The tecan GENESIS FE500 is an easy-to-use, yet fully automated workstation designed for clinical diagnostic laboratories. With its ability to perform a wide range of molecular biology applications, including nucleic acid extraction, PCR setup, and real-time PCR, the FE500 helps streamline laboratory workflows and improve efficiency. Featuring a user-friendly interface, the FE500 offers customizable protocols, barcode reading capabilities, and flexible sample handling options, making it ideal for laboratories of all sizes.
The tecan GENESIS FE500 is an easy-to-use, yet fully automated workstation designed for clinical diagnostic laboratories. With its ability to perform a wide range of molecular biology applications, including nucleic acid extraction, PCR setup, and real-time PCR, the FE500 helps streamline laboratory workflows and improve efficiency. Featuring a user-friendly interface, the FE500 offers customizable protocols, barcode reading capabilities, and flexible sample handling options, making it ideal for laboratories of all sizes.
)(
QUICK GUIDE
NGLISH
Safety Instructions page 3
Operation - Daily Routines page 7
Preliminary Work page 7
Starting page 7
Sample Loading page 8
Sample Unloading page 10
Shut Down page 11
Maintenance page 11
Retrieve Information page 17
Refilling and Disposal page 21
Error Handling page 29
Index page 32
Doc. No. 391 401
Version 1.0
June 2002
2
Document History Doc ID Version Description Issued
391 401 V1.0 First issue June 2002
The information contained in this document is subject to change without notice.
© 2002 Tecan Schweiz AG, Switzerland, all rights reserved.
)( Quick Guide Introduction
3
Safety Instructions
This chapter contains general information assuring safe opera-
tion of the instrument. More safety instructions are given
throughout this Guide, at the respective locations where obser-
vation is most important.
Strictly follow all safety instructions in this publication.
Introduction
TECAN products are designed to operate safely when installed
and used according to good laboratory practice guidelines. The
instructions included in this section explain the potential risks
associated with instrument operation and provide important
safety practices designed to minimize these risks.
If you carefully follow the information contained in this section,
you can protect yourself from hazards and create a safer work
environment.
Definition
Operator: Qualified person who uses the
equipment for its intended purpose.
Field Service Engineer: Authorized person responsible for
installation, initial start up of the in-
strument and Operator’s training on-
site.
Person to carry out service tasks.
Target Group
The )( has been conceived for applications in the
clinical diagnostic market, requiring qualified and authorized
laboratory personnel.
Instrument operation requires thorough knowledge of applica-
tions, instrument functions and software programs as well as all
applicable safety rules and regulations.
Qualification, Training
To be qualified for instrument operation, you must read and un-
derstand the written instructions in the operating manual (p/n
391 400), have appropriate training (by TECAN Field Service
Engineer), and know the safety rules and regulations.
Unintended Use
The )( is not recommended for repetitive serial dilu-
tions. Accuracy and reproducibility are NOT sufficient for this
purpose.
)( Quick Guide Warning Notices
4
Warning Notices
On the instrument and throughout this publication, warning no-
tices are to be interpreted as follows:
Safety - Basics
General Operating Hazards
For all applications of the TECAN instrument, it is essential that
the user strictly follows all instructions described in the related
manuals.
The TECAN instrument requires accurate positioning of all car-
riers, racks and buckets on the instrument’s worktable. The op-
erator should verify this before switching on the instrument.
In the event of power failure or an otherwise aborted run, the in-
formation of all partially processed samples will be lost. There-
fore, an Uninterrupted Power Supply is highly recommen-
ded!
Upon attempting to restart an interrupted program, the comput-
er screen provides instructions for resuming operation.
All precautions must be followed to ensure safe operation of the
instrument.
WARNING
Indicates the possibility of severe personal in-
jury, loss of life or equipment damage if in-
structions are not followed.
The Icon Type indicates which kind of risk a person or
equipment is exposed to.
Refer to instructions
Electrostatic discharge sensitive de-
vices
Laser radiation
Biological hazard
Pinch point
CAUTION
Indicates the possibility of severe equipment
damage if instructions are not followed.
NOTE
Gives helpful information about the equipment.
)( Quick Guide General Safety Regulations
5
Mechanical Hazards
Keep the housing and safety panels closed and never reach
into the instrument work space when the unit is in operating
mode.
Chemical and Biological Hazards
All samples must be considered as potentially infectious. Han-
dle all specimens using established good laboratory working
practices and strictly apply appropriate safety precautions ac-
cording to local, state and federal regulations.
After redefining the routing, test runs shall be made with a neu-
tral liquid to allow optimization of all liquid handling parameters
and the routing.
Prior to executing any preventive maintenance task on the in-
strument or returning parts for repair, the instrument and/or the
parts must be thoroughly decontaminated.
Waste
Handling and disposing of waste has to be in accordance with
all local, state and federal environmental, health, safety laws
and regulations.
Operating Manual Location
The )(Operator’s Manual, p/n 391 400 shall be kept
in proximity of the )(.
The Operator’s Manual is also located in the )( directory in
a PDF file format.
General Safety Regulations
Legal Requirements
The user is responsible for correctly operating the
)(, according to all local, state and federal laws that may
apply. All precautions must be followed to ensure safe opera-
tion of the instrument.
General Inspection and Maintenance Duties
The TECAN instrument shall only be operated in optimal run-
ning condition. The preventive maintenance procedures listed
in the )( Operator’s Manual, p/n 391 400 must be
performed.
Only authorized Field Service Engineers shall carry out PC
configuration, installation and service tasks.
In case of safety relevant deficiencies, TECAN must immedi-
ately be informed.
Spare Parts and Disposables
Use only official TECAN spare parts and disposables. If other
parts are used during the normal warranty period, the manufac-
turer’s warranty is voided.
)( Quick Guide FCC Declaration
6
Modifications
Modifications and instrument upgrades shall only be carried out
by an authorized Field Service Engineer. TECAN will not accept
responsibility for any claim resulting from unauthorized modifi-
cation or alteration.
Barcode Readers
Barcode Readers are used in the following modules: Tube
Loading Unit, Aliquoter, Conveyor (Puck Barcode Readers and
Tube Barcode Readers).
Functional Notes, Precautions
Cell Phones and Pagers
FCC Declaration
This equipment has been tested and found to comply with the
limits for a Class A digital device, pursuant to both Part 15 of
the rules and the radio interference regulations of the Canadian
Department of Communication (FCC). These limits are de-
signed to provide reasonable protection against harmful inter-
ference when equipment is operated in a commercial
environment. This equipment generates, uses and can radiate
radio frequency energy and may cause harmful interferences to
radio communications, if not installed and used in accordance
with the instruction manual. Operation of this equipment in a
residential area is likely to cause harmful interferences. In
which case, the user will be required to correct the interferenc-
es at his expense.
WARNING
Laser radiation
Do not stare into the beam. Class 2 Laser
product.
NOTE
To ensure proper operation, laser beam output
windows must be clean at all times. Even slight soiling
will cause reading errors.
CAUTION
Switch off Cell Phones and Pagers in this room.
The use of Cell Phones leads to faulty liquid
detection results.
)( Quick Guide Preliminary Work
7
Operation - Daily Routines
For detailed instructions, refer to the )( Operator’s
Manual, p/n 391 400.
Safety Instructions
The Safety Instructions must strictly be followed. Refer to
Chapter: Safety Instructions on page 3 and to the special in-
structions in the cor
responding chapters in the
)(
Operator’s Manual, p/n 391 400.
Preliminary Work
Preliminary Checks
• Ensure that the Daily Preventive Maintenance upon shut-
ting down the System is done.
• Ensure that all Containers for Disposables are refilled and
all wastes are emptied. Refer to the corresponding des-
cription in the Chapter: Refilling and Disposal on page 21.
• Make sure four Centrifuge Buckets are placed on the
Worktable, correctly oriented. Check also the Buckets in
the Centrifuge.
• Check the orientation of the Buckets on the Worktable and
in the Centrifuge.
• Ensure that all doors, the drawers and the front Panel are
closed.
Starting
1 If necessary, refill DiTi, Secondary Tubes, Labels, and
empty the Wastes.
2 Switch on the power on the right side of the instrument n.
3 Start the Application Software. After starting, the instru-
ment will initialize.
CAUTION
• Turn-off all cell phones and pagers.
• Before starting, make sure that the Daily Main-
tenance has been carried out properly. Refer to
the )(Operator’s Manual, p/n 391 400.
• After switching off the instrument, wait a mini-
mum of 10 seconds before starting it again.
1
)( Quick Guide Sample Loading
8
4 In the main window, click the 6WDUW button o. During
start up, the button changes from green to orange. When
the start procedure is completed, the button changes to
red. This indicates that access to the inside of the instru-
ment could be dangerous and it highlights the button for
system stop.
Sample Loading
Inserting Sample Tubes into the Loading Racks
2
WARNING: Potential Biohazard!
• Consider all clinical specimens, surfaces
or components that contain or have con-
tacted human blood or serum as potential
-
ly infectious. Wear gloves, lab coats, safety
glasses, and follow biosafety practices.
• Handle the Tray with the Racks carefully.
CAUTION
• Do not centrifuge any Primary Tubes without
Caps.
• Observe the correct Barcode Label orientation
of the Primary Tubes.
)( Quick Guide Sample Loading
9
Loading Racks
1 Load Racks onto tray.
2 Insert tray n.
3 Carefully push the Racks onto the Tube Loading Unit
o.
The Racks are moved automatically to the rear.
4 Remove the empty tray
p.
Removing Empty Loading Racks
5 Regularly remove the empty Racks q. When space is no
longer available, a warning message is displayed asking
for Racks to be removed.
CAUTION
• Always remove the trays after loading or un-
loading to prevent Racks from falling out of the
tray.
2
3
1
4
CAUTION
• Always remove the Trays after loading or un-
loading to prevent Racks from falling out of the
Tray.
NOTE
Rejected Tubes remain in the Loading Racks.
)( Quick Guide Sample Unloading
10
Sample Unloading
L
1 Push one of the buttons in front of the Carrier to be un-
loaded
n. Reset an erroneously pressed button by briefly
pressing it again.
2 When the red LED is flashing and the green LED is
dark, pull the Carrier carefully towards the front and re-
move it from the Worktable
o.
3 Place a Carrier with empty Racks onto the )(
Worktable. Observe the color strips on the Carrier front
and the Front Safety Panel
p. Both LEDs are flashing
alternatively.
4 Push one of the interface buttons in front of the re-
placed Carrier until the red LED stops flashing.
WARNING: Potential Biohazard!
• Consider all clinical specimens, surfaces
or components that contain or have con-
tacted human blood or serum as potential-
ly
infectious. Wear gloves, lab coats, safety
glasses, and follow biosafety practices.
WARNING: Risk of Contamination!
• Use caution handling the Carriers to avoid
sample splashing.
CAUTION
If the green LED is lit: Do not try to pull out the Car-
rier.
1
2
3
CAUTION
Do not remove the Carrier as long as the green LED
is lit.
CAUTION
To re-insert a not emptied Carrier:
Make sure the specimens are in their original spots
to avoid crashes and push the interface button
briefly.
)( Quick Guide Shut Down
11
Shut Down
Refer to Maintenance Check List in the Operator’s Manual.
Refer
1 In the main window, click the 6WDUW button
n. Wait until
the button has changed from red to orange to green.
2 In the )LOH menu, select ([LW
o. Wait until the Appli-
cation has closed.
3 Switch off the power
p.
4 Shut down the PC.
Maintenance
Syringe and Diluter Valve
1 Check and tighten Syringe in valve n.
2 Check inside of Syringe Plunger below PTFE cap o. If
wet, replace the syringe.
3 Check the Plunger lock screw p and tighten, if necessary.
2
3
1
1
2
3
4
)( Quick Guide Maintenance
12
Diluter Valve
4 Bubbles in the Tubes q after flushing may be caused by
untightened Syringe, untightened Plunger lock screw, lack
of System Liquid, aspirating air in the Tube, or a leaking
Diluter Valve. Replace the Diluter Valve, if necessary.
5
DiTi Cone
1 Clean the DiTi Cone n with weak detergent and disinfect
with alcohol.
2 Tighten using the Cone Wrench (Part No 619 517), if nec-
essary o.
DiTi Waste
Check DiTi Waste Bag and replace if necessary.
Printer
1 Check for lost Labels n.
2 Check and clean the Pressure Rollers o.
1
2
1
2
)( Quick Guide Maintenance
13
Pick and Place Grippers
Cleaning
1 Clean and disinfect the Gripper fingers n using a lint-free
tissue.
Unload Area and Unload Interface
1 Clean and disinfect Unload Area, Carriers, and Racks n.
2 Wipe down the Unload Interface o.
CAUTION
• Do not use detergent sprays to prevent mois-
ture from getting into the electronic system or
degreasing moving mechanical parts.
• Do not spray the panels. Use a moist, lint-free
tissue.
1
CAUTION
Do not spray desinfectant or cleaner on the LED’s.
1
2
)( Quick Guide Maintenance
14
Chuck and Tube Gripper
1 Clean the Chuck n and the Tube Gripper Jaw o with a
weak detergent, and disinfect using alcohol.
2 Check the inner surfaces for sign of wear.
Cap Waste
Check Cap Waste Bag and replace if necessary.
Compartment of the Waste Station
1. If the Aliquoter arm is above the Waste Station, move it to
the side.
2. Carefully, remove the compartment
n.
3. Clean and disinfect.
4 Reinsert the compartment o
CAUTION
• Do not use detergent sprays to prevent mois-
ture from getting into the electronic system or
degreasing moving mechanical parts.
• Do not spray the panels. Use a moist, lint free
tissue.
1
2
WARNING: Potential Biohazard!
Wear gloves, lab coats, safety
glasses, and fol-
low biosafety practices.
2
1
)( Quick Guide Maintenance
15
Splash Protection Shields
1. Remove the two screws of the inner Splash Protection
shield
n.
2. Carefully, remove the shield
o.
3. Clean and disinfect.
4. Loosen the two screws of the outer Splash Protection
shield
p.
5. Carefully, remove the shield q.
6. Clean and disinfect.
7. Carefully, mount the outer Splash Protection shield r.
8. Tighten the two screws
p.
9. Carefully, mount the inner Splash Protection shield s.
10. Tighten the two screws n.
11. Carefully, slide the inner Splash Protection shield up and
down to check for collision with the Sensors
t.
2
3
4
5
6
8
1
8
7
CAUTION
In case of collision with the Sensors, slightly bend
the upper and/or lower tongue u of the inner
Splash Protection shield.
)( Quick Guide Maintenance
16
Arm Guide Rails
Clean the Arm Guide Rails n with a lint-free tissue.
Centrifuge
Clean and grease the bearing surfaces of the hangers. Consult
Operator’s Manual if necessary.
1
)( Quick Guide Retrieve Information
17
Retrieve Information
Displaying Sample Information
1 In the Unload Area of the Worktable, double-click the relat-
ed tube in order to access the sample info window.
n Tube Barcode
o Volume in the tube
p Primary or Secondary Tube
q Puck Barcode
r Position information (Carrier, Rack, Sample)
s Status of the tube
t &ORVH button
u Current Step
v Next Step
w History table with the executed actions and error status.
Displaying Routing Information
n Refer to Orders on page 18.
o Refer to Status of Tube on page 18.
p Refer to Hierarchy of Orders on page 19.
q Refer to Information window for highlighted line in Hierarchy
section on page 19.
r Refer to Menu and Tool Bar Functions on page 20.
1
2
3
4
5
6
7
8
9
10
2
4
5
1
3
)( Quick Guide Retrieve Information
18
Orders
Status of system:
Total number of Orders received from the
Laboratory Information System.
Number of Orders the Primary Tube has
yet to load.
Number of Primary Tubes with unsuccess-
ful Order Request from the Laboratory In-
formation System.
Number of Orders that have been suc-
cessfully processed.
Number of Orders with at least one tube
that had an error during processing.
Status of Tube
Successfully
completed
(Tube is re-
moved from
)()
Date and
time tube
was re-
ceived on
the system
Status of tube/order
In process
Error
(tube may or
may not be
on )()
Refer to
‘Status’
)( Quick Guide Retrieve Information
19
Hierarchy of Orders
1 In the Status table, select the order.
Information window for highlighted line in Hierarchy
section
Highlight the order, Primary Tube or Secondary Tube for addi-
tional information.
1 In the tree, select the requested object.
Barcode ID / Order ID
Primary Tube ID
Tests ordered
Secondary Tube ID
(selected Tube)
Tube barcode
What workgroup the tube is
assigned to
Displays requested aliquot
volume (only if Secondary
Tube is highlighted).
Displays error message (if ap
-
plicable).
Opens the Sample Informa-
tion dialog box. Refer to Dis-
playing Sample Information
on page 17.
)( Quick Guide Retrieve Information
20
Menu and Tool Bar Functions
Menu )LOH:
Functions used for printing and closing the dialog box.
Menu 2SHUDWLRQV and corresponding tool bar buttons:
Refresh Routing Information: Refreshes data.
Find Opens the “Find” dialog box.
Select the search criteria and click )LQG1H[W.
Enter Order No.
Enter Primary Tube Barcode
Enter Secondary Tube Barcode
Searches above actual position
Searches below actual position
Apply Filter Opens the “Define Display Filter” dialog
box.
Select the filter criteria and click 6HW
)LOWHU.
Shows Routing Information of previous
runs.
Only orders/
tubes that
have a check
mark are
listed.
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The tecan GENESIS FE500 is an easy-to-use, yet fully automated workstation designed for clinical diagnostic laboratories. With its ability to perform a wide range of molecular biology applications, including nucleic acid extraction, PCR setup, and real-time PCR, the FE500 helps streamline laboratory workflows and improve efficiency. Featuring a user-friendly interface, the FE500 offers customizable protocols, barcode reading capabilities, and flexible sample handling options, making it ideal for laboratories of all sizes.
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