 Span-8 tips interchangeably.
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required 2
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP15
Table 9 Consumables and labware used on the Biomek™ FXP Target Prep Express Automated Workstation
deck.
Item Source Image
Biomek™ P50 Sterile Filtered Tips
(magenta box)
Beckman Coulter™ A21586
Biomek™ P250 Sterile Filtered Tips
(aqua box)
Beckman Coulter™ 717253
Biomek™ P250 Span-8 Sterile
Filtered Tips(green box)
Beckman Coulter™ 379503
Biomek™ P1000 Span-8 Conductive
Sterile Filtered Tips (yellow
box) or Biomek™ P1000 Span-8
Nonconductive Sterile Filtered Tips
(orange box)
Beckman Coulter™
987925 (yellow) or
B01124 (orange)
Reservoir, Quarter
•Quarter Reservoir, 40 mL
•Quarter Reservoir, Divided by
Width, 19 mL per section
Beckman Coulter™
•372790
•372792
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required
2
16 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Table 9 Consumables and labware used on the Biomek FXP Target Prep Express Automated Workstation
deck. (continued)
Item Source Image
Frame for Modular Reservoirs
(3 total needed for HT Pico Method,
reusable for subsequent runs)
Beckman Coulter™ 372795
96-well deep well microplate,
polypropylene, 2 mL, non-sterile
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
(or Fisher Scientific™ AB0932 )
Greiner U-bottom (round) plate,
polypropylene
Used as a distribution plate for
cartridge hybridization mix and is not
interchangeable with the polystyrene
U-bottom plate.
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
(or Fisher Scientific™ 07-000-146)
Bio-Rad Hard-Shell PCR plate, clear
well
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
Note: One additional plate per run
required for WT or Clariom™ S array
plate option.
(or Bio-Rad™HSP9631 or HSP9601 )
A
B
C
D
E
F
G
H
1
2
3
4
5
6
7
8
9
10
11
12
A
B
C
D
E
F
G
H
1
2
3
4
5
6
7
8
9
10
11
12
Greiner UV-Star plate, nonsterile,
clear, flat bottom
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
(or Fisher Scientific™ 07-000-407)
Costar 96-Well U-bottom plate,
polystyrene
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required 2
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP17
Table 9 Consumables and labware used on the Biomek FXP Target Prep Express Automated Workstation
deck. (continued)
Item Source Image
24-well plate, 10 mL, square well,
pyramid bottom, polypropylene
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
(or Seahorse Bioscience S30024)
'P' Pad for lid, autosealing
microplates, arched with Microseal
Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
Note: One new 'P' Pad is required
per run for HT Pico.
(or Bio-Rad™ MSP1003, MSL2032
with lid)
FrameStar Break-A-Way plate Included in GeneChip™ HT WT
PLUS Consumables for Biomek™
FXP Target Prep Express Automated
Workstation, 902508
(or Thomas Scientific 4ti-1000/B)
Nalgene™ Polycarbonate 9 x 9
CryoBox
5026-0909
Alpaqua™ Magnetic Plate Included in GeneChip™ HT 3' IVT
PLUS and WT PLUS Core Installation
Kit , 902453
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required
2
18 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Table 9 Consumables and labware used on the Biomek FXP Target Prep Express Automated Workstation
deck. (continued)
Item Source Image
Alpillo™ Plate Cushion Included in GeneChip™ HT 3' IVT
PLUS and WT PLUS Core Installation
Kit , 902453
Arched auto-sealing lids with wide
tabs
Included in GeneChip™ HT 3' IVT
PLUS and WT PLUS Core Installation
Kit , 902453
Blue Heater/Chiller 24-well Block
Block must be chilled to 4°C before
use.
Beckman Coulter™ A83054
HT Pico Cold Block Template
The template is designed for the
GeneChip™ HT Pico Kit and fits
precisely onto the reagent cold
block. The cold block template helps
ensure proper placement of reagent
tubes into the block for each part of
the assay.
Included in the GeneChip™ HT Pico
Installation Kit, 902901.
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required 2
GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP19
Table 9 Consumables and labware used on the Biomek FXP Target Prep Express Automated Workstation
deck. (continued)
Item Source Image
HT Pico Reservoir Frame Stickers
Place modular reservoir template
stickers on the front of the modular
reservoir frames to ensure proper
placement of modular reservoirs and
reagents.
Included in the GeneChip™ HT Pico
Installation Kit, 902901.
PCR Tube Holder
For use with FrameStar™ Break-A-
Way PCR Plate in cartridge array
hybridization setup.
Included in the GeneChip™ HT Pico
Installation Kit, 902901.
(or Bio-Rad™ TRC-9601)
24-Position Tube Rack Holder with
11-mm Diameter Tube Rack Inserts
Beckman Coulter™
373661 (rack)
373696 (inserts)
Thin PCR Adapter
This adapter is typically installed
on the Shaking Peltier ALP by
a Beckman Coulter™ field service
technician during new system
installation or a system upgrade.
Ensure that you have the thin PCR
adapter installed on the Shaking
Peltier ALP prior to running this
assay.
Beckman Coulter™ A19709
GeneTitan™ Hybridization Tray Part. No. 500867
(GeneTitan™ trays and lids are
provided with the array plate when
ordered. The part number provided
here is for identification purposes
only.)
Chapter 2 Materials required and GeneChip™ HT Pico v1.0 method installation
Part 1: Equipment, reagents, consumables, and labware required
2
20 GeneChip™ HT Pico Kit Assay Automated Workflow Site Preparation Guide—Biomek™ FXP
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
Page is loading ...
  • Page 1 1
  • Page 2 2
  • Page 3 3
  • Page 4 4
  • Page 5 5
  • Page 6 6
  • Page 7 7
  • Page 8 8
  • Page 9 9
  • Page 10 10
  • Page 11 11
  • Page 12 12
  • Page 13 13
  • Page 14 14
  • Page 15 15
  • Page 16 16
  • Page 17 17
  • Page 18 18
  • Page 19 19
  • Page 20 20
  • Page 21 21
  • Page 22 22
  • Page 23 23
  • Page 24 24
  • Page 25 25
  • Page 26 26
  • Page 27 27
  • Page 28 28
  • Page 29 29
  • Page 30 30
  • Page 31 31
  • Page 32 32
  • Page 33 33
  • Page 34 34
  • Page 35 35
  • Page 36 36
  • Page 37 37
  • Page 38 38
  • Page 39 39
  • Page 40 40
  • Page 41 41
  • Page 42 42
  • Page 43 43
  • Page 44 44
  • Page 45 45
  • Page 46 46
  • Page 47 47
  • Page 48 48
  • Page 49 49
  • Page 50 50

Thermo Fisher Scientific GeneChip HT Pico Kit Assay Automated Workflow User guide

Brand
Thermo Fisher Scientific
Model
GeneChip HT Pico Kit Assay Automated Workflow
Type
User guide
Download PDF
report

Ask a question and I''ll find the answer in the document

Finding information in a document is now easier with AI

Related papers

Other documents