Gilson VERITY 1910 MS User manual

Brand: Gilson

Model: VERITY 1910 MS

Type: User manual

VERITY® 1910 MS Detector

User’s Guide

Trademarks

All product and company names are trademarks™ or registered® trademarks of their

respective holders. Use of the trademark(s) in this document does not imply any

aliation with or endorsements by the trademark holder(s).

VERITY® 1910 MS DETECTOR | USER’S GUIDE

TABLE OF CONTENTS

Safety | 5

Chemical Hazards | 7

Compressed Gas Hazards | 7

Dangerous High Voltages | 7

High Voltages and Solvents | 7

Protective Covers | 8

Pump Exhaust | 8

Spacing | 8

CHAPTER 1 | Introduction | 9

Description | 10

Inspect Shipment | 12

Customer Service | 12

Technical Speciﬁcations | 13

CHAPTER 2 | Installation | 15

Setup | 16

Stacking | 17

vac-chip™ Installation | 18

spraychip® Installation | 21

Rear Panel Connections | 24

Front Panel | 29

CHAPTER 3 | Auto Tune (Calibrate) | 35

Locate Required Items | 36

Pump Down | 36

Prepare the Syringe Pump | 37

Plumb the Syringe Pump | 38

Run the Auto Tune (Calibration) Procedure | 39

Flush the System | 39

CHAPTER 4 | Operation | 41

Prepare to Analyze | 42

Safety Interlock | 43

Power On | 44

Pump Down | 44

Power Down | 45

CHAPTER 5 | Maintenance | 47

Maintenance Schedule | 48

General Maintenance | 48

vac-chip™ Assembly | 50

vac-chip | 50

spraychip® | 55

Spraychip Flange | 60

Source, Filter, and Detector (SFD) Assembly | 61

Liquid Flow Rate Measurement | 62

Split Flow Interface | 64

TABLEOFCONTENTS | VERITY® 1910 MS DETECTOR

TABLE OF CONTENTS

CHAPTER 6 | Troubleshooting | 71

General Warnings | 72

Troubleshooting Table | 72

Repair and Return Policies | 74

APPENDIX A | Flow Splitting | 75

Overview | 76

Equations | 77

Split Ratios | 79

APPENDIX B | Auxiliary I/O | 81

Overview | 82

Auxiliary I/O Port Pin Assignments | 83

Triggering Sources | 84

Analog Inputs and Outputs | 86

APPENDIX C | Parts and Accessories | 87

System | 88

Components | 88

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Chapter 1 | SaFetY

Read this chapter before installing and operating the instrument.

Only trained technical personnel in a laboratory environment may use the instrument

for non-medical, liquid handling purposes. For safe and correct use of the instrument,

operating and service personnel must follow all instructions contained in this guide

when installing, cleaning, and maintaining the instrument. All safety precautions must be

observed during all phases of operation, service, and repair of the instrument.

Failure to comply with these precautions or with warnings described in the user’s guide

violates safety standards of design, manufacture, and intended use of the instrument.

Gilson assumes no liability for customers failing to comply with these requirements.

The instrument has been certified to safety standards required in Canada, Europe, and

the United States. Refer to the rear panel label on the instrument and the Declaration of

Conformity document for the current standards to which the instrument has been found

compliant.

IN thIS Chapter

● Electronic and Hazard Symbols | 6

● Safety Notices | 7

● Chemical Hazards | 7

● Compressed Gas Hazards | 7

● Dangerous High Voltages | 7

● High Voltages and Solvents | 7

● Safety Precautions | 8

● Protective Covers | 8

● Pump Exhaust | 8

● Spacing | 8

SAFETY | VERITY® 1910 MS DETECTOR

SAFETY

Electronic and Hazard Symbols

The following safety symbols and notices may appear on the instrument or in this document:

Symbol

Explanation

~ Alternating current

Caution

Caution, risk of electric shock

WARNING

Warning, hazardous voltage

WARNING

Compressed

Gas

Warning, compressed gas

Warning, corrosive chemical

VERITY® 1910 MS DETECTOR | USER’S GUIDE

SAFETY

Safety Notices

The following safety notices may appear in this document:

WARNING

A potentially hazardous situation which, if not avoided, may result in serious injury

CAUTION

A potentially hazardous situation which, if not avoided, may result in minor or moderate

injury

NOTICE

A potentially hazardous situation which, if not avoided, may result in equipment damage

Chemical Hazards

Any chemicals used for analysis should be handled according to good laboratory practice.

They should also be stored, used, and disposed of in accordance with the manufacturer’s

specifications, as well as local and national regulations. Potentially hazardous chemicals can

be used with the VERITY® 1910 MS Detector. Use care when handling chemicals and wear

appropriate personal protective equipment (PPE).

The responsible individual must ensure that personnel are not exposed to hazardous levels of

toxic substances as outlined in the Material Safety Data Sheets (MSDS), or any documentation

provided by local governing bodies such as The Health Protection Agency (UK) or The

Occupational Safety and Health Administration (US).

Compressed Gas Hazards

The VERITY 1910 MS Detector requires a source of dry nitrogen gas for correct operation.

Always follow local health and safety regulations if handling cylinders of compressed gas.

Environmental monitoring must be deployed to detect and alert the user to the occurrence of

a reduction of oxygen due to a nitrogen leak in the environment. In case of oxygen reduction,

all personnel need to leave the room and alert the proper health and safety representative.

Dangerous High Voltages

Several internal parts of the VERITY 1910 MS Detector can supply dangerous voltages. When

the VERITY 1910 MS Detector is connected to the mains supply, dangerous high voltages can

be present even if the power is switched off.

High Voltages and Solvents

This system operates using high voltages. This can introduce additional hazards when certain

solvents are used. Ensure all solvents and instruments are properly connected, vented, or set

up with manufacturer approved settings.

SAFETY | VERITY® 1910 MS DETECTOR

SAFETY

Safety Precautions

The following safety precautions concerning the operation of the VERITY 1910 MS Detector

and the site in which it is used must be observed at all times in order to prevent injury and

damage to the system and associated instruments.

WARNING

Do not use the instrument if there are signs of visible damage.

Do not operate if site conditions are not within speciﬁcations.

Do not override any safety interlock.

Do not attempt to operate the instrument with the covers removed. High voltages are present

when the instrument is in operation.

Do not attempt to adjust or replace components other than those that have been described in

this user’s guide.

WARNING

The instrument is not designed to be operated in a potentially explosive atmosphere. It is the

responsibility of the customer to verify the operating environment meets the requirements of

a “non-hazardous area” with regards to potentially explosive atmosphere.

At least two people are required to lift or move the VERITY 1910 MS Detector.

The VERITY 1910 MS Detector should only be used with accessories that meet the

manufacturer’s speciﬁcations. Failure to do so may result in damage to the instrument and

lead to the loss of liability and warranty entitlements.

To prevent injury and damage to equipment, ensure the blanking plug is removed from the

exhaust port before activating the pumps.

Protective Covers

Unless specifically instructed, do not remove any protective covers. The product is sealed

at the factory. Damage or removal of the warranty seals leads to the loss of liability and

warranty entitlements.

Pump Exhaust

The pump exhaust from the VERITY 1910 MS Detector must be connected to a suitable

extraction system. Appropriate extraction must also be provided to the microspray area when

analyzing hazardous chemicals.

Spacing

Allow sufficient spacing around the VERITY 1910 MS Detector for proper cooling and for the

connection of mains plug, gas inlets, syringe pump, HPLC etc.

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Chapter 1

CHAPTER 1 | INTRODUCTION

IN THIS CHAPTER

● Description | 10

● Inspect Shipment | 12

● Customer Service | 12

● Technical Specifications | 13

INTRODUCTION | VERITY® 1910 MS DETECTOR

Description

The VERITY® 1910 MS Detector is a compact system that employs micro-engineering

technology to identify the chemical contents of solutions, including: spraychip®, vac-chip™,

and ionchip® technologies.

A chemical sample, dissolved in suitable solvents, is introduced into the spraychip, typically

from a highpressureliquidchromotography(HPLC) system or syringe pump. Gas phase ions

are formed and are introduced into the vacuum chamber through the vac-chip.

The ions introduced through the vac-chip are then focused using a series of electrostatic ion

optics. The ionchip filters the ions according to their mass-to-charge (m/z) ratios and directs

them to the detection system.

Software controls the settings of the VERITY 1910 MS Detector and related instruments.

Source, Filter, and Detector Assembly

The source, filter, and detector assembly (SFD) consists of the spraychip

flange, vac-chip assembly, ionguide, ionchip and detectorassembly. Refer to

Source, Filter, and Detector (SFD) Assembly on page 61.

Figure 1

VERITY® 1910 MS Detector

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Description

Split Flow Interface

The split flow interface (SFI) incorporates a built-in splitter that enables you to connect a high

flow system, such as an highpressureliquidchromotography(HPLC) system or MiDas™ to

the VERITY 1910 MS Detector. Gilson recommends using a flow rate range of 10µL–2 mL/min.

The SFI is also used for auto tuning or calibrating the VERITY 1910 MS Detector. Refer to

Chapter 3 | Auto Tune (Calibrate).

The SFI is shown in Figure 2. It is designed to use 1/32" OD PEEK tubing for the input and

waste lines. Various split ratios can be achieved by connecting a suitable length of PEEK

tubing to the waste (high flow) port. Refer to the FLOW SPLITTING appendix.

spraychip® Ion Source

The spraychip is a micro-electrospray ion source. Compared to conventional electrospray ion

sources, microspray provides the following advantages: increased ionization efficiency, lower

flow rates, which permits analysis of very small sample volumes over long periods of time,

and lower voltages required to provide efficient electrospray.

Microspray is a soft ionization technique. A voltage, generally between 0.7 and 1.5 kV,

is applied to an analyte dissolved in a suitable solvent, generally a mixture of water and

an organic solvent. The solvent passes through an emitter or capillary tip at a flow rate

of 300–1000 nL/min. The voltages and flow rates used in microspray are lower than for

conventional electrospray ionization. This means that lower volumes of sample and solvent

are consumed by the VERITY 1910 MS Detector.

Under the correct conditions, a plume of charged aerosol droplets forms from a Taylor cone.

Gas-phase analyte ions are subsequently formed from these aerosol droplets.

Ions produced by this method can have single or multiple charges. Generally, high molecular

weight compounds, for example, proteins, produce a series of multiply-charged ions.

Figure 2

The Split Flow Interface (SFI)

Grub

Screw

Main Body

Metal Collar

PEEK

Screw

Spraychip

Tubing

Connections

INTRODUCTION | VERITY® 1910 MS DETECTOR

Description

Vac‑Chip and Ion Optics

The vac-chip interface samples the ions from the microspray source and transports the

sampled ions into the vacuum chambers of the VERITY 1910 MS Detector.

After passing through the vac-chip, ions pass through a series of ion optics: a tube lens,

exit lens, ion guide and inter-quadrupole lens. Ions exiting the vac-chip are focused into the

ionchip® using these ion optics.

ionchip

The ionchip is a micro-engineered quadrupole mass analyzer based on patented technology.

It functions like a conventional quadrupole mass analyzer, acting as a mass filter, separating

ions in order of their mass-to-charge (m/z) ratios. However, it is about one hundredth the

volume of a conventional quadrupole mass analyzer.

Vacuum System

The vacuum system consists of a vacuum chamber coupled to a split flow turbo-molecular

pump, which is backed by a diaphragm pump. The vac-chip interface is pumped by an

additional small diaphragm pump.

All of the vacuum pumps are integrated internally in the VERITY 1910 MS Detector so there

are no external, floor-standing pumps. This significantly reduces equipment noise, footprint,

and size. There are also no complications associated with rotary pumps, such as back

streaming of pump oil and the use of oil traps.

The vacuum system comprises three sections:

● The vac-chip interface

● The ion guide chamber

● The analytical chamber

The pressures in the VERITY 1910 MS Detector are monitored using Pirani gauges which

also act as high-voltage interlocks. These gauges turn off any high voltages if the pressure

readings in the chambers are above a set safety value.

Inspect Shipment

Upon receipt of the instrument, inspect the exterior of all shipping cartons. All cartons should

arrive unopened and undamaged. If examination reveals that damage has occurred during

shipment, notify the carrier, and Gilson immediately. Refer to Customer Service on page 12.

Customer Service

Gilson, Inc. and its worldwide network of representatives provide customers with the

following types of assistance: sales, technical support, applications, and instrument repair.

If you need assistance, please contact your local Gilson representative. Detailed contact

information can be found at www.gilson.com. To help us serve you quickly and efficiently,

refer to BeforeCallingUs on page 74.

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Technical Speciﬁcations

NOTE

This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference, in which

case the user will be required to correct the interference at his/her own expense.

VERITY® 1910 MS Detector

Speciﬁcation Description

Communication Ethernet

Contact Control

Analog

Digital

Eight analog contacts: analog in (x4), analog out (x4)

Four digital contacts: digital in (x2), digital out (x2)

Detector Electron multiplier detector

Dimensions

(L x W x H)

55 x 35 x 25 cm*

23.5 x 16 x 10 in.**

*+ 5 cm (L x W) for ventilation

**+ 0.2 in (L x W) for ventilation

Dynamic Range Three orders of magnitude

Environmental

Conditions

Indoor use

Altitude Up to 2000 m

Temperature Range

Operation 15°C–30°C

Storage -10°C–50°C

Relative Humidity

Operation ~40%–80%, non-condensing

Storage ~10%–90%, non-condensing

Pollution Degree 2

Normally only non-conductive pollution occurs. Occasionally,

however, a temporary conductivity caused by condensation

must be expected.

Flow Rate Range 0.3–2000 µL/min

Interface O-axis vac-chip™ microengineered atmospheric pressure interface

Ionization Modes Positive and negative ESI modes

Ion Source Spraychip® electrospray ionization source

VERITY 1910 MS DETECTOR TECHNICAL SPECIFICATIONS (CONTINUED ON PAGE 14)

INTRODUCTION | VERITY® 1910 MS DETECTOR

Technical Speciﬁcations

VERITY® 1910 MS Detector

Speciﬁcation Description

Mass Accuracy

± m/z 0.3 in full scan*

*In a temperature controlled environment, 20°C ±3°C

Mass Analyzer ionchip® quadrupole mass ﬁlter

Mass Range m/z 50–1400 with ionchip® 150

Mass Resolution

m/z 0.7 ± FWHM*

*In a temperature controlled environment, 20°C ±3°C

Nitrogen Gas

Pressure 2–6 bar (29–87 psi)

Purity ≥ 99.5%

Tubing

Site must use either stainless steel or polytetraﬂuoroethylene (PTFE)

tubing.

Fitting

Stainless Steel 6 mm push lock ﬁtting

¼"–6 mm adapter available

Power Requirements

Line Voltage 100–240V AC ±10%

Line Frequency 50/60 Hz

Power 300 W (peak)

Overvoltage Category II

For transient overvoltages typically present on the

powersupply.

For temporary overvoltages on the power supply.

Pump Exhaust

Tubing

8 mm OD push lock ﬁtting

Pumping System Integrated oil-free pumps

Safety and

Compliance

The instrument has been certiﬁed to safety standards speciﬁed for Canada, Europe,

and the United States. Refer to the instrument rear panel label and the Declaration of

Conformity document for the current standards to which the instrument has been found

compliant.

Sample Inlet Compatible with standard capillaries and unions e.g., 1/32" tubing

Scan Modes Full scan, SIM (4 m/z channels), simultaneous scan/SIM, and timed SIM

Scan Rate m/z 1,500/s (7,500 pts/s)

Sensitivity 10 pg of reserpine yields a peak in SIM mode with a S/N ratio of 10:1 (RMS)

Software Control

PC control via Ethernet and TRILUTION® LC Software

Control via Ethernet and Gilson Glider Prep (GGP) Software and Masscape® Software

Weight 32 kg (70.5 lbs.)

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Chapter 2

CHAPTER 2 | INSTALLATION

IN THIS CHAPTER

● Setup | 16

● Stacking | 17

● vac-chip™ Installation | 18

● spraychip® Installation | 21

● Rear Panel Connections | 24

● Front Panel | 29

INSTALLATION | VERITY® 1910 MS DETECTOR

Setup

The VERITY® 1910 MS Detector must be installed on a lab bench and can only be operated

with the front cover closed.

CAUTION

Allow sucient spacing around the VERITY 1910 MS Detector for proper cooling and for the

connection of mains plug, gas inlets, syringe pump, HPLC etc.

The VERITY 1910 MS Detector is too heavy to be lifted or moved by one person

safely. To avoid personal injury and for general safety, if moving or lifting the

VERITY 1910 MS Detector, always have another person assist you. Always follow local

health and safety regulations.

The maximum weight that can be supported by the VERITY 1910 MS Detector is 132 lbs.

(60kg).

253 mm

(10 in.)

449 mm

(17.7 in.)

109 mm

(4.3 in.)

354 mm

(14 in.)

Figure 3

VERITY® 1910 MS Detector Dimensions

VERITY® 1910 MS DETECTOR | USER’S GUIDE

Stacking

The VERITY® 1910 MS Detector must be installed on a lab bench. The MiDas™ can be stacked

on top of the VERITY 1910 MS Detector.

Connect the drain tube to the outlet on the gutter provided with the MiDas. Place the

front feet of the MiDas through the holes in the gutter. Stack the MiDas with gutter on the

VERITY 1910 MS Detector.

Figure 4

Vertical Stacking Configuration

Gutter

Drain

Tube

Drain

Tube

INSTALLATION | VERITY® 1910 MS DETECTOR

vac-chip™ Installation

vac‑chip™ Installation

The vac-chip assembly houses the vac-chip and the tube lens assembly (Figure 5). The vac-chip

assembly will be fitted and removed from the VERITY 1910 MS Detector as part of routine

customer maintenance procedures whenever the vac-chip needs to be cleaned or replaced.

Refer to Remove the spraychip on page 55.

To fit or remove the vac-chip assembly follow the subsequent steps. The vac-chip assembly is

designed to be fitted or removed with the door of the VERITY 1910 MS Detector open.

NOTICE

Always wear powder-free gloves when handling all components of the vac-chip assembly.

Fingerprint residue can cause problems with the operation of these devices.

The VERITY 1910 MS Detector is shipped with a plastic insert in place of the vac-chip.

Remove this insert and install the vac-chip before operation.

1. Open the door to 90 degrees.

2. Loosen the four thumbscrews securing the vac-chip assembly in an alternating criss-cross

pattern.

3. Remove the four thumbscrews.

4. Gently pull the vac-chip assembly away from the source, filter, and detector assembly (SFD),

using the dowels as guides.

5. Fit the SFD cover plate to the VERITY 1910 MS Detector.

6. Lay the assembly face down on a clean lint free surface.

● The tube lens assembly is attached to the vac-chip ﬂange with ﬁve vacuum screws. The

vac-chip is located under this assembly.

Figure 5

The vac‑chip™ and Tube Lens Assembly

vac-chip

Flange

vac-chip

Screws

Tube

Lens

Assembly

vac-chip

VERITY® 1910 MS DETECTOR | USER’S GUIDE

vac-chip™ Installation

7. Use a Phillips screwdriver to partially unscrew the five vacuum screws in the following

pattern: 1,4,2,5,3 (Figure 6). Repeat until the screws are loose.

8. Gently lift the tube lens assembly away from the vac-chip flange.

9. Use a pair of tweezers to remove the plastic insert pre-installed in the vac-chip assembly.

10. Place the new vac-chip in the vac-chip flange ensuring that the larger face of the chip is

facing downwards.

NOTICE

The user must ensure that the small O-rings are present on the vac-chip ﬂange (under the

vac-chip) and the underside of the tube lens assembly. Also ensure that both O-rings are

properly seated.

Figure 7

How to Handle the vac‑chip™

11. Lower the tube lens assembly onto the vac-chip flange, it will locate with two dowels.

Ensure the assembly is lying parallel to the vac-chip flange.

Figure 6

Tube Lens Assembly and Vacuum Screws

INSTALLATION | VERITY® 1910 MS DETECTOR

vac-chip™ Installation

12. Use a Phillips screwdriver to sequentially tighten the five vacuum screws in the following

pattern: 1,4,2,5,3 (Figure 6), and repeat until the screws are tight.

NOTICE

Do not over tighten the vacuum screws, and do not continue to tighten screws if any

resistance is felt, in this case remove the assembly and retry.

CAUTION

The vacuum screws have been specially made for the VERITY 1910 MS Detector. Do not

substitute these screws unless they have been provided by Gilson.

13. Locate the vac-chip flange on the SFD, using the dowels as shown in Figure 8. Gently push

the vac-chip flange into place, do not force the flange into position.

NOTE

There is a single orientation where the holes and threaded sections will align.

NOTICE

If excessive resistance is felt when ﬁtting the assembly to the SFD, stop immediately,

remove the assembly and retry step 13.

14. Fit the four thumbscrews into the vac-chip assembly.

15. Tighten the four thumbscrews in an alternating criss-cross pattern until the screws are

tight.

NOTICE

If excessive resistance is felt when ﬁtting the assembly to the SFD, stop immediately,

remove the assembly, and retry steps 13–15.

Dowels

vac-chip

Assembly

Figure 8

vac‑chip™ Assembly Installation

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Gilson VERITY 1910 MS User manual

Gilson VERITY 1910 MS User manual

Gilson VERITY 1910 MS User manual

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