Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

Brand: Thermo Fisher Scientific

Model: Axiom 2.0 Assay 384HT Array Format Automated Workflow

Type: User guide

For Research Use Only. Not for use in diagnostic procedures.

Axiom™ 2.0 Assay 384HT Array Format

Automated Workﬂow

SITE PREPARATION GUIDE

for use with:

Axiom™ 384HT Array Plate

Axiom™ 2.0 384HT Reagent Kit

Biomek™ i7 Automated Workstation

Catalog Number902245

Publication Number MAN0017556

Revision E.0

Thermo Fisher Scientiﬁc Baltics UAB |

V.A. Graiciuno 8, LT-02241 |

Vilnius, Lithuania

Products manufactured at this site:

•Axiom™ 2.0 384HT Reagent Kit

Aymetrix Pte Ltd |

7 Gul Circle #2M-01 |

Keppel Logistics Building |

Singapore 629563

Products manufactured at this site:

•Axiom™ myDesign™ Array Plate

•Axiom™ 384HT Array Plate

Revision history:MAN0017556 E.0 (English)

Revision Date Description

E.0 5 June 2023 •Corrected a part number typo error for a component of the Axiom™ 384HT Consumables Kit for

Biomek™i7‑AB (Cat. No.952472)

•Added an additional part number for a component of the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB

(Cat. No.952472)

•Updated version numbers for required software.

•Updated legacy Aymetrix document numbers with new Thermo Fisher Scientiﬁc publication numbers.

D.0 20 April 2022 •Updated to add information for the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat.No.952472).

•Added an appendix to explain the dierences between the Axiom™ 384HT consumable kits and method

dierences for the Biomek™ i7 Automated Workstation. AppendixA, “Consumable kit and method version

dierences”.

C.0 19 January 2021 Updated to add information for the Axiom™ 384HT Consumables Kit for Biomek™i7 v2 (Cat. No. 952433).

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE

LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR

ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these

products, you accept the terms and conditions of all applicable Limited Use Label Licenses.

TRADEMARKS: All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed. Microsoft,

Windows, Internet Explorer, and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States

and/or other countries. Allegra and Biomek are trademarks of Beckman Coulter, Inc. REPLI-g is a trademark of QIAGEN GmbH. Axygen

is a trademark of Corning Incorporated. Bio-Rad, Hard-Shell, and Microseal are trademarks of Bio-Rad Laboratories, Inc. Zebra is a

trademark of ZIH Corporation. BINDER is a trademark of BINDER GmbH. Boekel Scientiﬁc and Jitterbug are trademarks of Boekel

Scientiﬁc. Thomas Scientiﬁc is a trademark of Thomas Scientiﬁc, LLC. Eppendorf is a registered trademark of Eppendorf AG.

Contents

■CHAPTER1Biomek™ i7 Automated Workstationconﬁguration ................ 4

Biomek™i7components ......................................................... 4

Biomek™i7 conﬁguration andsettings ............................................. 5

Axiom™ 2.0 Assay 384 HT Array Formatmethod .................................... 6

Import themethods ......................................................... 6

Set the method preferences .................................................. 8

Thermoshake plate adapter installation ........................................... 11

Install the Thermoshake plate adapter ........................................ 12

■CHAPTER2Assay equipment and supplies required .......................... 13

Equipment required ............................................................ 13

Applied Biosystems™ equipment required ..................................... 13

Oven requirements ......................................................... 13

Spectrophotometer ........................................................ 14

Thermal cycler ............................................................ 14

Shakers .................................................................. 14

Platecentrifuge ............................................................ 15

Other equipment required ................................................... 16

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array

Format AutomatedWorkﬂow .................................................. 16

Applied Biosystems™ software, reagents, arrays, and consumables required ...... 16

Labware required for the Biomek™ i7 Automated Workstation ................... 19

Templates for Axiom™ 2.0 Assay 384 HT Array Format on the Biomek™i7 ......... 28

Preampliﬁcation/ampliﬁcation staging area .................................... 30

Other reagents and supplies required ........................................ 31

■APPENDIXAConsumable kit and methodversion dierences ............... 32

Consumable kit and method versiondierences ................................... 32

■APPENDIXBDocumentation and support ...................................... 33

Relateddocumentation ......................................................... 33

Customer and technical support ................................................. 35

Limited product warranty ........................................................ 35

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 3

Biomek™ i7 Automated Workstation

conﬁguration

The Beckman Coulter™ Biomek™ i7 Automated Workstation is required to run this version of the

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow with the automated target preparation.

This workstation includes the accessories, software, and deck conﬁguration listed in Table1. In addition

to this workstation, the components that are listed in Chapter 2, “Assay equipment and supplies

required” are also required. This chapter details the Biomek™i7 conﬁguration that is required when

using Windows™ 10 operating system.

Biomek™i7 components

Table1Biomek™ i7 Automated Workstation components

3Item Quantity Beckman Coulter™

Cat.No.

 Biomek™i7 Single-Bridge with Multichannel Pod Automated Liquid

Handler Workstation with Biomek™ Software & Controller 1 B87581

 Multichannel-96 pipetting head, 5-1,200 μL 1 B87589

 1x1 Static ALP Kit 39 B87477

 Trash ALP 1 B87483

 Shaking Peltier ALP 4 A93942

 Vibration Isolation Plate ALP 4 C02750

 1x1 Tip Load Adapter Plate ALP (5 pk) 4 C02751

 Mobile Workstation, i7 1 C02613

4Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

Biomek™i7 conﬁguration and settings

In order for the Axiom™ 2.0 Assay 384 HT Array Format to be successfully performed on the Biomek™i7

Automated Workstation, the system must meet the following speciﬁcations and be properly conﬁgured.

•Biomek™ i7 Automated Workstation

–96MC (model 5, capacity 1,200µL)

–384 Deepwell Adapter (Part No. 203253, available from the starter kit or upgrade kit) installed

on deck location Peltier1

–ALPs fastened to deck as shown in Figure1

Figure1Biomek™i7 deck positions.

•Computer workstation speciﬁcations

–Windows™ 10 operating system

–Biomek Software v5.1.9

•Biomek Software

–Ensure that the i7_Axiom Deck is the default deck

–Ensure deck locations Peltier1, Peltier2, Peltier3, Peltier4 have the device InhecoSingleTEC1,

InhecoSingleTEC2, InhecoSingleTEC3, InhecoSingleTEC4 associated with them.

–Ensure that deck location TR1 is ALP type TrashLeftSide

•Check for a properly framed deck for the Biomek i7 96MC

–Framing tolerance is 0.03 cm. Reframe if this tolerance is exceeded when checked

–If deck was reframed, save a current copy of the instrument ﬁle for backup

–Exit and restart Biomek Software after installing i7_Axiom Deck and after any changes in deck

framing to save the new values in the default instrument (.bif) ﬁle

•Ensure that a −0.65-cm labware oset is found for the BCTubeBlock_2mlTubes labware type on

all four Peltiers. To view and check the oset:

a. From the Utilities tab, click Deck Editor.

b. In the graphical deck representation, double‑click one of the four peltier positions.

c. In the Position Properties window, click More4Per-labware Osets.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Biomek™i7 conﬁguration and settings 1

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 5

d. Select BCTubeBlock_2mlTubes in the left column. Ensure that the Z oset value is set to

−0.65 cm.

e. Click OK twice to return to the graphical deck representation and repeat for each remaining

three peltiers.

Axiom™ 2.0 Assay 384 HT Array Format method

The purpose of this section is to identify critical steps for importing the Axiom™ 2.0 Assay 384 HT Array

Format method into the Biomek™ Software for use on the Biomek™ i7 Automated Workstation.

Import the methods

1. Copy the following folders from method package on the USB drive and transfer them to

C:\Users\Public\Documents.

•Axiom 2.0 384

•Axiom 2.0 Methods_v1.1

2. If the Biomek Software is open, close it before proceeding to ensure that internal data tables are in

their default state.

3. Open the Biomek™ Software using one of the following methods:

•Double‑click the Biomek icon on the desktop.

•In the Windows Search box, enter Biomek 5.

4. Click File4New4Project, then name the project Axiom 384 on i7.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Axiom™ 2.0 Assay 384 HT Array Format method

6Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

5. Ensure that the Axiom 384 on i7 project is currently open by checking the project name

that is displayed in the bottom left of the Biomek™ Software window. If it is not open, click

File4Open4Project, then select Axiom 384 on i7.

6. Click File4Import.

7. In the Import Method window, navigate to C:/Users/Public/Documents/TFS/Axiom 2.0

384 Methods_v1.1 folder containing the six methods.

8. Select a method to import, then click OK. If this method is the ﬁrst being imported, click OK on the

Import Project prompt to update the project.

9. Repeat step8 until all six methods have been imported.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Axiom™ 2.0 Assay 384 HT Array Format method 1

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 7

Set the method preferences

IMPORTANT! The method preferences must be selected before starting the method. These settings

are not prompted for at runtime. Typically, the Thermo Fisher Scientiﬁc ﬁeld application scientist (FAS)

sets the method preferences during method installation.

1. Open the Biomek™ Software using one of the following methods:

•Double‑click the Biomek 5 icon on the desktop.

•In the Windows Search box, enter Biomek 5.

2. Click File4Open.

3. For Look in of the Open Method window, select Axiom 384 on i7.

4. Click to select a method, then click OK.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Axiom™ 2.0 Assay 384 HT Array Format method

8Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

5. In the Method View panel, click the line that reads Click Here to Select Method Preferences

(Figure2).

Figure2Click to select method preferences.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Axiom™ 2.0 Assay 384 HT Array Format method 1

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 9

6. Click to select the method preferences.

1Method v1.1 information.

Logging options.

•Barcode Tracking: This feature is enabled by default. It is highly recommended to use the

barcode tracking feature to ensure accuracy in processing the samples. Consult with a FAS

if it is necessary to disable the barcode tracking feature. When selected the barcode tracking

feature performs the following actions:

–Prompts you to enter the barcode information on sample plates, Axiom™ reagent

modules, and GeneTitan™ consumables.

–Records barcode information into a log ﬁle.

–Ensures that the correct ampliﬁcation time for the samples has elapsed (minimum of 22

hours) before starting the fragmentation and precipitation step.

–Ensures that the splitting and reassembly of samples are correctly associated to the

starting sample plate.

Note: If using custom barcodes for plates, only use alpha-numeric, hyphen, or underscore

characters.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Axiom™ 2.0 Assay 384 HT Array Format method

10 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

Note: The barcode tracking feature is intended to track samples that go through the entire

target preparation process on one instrument.

•Log Name: The log name is the name of the barcode log ﬁle. The default name is

BarcodeLog[InstSN].txt, where "InstSN" is the serial number of the instrument. Naming of

the log ﬁle is possible by selecting Other in the dropdown list for the setting. If using a custom

log name, ensure that the name ends with ".txt" otherwise the ﬁle type will not be associated

correctly. After entering a custom name, click the Apply to the right of the log name ﬁeld.

•Log Path: The log path is the barcode log ﬁle location. The log path is:

C:\Users\Public\Documents\Axiom 2.0 384\

Custom run options.

•Verify Reagent Module barcodes: When selected this feature ensures that the correct

Axiom™ reagent is used in the speciﬁed target preparation step.

•Verify GeneTitan Labware Setup: When selected, this feature ensures that the GeneTitan™

reagents that are prepared on the Biomek™i7 are correctly associated with the GeneTitan™

plate type.

•Prepare gel and OD plates for QC: the workstation prepares two plates of resuspended

samples that are properly diluted for the fragmentation gel QC and OD quantiﬁcation process

control checks.

IMPORTANT! For troubleshooting and support purposes, we strongly recommend that you

perform the gel QC and OD quantiﬁcation process controls after resuspension.

•Test Mode: Select this option to skip all the incubation timers in a step. If selected, a prompt

is displayed asking you to ensure that you want to run a step in test mode. Use this option to

perform runs using deionized water only, not actual reagents or samples.

CAUTION! Never process samples in test mode. Processing samples in test mode results

in assay failure and loss of samples and reagents.

Note: Perform veriﬁcation tasks listed on the Axiom™ 384 on i7 System Readiness Checklist.

Thermoshake plate adapter installation

The Thermoshake 384 deepwell plate adapter that is used with the Axiom™ 2.0 Assay 384HT Array

Format Automated Workﬂow method is designed speciﬁcally to ﬁt the 384 deepwell 250‑μL plate.

Proper installation is critical to ensuring even heating of the samples and proper liquid transfer in the

fragmentation step.

The Thermoshake 384 Deepwell Plate Adapter is a component of the Axiom™ 384HT Starter Kit for

Biomek™i7‑AB (Cat. No. 952469) or the Axiom™ 384HT Upgrade Kit for Biomek™i7‑AB (Cat. No.

952471).

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Thermoshake plate adapter installation 1

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 11

Install the Thermoshake plate adapter

1. Ensure that the 2 notched corners are oriented facing the front of the Thermoshake 384 Deepwell

Plate Adapter.

Figure3Thermoshake 384 Deepwell Plate Adapter orientation on the Biomek™i7 deck.

2. Press down ﬁrmly to ensure that adapter is evenly seated on the shaker and that the bottom

surface of the Thermoshake adapter is ﬂush on the top surface of the shaker.

Chapter1Biomek™ i7 Automated Workstation conﬁguration

Thermoshake plate adapter installation

12 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

Assay equipment and supplies

required

This chapter includes the supplier and ordering information for the equipment, software, reagents,

arrays, labware, and other consumables that have been veriﬁed for use with the Applied Biosystems™

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow.

Equipment required

Applied Biosystems™ equipment required

✓Item Cat.No.

 GeneTitan™ Multi-Channel Instrument[1] Contact Thermo Fisher Scientiﬁc

 Digital Scanner/Barcode Reader (to enable barcode tracking)

External Barcode Reader Holder (to enable barcode tracking)

74-0016

00-0093

[1] For a complete list of all equipment and supplies required for GeneTitan™ Multi-Channel Instrument installation and operation, consult

the GeneTitan™ Multi-Channel Instrument Site Preparation Guide (Pub. No. MAN0025571)

Oven requirements

We recommend using either the Thermo Scientiﬁc™ Heratherm™ Advanced Protocol Microbiological

Incubator, BINDER™ ED 56 Drying and Heating Chamber, or the BINDER™ BD 56 Standard-Incubator

with natural convection that are listed in the following table. If another oven is used, it must meet the

following requirements.

•Be able to maintain a constant temperature of 37°C for at least 24 hours, and have a temperature

accuracy of ±1°C, and

•Be able to maintain a constant temperature of 48°C for at least 24 hours, and have a temperature

accuracy of ±1°C.

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

✓Item Source

 Thermo Scientiﬁc™ Heratherm™ Advanced Protocol Microbiological

Incubator, capacity 66 L

•120V, 60 Hz

•230V, 50/60 Hz

  

•51028066

•51028133

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 13

(continued)

✓Item Source

 BINDER™ ED 56 Drying and Heating Chamber

•ED056UL-120V Voltage: 120 V 1~60 Hz

•ED056-230V Voltage: 230 V 1~50/60 Hz

 

•BINDER™ 9010-0334

•BINDER™ 9010-0333

 BINDER™ BD 56 Standard-Incubator with natural convection

•BD056UL-120V Voltage: 120 V 1~60 Hz

•BD056-230V Voltage: 230 V 1~50/60 Hz

 

•BINDER™ 9010-0324

•BINDER™ 9010-0323

 Optional—for low throughput of 3 or fewer array plates per week:

•GeneChip™ Hybridization Oven 645[1]

•00-0331

[1] The GeneChip™ Hybridization Oven 640 is currently not supported with the Axiom™ 2.0 Assay. However, to use it in the workflow

contact your field service engineer (FSE) or Thermo Fisher Scientific Technical Support regarding the compatibility of this oven with

the Axiom™ 2.0 Assay.

Spectrophotometer

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Quantity Item Source

1 Multiskan™ Sky Microplate Spectrophotometer 51119600

Thermal cycler

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

We have veriﬁed the performance of this assay using the following o-deck thermal cyclers in their

384-well block conﬁgurations:

•ProFlex™ 2×384‑well PCR System (Cat.No.4484077)

•GeneAmp™ PCR System 9700 with dual 384-well sample block module (gold, silver, or aluminum)

The performance of this assay has not been veriﬁed with other thermal cyclers. Use of other thermal

cyclers can result in assay failure and may violate the Axiom™ Array and Reagent Replacement policy.

Shakers

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

We recommend using one of the following shakers.

Item Source

Thermo Scientiﬁc™ Digital Microplate Shaker 88882005 or 88882006

Thermo Scientiﬁc™ Compact Digital Microplate Shaker Fisher Scientiﬁc™ 88880023 or 88880024

Jitterbug™Boekel Scientiﬁc™: 130000 (115V), 130000-2(230V)

Chapter2Assay equipment and supplies required

Equipment required

14 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

Plate centrifuge

One plate centrifuge is required for the Axiom™ 2.0 Assay. The plate centrifuges listed in Table2 are

recommended for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow. When centrifuging

and drying pellets, the centrifuge must be able to centrifuge plates at:

•rcf: 3,200×g with an appropriate rotor/bucket combination (4,000 rpm for the Eppendorf™

Centrifuge 5810 R that is described in Table2)

•Temperature: 4°C

Relative centrifugal force (rcf) is calculated using the following formula:

rcf = (1.118 × 10-5) R S2

where R is the radius of the rotor in centimeters, and S is the speed of the centrifuge in revolutions per

minute.

In addition, the bottom of the rotor buckets must be soft rubber to ensure that the deepwell plates do

not crack. Do not use buckets where the plates sit directly on a metal or hard plastic bottom. For the

Eppendorf™ Centrifuge 5810 R, do not use the A-4-62 rotor with a WO-15 plate carrier (hard bottom).

Table2Plate centrifuge recommendations for the Axiom™ 2.0 Assay 384HT Array Format Automated

Workﬂow.

✓Item Source

 Thermo Scientiﬁc™ Sorvall™ Legend™ XTR Centrifuge (refrigerated), with: Fisher Scientiﬁc™,

75004520 (230 V, 50 Hz)

75004521 (120 V, 60 Hz)

75004523 (230 V, 50–60 Hz,

USA and Canada)

 TX-750 4 + 750mL Swinging Bucket Rotor 75003180 (rotor)

 Microplate/Flask Carriers 75003795 (set of 2 carriers)

75003617 (set of 4 carriers)

Eppendorf™ Centrifuge 5810 R, with:

 Rotor A-4-81, with 4 MTP/Flex buckets

Fisher Scientiﬁc™,

022625551 (230 V, 50–60 Hz)

022625501 (120 V, 50–60 Hz, 15 A)

022625101 (120 V, 50–60 Hz, 20 A)

022638807 (rotor)

Allegra™ 25R Benchtop Centrifuge, Refrigerated, with:

 S5700 Swinging-Bucket Rotor

Beckman Coulter™,

369434 (280 V, 60 Hz)

369435 (200 V, 50–60 Hz)

369436 (230 V, 50 Hz)

368954 (rotor)

Chapter2Assay equipment and supplies required

Equipment required 2

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 15

Other equipment required

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that

the material is available from ﬁsherscientiﬁc.com or another major laboratory supplier.

✓Item Supplier Cat.No.

Common laboratory equipment

 Freezer, –20°C MLS —

 Refrigerator, 2°C to 8°C MLS —

 Vortexer MLS —

 Mini microcentrifuge, for 2 mL tubes. MLS —

 Bel-Art Cryo-Safe Mini Cooler, –15°C. Equivalent items from other manufacturers are

acceptable.

MLS —

 Ice bucket, 4 to 9 liters MLS —

Consumables and reagents required for the Axiom™ 2.0

Assay 384HT Array Format Automated Workﬂow

Applied Biosystems™ software, reagents, arrays, and consumables required

✓Item Cat.No.

Software

 GeneChip™ Command Console™ (GCC) version 6.1 or

later

 Axiom™ Analysis Suite version 5.2 or

later

Reagents

 Axiom™ 2.0 384HT Reagent Kit 902245

(sucient for

one Axiom™

384HT Array

Plate)

Arrays

 Axiom™ 384HT Array Plates

See thermoﬁsher.com for a complete list of array plates available.



Chapter2Assay equipment and supplies required

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow

16 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

(continued)

✓Item Cat.No.

Consumables

 Axiom™ 384HT GeneTitan™ High Volume Consumables Kit

Sucient to process 5 Axiom™ 384-format plates. Consists of:

•10 x 384-Layout GeneTitan™ Stain Tray (Stain 1)

•5 x 384-Layout Axiom™ Stain 2 Tray

•5 x 384-Layout Axiom™ Stabilization Tray

•5 x 384-Layout Axiom™ Ligation Tray

•5 x 384-Layout GeneTitan™ Hybridization Tray

•5 x GeneTitan™ Scan Tray

•25 x 384 Layout GeneTitan™ Scan and Stain Tray Cover

These trays are required for processing Axiom™ 384HT Array Plate on the GeneTitan™ Multi-

Channel Instrument. Use the Axiom™ 384HT GeneTitan™ High Volume Consumables Kit

when running the Applied Biosystems™ Axiom™ 2.0 Assay 384HT Array Format Automated

Workﬂow.

902629

 Axiom™ 384HT Consumables Kit for Biomek™i7‑AB[1, 2]

IMPORTANT! This kit consists of the consumables required for the Axiom™ 2.0 Assay

384HT Array Format Automated Workﬂow on the Biomek™ i7, method v1.1.

CAUTION! The sample-containing 384 deepwell plate in this consumables kit

requires a special Thermoshake adapter for on-deck incubation steps. Using the

wrong Thermoshake adapter will cause physical interference with the deepwell plate.

CAUTION! Using the wrong type of 96 deepwell plate results in liquid transfer error

such as insucient volume being transferred or dispense tip bottoming out in the

wells. Such liquid transfer errors cause instrument interruption resulting in potential

assay failure.

Sucient for 5 x 384 reaction runs. Consists of:

•10 x 96 PCR plate (Part No. 203208)

•35 x Deep 96 full reservoir (Part No. 203021)

•25 x 96 deepwell 2‑mL plate (Part No. 203016)

•5 x 384 deepwell 250‑μL plate with barcodes (Part No. 203294 or 501624)

•25 x 384 deepwell 250‑μL plate without barcodes (Part No. 203251)

•5 x 384 PCR plate (Part No. 203019)

952472

 Axiom™ 384HT Consumables Kit for QC

Consists of 10 x 384 UV Plate (Part No. 203022)

902289

Chapter2Assay equipment and supplies required

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow 2

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 17

(continued)

✓Item Cat.No.

 Axiom™ 384HT Starter Kit for Biomek™i7‑AB

Consists of:

•1 x Reagent Block Template #1 (Part No. 15-0448)

•1 x Reagent Block Template #2 (Part No. 15-0451)

•1 x Plastic Plate Lid (Part No. 203292)

•1 x Thermoshake 384 Deepwell Plate Adapter (Part No. 203253)

CAUTION! The Thermoshake 384 Adapter is speciﬁcally designed to work with

the consumables kit Cat.No.952472. Using the wrong 384 deepwell plate will

result in incompatibility with the adapter.

•1 set Reservoir Stickers (Part No. 90-1111)

•Plate collar (Part No. 203083)

952469

 Axiom™ 384HT Upgrade Kit for Biomek™i7‑AB

•1 x Plastic Plate Lid (Part No. 203292)

•1 x Thermoshake 384 Deepwell Plate Adapter (Part No. 203253)

952471

Training modules available

 Axiom™ 384HT Training Kit for Biomek™i7‑AB

IMPORTANT! This kit consists of the training materials and the consumables required for

the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow on the Biomek™ i7, method

v1.1.

Consists of:

•2 x Axiom™ 2.0 384HT Reagent Kit (Cat. No. 902245)

•1 x Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat. No.952472)

•2 x Axiom™ 384HT GeneTitan™ Consumables Kit (Cat. No. 902234)

•1 x Axiom™ 384HT Consumables Kit for QC (Cat. No. 902289)

•2 x Axiom™ AIMS 1 384S384 Layout Array Plate (Part No. 550413)

•4 x Axiom™ DNA training plates (Part No. 902451)

952473

[1] For quantity required per run, See Table3.

[2] The components of this kit can be ordered directly from the vendor, as listed in Table3.

Chapter2Assay equipment and supplies required

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow

18 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

Labware required for the Biomek™ i7 Automated Workstation

The labware that is required to run one array plate the Axiom™ 2.0 Assay 384HT Array Format

Automated Workﬂow on the Biomek™ i7 Automated Workstation is listed in Table3. Labware images

and ordering information are provided in Table4 on page21.

IMPORTANT! The 384 deepwell plate used in the Axiom™ 2.0 Assay 384HT Array Format Automated

Workﬂow on the Biomek™ i7, method v1.1, is the Abgene™ AB1178 deepwell plate.

Axiom™ 384HT Consumables Kit for Biomek™i7‑AB

384 deepwell 250‑μL plate (Abgene™ AB1178 deepwell

plate)

IMPORTANT! The 96 deepwell plate used in the Axiom™ 2.0 Assay 384HT Array Format Automated

Workﬂow on the Biomek™ i7, method v1.1, is the Abgene™ deepwell plate.

Axiom™ 384HT Consumables Kit for Biomek™i7‑AB

96 deepwell 2‑mL plate (Abgene™ deepwell plate)

Table3Labware required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow on

the Biomek™i7.

✓Item Quantity required

Pipette tips, i-Series, 96 tips/rack[1]

 i-Series 50μL ﬁlter tips (pink) 768 tips

 i-Series 190μL ﬁlter tips (green) 1,618 tips

 i-Series 1,025μL ﬁlter tips (orange) 156 tips

Plates (When running target preparation with the Axiom™ 2.0 Assay 384HT Array Format Automated

Workﬂow on the Biomek™ i7, method v1.1.)

 96 PCR plate[2] 2

 384 PCR plate[2, 3] 1

 96 deepwell 2‑mL plate (Abgene™ deepwell plate)[2] 5

 384 deepwell 250‑μL plate[2, 3] 6

 Deep 96 full reservoir[2] 7

 384 UV plate[3] 1

Chapter2Assay equipment and supplies required

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow 2

Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7 19

Table 3 Labware required for the Axiom 2.0 Assay 384HT Array Format Automated Workﬂow on the

Biomeki7.(continued)

✓Item Quantity required

Modular reservoirs for reagents, common for method v1.1

 Half Reservoir, 75mL 3

 Quarter Reservoir, 40mL 13

 Quarter Reservoir, Divided by Width, 19mL per

section

Other, common for method v1.1

 Frame for Modular Reservoirs 3

 Reagent block 1

 24-Position Tube Rack 1

 Insert, 11 mm, for 1.5-mL Microfuge Tubes (for tube

rack)

 Plate Collar 1

 Plastic Plate Lid 1

 384 Deep-Well Adapter 1

 Reagent block templates 1 set

 Reservoir frame stickers 1 set

 Microseal™ 'P' Reusable Replacement PCR Plate

Sealing Pads

as needed

[1] For pipette tips, the “Quantity required” column lists the total number of tips required and not the number of boxes.

[2] Available in the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB (Cat. No. 952472).

[3] This plate is also part of the Axiom™ 384HT Consumables Kit for Biomek™i7‑AB. If purchased directly from the supplier, a unique

barcode must be added.

Chapter2Assay equipment and supplies required

Consumables and reagents required for the Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow

20 Axiom™ 2.0 Assay 384HT Array Format Automated Workﬂow Site Preparation Guide—Biomek™i7

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Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

Thermo Fisher Scientific Axiom 2.0 Assay 384HT Array Format Automated Workflow User guide

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