Thermo Fisher Scientific ABI PRISM® 3700 User guide

Brand: Thermo Fisher Scientific

Model: ABI PRISM® 3700

Type: User guide

ABI PRISM® 3700 Instrument-Hardware-Missing Lanes Troubleshooting Module

This module is intended to troubleshoot runs that exhibit weak data or no data in the array view.

Please contact technical support if you have any questions.

Text in blue indicates a link to another portion of the document.

Observations

Possible Causes

Recommended Actions

Loss in communication

Restart the system

Optics

Check the CCD calibration window to verify the presence of raman lines. Refer to

the Checking CCD window module. If raman lines are not present, open a service

call.

Check the water reservoir. Replenish the water and verify that the fluid line

sinkers for the tubing are at the bottom of the reservoir.

Verify that the autoloader tips are screwed in. They should be finger tight.

Verify the fittings above the sample transfer syringes are finger tight.

Locate the source of the bubbles, refer to the Checking for bubbles module.

Sample loading issue

bubbles in the sample transfer

lines or syringe

Replace the sample transfer syringe(s) using the change syringe wizard.

Clogged loading tips

Replace the autoloader tips. Use the Change Tips wizard.

Entire array is missing

data

Electrophoresis issues

Refer to Electrophoresis Troubleshooting Module

Check the capillary positions. Verify that the loading end of the array is sitting in

the wells of the load bar.

Even Lane Failure

Capillaries are not in the loading

wells.

Check for broken capillaries. If breakage is seen, replace the array.

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Observations

Possible Causes

Recommended Actions

Improper drainage of the load bar

Verify that the fluid line sinkers are at the bottom of the buffer reservoir and that

the tubing in the buffer reservoir is not crimped. If crimping is observed,

straighten the tubing and setup another run.

Odd Lane Failure

Upper wells of the load bar are

not filling properly.

Improper seating of the load bar. Verify that the load bar is not loose.

Check the water reservoir. Replenish the water and verify that the fluid line

sinkers for the tubing are at the bottom of the reservoir.

Verify that the autoloader tips are screwed in until finger tight.

Verify that the fittings above the syringes are finger tight.

Refer to the Checking for Bubbles module to determine the source of the bubbles.

Replace the left or right sample transfer syringe using the change syringe wizard.

Blocks of eight

Alternating pattern of 8

good samples and 8

failures.

Sample loading issue

bubbles in the sample transfer

lines

clogged loading tips

Replace the autoloader tips.

Check the capillary positions. Verify that they are sitting in the wells of the load

bar.

Load bar assembly

Improper seating of the load bar. Ensure that the load bar fits snugly in the

instrument.

Block Failure

Clogged loading tips

Replace the autoloader tips.

Load bar assembly

Improper seating of the load bar. Ensure that the load bar fits snugly in the

instrument.

One side of array affected

Spatial calibration issue

Check the CCD calibration window, refer to the CCD Calibration raman line

intensity should be fairly uniform across the array. If not, open a service call

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Observations

Possible Causes

Recommended Actions

Run another spatial and optimize the cuvette temperature until the left to right

variation across the array is 1:3 or less. Refer to the Spatial Troubleshooting

Module.

Broken capillaries

Check the capillaries for breakage. Replace array or if less than 8 capillaries are

affected, block the capillaries in Data Collection.

Capillaries not positioned in load

bar correctly

Reposition the capillaries such that they are sitting within the wells of the load bar.

Autoloader tips may be clogged

or bent

Replace the autoloader tips.

Spatial calibration may have

shifted

Run a spatial calibration.

Centrifuge sample before loading on instrument.

Sporadic lane failure

Sample problems

bubbles in the sample wells

sample failure

Contact technical support to troubleshoot.

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Restarting the 3700 DNA Analyzer

IMPORTANT To prevent firmware and software memory problems, we recommend that you restart the instrument and the software once

a week.

To shut down and restart the instrument:

Step 1: The instrument should not be running or extracting data.

Step 2: Close the 3700 Data Collection software by selecting Shutdown from the File menu.

Note You cannot use the Close button to exit the software.

Step 3: Close the OrbixWeb Daemon software by right-clicking on its button in the taskbar and selecting Close from the pop-up menu. If

you get a run-time message, click OK to close the message.

IMPORTANT Do not shut down the OrbixWeb™ Daemon until after you have shut down the Data Collection program.

Step 4: Restart the computer. After logging in, OrbixWeb™ Daemon should have automatically launched. Do not start Data Collection at

this time.

Step 5:

a. Turn off the instrument using the On/Off button.

b. Wait 30 seconds.

c. Turn on the instrument.

Step 6: When the green status light is steady, wait 1 minute.

Step 7: Restart the 3700 Data Collection software.

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Fluid Line Sinkers Array Diagram

Fluid line sinkers

Loading end of

array

Broken capillaries along the length of the array can

be problematic if the array is not changed. Polymer

will still flow through the capillary causing buildup of

polymer in the area and affecting other capillaries.

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Load Bar

The load bar should fit snugly in the instrument. Grab the ends of the load bar and gently try to move the bar, if

movement is felt, push down on the load bar to engage it with the instrument. Try to move the load bar again, it

should feel snug in the instrument.

Load bar

Autoloader tips

Tips should be screwed to fingertight and the bottom of the

tips should be even.

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Loading End of Array

The loading-end header is the unit that holds the capillaries at the sample-loading end of the capillary array and aligns them with the

injection wells of the loading bar.

The diagram below shows how the loading-end header is held in the loading block.

Using the wizards

Step 1: Go to the Instrument pull down menu

Step 2: Select Wizards

Step 3: Select appropriate wizard

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Sample Transfer syringe

From syringe to

robot tip

From water

reservoir to

syringe

From syringe to

robot tip

From water

reservoir to

syringe

Contacting Technical Support

By phone: 1-800-831-6844, option 5

By email: ABTechnicalSupport@appliedbiosystems.com pn 4375410

Stock # 106GU14-01

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Thermo Fisher Scientific ABI PRISM® 3700 User guide

Brand: Thermo Fisher Scientific

Model: ABI PRISM® 3700

Type: User guide

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Thermo Fisher Scientific ABI PRISM® 3700 User guide

Thermo Fisher Scientific ABI PRISM® 3700 User guide

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