GALENVS MagnetiQ Operating instructions

Brand: GALENVS

Model: MagnetiQ

Type: Operating instructions

GALENVS MagnetiQ empowers rapid DNA extraction from blood and cell samples, enabling various downstream applications like PCR, qPCR, DNA methylation studies, and copy number variation studies. It streamlines the process with magnetic bead-based combined lysis and capture, eliminating the need for centrifugation and organic solvents. Amenable to automation, it offers faster magnetic collection, reduced clogging, and room-temperature operation.

BC0100-10 / BC0250-10 / BC/0096-10BC0016-10 v1.0

Blood & Cell DNA Extraction kit

Introduction

The Galenvs magnetiQ Blood & Cell DNA Extraction Kit enables a rapid and efficient method of genomic

DNA (gDNA) isolation and purification from whole blood samples and mammalian cell suspensions. Our

magnetic bead technology allows for a combined lysis and capture step and efficient washing steps leading

to high yields of concentrated gDNA free of cell debris, salts, lipid, or protein contamination under 20

minutes.

Applications

• Next Generation Sequencing (NGS)

• PCR and qPCR gene amplification

• DNA methylation studies

• Copy number variation (CNV) studies

Features

• Amenable to high-throughput methods and automation (Kingfisher Flex, Allsheng

AutoPure96, etc.)

• Faster and simpler magnetic collection and resuspension steps

• Reduced clogging and contaminant/cell debris carryover

• Eliminates organic solvent hazardous waste

• Requires only the use of a magnetic rack and standard pipettes

• Does not require centrifugation steps

• Storage and operation at room temperature

Performance

Yield and Purity

In comparing the Galenvs magnetiQ Blood & Cell kit to industry leading products (DNeasy Blood & Tissue

by Qiagen), 100 L of whole human blood (Potassium/EDTA stabilized) are processed as described by

manufacturer recommended protocols (Figure 1).

BC0100-10 / BC0250-10 / BC/0096-10BC0016-10 v1.0

Figure 1: Extraction yield of gDNA from 100 L of whole blood using Galenvs magnetiQ Blood/Cell kit and

Qiagen DNeasy Blood & Tissue kit. Quantification of gDNA performed using Qubit BR DNA fluorescent assay

measurement (n=3)

Table 1 : A260/A280 purity ratios of eluted gDNA samples from 100 L whole blood measured by UV/Vis nanodrop

(n=3)

A260/A280

Galenvs

1.83  0.03

Qiagen

2.04  0.12

Galenvs Qiagen

µg/µL

gDNA Yield

BC0100-10 / BC0250-10 / BC/0096-10BC0016-10 v1.0

-100

400

900

1400

1900

2400

15 20 25 30 35 40

RFU

Cycle

pre-GAPDH Quantification

Galenvs

Qiagen

qPCR

Both quantitative PCR (qPCR) (Figure 2) and gel electrophoresis results (Figure 3) confirm the higher recovery yields

obtained with Qubit BR DNA assay. Eluted gDNA is added to the qPCR master mix (NEB Luna) containing primers

for pre-GAPDH housekeeping gene.

Figure 2: Amplification of the pre-GAPDH gene by qPCR from 100 L of whole blood comparing Galenvs and

Qiagen extraction kits

Figure 3: Gel electrophoresis of extracted gDNA from 100 L whole blood samples (4x) comparing Galenvs and

Qiagen kits. Galenvs isolated gDNA is higher molecular weight while Qiagen extraction shows DNA smears below

gDNA bands

Galenvs

23.52

Qiagen

24.48

BC0100-10 / BC0250-10 / BC/0096-10BC0016-10 v1.0

Sample Volume

To illustrate the scalability of the Galenvs protocol, higher volumes of whole blood were processed while scaling the

lysis buffer volume accordingly. The elution volumes can be further tuned to obtain the desired final concentration

of isolated gDNA. Figures 4 and 5 show the yield and purity, respectively, from varying volumes of whole blood. The

total yield of gDNA increases linearly and matches the scaling factor of processed whole blood volume – while

maintaining high purity ratios. This is also confirmed in the gel electrophoresis analysis (Figure 6).

Figure 4: Total extraction yield of gDNA from varying

whole blood volumes

Figure 5: Extracted gDNA purity from whole blood

samples of different volumes

Figure 6: Gel electrophoresis of extracted gDNA from 100 L, 300 L, and 1000 L of whole blood samples

processed with Galenvs magnetiQ kit

Specifications

2.50

7.41

24.34

100 µL 300 µL 1000 µL

µg

Blood Sample Volume (µL)

Total gDNA Yield

1.85 1.89 1.91

0.5

1.5

2.5

100 µL 300 µL 1000 µL

Blood Sample Volume (µL)

A260/A280

BC0100-10 / BC0250-10 / BC/0096-10BC0016-10 v1.0

Sample Type

Blood, Cells, Bacteria

Quantity

100 or 250 assays / Prefilled plates (16-96 preps)

Elution Volume

100 µl

Processing Mode

Automated – Manual

Sample Volume

100 µL – 1000 µL

Binding Technology

Magnetic beads

Binding Capacity

Scalable

Components

(i) Lysis/Binding Buffer

(ii) Wash Buffer #1

(iii) Wash Buffer #2

(iv) Elution Buffer

(v) Proteinase K

Storage

Room temperature

Product codes

100 preps

BC0100

250 Preps

BC0250

Prefilled plates 96-well

BC0096

Prefilled plates 16-well

BC0016

galenvs.com

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GALENVS MagnetiQ Operating instructions

GALENVS MagnetiQ Operating instructions

GALENVS MagnetiQ Operating instructions

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