Thermo Fisher Scientific 32-Gauge Stainless Steel Needle Installation guide

Brand: Thermo Fisher Scientific

Model: 32-Gauge Stainless Steel Needle

Type: Installation guide

Thermo Fisher Scientific supplies stainless steel needles in two

sizes, 32-gauge and 34-gauge. This guide describes how to install

the 32-gauge stainless steel needle, which supports flow rates

from5to1000μL.

Overview

A fused-silica capillary typically connects the solvent path between

the grounding union held by the grounding bar of the Ion Max™ or

Ion Max-S API source and the tip of the electrospray needle that

protrudes from the nozzle of the ESI probe.

Because acetonitrile adversely affects a fused-silica capillary,

Thermo Fisher Scientific recommends the use of a stainless steel

needle and red PEEK™ tubing for applications that use acetonitrile

in the mobile phase.

The stainless steel needle connects the solvent path inside the probe

(see Figure 1). The red PEEK tubing connects the probe to the

grounding union held by the grounding bar of the Ion Max or

Ion Max-S API source (see Figure 2).

Figure 1. Solvent path inside the ESI probe

Figure 2. Connection between the probe and the grounding union

Required Parts and Tools

To install the 32-gauge stainless steel needle, you must have the parts

and tools listed below. The MS Accessory Kit includes red PEEK

tubing. The Metal Needle Kit, OPTON-53003, contains the

additional required parts with the exception of the wrench and the

Teflon™ tape.

Step 1: Inserting the Needle into the Probe

YTo insert the 32-gauge stainless steel needle into the ESI probe

1. Wearing talc-free gloves, straighten one end of the thread

that protrudes from the bore of the stainless steel needle

(see Figure 3), and then pull the thread out of the needle from

the opposite end.

Figure 3. Stainless steel needle with internal thread

2. Remove the adapter from the sample inlet of the ESI probe

(see Figure 4).

Figure 4. Adapter nut shown removed from the probe

32-Gauge Stainless Steel Needle Installation Guide

Contents

•Overview

•Required Parts and Tools

•Step 1: Inserting the Needle into the Probe

•Step 2: Securing the Needle Position

•Step 3: Connecting the Probe to the Grounding Union

32-gauge

stainless steel needle

Tip of the 26-gauge

electrospray needle

Adapter nut

External union

and two-ferrule

assembly

5.65 in. (140 mm)

Grounding bar

of the API

source

Fingertight

fittings

Red PEEK tubing

Grounding

union

Non-conductive,

natural PEEK

internal union

Description Qty Part Number

Ferrule, 0.012 in. ID, clear 6 00101-18116

External union,

10-32, natural PEEK

2 00101-18206

Internal union,

10-32, natural PEEK

1 00109-00305

10-32 × 1/4-28, Kel-F, adapter

for sample inlet of ESI probe

1 00101-18080

High-pressure, fingertight nut

and ferrule for 1/16 in. OD

tubing

2 00101-18195

Stainless steel needle, 32-gauge,

blunt-tipped, 5.65 in. length,

0.004 in. ID × 0.009 in. OD

3 00950-00954

0.005 in. ID, red PEEK tubing 00301-22912

Teflon t ape no t sup plied

5/16 in. open-ended wrench not supplied

Internal thread

5.65 in. stainless steel needle

10-32 ×1/4-28,

Kel-F, adapter nut

Sample inlet port

in the back of the

ESI probe

Nozzle on the front

of the ESI probe

Revision E 70001-97112

3. Insert the 32-gauge stainless steel needle through the

26-gauge needle that protrudes from the nozzle of the ESI probe

(see Figure 5). Then push the needle through the probe until it

protrudes from the sample inlet at the back of the probe

(see Figure 6).

Figure 5. Inserting the stainless steel needle into the electrospray needle

Step 2: Securing the Needle Position

YTo secure the needle position

1. Slip the adapter nut over the end of the 32-gauge stainless steel

needle that protrudes from the sample inlet port. Screw the

fitting, fingertight, into the port (see Figure 6).

Figure 6. Adapter nut shown installed in the sample inlet port

2. Wrap Teflon tape around the threads on one end of the natural

PEEK external union provided in the Metal Needle Kit

(see Figure 7). Take care to avoid taping over the thru hole.

Figure 7. External union and two-ferrule assembly

3. Slide the end of the needle that protrudes from the back of the

ESI probe through the Teflon-wrapped end of the external union.

4. Screw the Teflon-wrapped end of the external union into the

adapter, fingertight.

5. Slide a clear, 0.012 in. ID ferrule over the needle that protrudes

from the back of the ESI probe.

6. To ensure that you have installed the needle correctly, check the

following:

• One end of the 32-gauge stainless steel needle is flush with

the tip of the free ferrule (see Figure 8).

• The other end of the 32-gauge stainless steel needle does not

protrude from the front of the probe by more than 1 mm.

Figure 8. Back of the ESI probe with the needle shown in the correct position

For optimal spray quality, the tip of the 32-gauge stainless steel

needle must protrude by approximately 1 mm from the tip of

the 26-gauge electrospray needle (see Figure 9).

Figure 9. Front of the ESI probe with an enlarged view of the needle tip

Step 3: Connecting the Probe to the Grounding Union

YTo connect the ESI probe to the grounding union

1. With a 5/16 in. open-ended wrench, brace the external union as

you screw the internal union made of non-conductive, natural

PEEK onto the free end of the external union (see Figure 10).

Figure 10. Connecting the internal union

2. With red PEEK tubing provided in the MS Accessory Kit and the

high-pressure fingertight fittings and ferrules provided in the

Metal Needle Kit, connect the free end of the internal union to

the grounding union held by the grounding bar (see Figure 2 on

page 1).

Trademarks

Teflon is a registered trademark of E.I. du Pont de Nemours and

Company. PEEK is a trademark of Victrex PLC. Ion Max is a

trademark of Thermo Fisher Scientific Inc.

Tip Because you must insert the 0.009 in. OD stainless steel

needle through narrow orifices, Thermo Fisher Scientific

recommends the use of a magnifying glass for this procedure.

32-gauge, 0.009 in. OD

stainless steel needle

26-gauge, 0.01 in. ID electrospray needle that

protrudes from the nozzle of the ESI probe

Needle

Adapter nut

External union, natural PEEK

End wrapped with

Teflon tape

Do not cover the

thru hole with tape.

CAUTION Only use the supplied, non-conductive,

natural PEEK union to connect the ESI probe to

the grounding union. Using a conductive union

instead of the supplied, non-conductive union can

create an electric shock hazard when the mass

spectrometer is in operation.

Needle is flush with the

tip of the ferrule.

26-gauge electrospray

needle tip

32-gauge

stainless steel

needle protruding

by 1 mm

Nozzle on

the front of the

ESI probe

Non-conductive, natural PEEK internal union

5/16 in. open-ended wrench

Thermo Fisher Scientific 32-Gauge Stainless Steel Needle Installation guide

Brand: Thermo Fisher Scientific

Model: 32-Gauge Stainless Steel Needle

Type: Installation guide

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Thermo Fisher Scientific 32-Gauge Stainless Steel Needle Installation guide

Thermo Fisher Scientific 32-Gauge Stainless Steel Needle Installation guide

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