Arrayit NanoPrint LM60 User manual

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User manual

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NanoPrint™ Enterprise Level LM60 and LM210
Microarray Printers
Users Manual
v.130710.1
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Table of Contents
Introduction – page 4
System Overview – page 5
Software Features – page 7
System Options and Advantages – page 8
System Specifications and Features – page 9
System Installation and Performance – page 14
Ordering Information – page 22
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Introduction
NanoPrint™ microarrayer printers are enterprise level linear drive systems that provide
universal platforms for research and diagnostic microarray manufacturing. Based on
advanced nanotechnology, Arrayit NanoPrint™ robots move with 500 nanometer resolution
and deliver sub-nanoliter sample volumes using high throughput, patented contact printing
devices. Arrayit printing devices are covered by U.S. Patent 6,101,946 and these devices
require a license for diagnostic applications. Please contact us for licensing information
regarding diagnostic microarray manufacturing.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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System Overview
Figure 1. Shown is the NanoPrint™ LM60 system with a capacity of three 384-well sample
microplates printing onto 60 standard glass slide substrates. Worktables for printing into
microplates, plate size glass and other micro-fluidic biochip formats are available.
Figure 2. Shown is the NanoPrint™ LM210 system with a capacity of three 384-well sample
microplates printing onto 210 standard glass slide substrates.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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NanoPrint™ Microarrayers are industrial-level platforms for all
microarray manufacturing applications including DNA microarrays,
protein microarrays, and other types of biomolecules. NanoPrint™
systems enable the manufacture of microarrays utilizing ArrayIt®
Professional, 946 and Stealth Style Micro Spotting Pins. The
NanoPrint™ uses superior linear drive motion control technology
and proprietary Warp1 controllers, and is compatible with all
standard microarray surfaces made by ArrayIt® and other glass
substrate slide open platform vendors. Systems are easily configured to print onto different
types of surfaces including 96 and 384-well plates, plate-sized glass, and proprietary
cartridges and cassettes by taking advantage of flexible and modular deck configurations
and an easy to use software interface. The Microarray Manager Software combines
unparalleled power and simplicity in a graphical user interface. Click here.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Software Features
A Method Creation Wizard
User & version control management
Custom calibration of the slide and microplate positions
Complete sample tracking
Support of input and output data files
Custom microarray designs
Customizable speed profiles and wash protocols
Automatic Method Validation
Runtime sample and spotting views as well as a Simulation Mode
Easy to use graphical Re-Print Wizard
The high speed, high precision linear servo control system of the NanoPrint™ produces
superior instrument performance that is essentially free of friction, noise and thermal
emission. Combined with the compact bench top design, user configurable worktable,
humidity and particulate control, a host of available options, and the flexible and
sophisticated software, NanoPrint™ systems offer the complete solution to high performance
microarray printing.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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System Options and Advantages
192 Pin Printhead (48 Pin Professional Printhead provided as standard)
Anti-vibration table
Peltier-driven microplate cooling
Microplate printing worktables
Custom worktables for proprietary printing cassettes, biochips and cartridges
Worktable conveyor
Microplate stackers, refrigerated with lid removal and barcode reader
Dehumidification
Extended service warranty
The value of the linear servo motor technology used on NanoPrint™ Microarrayers:
Nanometer scale positional accuracy
Quiet operation
No vibration
No heat generation
No motor dust
Superior Accuracy
Near maintenance-free operation
High speed
High load capacity
Zero "sagging" with loss of power
Safer and much more durable than stepper motors
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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System Specifications and Features
Software
Interface
Intuitive and Graphic Windows 2000 or Windows XP User
Interface
User Management
Password protected access levels for User, Manager and
Administrator
Data Files
Both import and export of data files compatible with all
current microarray detection instruments
Version Control
Complete tracking of all methods, version and backups
Automatic Method Validation
Automatically validates for compatible method
parameters
Sample and printing Views
Each available in real-time during operation
Method Creation Wizard
Only minimal information required to create microarray
printing protocols
Simulation Mode
Pre-test print methods in software prior to running
programs
Flexible
Microarray design, pin configuration, worktable layout,
source and destination type, offsets, speed profiles,
wash/dry parameters and pre-print locations
Hardware
Size (L x W x H, cm)
LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm)
Weight (kilograms)
LM60 (150 kg), LM210 (200 kg)
Positional resolution
500 nanometers using linear drive Warp1 control
Printing speed
48 spots per second or 192 Spots second using “192”
Series Pins and Printheads.
Destination
Microarray substrates, glass slides, microplates, custom
Printing technology
Arrayit Pro, 946 or Stealth pins and printheads
Number of pins
Configurable 1 to 48 at 4.5 mm centers or 1 to 192 at
2.25mm
Spot diameter
65 microns or larger to meet all applications
Minimum spot spacing
50 microns
Pre-printing
User definable
Wash/Dry station
Ultrasonic with 2 wash positions and a dry station
Number of microplates
Three standard 384-well, customizable
Number of substrates
60 substrate slides (LM60) or 210 substrate slides
(LM210)
Number of microplates
15 96-well microplates (LM60) or 45 96-well microplates
(LM210)
Microplate type
96-well or 384-well microplates
Microplate cooling
Cool 1-3 microplates, for protein microarray applications,
or use the refrigerated stacker for higher throughput and
hands free automation.
Environmental control
Fully enclosed, HEPA filtration and user-defined humidity
control
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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NanoPrint™ Flexible Deck
Configurations: Front left
view of the NanoPrint™
showing three microplates,
the wash/dry module and the
printhead and pins printing
onto glass substrates. A 48-
pin NanoPrint printhead
loaded with 48 946 Micro
Spotting Pins is printing
location 8 on the deck. The
modular deck design and
platen cassettes allow users to
easily switch between glass
substrates, microplates,
wafers and other types of
substrates.
Accessory Control Module
(ACM): The ACM unit provides
computer control for the
wash/dry, humidity, and
ultrasonication stations on the
deck of the NanoPrint™. The
ACM unit fits neatly beneath
the laboratory bench or under
the optional anti-vibration
table available with the
system. The ACM is extremely
quiet and operates only as
needed by the system.
Humidity is maintained in a
user-specified manner of
±1%. Wash buffers are stored
in carboys in front of the ACM.
NanoPrint™ Pin Cleaning
Module: NanoPrint™
microarrayers are each
equipped with a station that
provides pin washing, drying
and sonication of 1-48 pins.
The sonicator is automatically
filled and emptied during the
print run in a completely
automated manner. Systems
sensors prevent splashing and
overflowing for pin and deck
safety. Drying is accomplished
by vacuum using a quiet but
powerful ACM-controlled
function. The Pin Cleaning
Module is rugged, durable and
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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easy to maintain.
NanoPrint™ ACM Connectivity:
Easy connectivity between the
ACM and the robot and
accurate sensing of the
humidity inside the chamber
assures that proper humidity
levels are achieved and
maintained during the entire
duration of each print run.
Dehumidification is an option
available at an extra cost and
is highly recommended for
users who wish to operate
their systems in humid
environments. The
humidification and
dehumidification systems are
triggered by sensors that
automatically maintain the
levels set by the user.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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NanoPrint™ Humidity Sensing:
Accurate sensing of the
humidity inside the chamber
assures that proper humidity
levels are achieved and
maintained during the entire
duration of each print run.
Dehumidification is an option
available at an extra cost and
is highly recommended for
users who wish to operate
their systems in humid
environments. The
humidification and
dehumidification systems are
triggered by the RH sensor
that automatically maintain
the levels set by the user.
NanoPrint™ Microplate Peltier
Cooling: NanoPrint™ systems
offer sophisticated microplate
cooling via Peltier as an
affordable and highly
recommended option for users
wishing to minimize sample
evaporation during printing.
Microplate cooling is also
highly recommended for
protein microarray
applications to minimize
protein denaturation and
microbial growth in
recombinant protein samples.
The Peltier module fits directly
beneath the microplate for
highly efficient cooling while
maintaining a low deck profile.
NanoPrint™ Software: The
extraordinary and highly
intuitive graphical user
interface provides users with
software writing speed, power
and flexibility. Shown here is a
screen shot of the interstitial
printing software command
that automatically directs the
robot to deposit the printed
spots into a “orange packing”
configuration for dramatically
increased microarray printing
density.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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48 and 192 Pin Configurations
are now available. 192 Pin
configuration allows the user
to run from 1 to 192 pins at
2.25 mm center to center
spacing to pickup samples
from 1536 well plates.
Standard deliverable is 48 Pins
at 4.5mm centers for use with
384 well plates (96 well plates
can also be configured).
NanoPrint software and unique
Pin and Printhead design
allows the end user to setup
the pin tool any way that they
like. Multiplex array
processing systems, plates,
biochips micro fluidics, and
cartridges can all be easily
configured.
Superior Z-Axis Resolution for
Optimum Spot Morphology:
The Z-axis encoder on the
NanoPrint™ reads at 250-
nanometer resolution. Unlike
belt and stepper motor driven
Z-axis motion control, the
NanoPrint™ offers highly
precise resolution,
repeatability and computer
control over the speed and
acceleration settings to ensure
optimal printing onto any
surface using our patented
contact printing technology.
Optimal parameters are set at
the factory but can be easily
changed by the user for
printing onto many different
surfaces.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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System Installation and Performance
Figure 3. The NanoPrint™ deck is configured in a module manner, allowing different
worktables to be inserted and removed from the deck for different printing applications such
as glass substrates, microplates, and proprietary cassettes and cartridges. The screenshot
shows a worktable allowing printing into 15 microplates (96-well) for the NanoPrint™ LM60.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Figure 4. Data showing the printing consistency of eight different SMP3B pins in a single
print run using the NanoPrint LM60. The data were provided courtesy of W.G. Shreffler and
L. Bardina at the Mount Sinai School of Medicine (New York, USA).
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
16!
Figure 5. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100
cleanroom. The entire deck is accessible via two doors that open and close independently
for user convenience and safety. The top of the Accessory Control Module (ACM) is shown
beneath the robot.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Figure 6. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100
cleanroom. Microarray manufacturing parameters including the number of pins, substrates,
spots, technical replicates, printing pattern, worktable, printing offsets, speed, acceleration,
and many other technical aspects are completely controllable by the operator via an
intuitive graphical user interface (GUI). The NanoPrint™ MicroarrayManager Software
allows users to write completely customized printing routines in minutes. Worktables allow
the user to switch between glass substrate slides and microplates using different worktables
and software routines.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
18!
Figure 7. Shown is the NanoPrint™ 48-pin printhead designed to hold up to 48 microarray
printing pins of the Professional, 946 or Stealth series. The attachment bayonet allows the
printhead to be attached and removed for cleaning easily and precisely, eliminating the
need for system re-calibration.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Figure 8. Shown is the point of a Professional Series 3 (Pro3) microarray printing pin.
Samples load into the pin uptake channel by capillary action, and direct contact between the
sample layer on the pin tip and the glass printing substrate results in the formation of a
printed microarray spot. The NanoPrint™ LM60 and LM210 systems control pin movements
in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per
second. A total of 48 microarray printing pins of any tip size from the Professional, 946 or
Stealth series may be used on our NanoPrint™ systems.
Copyright 1993-2013 Arrayit Corporation. All rights reserved. 7/10/13
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Figure 9. Shown is the Worktable Workstation for the NanoPrint™ LM60 and LM210
systems. The Worktable Workstation allows fully automated conveyor-based loading and
unloading of glass slide substrate, microplate, and custom worktables from the NanoPrint™
deck. With a capacity of 60 worktables, the WorkTable Workstation allows fully automated
printing onto 1800 glass substrates, 300 96-well microplates, or 900 custom cassettes by
special purchase quoted separately from standard NanoPrint configurations.
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Arrayit NanoPrint LM60 User manual

Type
User manual
This manual is also suitable for

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