Varian ProStar 325 UV-Vis Driver Manual

Brand: Varian

Model: ProStar 325 UV-Vis

Type: Driver Manual

Varian, Inc.

2700 Mitchell Drive

Walnut Creek, CA 94598-1675/USA

©Varian, Inc. 2005 03-914947-44:Rev. 2

ProStar 325 UV-Vis

Detector Control

Galaxie Driver Manual

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Table of Contents

Introduction.............................................................................................. 2

General................................................................................................................................2

Installation and Configuration................................................................ 3

Installing the Driver Software...............................................................................................3

Varian ProStar 325 Communications Configuration............................................................3

Ethernet Communications.............................................................................................3

Configuring the Driver..........................................................................................................4

Configuring the Varian ProStar 325 UV-Vis Detector...................................................4

Configuring the Synopsis..............................................................................................8

Building a Method ................................................................................. 10

Creating a New Method.....................................................................................................10

Varian ProStar 325 UV-Vis Detector Control Method .......................................................12

Signal Control..............................................................................................................14

Peak Sensor Control...................................................................................................16

Relay Control ..............................................................................................................18

Miscellaneous Control.................................................................................................19

Starting and Viewing an Acquisition.................................................... 21

Quick Start and Sequence.................................................................................................21

Quick Start (Single Acquisition) ..................................................................................21

Sequence....................................................................................................................23

Running Acquisition Status................................................................................................24

Viewing a Running Acquisition....................................................................................24

Varian ProStar 325 UV-Vis Detector Status ...............................................................24

Appendix................................................................................................ 28

Peak Sensor Functions .....................................................................................................28

Time Slice Function.....................................................................................................28

Pulse Relay Function..................................................................................................29

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Introduction

General

This document describes the configuration of the Varian

ProStar 325 UV-Vis Detector driver. It also explains how to

build a control method for this driver, how to start and view a

running acquisition, and what actions are available during an

analysis.

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Installation and Configuration

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie

installation Guide.

Varian ProStar 325 Communications Configuration

Ethernet Communications

The Varian ProStar 325 UV-Vis Detector uses Ethernet as the

method of communications for instrument control.

The Varian ProStar 325 UV-Vis Detector uses TCP/IP

(Transmission Control Protocol/Internet Protocol) to

communicate over the Ethernet network and needs to be given

a unique IP address to identify the instrument. A static IP

address can be also configured in the Varian ProStar 325 with

the handheld unit.

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A BOOTP Server is used to send IP addresses to TCP/IP

devices.

The BOOTP Server lists Ethernet addresses along with the IP

addresses that are to be assigned to the corresponding

devices.

To known how to configure the BOOTP server, refer to the

Galaxie installation Guide.

NOTE: The BOOTP server provided by the Galaxie Chromatography Data System is

installed during the installation of the Galaxie software.

Configuring the Driver

The driver software configuration is done in the Galaxie

Configuration Manager when a new chromatographic system is

created.

Configuring the Varian ProStar 325 UV-Vis Detector

Click on the button corresponding to the Varian ProStar

325 driver and the configuration screen appears.

The toolbar allows you to easily scroll through the configuration

windows:

to select the first configuration window.

to select the previous configuration window.

to select the next configuration window.

to select the final configuration window.

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Communication Window

In the IP Address field, enter the IP address of the instrument.

Press the button

to search the subnet for all connected

Varian ProStar 325 instruments. The following window will be

displayed.

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Once an IP Address is configured, press the button

to activate the communication with the

instrument.

Check the Synchronize with other modules box to enable this

option. When this option is enabled the Varian ProStar 325 will

be started from a contact closure which will be able to receive a

fault signal directly from another instrument.

Check the Enable Ready In box to enable this option. When this

option is enabled the Varian ProStar 325 waits for a ready signal

to go to the ready state.

NOTE: For more details about the BOOTP configuration, refer to the Ethernet

Communications section.

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Option Window

Press the

button to enter a code which

will enable the Dual Wavelength mode in the instrument. Then

check or uncheck the Two Wavelength Option to use it in

Galaxie.

Select the Cell Type according to the one physically installed in

the detector, four cell types are selectable: the 9x0, 9x1, 4x0

and 4x0.15.

If a prep flow cell is selected, the user will be able to enter a

Cell Ratio. The ratio is used for extended range operation. The

ratio for each flow cell is determined at the factory and is

included with the flow cell. The detector will always operate in

extended range mode with a prep flow cell.

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Configuring the Synopsis

In the Overview part of the system configuration, a synopsis can

be defined for the system. This synopsis depends on the

configured devices.

1. To save the configuration of these devices, click on the OK

button.

2. Right-click on the system name in the bottom part of the

Galaxie Configuration Manager screen. A pop-up menu

appears: click on Stop. Then right-click on the system name

again, and click on Start.

3. Go back to the System Configuration screen (Properties

wizard). A graphic of the devices that comprise the system

is displayed in the synopsis screen. To configure the

synopsis as the real instrument configuration, move each

module then connect it appropriately. To do this, select one

anchor for each of the two modules (the selected anchors

become red) and click on the

button; a connection

tube will appear between the two anchors.

Click on the

to delete a selected tube; click on the

button to delete all the tubes.

Click on

to define a background color, or on to

make the background transparent.

Click on the

icon to change the grid color.

4. Once the synopsis configuration is complete, it will be

displayed in the Galaxie Chromatography Data System, in

the Systems tab. In this tab, the Overview part will show the

synopsis of the system with some parameters displayed

(temperature, flow, signal, etc.) depending on the installed

devices.

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For example, the following pictures represent the synopsis

of the Varian ProStar 325:

Galaxie Configuration Manager Screen

Galaxie Chromatography Data System Screen

NOTE: Once the system has been created and correctly configured, it must be

associated with one or more projects in order to start some acquisitions. For

more details about the creation and configuration of a system, refer to the

Galaxie Configuration Manager User’s Guide.

Click on the

button to print the current configuration.

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Building a Method

Creating a New Method

The method contains parameters for instrument control, data

acquisition, chromatogram processing, and editing of final

results. To initially set up a method, it is not necessary to define

all of the sub-method sections; however, you must fill in the

instrument control section prior to starting an acquisition.

1. To create a new method, select the FILE / NEW METHOD

option from the Galaxie Chromatography Data System main

menu.

A wizard guide will appear and provide assistance during

the first steps of method creation:

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2. Choose the system (chromatograph) associated with the

method. A method is created for a particular system. When

starting an acquisition, the name of the system that

performs the acquisition must be correct, since method

access is limited only to those methods associated with this

system.

3. Once this field is completed, click on the Next button to

move to the second step of the method creation:

4. Enter the New Method Name in the first field. In the

Description field, enter information concerning the method

(optional).

5. Once these two fields have been completed, click on the

OK button. The new method is created and is opened with

default parameters in the Galaxie Chromatography Data

System. The next step is the definition of each of the

method sections.

The instrument control section must be defined in order to

start an acquisition. In the following part of this guide, only

the instrument control section for the Varian ProStar 325

UV-Vis Detector is detailed. Other parameters such as

Injection Volume, Vial and Rack number may be necessary

and are defined in the acquisition section.

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NOTE: For more details about the programming of each method section, turn to the

Galaxie Chromatography Data System User’s Guide.

Varian ProStar 325 UV-Vis Detector Control Method

Once the new method is created, select the control section in

the lower part of the browser.

The following screen appears:

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Press the button to display the System Control Method

Advanced Tools window:

to send the current method to the instrument.

to get the active method from the instrument.

to print the current method.

Click on the

button to display the Varian ProStar 325

control method sections. It contains four sub-sections: Signal,

Peak Sensor, Relay and Miscellaneous. Click on the required

sub-section to define the corresponding parameters that will be

used during the chromatographic run. The toolbar allows you to

easily scroll through the control method windows:

to select the first window of the control method.

to move back to the previous window of the control

method.

to move forward to the next window of the control method.

to select the final window of the control method.

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Signal Control

Select from the list the Bunch Rate of the detector. With

increased bunch rates, less disk space is required to store the

data.

With decreased bunch rates, more data is collected. Bunch rate

should be set appropriately for the narrowest peak of interest in

the chromatogram.

Enter the Noise Monitor Length in the corresponding field. This

parameter sets the number of points collected during the

monitoring period and is used to calculate the baseline noise.

Enter the Response Time in the corresponding field. This

parameter specifies the degree of electronic noise filtering

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applied to the analog outputs. It affects only the recorder and

data system analog outputs from the detector module, and does

not affect the data sent to the Galaxie Chromatography Data

System.

Enter the time program for the Wavelength, the Attenuation, and

the Autozero parameters.

The attenuation parameter switches the detector's output

attenuation to one of the eleven available settings. Attenuation is

measured in Absorbance Units Full Scale (AUFS). This affects

only the recorder’s output.

If Yes is selected in the Autozero field, the detector’s output is

automatically zeroed. This affects both the chromatogram display

and the data acquired for permanent storage.

Each step of the program includes time, wavelength, attenuation,

and autozero values.

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

If Dual Wavelength Mode is checked extra columns are added in

the table which will allow the user to enter another Wavelength,

Attenuation and an Autozero value.

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Peak Sensor Control

Enter the time program for the Peak Sensor parameters.

In the Peak Relay column you can activate the Time Slice

function. Time slicing allows the Peaksense relay to be actuated

at regularly spaced intervals during the run. The time slicing can

be turned on or off (field at specified times, for example, On Time

2.5 min, Off Time 12.7 min. This parameter cannot be set at

initial conditions (time 0.00).

The Period parameter is the time between Peaksense relay

actuations during a time slice event. A relay period of 30 causes

the Peaksense relay to actuate every 30 seconds while time

slicing is turned on.

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In the Peak Relay column you can also activate the Pulse Relay

function The Pulse relay activates or deactivates the Peaksense

relay at the specified time. This parameter cannot be set at initial

conditions (time 0.00).

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

NOTE: For more details about the Peak Sensor functions (Timeslice or Pulse), refer to

the appendix (Peak Sensor functions section) at the end of this manual.

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Relay Control

This detector has four external event relays which may be time

programmed. Each step of the program includes time and state

for each relay’s new values.

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

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Miscellaneous Control

Analog Control Group:

In the Analog X Source scrolling list, select Wavelength 1,

Wavelength 2, or none. These entries specify which signal will be

chosen for the two analog outputs on the rear panel of the

detector. Wavelength 2 is only useful if the detector is operating

in dual channel mode. The same signal can be output from the

two analog channels even if the detector is operating in single

wavelength mode.

In the Analog X Peak Ticks scrolling list, select enable or disable.

Peak Ticks are small spikes added to the analog output to

indicate when the Peak relay is activated.

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Varian ProStar 325 UV-Vis Driver Manual

Varian ProStar 325 UV-Vis Driver Manual

Varian ProStar 325 UV-Vis Driver Manual

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