Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
©Varian, Inc. 2005 03-914947-21: Rev. 4
ProStar 220/230/240
Solvent Delivery Module Control
Galaxie Driver Manual
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Table of Contents
Introduction.............................................................................................. 3
General ........................................................................................................................... 3
Installation and Configuration................................................................ 4
Installing the Driver Software.......................................................................................... 4
Configuring the Communication Server.......................................................................... 4
Configuring the Driver..................................................................................................... 5
Configuring the ProStar Pump ....................................................................................5
Configuring the Synopsis ............................................................................................8
Building a Method ................................................................................. 11
Creating a New Method................................................................................................11
Control Method for the ProStar Pump........................................................................... 13
Elution Control........................................................................................................... 15
Relays Control........................................................................................................... 18
Starting and Viewing an Acquisition.................................................... 19
Quick Start and Sequence............................................................................................ 19
Quick Start (Single Acquisition).................................................................................19
Sequence .................................................................................................................. 20
Running Acquisition Status........................................................................................... 21
Viewing a Running Acquisition..................................................................................21
Status of the ProStar Pump....................................................................................... 21
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Introduction
General
This document describes the configuration procedure of the
Varian ProStar 220, 230, and 240 pump driver. It also explains
how to build a control method for this driver, how to start and
view a running acquisition and what actions are available during
an analysis.
The ProStar 220, 230 and 240 Solvent Delivery modules are
single piston pumps which deliver flow in a linear dynamic range.
The 220 is designed for isocratic (single solvent) applications
only.
Proportioned delivery of multiple solvents can be achieved by the
230 and 240 pumps. Up to 3 solvents may be run isocratically or
in gradients with the ProStar 230 SDM. The 240 SDM is a
quaternary HPLC system which allows the user to run any of four
solvents isocratically or to proportion, and run a gradient with
three solvents.
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Installation and
Configuration
Installing the Driver Software
To install the Galaxie Drivers software refer to the Galaxie
installation Guide.
Configuring the Communication Server
To communicate with the ProStar 220/230/240 modules a
GPIB_Interface or a NI488 communication bus must be created.
The buffer parameters of the GPIB_Interface bus to
communicate with the module must be the following:
RX Buffer =1024
TX Buffer =1024
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NOTE: The GPIB address of the pump is displayed on the rear panel of the instrument,
on a round button.
NOTE: For more details about the configuration of a GPIB_Interface or a NI488 bus,
refer to the Galaxie Chromatography Data System Installation’s Guide.
Configuring the Driver
The driver software configuration is done in the Galaxie
Configuration Manager when a new chromatographic system is
created.
Configuring the ProStar Pump
Click on the button corresponding to the ProStar pump
driver and the pump configuration screen appears.
The toolbar enables to easily scroll through the configuration
windows:
to select the first configuration window.
to select the previous configuration window.
to select the next configuration window.
to select the final configuration window.
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Communication Window
Selection will not appear
until the IEEE488 BUS is
configured.
Select from the list the name of the IEEE488 Bus previously
configured, which allows the communication between the pump
and the driver.
Give the pump module a unique GPIB address (field IEEE488
Address) corresponding to the physical one on the rear panel of
the module.
NOTE: For more details about the IEEE488 Bus configuration, refer to the section
Configuring the Communication Server in this manual.
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Hardware Window
Select from the list the Model of the Solvent Delivery module.
Three choices are available: ProStar 220 Isocratic, ProStar 230
Ternary and ProStar 240 Quaternary.
The 220 is designed for isocratic (single solvent) applications
only.
Proportioned delivery of multiple solvents can be achieved by the
230 and 240 pumps. Up to 3 solvents may be run isocratically, or
in gradients with the ProStar 230 SDM. The 240 SDM is a
quaternary HPLC system which allows the user to run any of 4
solvents isocratically, or to proportion and run a gradient with 3
solvents.
Select the option Synchronize with other modules to start the
pump when the module will receive an output signal (from
another module for example).
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Pressures Window
Select from the list the Pressure units. Three choices are
available: Bar, PSI and kPa.
Configuring the Synopsis
In the Overview part of the system configuration, a synopsis can
be defined for the system. This synopsis depends on the
configured devices.
1. To save the configuration of these devices, click on the OK
button.
2. Right click on the system name in the bottom part of the
Galaxie Configuration Manager screen. A pop-up menu
appears: click on Stop. Then right click on the system name
again, and click on Start.
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3. Go back to the System Configuration screen (Properties
wizard). A graphic of the devices that comprise the system is
displayed in the synopsis screen. To configure the synopsis
as the real instrument configuration, move each module then
connect it appropriately. To do this, select one anchor for
each of the two modules (the selected anchors become red)
and click on the
button; a connection tube will appear
between the two anchors.
Click on the
to delete a selected tube, click on the
button to delete all the tubes.
Click on
to define a background color, or on to
make the background transparent.
Click on the
icon to change the grid color.
4. Once the synopsis configuration is complete, it will be
displayed in the Galaxie Chromatography Data System, in
the Systems tab. In this tab, the Overview will show the
synopsis of the system with some parameters displayed
(temperature, flow, signal, etc.) depending on the installed
devices.
For example, the following pictures represent the synopsis of
the ProStar pump:
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Galaxie Configuration Manager Screen
Galaxie Chromatography Data System Screen
NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start acquisitions. For more
details about the creation and configuration of a system, refer to the Galaxie
Configuration Manager User’s Guide.
Press the
button to print the current configuration.
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Building a Method
Creating a New Method
The method contains parameters for instrument control, data
acquisition, chromatogram processing, and editing of final
results. To initially set up a method, it is not necessary to define
all of the sub-method sections; however, the instrument control
section must be filled in prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD
option from the Galaxie Chromatography Data System main
menu.
A wizard guide will appear and provide assistance during the
first steps of method creation:
2. Choose the system (chromatograph) associated with the
method. A method is created for a particular system. When
starting an acquisition, the name of the system that performs
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the acquisition must be correct, since method access is
limited only to those methods associated with this system.
3. Once this field is completed, click on the Next button to
move to the next step of the method creation:
4. Enter the New Method Name in the first field. In the
Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the OK
button. The new method is created and is opened with
default parameters in the Galaxie Chromatography Data
System.
The next step is the definition of each of the method
sections.
The instrument control method section must be defined in
order to start an acquisition. In the following part of this
guide, only the control section of the method for the ProStar
220, 230, and 240 pumps is detailed. Other parameters such
as Injection Volume, Vial and Rack number could be
necessary. They are defined in the acquisition sub-method
part.
NOTE: For more details about the programming of each method section, refer to the
Galaxie Chromatography Data System User’s Guide.
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Control Method for the ProStar Pump
Once the new method is created, select the control section in the
lower part of the browser.
The following screen appears:
Press the
button to display the System Control Method
Advanced Tools window:
to send the current method to the instrument (not
applicable to this driver)
to get the active method from the instrument (not
applicable to this driver)
to print the current method
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Click on the button to display the ProStar pump control
method section. It contains two sub-sections: Elution and Relays.
Click on the required sub-section to define the corresponding
parameters that will be used during the chromatographic run.
The toolbar enables to easily scroll through the control method
windows:
to select the first window of the control method.
to move back to the previous window of the control
method.
to move forward to the next window of the control
method.
to select the final window of the control method
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Elution Control
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Select from the list(s) the name of each solvent. Here are listed
all the available solvents:
Water Acetone
Acetonitrile Benzene
Carbon tetrafluoride Chloroform
Cyclohexane Dichloroethane
Diethyl Ether Dimethylformamide
Dioxane Ethanol
Ethyl Acetate Methylene Chloride
Methanol n-heptane
n-hexane o-dichlorobenzene
Propanol Tetrahydrofuran
Toluene 2-Methylformamide
The activation of the scrolling lists A, B, C depends on the model
of the pump previously configured in the Galaxie Configuration
Manager (isocratic, ternary or quaternary pump).
Enter the Equilibration time in the corresponding field.
A programmed equilibration is intended to cause a smooth
changeover in solvent composition in automated applications,
and is executed at the end of the run or when a new method with
a different flow rate or composition than the last conditions is
activated. Equilibration is not done if the current conditions are
the same as the T=0 conditions or the Equilibration value is set
to 0.
During this equilibration period a gradient change from the
current solvent composition and flow rate to the Time=0
composition and flow rate occurs. The gradient composition
change takes 10% of the equilibration time, with the new
conditions being held for the remaining 90% of the time.
Enter the Run time of the pump in the corresponding field. This
parameter allows the gradient to continue after the acquisition is
finished.
Program a flow and/or a composition gradient. Each step of the
program includes time, flow and/or composition new values. The
Pumps Gradient Chart represents the progression of these
parameters.
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Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.
End of analysis behavior:
In the scrolling list, select either to stop the pump or leave the
pump on at the end of the analysis (Quick Start or sequence).
If Hold is selected, the pump will continue to work at the last
time-table flow and composition.
If Equilibrate is selected, the pump will perform an equilibration
step to return to the initial conditions and will continue to work at
the initial conditions.
If Stop Pump (end of analysis) is selected, the pump will stop at
the end of each run.
If Stop Pump (end of sequence only), the pump will stop only
when the sequence is finished.
Enter in the corresponding fields the minimum and maximum
pressure limits. The pressure range is 0 to 410 Bar, (0 to 5946
PSI, and 0 to 40996 kPa).
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Relays Control
This type of pump has six external event relays which may be
time programmed. Each step of the program includes time and
state values for each relay.
NOTE: The relay 1 is used to select between the two positions of a low pressure solvent
switching valve connected to the “A” proportioning valve in case of 240 SDM
quaternary HPLC system. This allows the user to run any of 4 solvents
isocratically, or to proportion a gradient with 3 solvents.
Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.
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Starting and Viewing an
Acquisition
Quick Start and Sequence
Once the instrument control method has been correctly
programmed, an analysis can be started. The next section briefly
describes how to start an acquisition.
Quick Start (Single Acquisition)
1. Press the button
In the Quick Start window, select the system, the method,
and optionally the project if connected in "All project" mode.
2. Press the OK button. The following screen is displayed:
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In the Acquisition parameters tab, requiered fields like the
Run Name, the Run ID, and the Acquisition length must be
filled in before starting an analysis. Furthermore, if the
system is equipped with an autosampler controlled by
Galaxie Chromatography Data System, the Injection
Volume, the Vial position and the Rack number (if required)
must be entered.
Other parameters appropriate to each acquisition channel
can be entered in the Channel X tab.
3. Click on the Start button to start the acquisition if the system
is a single-injector system or click on the Next button if the
system is a multiple injector system. In a multiple injector
system, the same parameters entered for the first injector
must be entered for the other injectors before starting the
acquisition.
Sequence
1. To create a sequence slect the FILE / NEW SEQUENCE
menu and complete the displayed creation wizard screen
(refer to the Galaxie Chromatography Data System User’s
Guide), by entering the system name that will perform the
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