Perform the pre‑run checklist ............................................... 142
Stage 3: Precipitate the DNA .............................................. 143
Stage 4: Centrifuge and dry DNA pellets ........................................ 144
Equipment required ....................................................... 144
Input samples ............................................................ 144
Stage 4 summary ......................................................... 144
Perform the pre‑run checklist ............................................... 144
Stage 4: Centrifuge and dry pellets ......................................... 145
Stage 5: Resuspend the pelleted DNA and prepare for hybridization ................. 146
Equipment and labware required ........................................... 146
Input samples ............................................................ 147
Reagent handling ......................................................... 147
Guidelines for pellet preparation ............................................ 148
Prepare the Axiom™ Propel Resuspension Buer ............................. 148
Prepare Hybridization Master Mix ........................................... 149
Stage 5 summary ......................................................... 149
Perform the pre‑run checklist ............................................... 150
Stage 5: Resuspend the pelleted DNA and prepare for hybridization ............ 151
Transfer the hybridization-ready target to the half-skirted 96‑well PCR plate ...... 153
Stage 5A: In-process QC ...................................................... 157
Equipment and labware required ........................................... 157
Input samples ............................................................ 157
Reagents required ........................................................ 157
Stage 5A summary ....................................................... 158
Perform the pre‑run checklist ............................................... 158
Stage 5A: In-process QC ................................................. 159
Transfer hybridization-ready target in Hyb-Ready Plate to QC plate ............. 161
Stage 6: Denature the target and transfer to hybridization tray ...................... 167
Equipment and labware required ........................................... 167
Input samples ............................................................ 167
Stage 6 summary ......................................................... 167
Perform the pre‑run checklist ............................................... 168
Warm the array plate to room temperature ................................... 168
Prepare hybridization-ready samples stored at –20°C ......................... 168
Stage 6: Denature the target and transfer to hybridization tray ................. 169
Transfer hybridization-ready target in Hyb-Ready Plate to hybridization tray ...... 169
Perform array plate clamping and o-line hybridization ........................ 172
Stage 7: Preparing ligation, stain, stabilization reagent trays, and scan trays for
the GeneTitan™ MC Instrument ............................................... 175
Equipment and labware required ........................................... 175
Reagent handling ......................................................... 175
Reagent preparation for Stage 7: Prepare GeneTitan™ reagents ................ 178
Prepare Ligate Master Mix ................................................. 179
Prepare Stain 1 Master Mix ................................................ 180
Prepare Stain 2 Master Mix ................................................ 181
Prepare Stabilization Master Mix ............................................ 182
Contents
Axiom™ Propel Fast Wash Workflow, 96‑Array Format User Guide 7