Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

Brand
Thermo Fisher Scientific
Model
Axiom 2.0 Assay 384HT Array Format Automated Workflow
Type
User guide
For Research Use Only. Not for use in diagnostic procedures.
Axiom™ 2.0 Assay 384HT Array Format
Automated Workflow
SITE PREPARATION GUIDE
for use with:
Axiom™ 384HT Array Plate
Axiom™ 2.0 384HT Reagent Kit
Biomek™ i7 Automated Workstation
Catalog Number902245
Publication Number MAN0017556
Revision E.0
Thermo Fisher Scientific Baltics UAB |
V.A. Graiciuno 8, LT-02241 |
Vilnius, Lithuania
Products manufactured at this site:
•Axiom™ 2.0 384HT Reagent Kit
Aymetrix Pte Ltd |
7 Gul Circle #2M-01 |
Keppel Logistics Building |
Singapore 629563
Products manufactured at this site:
•Axiom™ myDesign™ Array Plate
•Axiom™ 384HT Array Plate
Revision history:MAN0017556 E.0 (English)
Revision Date Description
E.0 5 June 2023 •Corrected a part number typo error for a component of the Axiom™ 384HT Consumables Kit for
Biomek™i7‑AB (Cat. No.952472)
•Added an additional part number for a component of the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB
(Cat. No.952472)
•Updated version numbers for required software.
•Updated legacy Aymetrix document numbers with new Thermo Fisher Scientific publication numbers.
D.0 20 April 2022 •Updated to add information for the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat.No.952472).
•Added an appendix to explain the dierences between the Axiom™ 384HT consumable kits and method
dierences for the Biomek™ i7 Automated Workstation. AppendixA, “Consumable kit and method version
dierences”.
C.0 19 January 2021 Updated to add information for the Axiom™ 384HT Consumables Kit for Biomek™i7 v2 (Cat. No. 952433).
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Microsoft,
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and/or other countries. Allegra and Biomek are trademarks of Beckman Coulter, Inc. REPLI-g is a trademark of QIAGEN GmbH. Axygen
is a trademark of Corning Incorporated. Bio-Rad, Hard-Shell, and Microseal are trademarks of Bio-Rad Laboratories, Inc. Zebra is a
trademark of ZIH Corporation. BINDER is a trademark of BINDER GmbH. Boekel Scientific and Jitterbug are trademarks of Boekel
Scientific. Thomas Scientific is a trademark of Thomas Scientific, LLC. Eppendorf is a registered trademark of Eppendorf AG.
©2018-2023 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■CHAPTER1Biomek™ i7 Automated Workstationconfiguration ................ 4
Biomek™i7components ......................................................... 4
Biomek™i7 configuration andsettings ............................................. 5
Axiom™ 2.0 Assay 384 HT Array Formatmethod .................................... 6
Import themethods ......................................................... 6
Set the method preferences .................................................. 8
Thermoshake plate adapter installation ........................................... 11
Install the Thermoshake plate adapter ........................................ 12
■CHAPTER2Assay equipment and supplies required .......................... 13
Equipment required ............................................................ 13
Applied Biosystems™ equipment required ..................................... 13
Oven requirements ......................................................... 13
Spectrophotometer ........................................................ 14
Thermal cycler ............................................................ 14
Shakers .................................................................. 14
Platecentrifuge ............................................................ 15
Other equipment required ................................................... 16
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array
Format AutomatedWorkflow .................................................. 16
Applied Biosystems™ software, reagents, arrays, and consumables required ...... 16
Labware required for the Biomek™ i7 Automated Workstation ................... 19
Templates for Axiom™ 2.0 Assay 384 HT Array Format on the Biomek™i7 ......... 28
Preamplification/amplification staging area .................................... 30
Other reagents and supplies required ........................................ 31
■APPENDIXAConsumable kit and methodversion dierences ............... 32
Consumable kit and method versiondierences ................................... 32
■APPENDIXBDocumentation and support ...................................... 33
Relateddocumentation ......................................................... 33
Customer and technical support ................................................. 35
Limited product warranty ........................................................ 35
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 3
Biomek™ i7 Automated Workstation
configuration
The Beckman Coulter™ Biomek™ i7 Automated Workstation is required to run this version of the
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow with the automated target preparation.
This workstation includes the accessories, software, and deck configuration listed in Table1. In addition
to this workstation, the components that are listed in Chapter 2, “Assay equipment and supplies
required” are also required. This chapter details the Biomek™i7 configuration that is required when
using Windows™ 10 operating system.
Biomek™i7 components
Table1Biomek™ i7 Automated Workstation components
3Item Quantity Beckman Coulter™
Cat.No.
 Biomek™i7 Single-Bridge with Multichannel Pod Automated Liquid
Handler Workstation with Biomek™ Software & Controller 1 B87581
 Multichannel-96 pipetting head, 5-1,200 μL 1 B87589
 1x1 Static ALP Kit 39 B87477
 Trash ALP 1 B87483
 Shaking Peltier ALP 4 A93942
 Vibration Isolation Plate ALP 4 C02750
 1x1 Tip Load Adapter Plate ALP (5 pk) 4 C02751
 Mobile Workstation, i7 1 C02613
1
4Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
Biomek™i7 configuration and settings
In order for the Axiom™ 2.0 Assay 384 HT Array Format to be successfully performed on the Biomek™i7
Automated Workstation, the system must meet the following specifications and be properly configured.
•Biomek™ i7 Automated Workstation
–96MC (model 5, capacity 1,200µL)
–384 Deepwell Adapter (Part No. 203253, available from the starter kit or upgrade kit) installed
on deck location Peltier1
–ALPs fastened to deck as shown in Figure1
Figure1Biomek™i7 deck positions.
•Computer workstation specifications
–Windows™ 10 operating system
–Biomek Software v5.1.9
•Biomek Software
–Ensure that the i7_Axiom Deck is the default deck
–Ensure deck locations Peltier1, Peltier2, Peltier3, Peltier4 have the device InhecoSingleTEC1,
InhecoSingleTEC2, InhecoSingleTEC3, InhecoSingleTEC4 associated with them.
–Ensure that deck location TR1 is ALP type TrashLeftSide
•Check for a properly framed deck for the Biomek i7 96MC
–Framing tolerance is 0.03 cm. Reframe if this tolerance is exceeded when checked
–If deck was reframed, save a current copy of the instrument file for backup
–Exit and restart Biomek Software after installing i7_Axiom Deck and after any changes in deck
framing to save the new values in the default instrument (.bif) file
•Ensure that a −0.65-cm labware oset is found for the BCTubeBlock_2mlTubes labware type on
all four Peltiers. To view and check the oset:
a. From the Utilities tab, click Deck Editor.
b. In the graphical deck representation, double‑click one of the four peltier positions.
c. In the Position Properties window, click More4Per-labware Osets.
Chapter1Biomek™ i7 Automated Workstation configuration
Biomek™i7 configuration and settings 1
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 5
d. Select BCTubeBlock_2mlTubes in the left column. Ensure that the Z oset value is set to
−0.65 cm.
e. Click OK twice to return to the graphical deck representation and repeat for each remaining
three peltiers.
Axiom™ 2.0 Assay 384 HT Array Format method
The purpose of this section is to identify critical steps for importing the Axiom™ 2.0 Assay 384 HT Array
Format method into the Biomek™ Software for use on the Biomek™ i7 Automated Workstation.
Import the methods
1. Copy the following folders from method package on the USB drive and transfer them to
C:\Users\Public\Documents.
•Axiom 2.0 384
•Axiom 2.0 Methods_v1.1
2. If the Biomek Software is open, close it before proceeding to ensure that internal data tables are in
their default state.
3. Open the Biomek™ Software using one of the following methods:
•Double‑click the Biomek icon on the desktop.
•In the Windows Search box, enter Biomek 5.
4. Click File4New4Project, then name the project Axiom 384 on i7.
Chapter1Biomek™ i7 Automated Workstation configuration
Axiom™ 2.0 Assay 384 HT Array Format method
1
6Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
5. Ensure that the Axiom 384 on i7 project is currently open by checking the project name
that is displayed in the bottom left of the Biomek™ Software window. If it is not open, click
File4Open4Project, then select Axiom 384 on i7.
6. Click File4Import.
7. In the Import Method window, navigate to C:/Users/Public/Documents/TFS/Axiom 2.0
384 Methods_v1.1 folder containing the six methods.
8. Select a method to import, then click OK. If this method is the first being imported, click OK on the
Import Project prompt to update the project.
9. Repeat step8 until all six methods have been imported.
Chapter1Biomek™ i7 Automated Workstation configuration
Axiom™ 2.0 Assay 384 HT Array Format method 1
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 7
Set the method preferences
IMPORTANT! The method preferences must be selected before starting the method. These settings
are not prompted for at runtime. Typically, the Thermo Fisher Scientific field application scientist (FAS)
sets the method preferences during method installation.
1. Open the Biomek™ Software using one of the following methods:
•Double‑click the Biomek 5 icon on the desktop.
•In the Windows Search box, enter Biomek 5.
2. Click File4Open.
3. For Look in of the Open Method window, select Axiom 384 on i7.
4. Click to select a method, then click OK.
Chapter1Biomek™ i7 Automated Workstation configuration
Axiom™ 2.0 Assay 384 HT Array Format method
1
8Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
5. In the Method View panel, click the line that reads Click Here to Select Method Preferences
(Figure2).
Figure2Click to select method preferences.
Chapter1Biomek™ i7 Automated Workstation configuration
Axiom™ 2.0 Assay 384 HT Array Format method 1
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 9
6. Click to select the method preferences.
1
1Method v1.1 information.
Logging options.
•Barcode Tracking: This feature is enabled by default. It is highly recommended to use the
barcode tracking feature to ensure accuracy in processing the samples. Consult with a FAS
if it is necessary to disable the barcode tracking feature. When selected the barcode tracking
feature performs the following actions:
–Prompts you to enter the barcode information on sample plates, Axiom™ reagent
modules, and GeneTitan™ consumables.
–Records barcode information into a log file.
–Ensures that the correct amplification time for the samples has elapsed (minimum of 22
hours) before starting the fragmentation and precipitation step.
–Ensures that the splitting and reassembly of samples are correctly associated to the
starting sample plate.
Note: If using custom barcodes for plates, only use alpha-numeric, hyphen, or underscore
characters.
Chapter1Biomek™ i7 Automated Workstation configuration
Axiom™ 2.0 Assay 384 HT Array Format method
1
10 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
Note: The barcode tracking feature is intended to track samples that go through the entire
target preparation process on one instrument.
•Log Name: The log name is the name of the barcode log file. The default name is
BarcodeLog[InstSN].txt, where "InstSN" is the serial number of the instrument. Naming of
the log file is possible by selecting Other in the dropdown list for the setting. If using a custom
log name, ensure that the name ends with ".txt" otherwise the file type will not be associated
correctly. After entering a custom name, click the Apply to the right of the log name field.
•Log Path: The log path is the barcode log file location. The log path is:
C:\Users\Public\Documents\Axiom 2.0 384\
Custom run options.
•Verify Reagent Module barcodes: When selected this feature ensures that the correct
Axiom™ reagent is used in the specified target preparation step.
•Verify GeneTitan Labware Setup: When selected, this feature ensures that the GeneTitan™
reagents that are prepared on the Biomek™i7 are correctly associated with the GeneTitan™
plate type.
•Prepare gel and OD plates for QC: the workstation prepares two plates of resuspended
samples that are properly diluted for the fragmentation gel QC and OD quantification process
control checks.
IMPORTANT! For troubleshooting and support purposes, we strongly recommend that you
perform the gel QC and OD quantification process controls after resuspension.
•Test Mode: Select this option to skip all the incubation timers in a step. If selected, a prompt
is displayed asking you to ensure that you want to run a step in test mode. Use this option to
perform runs using deionized water only, not actual reagents or samples.
CAUTION! Never process samples in test mode. Processing samples in test mode results
in assay failure and loss of samples and reagents.
Note: Perform verification tasks listed on the Axiom™ 384 on i7 System Readiness Checklist.
Thermoshake plate adapter installation
The Thermoshake 384 deepwell plate adapter that is used with the Axiom™ 2.0 Assay 384HT Array
Format Automated Workflow method is designed specifically to fit the 384 deepwell 250‑μL plate.
Proper installation is critical to ensuring even heating of the samples and proper liquid transfer in the
fragmentation step.
The Thermoshake 384 Deepwell Plate Adapter is a component of the Axiom™ 384HT Starter Kit for
Biomek™i7‑AB (Cat. No. 952469) or the Axiom™ 384HT Upgrade Kit for Biomek™i7‑AB (Cat. No.
952471).
Chapter1Biomek™ i7 Automated Workstation configuration
Thermoshake plate adapter installation 1
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 11
Install the Thermoshake plate adapter
1. Ensure that the 2 notched corners are oriented facing the front of the Thermoshake 384 Deepwell
Plate Adapter.
Figure3Thermoshake 384 Deepwell Plate Adapter orientation on the Biomek™i7 deck.
2. Press down firmly to ensure that adapter is evenly seated on the shaker and that the bottom
surface of the Thermoshake adapter is flush on the top surface of the shaker.
Chapter1Biomek™ i7 Automated Workstation configuration
Thermoshake plate adapter installation
1
12 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
Assay equipment and supplies
required
This chapter includes the supplier and ordering information for the equipment, software, reagents,
arrays, labware, and other consumables that have been verified for use with the Applied Biosystems™
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow.
Equipment required
Applied Biosystems™ equipment required
✓Item Cat.No.
 GeneTitan™ Multi-Channel Instrument[1] Contact Thermo Fisher Scientific
 Digital Scanner/Barcode Reader (to enable barcode tracking)
External Barcode Reader Holder (to enable barcode tracking)
74-0016
00-0093
[1] For a complete list of all equipment and supplies required for GeneTitan™ Multi-Channel Instrument installation and operation, consult
the GeneTitan™ Multi-Channel Instrument Site Preparation Guide (Pub. No. MAN0025571)
Oven requirements
We recommend using either the Thermo Scientific™ Heratherm™ Advanced Protocol Microbiological
Incubator, BINDER™ ED 56 Drying and Heating Chamber, or the BINDER™ BD 56 Standard-Incubator
with natural convection that are listed in the following table. If another oven is used, it must meet the
following requirements.
•Be able to maintain a constant temperature of 37°C for at least 24 hours, and have a temperature
accuracy of ±1°C, and
•Be able to maintain a constant temperature of 48°C for at least 24 hours, and have a temperature
accuracy of ±1°C.
Unless otherwise indicated, all materials are available through thermofisher.com.
✓Item Source
 Thermo Scientific™ Heratherm™ Advanced Protocol Microbiological
Incubator, capacity 66 L
•120V, 60 Hz
•230V, 50/60 Hz
  
•51028066
•51028133
2
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 13
(continued)
✓Item Source
 BINDER™ ED 56 Drying and Heating Chamber
•ED056UL-120V Voltage: 120 V 1~60 Hz
•ED056-230V Voltage: 230 V 1~50/60 Hz
 
•BINDER™ 9010-0334
•BINDER™ 9010-0333
 BINDER™ BD 56 Standard-Incubator with natural convection
•BD056UL-120V Voltage: 120 V 1~60 Hz
•BD056-230V Voltage: 230 V 1~50/60 Hz
 
•BINDER™ 9010-0324
•BINDER™ 9010-0323
 Optional—for low throughput of 3 or fewer array plates per week:
•GeneChip™ Hybridization Oven 645[1]
•00-0331
[1] The GeneChip™ Hybridization Oven 640 is currently not supported with the Axiom™ 2.0 Assay. However, to use it in the workflow
contact your field service engineer (FSE) or Thermo Fisher Scientific Technical Support regarding the compatibility of this oven with
the Axiom™ 2.0 Assay.
Spectrophotometer
Unless otherwise indicated, all materials are available through thermofisher.com.
Quantity Item Source
1 Multiskan™ Sky Microplate Spectrophotometer 51119600
Thermal cycler
Unless otherwise indicated, all materials are available through thermofisher.com.
We have verified the performance of this assay using the following o-deck thermal cyclers in their
384-well block configurations:
•ProFlex™ 2×384‑well PCR System (Cat.No.4484077)
•GeneAmp™ PCR System 9700 with dual 384-well sample block module (gold, silver, or aluminum)
The performance of this assay has not been verified with other thermal cyclers. Use of other thermal
cyclers can result in assay failure and may violate the Axiom™ Array and Reagent Replacement policy.
Shakers
Unless otherwise indicated, all materials are available through thermofisher.com.
We recommend using one of the following shakers.
Item Source
Thermo Scientific™ Digital Microplate Shaker 88882005 or 88882006
Thermo Scientific™ Compact Digital Microplate Shaker Fisher Scientific™ 88880023 or 88880024
Jitterbug™Boekel Scientific™: 130000 (115V), 130000-2(230V)
Chapter2Assay equipment and supplies required
Equipment required
2
14 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
Plate centrifuge
One plate centrifuge is required for the Axiom™ 2.0 Assay. The plate centrifuges listed in Table2 are
recommended for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow. When centrifuging
and drying pellets, the centrifuge must be able to centrifuge plates at:
•rcf: 3,200×g with an appropriate rotor/bucket combination (4,000 rpm for the Eppendorf™
Centrifuge 5810 R that is described in Table2)
•Temperature: 4°C
Relative centrifugal force (rcf) is calculated using the following formula:
rcf = (1.118 × 10-5) R S2
where R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolutions per
minute.
In addition, the bottom of the rotor buckets must be soft rubber to ensure that the deepwell plates do
not crack. Do not use buckets where the plates sit directly on a metal or hard plastic bottom. For the
Eppendorf™ Centrifuge 5810 R, do not use the A-4-62 rotor with a WO-15 plate carrier (hard bottom).
Table2Plate centrifuge recommendations for the Axiom™ 2.0 Assay 384HT Array Format Automated
Workflow.
✓Item Source
 Thermo Scientific™ Sorvall™ Legend™ XTR Centrifuge (refrigerated), with: Fisher Scientific™,
75004520 (230 V, 50 Hz)
75004521 (120 V, 60 Hz)
75004523 (230 V, 50–60 Hz,
USA and Canada)
 TX-750 4 + 750mL Swinging Bucket Rotor 75003180 (rotor)
 Microplate/Flask Carriers 75003795 (set of 2 carriers)
75003617 (set of 4 carriers)
Eppendorf™ Centrifuge 5810 R, with:
 Rotor A-4-81, with 4 MTP/Flex buckets
Fisher Scientific™,
022625551 (230 V, 50–60 Hz)
022625501 (120 V, 50–60 Hz, 15 A)
022625101 (120 V, 50–60 Hz, 20 A)
022638807 (rotor)
Allegra™ 25R Benchtop Centrifuge, Refrigerated, with:
 S5700 Swinging-Bucket Rotor
Beckman Coulter™,
369434 (280 V, 60 Hz)
369435 (200 V, 50–60 Hz)
369436 (230 V, 50 Hz)
368954 (rotor)
Chapter2Assay equipment and supplies required
Equipment required 2
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 15
Other equipment required
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
✓Item Supplier Cat.No.
Common laboratory equipment
 Freezer, –20°C MLS —
 Refrigerator, 2°C to 8°C MLS —
 Vortexer MLS —
 Mini microcentrifuge, for 2 mL tubes. MLS —
 Bel-Art Cryo-Safe Mini Cooler, –15°C. Equivalent items from other manufacturers are
acceptable.
MLS —
 Ice bucket, 4 to 9 liters MLS —
Consumables and reagents required for the Axiom™ 2.0
Assay 384HT Array Format Automated Workflow
Applied Biosystems™ software, reagents, arrays, and consumables required
✓Item Cat.No.
Software
 GeneChip™ Command Console™ (GCC) version 6.1 or
later
 Axiom™ Analysis Suite version 5.2 or
later
Reagents
 Axiom™ 2.0 384HT Reagent Kit 902245
(sucient for
one Axiom™
384HT Array
Plate)
Arrays
 Axiom™ 384HT Array Plates
See thermofisher.com for a complete list of array plates available.
Chapter2Assay equipment and supplies required
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow
2
16 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
(continued)
✓Item Cat.No.
Consumables
 Axiom™ 384HT GeneTitan™ High Volume Consumables Kit
Sucient to process 5 Axiom™ 384-format plates. Consists of:
•10 x 384-Layout GeneTitan™ Stain Tray (Stain 1)
•5 x 384-Layout Axiom™ Stain 2 Tray
•5 x 384-Layout Axiom™ Stabilization Tray
•5 x 384-Layout Axiom™ Ligation Tray
•5 x 384-Layout GeneTitan™ Hybridization Tray
•5 x GeneTitan™ Scan Tray
•25 x 384 Layout GeneTitan™ Scan and Stain Tray Cover
These trays are required for processing Axiom™ 384HT Array Plate on the GeneTitan™ Multi-
Channel Instrument. Use the Axiom™ 384HT GeneTitan™ High Volume Consumables Kit
when running the Applied Biosystems™ Axiom™ 2.0 Assay 384HT Array Format Automated
Workflow.
902629
 Axiom™ 384HT Consumables Kit for Biomek™i7‑AB[1, 2]
IMPORTANT! This kit consists of the consumables required for the Axiom™ 2.0 Assay
384HT Array Format Automated Workflow on the Biomek™ i7, method v1.1.
CAUTION! The sample-containing 384 deepwell plate in this consumables kit
requires a special Thermoshake adapter for on-deck incubation steps. Using the
wrong Thermoshake adapter will cause physical interference with the deepwell plate.
CAUTION! Using the wrong type of 96 deepwell plate results in liquid transfer error
such as insucient volume being transferred or dispense tip bottoming out in the
wells. Such liquid transfer errors cause instrument interruption resulting in potential
assay failure.
Sucient for 5 x 384 reaction runs. Consists of:
•10 x 96 PCR plate (Part No. 203208)
•35 x Deep 96 full reservoir (Part No. 203021)
•25 x 96 deepwell 2‑mL plate (Part No. 203016)
•5 x 384 deepwell 250‑μL plate with barcodes (Part No. 203294 or 501624)
•25 x 384 deepwell 250‑μL plate without barcodes (Part No. 203251)
•5 x 384 PCR plate (Part No. 203019)
952472
 Axiom™ 384HT Consumables Kit for QC
Consists of 10 x 384 UV Plate (Part No. 203022)
902289
Chapter2Assay equipment and supplies required
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow 2
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 17
(continued)
✓Item Cat.No.
 Axiom™ 384HT Starter Kit for Biomek™i7‑AB
Consists of:
•1 x Reagent Block Template #1 (Part No. 15-0448)
•1 x Reagent Block Template #2 (Part No. 15-0451)
•1 x Plastic Plate Lid (Part No. 203292)
•1 x Thermoshake 384 Deepwell Plate Adapter (Part No. 203253)
CAUTION! The Thermoshake 384 Adapter is specifically designed to work with
the consumables kit Cat.No.952472. Using the wrong 384 deepwell plate will
result in incompatibility with the adapter.
•1 set Reservoir Stickers (Part No. 90-1111)
•Plate collar (Part No. 203083)
952469
 Axiom™ 384HT Upgrade Kit for Biomek™i7‑AB
•1 x Plastic Plate Lid (Part No. 203292)
•1 x Thermoshake 384 Deepwell Plate Adapter (Part No. 203253)
952471
Training modules available
 Axiom™ 384HT Training Kit for Biomek™i7‑AB
IMPORTANT! This kit consists of the training materials and the consumables required for
the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow on the Biomek™ i7, method
v1.1.
Consists of:
•2 x Axiom™ 2.0 384HT Reagent Kit (Cat. No. 902245)
•1 x Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat. No.952472)
•2 x Axiom™ 384HT GeneTitan™ Consumables Kit (Cat. No. 902234)
•1 x Axiom™ 384HT Consumables Kit for QC (Cat. No. 902289)
•2 x Axiom™ AIMS 1 384S384 Layout Array Plate (Part No. 550413)
•4 x Axiom™ DNA training plates (Part No. 902451)
952473
[1] For quantity required per run, See Table3.
[2] The components of this kit can be ordered directly from the vendor, as listed in Table3.
Chapter2Assay equipment and supplies required
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow
2
18 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
Labware required for the Biomek™ i7 Automated Workstation
The labware that is required to run one array plate the Axiom™ 2.0 Assay 384HT Array Format
Automated Workflow on the Biomek™ i7 Automated Workstation is listed in Table3. Labware images
and ordering information are provided in Table4 on page21.
IMPORTANT! The 384 deepwell plate used in the Axiom™ 2.0 Assay 384HT Array Format Automated
Workflow on the Biomek™ i7, method v1.1, is the Abgene™ AB1178 deepwell plate.
Axiom™ 384HT Consumables Kit for Biomek™i7‑AB
384 deepwell 250‑μL plate (Abgene™ AB1178 deepwell
plate)
IMPORTANT! The 96 deepwell plate used in the Axiom™ 2.0 Assay 384HT Array Format Automated
Workflow on the Biomek™ i7, method v1.1, is the Abgene™ deepwell plate.
Axiom™ 384HT Consumables Kit for Biomek™i7‑AB
96 deepwell 2‑mL plate (Abgene™ deepwell plate)
Table3Labware required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow on
the Biomek™i7.
✓Item Quantity required
Pipette tips, i-Series, 96 tips/rack[1]
 i-Series 50μL filter tips (pink) 768 tips
 i-Series 190μL filter tips (green) 1,618 tips
 i-Series 1,025μL filter tips (orange) 156 tips
Plates (When running target preparation with the Axiom™ 2.0 Assay 384HT Array Format Automated
Workflow on the Biomek™ i7, method v1.1.)
 96 PCR plate[2] 2
 384 PCR plate[2, 3] 1
 96 deepwell 2‑mL plate (Abgene™ deepwell plate)[2] 5
 384 deepwell 250‑μL plate[2, 3] 6
 Deep 96 full reservoir[2] 7
 384 UV plate[3] 1
Chapter2Assay equipment and supplies required
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow 2
Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7 19
Table 3 Labware required for the Axiom 2.0 Assay 384HT Array Format Automated Workflow on the
Biomeki7.(continued)
✓Item Quantity required
Modular reservoirs for reagents, common for method v1.1
 Half Reservoir, 75mL 3
 Quarter Reservoir, 40mL 13
 Quarter Reservoir, Divided by Width, 19mL per
section
1
Other, common for method v1.1
 Frame for Modular Reservoirs 3
 Reagent block 1
 24-Position Tube Rack 1
 Insert, 11 mm, for 1.5-mL Microfuge Tubes (for tube
rack)
2
 Plate Collar 1
 Plastic Plate Lid 1
 384 Deep-Well Adapter 1
 Reagent block templates 1 set
 Reservoir frame stickers 1 set
 Microseal™ 'P' Reusable Replacement PCR Plate
Sealing Pads
as needed
[1] For pipette tips, the “Quantity required” column lists the total number of tips required and not the number of boxes.
[2] Available in the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat. No. 952472).
[3] This plate is also part of the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB. If purchased directly from the supplier, a unique
barcode must be added.
Chapter2Assay equipment and supplies required
Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workflow
2
20 Axiom™ 2.0 Assay 384HT Array Format Automated Workflow Site Preparation Guide—Biomek™i7
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Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

Brand
Thermo Fisher Scientific
Model
Axiom 2.0 Assay 384HT Array Format Automated Workflow
Type
User guide
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