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Thermo Fisher Scientific iEMS Incubator Shaker User manual

  • Hello! I'm a chat assistant and I have reviewed the user manual for the Thermo Scientific iEMS Incubator/Shaker, including models iEMS 5112200 and iEMS 5112207. This manual provides information on installation, operation, and maintenance of the device, which is capable of incubating and shaking microplates with precise temperature control and variable shaking speeds. I'm ready to answer any questions you may have about its features and functionality.
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Thermo Scientific
iEMS Incubator/Shaker
User Manual
Rev. 3.4
Thermo Scientific
iEMS
Incubator / Shaker
User Manual
Rev. 3.4, Cat. no. 1506200A
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 2
Thermo Scientific iEMS Incubator / Shaker, Cat. no. 5112200 and 5112207
User Manual Rev. 3.4; June 2009, Cat. no. 1506200A
Copyright
Copyright © 2009 Thermo Fisher Scientific Corporation. All rights reserved.
Printed in Finland. Reproduction of the accompanying user documentation in
whole or in part is prohibited.
Trademarks (® and ™)
iEMS and Microtiter are trademarks of Thermo Fisher Scientific.
All other trademarks and registered trademarks are the property of their
respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services
at any time to incorporate technological developments. This manual is subject
to change without prior notice as part of a continuous product development.
Although this manual has been prepared with every precaution to ensure
accuracy, Thermo Fisher Scientific assumes no liability for any errors or
omissions, nor for any damages resulting from the application or use of this
information. This manual supersedes all previous editions.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising
out of the use or inability to use this product.
Contact information
Thermo Fisher Scientific Oy
P.O. Box 100, FI-01621 Vantaa, Finland
Tel. +358-9-329 100, Fax +358-9-3291 0415
www.thermo.com
Table of Contents
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 3
Table of Contents
1 Safety Symbols and Markings....................................................................................... 7
2 About the User Manual .................................................................................................. 8
3 Introduction to the iEMS™ Incubator / Shaker ........................................................... 9
4 Functional Description ................................................................................................. 10
5 Installation ....................................................................................................................14
6 Operation ...................................................................................................................... 18
7 Maintenance.................................................................................................................. 34
8 Troubleshooting Guide ................................................................................................ 39
9 Technical Specifications............................................................................................... 44
10 Ordering Information ................................................................................................... 48
11 Warranty Certificate ..................................................................................................... 49
12 Index .............................................................................................................................. 50
13 Glossary and Abbreviations ........................................................................................ 53
Appendix A: Thermo Scientific iEMS Incubator / Shaker Quick Reference Guide ....................... 54
Appendix B: Certificate of Decontamination ................................................................................... 55
Appendix C: Thermo Scientific iEMS Incubator / Shaker Feedback Form.................................... 56
Appendix D: Addresses...................................................................................................................... 57
Notes ............................................................................................................................. 58
Table of Contents
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 4
Table of Contents
1 Safety Symbols and Markings....................................................................................... 7
2 About the User Manual .................................................................................................. 8
3 Introduction to the iEMS™ Incubator / Shaker ........................................................... 9
3.1 Intended use ....................................................................................................................9
3.2 Principle of operation...................................................................................................... 9
3.3 Advantages of using iEMS Incubator / Shaker .............................................................. 9
4 Functional Description ................................................................................................. 10
4.1 iEMS Incubator / Shaker front view.............................................................................. 10
4.2 iEMS Incubator / Shaker rear view............................................................................... 11
4.3 Keyboard and display ................................................................................................... 12
4.4 Thermal microplate holder ........................................................................................... 13
5 Installation ....................................................................................................................14
5.1 Installation check list ..................................................................................................... 14
5.2 What to do upon delivery ............................................................................................. 14
5.2.1 How to unpack ............................................................................................................ 14
5.2.2 Checking delivery for completeness ......................................................................... 15
5.2.3 Checking for damage during transport ..................................................................... 15
5.2.4 Climatic requirements ................................................................................................ 15
5.2.5 Things to avoid ........................................................................................................... 15
5.2.6 Technical prerequisites .............................................................................................. 16
5.3 Setups before you put the instrument into operation................................................. 16
5.3.1 Voltage selector .......................................................................................................... 16
5.3.2 Mains supply cable..................................................................................................... 17
5.3.3 Setup ...........................................................................................................................17
6 Operation ...................................................................................................................... 18
6.1 Principle of operation.................................................................................................... 18
6.2 Programming protocols................................................................................................ 19
6.3 Slot status – control LEDs and audio signal................................................................. 22
6.4 Normal operation .......................................................................................................... 23
6.4.1 Placing the microplate................................................................................................ 23
6.4.2 Before pressing start .................................................................................................. 25
6.4.3 Operation .................................................................................................................... 26
6.4.3.1 Starting the operation................................................................................................. 26
6.4.3.2 Checking the operation time ...................................................................................... 26
6.4.3.3 Starting a program during operation in another slot................................................ 26
6.4.3.4 Checking the operation stop time.............................................................................. 27
6.4.3.5 Stopping the operation .............................................................................................. 27
6.4.3.6 Turning off the audio signal ....................................................................................... 27
6.4.4 Removing the microplate ........................................................................................... 28
6.5 Preheating and heating ................................................................................................. 30
6.6 Shaking .......................................................................................................................... 32
6.7 Verifying incubation and shaking parameters ............................................................. 33
Table of Contents
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 5
7 Maintenance.................................................................................................................. 34
7.1 Regular and preventive maintenance .......................................................................... 34
7.1.1 General ........................................................................................................................ 34
7.1.2 Immediate ................................................................................................................... 34
7.2 How to replace fuses..................................................................................................... 35
7.3 Disposal of materials..................................................................................................... 36
7.4 Decontamination procedure......................................................................................... 36
7.4.1 How to decontaminate the instrument surface......................................................... 37
7.4.2 How to decontaminate the thermal microplate holder............................................. 37
7.5 How to pack for service ................................................................................................ 38
7.6 Disposal of the instrument............................................................................................ 38
8 Troubleshooting Guide ................................................................................................ 39
8.1 Limitations .....................................................................................................................39
8.2 Warnings and cautions ................................................................................................. 39
8.2.1 Installation ................................................................................................................... 39
8.2.2 General ........................................................................................................................ 40
8.2.3 Temperature ............................................................................................................... 41
8.2.4 Shaking........................................................................................................................ 41
8.2.5 Operation .................................................................................................................... 42
8.2.6 Maintenance................................................................................................................ 42
8.3 Service request protocol............................................................................................... 43
9 Technical Specifications............................................................................................... 44
9.1 General specifications................................................................................................... 44
9.2 Incubator specifications ................................................................................................ 45
9.3 Shaker specifications .................................................................................................... 45
9.4 Safety specifications ..................................................................................................... 46
9.5 In conformity with the requirements............................................................................ 47
10 Ordering Information ................................................................................................... 48
10.1 List of accessories and recommended spare parts..................................................... 48
11 Warranty Certificate ..................................................................................................... 49
11.1 Warranty limitations ...................................................................................................... 49
12 Index .............................................................................................................................. 50
13 Glossary and Abbreviations ........................................................................................ 53
13.1 Keywords for web pages .............................................................................................. 53
Appendix A: Thermo Scientific iEMS Incubator / Shaker Quick Reference Guide ....................... 54
Appendix B: Certificate of Decontamination ................................................................................... 55
Appendix C: Thermo Scientific iEMS Incubator / Shaker Feedback Form.................................... 56
Appendix D: Addresses...................................................................................................................... 57
Notes ............................................................................................................................. 58
List of Figures and List of Tables
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 6
List of Figures
Fig. 4.1 iEMS Incubator/Shaker front view ....................................................................... 10
Fig. 4.2 iEMS Incubator/Shaker rear view ........................................................................ 11
Fig. 4.3 iEMS Incubator/Shaker keyboard ........................................................................ 12
Fig. 4.4 iEMS Incubator/Shaker display ............................................................................ 12
Fig. 4.5 Thermal microplate holder .................................................................................. 13
Fig. 5.1 Opening the voltage selector/fuse block assembly ............................................. 16
Fig. 5.2 Selecting the correct voltage............................................................................... 17
Fig. 6.1 Principle of operation .......................................................................................... 18
Fig. 6.2 Microplate in the thermal microplate holder....................................................... 23
Fig. 6.3 Inserting the thermal microplate holder into a slot.............................................. 24
Fig. 6.4 Pushing down the microplate holder to latch it properly .................................... 24
Fig. 6.5 Removing the microplate holder – lifting the handle .......................................... 29
Fig. 6.6 Removing the microplate holder – pulling the holder out................................... 29
Fig. 7.1 Opening the voltage selector/fuse block assembly ............................................. 35
Fig. 7.2 Fuse block assembly ........................................................................................... 35
List of Tables
Table 5.1 Installation check list ........................................................................................... 14
Table 6.1 Speed settings of the shaker ............................................................................... 21
Table 6.2 LED color codes.................................................................................................. 22
Chapter 1: Safety Symbols and Markings
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 7
1 Safety Symbols and Markings
These symbols are intended to draw your attention to particularly important
information and alert you to the presence of hazards as indicated.
Safety symbols and markings used on the iEMS Incubator / Shaker
Power ON
Power OFF
SN Serial number
REF Catalog number
Date of manufacture
Consult Instructions for Use
WEEE symbol This product is required to comply with the European Union’s Waste
Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC.
Warning markings used in the documentation
Warning: Risk of electric shock.
Warning: Biohazard risk.
Warning: Hot surface, risk of burns.
Warning: Risk of injury to the user(s).
Caution: Risk of damage to the instrument, other equipment or loss of
performance or function in a specific application.
Other markings used in the documentation
Note: Marks a tip, important information that is useful in the optimum
operation of the system, or an item of interest.
Chapter 2: About the User Manual
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 8
2 About the User Manual
The user manual has been written for the actual end user (for example,
laboratory technician) and provides information on the Thermo Scientific iEMS
Incubator/Shaker, including installation and operating instructions.
Read the manual in its entirety prior to operating the instrument.
The user manual has been designed to give you the information you need to:
• Review safety precautions
• Install the iEMS Incubator/Shaker
• Carry out incubating and shaking procedures
• Perform basic maintenance procedures
• Troubleshoot the instrument performance
• Maintain the instrument
The user manual also describes features and specifications of the iEMS
Incubator/Shaker hardware and onboard software.
Chapter 6 Operation explains the operating procedures.
The user should be familiar with the contents of Chapter 7 on maintenance.
For warranty and ordering information, refer to Chapters 10 Ordering
Information and 11 Warranty Certificate.
In an effort to produce useful and appropriate documentation, we appreciate
your comments on this document to your local Thermo Fisher Scientific
representative.
Chapter 3: Introduction to the iEMS Incubator / Shaker
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 9
3 Introduction to the iEMS™ Incubator / Shaker
The iEMS Incubator/Shaker consists of three modules, each module having a
capacity of three (3) microplates at a time.
3.1 Intended use
The iEMS Incubator/Shaker is a high-quality standalone microplate incubator
and orbital shaker intended for laboratory research use by professional
personnel. The instrument is used to incubate and shake microplates that meet
the SBS standards with a temperature of up to 40°C and a shaking speed of up
to 1400 rpm.
For verification of the entire system, it is recommended that Good Laboratory
Practices (GLP) be followed to guarantee reliable analyses.
Use for self-testing is excluded.
3.2 Principle of operation
The iEMS Incubator/Shaker consists of a keyboard and three (3) incubation
modules. Each module has three incubation slots for microplates. All slots can
be used for processing microplates individually within certain limitations. A
control LED above each slot indicates the status of that slot.
The keyboard is used to enter incubating and shaking parameters and to start
thus programmed protocols. These protocols can be the same for all slots or
entered individually for each slot. The parameters are stored in the instrument
memory until new parameters are entered. It is possible to change the
parameters of one slot without affecting the parameters of the other slots.
In one module, all slots have the same incubation temperature and they shake
at the same speed. The slots should be preheated to the programmed target
temperature before protocols are started.
3.3 Advantages of using iEMS Incubator / Shaker
The iEMS Incubator/Shaker provides several advantages relating mainly to the
principle of operation in that it has:
• Exceptional temperature uniformity across the plate
• Efficient orbital shaking
• Capacity of up to nine (9) microplates
Chapter 4: Functional Description
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 10
4 Functional Description
The iEMS Incubator/Shaker uses an individual thermal microplate holder
designed for ease of use and uniform heating of each microplate. Temperature
variation is less than 0.3°C across the microplate. To eliminate temperature
gradients and edge effects, microplates are evenly heated from all sides. This
ensures assay reproducibility.
Both the lid and the bottom of the thermal microplate holders are heated
electrically via connectors in the plate carrier. An upper heater within the top of
the module prevents condensation from forming within the microplate holder.
For effective mixing, a powerful variable-speed orbital shaker is incorporated.
Featuring an orbit of 1 mm and speeds from 400 to 1400 rpm in 250 rpm
increments, the shaker motion ensures efficient mixing of even very viscous
liquids. All slot positions within a module are shaken simultaneously.
Each slot of the iEMS Incubator/Shaker has a control LED (light-emitting diode)
that displays the status of the slot. The LED will change to a specific color based
on the status of the slot. When the incubation and shaking operations end, the
instrument also gives an audio signal.
4.1 iEMS Incubator / Shaker front view
1. Display
2. Keyboard
3. Control LED (light-emitting diode)
4. Incubation slot
5. Incubation module with three (3) slots
6. Mains input socket
7. Mains switch
8. Voltage selector and fuses
Fig. 4.1 iEMS Incubator/Shaker front view
6
7
8
5
4
3
2
1
Chapter 4: Functional Description
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 11
4.2 iEMS Incubator / Shaker rear view
1. Cooling-air inlet
2. DIP switches (not in use)
3. Device link RS-485 (not in use)
4. Computer RS-232C/device link RS-485 (not in use)
5. Cooling-air outlet
6. Type label
Fig. 4.2 iEMS Incubator/Shaker rear view
2 1 3 4 5 6
Chapter 4: Functional Description
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 12
4.3 Keyboard and display
Fig. 4.3 iEMS Incubator/Shaker keyboard
Listed below are the functions of the keyboard keys:
stop Stops the ongoing operation.
temp Sets the incubation temperature ON or OFF. Used for starting
preincubation (preheating) of the thermal microplate holders to
the temperature set in processing protocol parameters.
shake Starts shaking with the speed and time set in processing
protocol parameters.
start Starts operation defined by processing protocol parameters.
0 – 9 Are used for entering slot numbers and parameter values.
C Clears an incorrectly keyed numeric value.
↵ Enters a keyed value or continues the program.
Fig. 4.4 iEMS Incubator/Shaker display
The alphanumeric liquid crystal display (LCD) has two (2) lines with up to
20 characters per line.
TEMPERATURE 37.0ºC
INC TIME 00:30:00
Chapter 4: Functional Description
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 13
4.4 Thermal microplate holder
1. Detachable cover with heating element
2. Thermal and sensor contacts
3. Bottom with heating element
4. Adjustment screws
5. Handle
A1 Position of the A1 well on the microplate
Fig. 4.5 Thermal microplate holder
The thermal microplate holder is designed for holding the microplate during the
incubation process, ensuring accurate temperature control.
Note: Adjust the screws (Fig. 4.5, item 4) with a screwdriver so that the
microplate is held firmly in place during shaking.
1
2
3 4
A1
5
Chapter 5: Installation
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 14
5 Installation
5.1 Installation check list
The chapter on installation will contain an outline of the points mentioned in the
check list below.
Table 5.1 Installation check list
Tick Item
Unpack the iEMS Incubator/Shaker instrument carefully. Refer to 5.2.1.
Keep the original packaging and packing material for future transportation.
Check the delivery for completeness. Refer to 5.2.2.
Check for damage during transport. Refer to 5.2.3.
Place the instrument on a normal laboratory bench, taking into account
both the climatic and technical prerequisites. Refer to 5.2.4 and 5.2.6. Leave
sufficient clearance on both sides and at the rear of the unit.
Install the instrument. Refer to 5.3.
Connect the mains supply cable to the mains input socket. Refer to 5.3.2.
5.2 What to do upon delivery
5.2.1 How to unpack
Move the unpacked instrument to its site of operation. Unpack the iEMS
Incubator/Shaker instrument and accessories carefully with the arrows on the
transport package pointing upwards. The following notes and instructions are
sent with the instrument and are immediately available when you open the
package:
• Warranty Certificate card
• packing instructions/packing list
• transportation damage and discrepancy report
• Manufacturing / Quality Report
• iEMS Incubator/Shaker User Manual
Caution: Do not touch or loosen any screws or parts other than those specially
designated in the instructions. Doing so might cause misalignment and
will invalidate the instrument warranty.
To lift the instrument, put your fingers under the bottom on both sides and lift it
with your back straight.
Caution: When unpacking the instrument, it is recommended that two people lift
the instrument together, taking proper precautions to avoid injury.
Retain the original packaging and packing material for future transportation. The
packaging is designed to assure safe transport and minimize transit damage.
Use of alternative packaging materials may invalidate the warranty. Also retain
all instrument-related documentation provided by the manufacturer for future
use.
Chapter 5: Installation
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 15
5.2.2 Checking delivery for completeness
Check the enclosed packing list against order. In case of any deviations, contact
your local Thermo Fisher Scientific representative.
5.2.3 Checking for damage during transport
Visually inspect the transport package, the instrument and the accessories for
any possible transport damage.
If the carton has been damaged in transit, it is particularly important that you
retain it for inspection by the carrier in case there has also been damage to the
instrument.
Neither the manufacturer nor its agents can be held responsible for any damage
incurred in transit, but the manufacturer will make every effort to help obtain
restitution from the carrier. Upon receipt of the carrier's inspection report,
arrangements will be made for repair or replacement.
Visually check all interconnections in the basic instrument. Check that there are
no loose parts inside the instrument.
If any parts are damaged, contact your local Thermo Fisher Scientific
representative.
5.2.4 Climatic requirements
When you set up your iEMS Incubator/Shaker, avoid sites of operation with
excess dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive
moisture or large temperature fluctuations.
• Make sure the working area is flat, dry, clean and vibration-proof and leave
additional room for accessories, cables, reagent bottles, etc.
• Leave sufficient space (at least 10 cm) on both sides and at the back of the
unit to allow adequate air circulation.
• Make sure the ambient air is clean and free of corrosive vapors, smoke and
dust.
• Make sure the ambient temperature range is between +10°C (50°F) and
+40°C (104°F).
• Make sure relative humidity is between 10% and 90% (non-condensing).
The iEMS Incubator/Shaker does not produce operating noise at a level that
would be harmful. No sound level measurements are required after installation.
Warning: Do not operate the instrument in an environment where potentially
damaging liquids or gases are present.
5.2.5 Things to avoid
Do not smoke, eat or drink while using the iEMS Incubator/Shaker. Wash your
hands thoroughly after handling test fluids. Observe normal laboratory
procedures for handling potentially dangerous samples. Use proper protective
clothing. Use disposable gloves. Ensure that the working area is well ventilated.
Never spill fluids in or on the equipment.
Chapter 5: Installation
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 16
5.2.6 Technical prerequisites
Place the instrument on a normal laboratory bench. The net weight of the
instrument is 30 kg [66 lbs.].
The instrument operates at voltages of 100 – 120 Vac, 220 – 240 Vac and the
frequency range 50/60 Hz.
5.3 Setups before you put the instrument into operation
5.3.1 Voltage selector
The voltage selector/fuse block assembly (Fig. 5.1) is located on the left-hand
side (Fig. 4.1, item 8) of the instrument. Check that the selected voltage
corresponds to the local voltage. You can see the current voltage setting
through the slit in the fuse holder (Fig. 5.2, item 3).
To change the selected voltage:
1. Remove the power cable from the power inlet.
2. Use a small flat screwdriver to pry the voltage selector/fuse block assembly
away from the power entry port housing and remove (Fig. 5.1).
Fig. 5.1 Opening the voltage selector/fuse block assembly
3. Remove the voltage selector module (Fig. 5.2, item 4), rotate it 180° and
replace. Make sure that the correct voltage value is visible through the slit at
the end of the voltage selector/fuse block assembly (Fig. 5.2, item 3).
Chapter 5: Installation
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 17
1. Fuse
2. Fuse holder
3. Slit showing the correct voltage
4. Voltage selector module
Fig. 5.2 Selecting the correct voltage
4. Slide the fuse block assembly back into the power entry port housing. Snap
the assembly securely into place.
5. Reinsert the power cable.
5.3.2 Mains supply cable
Warning: Never operate your instrument from a power outlet that has no ground
connection. Never use a mains supply cable other than the Thermo
Scientific mains supply cable designed for your region.
1. Ensure that the mains switch (Fig. 4.1, item 7) at the back of the left side
panel is in the OFF position.
2. Connect the mains supply cable to the mains input socket (Fig. 4.1, item 6)
at the back of the left side panel.
3. Connect the instrument to a correctly installed line power outlet that has a
protective conductor that is grounded.
5.3.3 Setup
Power on the instrument. The firmware version is shown on the display.
Set the clock time
1. While in the READY state, press C to select the setup menu.
2. Select 1 for clock. Press ↵ (Enter).
3. Program the new time (hh mm ss) and press ↵ (24-hour clock).
The baud rate is used for service purposes only.
If you want to interrupt the setup, press stop to return to the READY state.
2
3
4
1
Chapter 6: Operation
Thermo Scientific iEMS Incubator / Shaker User Manual Rev. 3.4, Cat. no. 1506200A 18
6 Operation
6.1 Principle of operation
When you power on the instrument, it will be in the READY state. From the
READY state you can choose to modify the setup parameters (Section 5.3.3
Setup) or the protocol parameters (Section 6.2 Programming protocols). If you
have already programmed protocols, while in the READY state, you can start to
preheat the required slots (Section 6.5 Preheating and heating) before starting
the operation (Section 6.4 Normal operation). It is also possible to start shaking
the slots without heating as a separate function (Section 6.6 Shaking). For an
overview of the operations, see Fig. 6.1 below.
Fig. 6.1 Principle of operation
Power ON
READY
shake
start
Shaking only
SHAKING
ON
READY
Change protocol
p
arameters
Slot no.
1 – 9 or 0
for all slots
↵ (Enter)
Set protocol
parameters,
see 6.2
READY
Start
protocol
Slot requires
preheating
start
READY
Protocol
starts
tem
p
start
HEAT ON
READY
Slot no.
1 – 9 or 0
for all slots
Slot no.
1 – 9 or 0
for all slots
Slot no.
1 – 9 or 0
for all slots
/