Varian ProStar 240 Driver Manual

Brand: Varian

Model: ProStar 240

Type: Driver Manual

This manual is also suitable for

Varian, Inc.

2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA

©Varian, Inc. 2005 03-914947-21: Rev. 4

ProStar 220/230/240

Solvent Delivery Module Control

Galaxie Driver Manual

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Table of Contents

Introduction.............................................................................................. 3

General ........................................................................................................................... 3

Installation and Configuration................................................................ 4

Installing the Driver Software.......................................................................................... 4

Configuring the Communication Server.......................................................................... 4

Configuring the Driver..................................................................................................... 5

Configuring the ProStar Pump ....................................................................................5

Configuring the Synopsis ............................................................................................8

Building a Method ................................................................................. 11

Creating a New Method................................................................................................11

Control Method for the ProStar Pump........................................................................... 13

Elution Control........................................................................................................... 15

Relays Control........................................................................................................... 18

Starting and Viewing an Acquisition.................................................... 19

Quick Start and Sequence............................................................................................ 19

Quick Start (Single Acquisition).................................................................................19

Sequence .................................................................................................................. 20

Running Acquisition Status........................................................................................... 21

Viewing a Running Acquisition..................................................................................21

Status of the ProStar Pump....................................................................................... 21

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Introduction

General

This document describes the configuration procedure of the

Varian ProStar 220, 230, and 240 pump driver. It also explains

how to build a control method for this driver, how to start and

view a running acquisition and what actions are available during

an analysis.

The ProStar 220, 230 and 240 Solvent Delivery modules are

single piston pumps which deliver flow in a linear dynamic range.

The 220 is designed for isocratic (single solvent) applications

only.

Proportioned delivery of multiple solvents can be achieved by the

230 and 240 pumps. Up to 3 solvents may be run isocratically or

in gradients with the ProStar 230 SDM. The 240 SDM is a

quaternary HPLC system which allows the user to run any of four

solvents isocratically or to proportion, and run a gradient with

three solvents.

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Installation and

Configuration

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie

installation Guide.

Configuring the Communication Server

To communicate with the ProStar 220/230/240 modules a

GPIB_Interface or a NI488 communication bus must be created.

The buffer parameters of the GPIB_Interface bus to

communicate with the module must be the following:

RX Buffer =1024

TX Buffer =1024

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NOTE: The GPIB address of the pump is displayed on the rear panel of the instrument,

on a round button.

NOTE: For more details about the configuration of a GPIB_Interface or a NI488 bus,

refer to the Galaxie Chromatography Data System Installation’s Guide.

Configuring the Driver

The driver software configuration is done in the Galaxie

Configuration Manager when a new chromatographic system is

created.

Configuring the ProStar Pump

Click on the button corresponding to the ProStar pump

driver and the pump configuration screen appears.

The toolbar enables to easily scroll through the configuration

windows:

to select the first configuration window.

to select the previous configuration window.

to select the next configuration window.

to select the final configuration window.

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Communication Window

Selection will not appear

until the IEEE488 BUS is

configured.

Select from the list the name of the IEEE488 Bus previously

configured, which allows the communication between the pump

and the driver.

Give the pump module a unique GPIB address (field IEEE488

Address) corresponding to the physical one on the rear panel of

the module.

NOTE: For more details about the IEEE488 Bus configuration, refer to the section

Configuring the Communication Server in this manual.

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Hardware Window

Select from the list the Model of the Solvent Delivery module.

Three choices are available: ProStar 220 Isocratic, ProStar 230

Ternary and ProStar 240 Quaternary.

The 220 is designed for isocratic (single solvent) applications

only.

Proportioned delivery of multiple solvents can be achieved by the

230 and 240 pumps. Up to 3 solvents may be run isocratically, or

in gradients with the ProStar 230 SDM. The 240 SDM is a

quaternary HPLC system which allows the user to run any of 4

solvents isocratically, or to proportion and run a gradient with 3

solvents.

Select the option Synchronize with other modules to start the

pump when the module will receive an output signal (from

another module for example).

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Pressures Window

Select from the list the Pressure units. Three choices are

available: Bar, PSI and kPa.

Configuring the Synopsis

In the Overview part of the system configuration, a synopsis can

be defined for the system. This synopsis depends on the

configured devices.

1. To save the configuration of these devices, click on the OK

button.

2. Right click on the system name in the bottom part of the

Galaxie Configuration Manager screen. A pop-up menu

appears: click on Stop. Then right click on the system name

again, and click on Start.

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3. Go back to the System Configuration screen (Properties

wizard). A graphic of the devices that comprise the system is

displayed in the synopsis screen. To configure the synopsis

as the real instrument configuration, move each module then

connect it appropriately. To do this, select one anchor for

each of the two modules (the selected anchors become red)

and click on the

button; a connection tube will appear

between the two anchors.

Click on the

to delete a selected tube, click on the

button to delete all the tubes.

Click on

to define a background color, or on to

make the background transparent.

Click on the

icon to change the grid color.

4. Once the synopsis configuration is complete, it will be

displayed in the Galaxie Chromatography Data System, in

the Systems tab. In this tab, the Overview will show the

synopsis of the system with some parameters displayed

(temperature, flow, signal, etc.) depending on the installed

devices.

For example, the following pictures represent the synopsis of

the ProStar pump:

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Galaxie Configuration Manager Screen

Galaxie Chromatography Data System Screen

NOTE: Once the system has been created and correctly configured, it must be

associated with one or more projects in order to start acquisitions. For more

details about the creation and configuration of a system, refer to the Galaxie

Configuration Manager User’s Guide.

Press the

button to print the current configuration.

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Building a Method

Creating a New Method

The method contains parameters for instrument control, data

acquisition, chromatogram processing, and editing of final

results. To initially set up a method, it is not necessary to define

all of the sub-method sections; however, the instrument control

section must be filled in prior to starting an acquisition.

1. To create a new method, select the FILE / NEW METHOD

option from the Galaxie Chromatography Data System main

menu.

A wizard guide will appear and provide assistance during the

first steps of method creation:

2. Choose the system (chromatograph) associated with the

method. A method is created for a particular system. When

starting an acquisition, the name of the system that performs

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the acquisition must be correct, since method access is

limited only to those methods associated with this system.

3. Once this field is completed, click on the Next button to

move to the next step of the method creation:

4. Enter the New Method Name in the first field. In the

Description field, enter information concerning the method

(optional).

5. Once these two fields have been completed, click on the OK

button. The new method is created and is opened with

default parameters in the Galaxie Chromatography Data

System.

The next step is the definition of each of the method

sections.

The instrument control method section must be defined in

order to start an acquisition. In the following part of this

guide, only the control section of the method for the ProStar

220, 230, and 240 pumps is detailed. Other parameters such

as Injection Volume, Vial and Rack number could be

necessary. They are defined in the acquisition sub-method

part.

NOTE: For more details about the programming of each method section, refer to the

Galaxie Chromatography Data System User’s Guide.

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Control Method for the ProStar Pump

Once the new method is created, select the control section in the

lower part of the browser.

The following screen appears:

Press the

button to display the System Control Method

Advanced Tools window:

to send the current method to the instrument (not

applicable to this driver)

to get the active method from the instrument (not

applicable to this driver)

to print the current method

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Click on the button to display the ProStar pump control

method section. It contains two sub-sections: Elution and Relays.

Click on the required sub-section to define the corresponding

parameters that will be used during the chromatographic run.

The toolbar enables to easily scroll through the control method

windows:

to select the first window of the control method.

to move back to the previous window of the control

method.

to move forward to the next window of the control

method.

to select the final window of the control method

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Elution Control

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Select from the list(s) the name of each solvent. Here are listed

all the available solvents:

Water Acetone

Acetonitrile Benzene

Carbon tetrafluoride Chloroform

Cyclohexane Dichloroethane

Diethyl Ether Dimethylformamide

Dioxane Ethanol

Ethyl Acetate Methylene Chloride

Methanol n-heptane

n-hexane o-dichlorobenzene

Propanol Tetrahydrofuran

Toluene 2-Methylformamide

The activation of the scrolling lists A, B, C depends on the model

of the pump previously configured in the Galaxie Configuration

Manager (isocratic, ternary or quaternary pump).

Enter the Equilibration time in the corresponding field.

A programmed equilibration is intended to cause a smooth

changeover in solvent composition in automated applications,

and is executed at the end of the run or when a new method with

a different flow rate or composition than the last conditions is

activated. Equilibration is not done if the current conditions are

the same as the T=0 conditions or the Equilibration value is set

to 0.

During this equilibration period a gradient change from the

current solvent composition and flow rate to the Time=0

composition and flow rate occurs. The gradient composition

change takes 10% of the equilibration time, with the new

conditions being held for the remaining 90% of the time.

Enter the Run time of the pump in the corresponding field. This

parameter allows the gradient to continue after the acquisition is

finished.

Program a flow and/or a composition gradient. Each step of the

program includes time, flow and/or composition new values. The

Pumps Gradient Chart represents the progression of these

parameters.

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Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

End of analysis behavior:

In the scrolling list, select either to stop the pump or leave the

pump on at the end of the analysis (Quick Start or sequence).

If Hold is selected, the pump will continue to work at the last

time-table flow and composition.

If Equilibrate is selected, the pump will perform an equilibration

step to return to the initial conditions and will continue to work at

the initial conditions.

If Stop Pump (end of analysis) is selected, the pump will stop at

the end of each run.

If Stop Pump (end of sequence only), the pump will stop only

when the sequence is finished.

Enter in the corresponding fields the minimum and maximum

pressure limits. The pressure range is 0 to 410 Bar, (0 to 5946

PSI, and 0 to 40996 kPa).

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Relays Control

This type of pump has six external event relays which may be

time programmed. Each step of the program includes time and

state values for each relay.

NOTE: The relay 1 is used to select between the two positions of a low pressure solvent

switching valve connected to the “A” proportioning valve in case of 240 SDM

quaternary HPLC system. This allows the user to run any of 4 solvents

isocratically, or to proportion a gradient with 3 solvents.

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

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Starting and Viewing an

Acquisition

Quick Start and Sequence

Once the instrument control method has been correctly

programmed, an analysis can be started. The next section briefly

describes how to start an acquisition.

Quick Start (Single Acquisition)

1. Press the button

In the Quick Start window, select the system, the method,

and optionally the project if connected in "All project" mode.

2. Press the OK button. The following screen is displayed:

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In the Acquisition parameters tab, requiered fields like the

Run Name, the Run ID, and the Acquisition length must be

filled in before starting an analysis. Furthermore, if the

system is equipped with an autosampler controlled by

Galaxie Chromatography Data System, the Injection

Volume, the Vial position and the Rack number (if required)

must be entered.

Other parameters appropriate to each acquisition channel

can be entered in the Channel X tab.

3. Click on the Start button to start the acquisition if the system

is a single-injector system or click on the Next button if the

system is a multiple injector system. In a multiple injector

system, the same parameters entered for the first injector

must be entered for the other injectors before starting the

acquisition.

Sequence

1. To create a sequence slect the FILE / NEW SEQUENCE

menu and complete the displayed creation wizard screen

(refer to the Galaxie Chromatography Data System User’s

Guide), by entering the system name that will perform the

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Varian ProStar 240 Driver Manual

Brand: Varian

Model: ProStar 240

Type: Driver Manual

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Varian ProStar 240 Driver Manual

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