Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
©Varian, Inc. 2005 03-914947-20:Rev. 5
ProStar 210/215 and PrepStar
218/SD-1 Solvent Delivery
Module Control
Galaxie Driver Manual
ProStar 210/215/ PrepStar 218/SD-1 SDM -2- 03-914947-20:5
Table of Contents
Introduction.............................................................................................. 3
General ........................................................................................................................... 3
Installation and Configuration................................................................ 4
Installing the Driver Software .......................................................................................... 4
Configuring the Communication Server.......................................................................... 4
Configuring the Driver ..................................................................................................... 5
Configuring the ProStar / PrepStar Pump................................................................... 5
Configuring the Synopsis ............................................................................................9
Building a Method ................................................................................. 12
Creating a New Method ................................................................................................ 12
ProStar / PrepStar Pump Control Method .................................................................... 14
Elution Control........................................................................................................... 16
Miscellaneous Control............................................................................................... 18
Pressure Control........................................................................................................19
Injection Control.........................................................................................................20
Starting and Viewing an Acquisition.................................................... 22
Quick Start and Sequence ............................................................................................ 22
Quick Start (Single Acquisition).................................................................................22
Sequence .................................................................................................................. 23
Running Acquisition Status ........................................................................................... 24
Viewing a Running Acquisition..................................................................................24
Status of the ProStar / PrepStar Pump ..................................................................... 24
Appendix................................................................................................ 30
Pressure Limits (in bar)................................................................................................. 30
Compressibility and Pressure Constant........................................................................ 31
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Introduction
General
This document describes the configuration of the Varian ProStar
210, 215 and Varian PrepStar 218, SD-1 pump driver. It also
explains how to build a control method for this driver, how to start
and view a running acquisition and what actions are available
during an analysis.
The ProStar 210, 215 and PrepStar 218 Solvent Delivery
Modules are identical in appearance and operation. The ProStar
215 and PrepStar 218 have more powerful motors, larger power
supplies and can operate at higher pressures than the ProStar
210. The pressures are identified in the Appendix.
The manual will refer to the pumps as 210/215/218/SD-1
throughout.
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Installation and
Configuration
Installing the Driver Software
To install the Galaxie Drivers software refer to the Galaxie Data
System Installation Guide.
Configuring the Communication Server
To communicate with the ProStar 210-215-218-SD-1 module a
RS232_Interface or a RS232_PC communication bus must be
configured on the acquisition server.
The parameters of the bus to must be the following:
Stops
1 Stop
Baud 19200
Parity EVEN
Bits 8 Bits
Flow Control NONE
Buffer Tx 4096
Buffer Rx 4096
NOTE: For more details about the configuration of a RS232_Interface or a RS232_PC
bus, refer to the Galaxie Chromatography Data System Installation’s Guide.
ProStar 210/215/ PrepStar 218/SD-1 SDM -5- 03-914947-20:5
Configuring the Driver
The driver software configuration is done in the Galaxie
Configuration Manager when a new chromatographic system is
created.
Configuring the ProStar / PrepStar Pump
Click on the button corresponding to the ProStar /
PrepStar pump driver and the pump configuration screen
appears:
The toolbar allows you to easily scroll through the configuration
windows:
to select the first configuration window.
to select the previous configuration window.
to select the next configuration window.
to select the final configuration window.
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Communications Window
Select from the list the name of the RS422 Bus where the
system has been connected.
NOTE: For more details about the RS422 Bus configuration, refer to the section
Configuring the Communication Server in this manual.
Selection will not appear
until the RS422 BUS is
configured.
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Hardware Window
Select from the list the type of the pump: isocratic, binary,
ternary, quaternary, isocratic + injector, binary + injector, ternary
+ injector. A new tab (Pump B, Pump C, Pump D or Injector)
appears depending on the selected type of pump.
NOTE:
- The system must be equipped by at least two ProStar / PrepStar modules to work as a
binary pump, three modules for a ternary one and four to work in quaternary mode.
- With this type of pump, a module can be used as an injector. In this case a new
Injection screen becomes active in the method. To learn more about this option, refer to
the chapter
Control method for the ProStar / PrepStar pump.
- When an Injector module is configured, in the lower part of the Galaxie Configuration
Manager an injector (of the new system) called ProStar 210/215/218 pump is created.
The ID entered here
must match the ID of
the pump.
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Enter the identification number of the pump module in the RS-
422 unit ID field. The RS-422 unit ID field must match the ID
specified on the pump itself. To check the ID on the pump, press
Setup on the pump (no flow), scroll left until the ID is displayed.
Press Enter when finished.
Select from the list the model of the pump module. Four choices
are available: ProStar 210, 215, PrepStar 218 and SD-1.
The 210/215/218 modules are identical in appearance and
operation. They differ only in one respect: the ProStar 215 and
PrepStar 218 have more powerful motors, larger power supplies
and can operate at higher pressures than the ProStar 210.
NOTE: The pump head size must be configured on the instrument. Maximum flow rates
authorized in Galaxie will then depend on that configuration.
- With ProStar 210/215 and PrepStar 218: press the ~ button to access the setup menu
and enter the correct head size under Hdsz section using the ▲ and ▼ buttons.
- With the PrepStar SD-1: press the ~ button to access the setup menu. Select YES
under Change Pump using the ▲ and ▼ buttons and follow the procedure displayed in
the LCD screen. At the end of the procedure, new pump head size will be automatically
configured.
Please refer to the instrument manual for more details.
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Pressures Window
Select from the list the Pressure units. Three choices are
available: bar, PSI and kPa.
Configuring the Synopsis
In the Overview part of the system configuration, a synopsis can
be defined for the system. This synopsis depends on the
configured devices.
1. To save the configuration of these devices, click on the OK
button.
2. Right click on the system name in the bottom part of the
Galaxie Configuration Manager screen. A pop-up menu
appears: click on Stop. Then right click on the system name
again, and click on Start.
ProStar 210/215/ PrepStar 218/SD-1 SDM -10- 03-914947-20:5
3. Go back to the System Configuration screen (Properties
wizard). A graphic of the devices that comprise the system is
displayed in the synopsis screen. To configure the synopsis
as the real instrument configuration, move each module, and
then connect it appropriately. To do this, select one anchor
for each of the two modules (the selected anchors become
red) and click on the
button; a connection tube will
appear between the two anchors.
Click on the
button to delete a selected tube, click on
the
button to delete all the tubes.
Click on
to define a background color, or on to
make the background transparent.
Click on the
icon to change the grid color.
4. Once the synopsis configuration is correctly done, it will be
displayed in Galaxie Chromatography Data System, in the
Systems tab. In this tab, the Overview part will show the
synopsis of the system with some parameters displayed
(temperature, flow, signal, etc.) depending on the installed
devices.
For example, the following pictures represent the synopsis of
the ProStar / PrepStar pump:
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Galaxie Configuration Manager Screen
Galaxie Chromatography Data System Screen
NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start acquisitions. For more
details about the creation and configuration of a system, refer to the Galaxie
Configuration Manager User’s Guide (chapter The System).
Click on the
button to print the current configuration
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Building a Method
Creating a New Method
The method contains parameters for instrument control, data
acquisition, chromatogram processing, and editing of final
results. To initially set up a method, it is not necessary to define
all of the sub-method sections; however, you must fill in the
instrument control section prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD
option from the Galaxie Chromatography Data System main
menu.
A wizard guide is displayed to provide assistance during the
first steps of method creation:
2. Choose the system (chromatograph) associated with the
method. A method is created for a particular system. When
ProStar 210/215/ PrepStar 218/SD-1 SDM -13- 03-914947-20:5
starting an acquisition, the name of the system that performs
the acquisition must be correct, since method access is
limited only to those methods associated with this system.
3. Once this field is completed click on the Next button to move
to the second step of the method creation:
4. Enter the New Method Name in the first field. In the
Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the OK
button. The new method is created and is opened with
default parameters in the Galaxie Chromatography Data
System. The next step is the definition of each of the method
sections.
The instrument control section must be defined in order to
start an acquisition. In the following part of this guide, only
the instrument control section of the method for the ProStar /
PrepStar pump is detailed. Other parameters such as
Injection Volume, Vial and Rack number could be necessary.
They are defined in the acquisition sub-method part.
NOTE: For more details about the programming of each method section, refer to the
Galaxie Chromatography Data System User’s Guide.
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ProStar / PrepStar Pump Control Method
Once the new method is created, select the control section in the
lower part of the browser.
The following screen appears:
ProStar 210/215/ PrepStar 218/SD-1 SDM -15- 03-914947-20:5
Press the button to display the System Control Method
Advanced Tools window:
to send the current method to the instrument (not
applicable to this driver)
to get the active method from the instrument (not
applicable to this driver)
to print the current method
Click on the button to display the ProStar / PrepStar
pump control method section. It contains up to four sub-sections:
Elution, Miscellaneous, Pressure and Injection. Click on the
required sub-section to define the corresponding parameters that
will be used during the chromatographic run. The toolbar allows
you to easily scroll through the control method windows:
to select the first window of the control method.
to move back to the previous window of the control
method.
to move forward to the next window of the control
method.
to select the final window of the control method.
ProStar 210/215/ PrepStar 218/SD-1 SDM -16- 03-914947-20:5
Elution Control
Select from the list(s) the solvent of each pump module. Here
are listed all the available solvents:
Water Acetone
Acetonitrile Benzene
Carbon tetrafluoride Chloroform
Cyclohexane Dichloroethane
Diethyl Ether Dimethylformamide
Dioxane Ethanol
Ethyl Acetate Methylene Chloride
Methanol n-heptane
n-hexane o-dichlorobenzene
Propanol Tetrahydrofuran
Toluene 2-Methylformamide
ProStar 210/215/ PrepStar 218/SD-1 SDM -17- 03-914947-20:5
A compressibility factor and a pressure constant are associated
with each solvent (Compressibility and Pressure Constant fields).
When the Compressibility compensation field is unchecked, the
pump will not take into account the corresponding factors and
the factors will be concealed.
Refer to the Compressibility and Pressure Constant section in
the appendix of this manual to find the list of these values.
The refill time parameter is the total time that it takes to refill the
pump head with mobile phase. This value may be changed
when a very viscous mobile phase is used. In this case, a rapid
refill might lead to cavitations or bubble formation in the pump
head causing a lower than expected flow rate. If the refill time is
too long, excessive pulsations in the flow may be observed. This
parameter is programmable by pump module.
NOTE: The activation of the scrolling lists A, B…D depends on the type of the pump
previously configured in the Galaxie Configuration Manager (isocratic, binary,
ternary or quaternary pump).
Program a flow and/or a composition gradient. Each step of the
program includes time, flow and/or composition new values. The
Pumps Gradient Chart represents the evolution of these
parameters.
Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.
Below the pump gradient chart, select the display type of the
chart. Two choices are available Solvent A on top or Solvent A
on bottom.
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Miscellaneous Control
Start mode:
In the scrolling list, select the way to start the pump.
If immediately when ready is chosen, the pump will start as soon
as it is ready.
If inject trigger on pump X is chosen, the pump will start when an
inject marker is send to pump X.
End of Sequence:
In the scrolling list, select either to stop or not the pump at the
end of the analysis (Quick Start or sequence).
If Leave Pump On is selected, the pump will continue to work at
the last time-table programmed flow and composition.
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Pressure Control
Pressure Limits Parameter Group:
Enter the minimum and maximum pressure limits of the pump.
Outside these limits, the pump will stop and an alarm will sound.
NOTE:
- The maximum pressure limit of a ProStar / PrepStar module depends on the type of its
pump head. This limit is automatically adapted according to the connected pump head.
To know these limits, refer to the Pressure Limits chapter in the appendix of this manual.
- If a higher value of pressure is entered by error, a message appears indicating the
pressure limits for the connected pump head and the last entered valid value is
displayed.
- If several ProStar / PrepStar modules are connected (in case of binary, ternary or
quaternary pump), the maximum pressure limit is the lowest value among the different
pump ones.
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Injection Control
This window becomes active only when a pump module is
configured as an injector in the system (for more details refer to
the Configuration of the ProStar / PrepStar pump chapter).
Thru Pump Injection Parameter Group:
Enter the time when the injection will start in the Injection time
field.
Enter the flow rate of the injection in the Injection Rate field. The
end time of the injection is determined according to the flow rate
and the injection volume entered in the Acquisition method
section (or in the Quick Start window or in the Sequence).
NOTE: The Injection Volume entered in the Acquisition part of the method must be
entered in mL only.
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