Arrayit NanoPrint LM60 User manual

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NanoPrint™ Enterprise Level LM60 and LM210

Microarray Printers

Users Manual

v.130710.1

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Table of Contents

Introduction – page 4

System Overview – page 5

Software Features – page 7

System Options and Advantages – page 8

System Specifications and Features – page 9

System Installation and Performance – page 14

Ordering Information – page 22

4!

Introduction

NanoPrint™ microarrayer printers are enterprise level linear drive systems that provide

universal platforms for research and diagnostic microarray manufacturing. Based on

advanced nanotechnology, Arrayit NanoPrint™ robots move with 500 nanometer resolution

and deliver sub-nanoliter sample volumes using high throughput, patented contact printing

devices. Arrayit printing devices are covered by U.S. Patent 6,101,946 and these devices

require a license for diagnostic applications. Please contact us for licensing information

regarding diagnostic microarray manufacturing.

5!

System Overview

Figure 1. Shown is the NanoPrint™ LM60 system with a capacity of three 384-well sample

microplates printing onto 60 standard glass slide substrates. Worktables for printing into

microplates, plate size glass and other micro-fluidic biochip formats are available.

Figure 2. Shown is the NanoPrint™ LM210 system with a capacity of three 384-well sample

microplates printing onto 210 standard glass slide substrates.

6!

NanoPrint™ Microarrayers are industrial-level platforms for all

microarray manufacturing applications including DNA microarrays,

protein microarrays, and other types of biomolecules. NanoPrint™

systems enable the manufacture of microarrays utilizing ArrayIt®

Professional, 946 and Stealth Style Micro Spotting Pins. The

NanoPrint™ uses superior linear drive motion control technology

and proprietary Warp1 controllers, and is compatible with all

standard microarray surfaces made by ArrayIt® and other glass

substrate slide open platform vendors. Systems are easily configured to print onto different

types of surfaces including 96 and 384-well plates, plate-sized glass, and proprietary

cartridges and cassettes by taking advantage of flexible and modular deck configurations

and an easy to use software interface. The Microarray Manager Software combines

unparalleled power and simplicity in a graphical user interface. Click here.

7!

Software Features

• A Method Creation Wizard

• User & version control management

• Custom calibration of the slide and microplate positions

• Complete sample tracking

• Support of input and output data files

• Custom microarray designs

• Customizable speed profiles and wash protocols

• Automatic Method Validation

• Runtime sample and spotting views as well as a Simulation Mode

• Easy to use graphical Re-Print Wizard

The high speed, high precision linear servo control system of the NanoPrint™ produces

superior instrument performance that is essentially free of friction, noise and thermal

emission. Combined with the compact bench top design, user configurable worktable,

humidity and particulate control, a host of available options, and the flexible and

sophisticated software, NanoPrint™ systems offer the complete solution to high performance

microarray printing.

8!

System Options and Advantages

• 192 Pin Printhead (48 Pin Professional Printhead provided as standard)

• Anti-vibration table

• Peltier-driven microplate cooling

• Microplate printing worktables

• Custom worktables for proprietary printing cassettes, biochips and cartridges

• Worktable conveyor

• Microplate stackers, refrigerated with lid removal and barcode reader

• Dehumidification

• Extended service warranty

The value of the linear servo motor technology used on NanoPrint™ Microarrayers:

• Nanometer scale positional accuracy

• Quiet operation

• No vibration

• No heat generation

• No motor dust

• Superior Accuracy

• Near maintenance-free operation

• High speed

• High load capacity

• Zero "sagging" with loss of power

• Safer and much more durable than stepper motors

9!

System Specifications and Features

Software

Interface

Intuitive and Graphic Windows 2000 or Windows XP User

Interface

User Management

Password protected access levels for User, Manager and

Administrator

Data Files

Both import and export of data files compatible with all

current microarray detection instruments

Version Control

Complete tracking of all methods, version and backups

Automatic Method Validation

Automatically validates for compatible method

parameters

Sample and printing Views

Each available in real-time during operation

Method Creation Wizard

Only minimal information required to create microarray

printing protocols

Simulation Mode

Pre-test print methods in software prior to running

programs

Flexible

Microarray design, pin configuration, worktable layout,

source and destination type, offsets, speed profiles,

wash/dry parameters and pre-print locations

Hardware

Size (L x W x H, cm)

LM60 (110 x 85 x 56 cm), LM210 (164 x 85 x 56 cm)

Weight (kilograms)

LM60 (150 kg), LM210 (200 kg)

Positional resolution

500 nanometers using linear drive Warp1 control

Printing speed

48 spots per second or 192 Spots second using “192”

Series Pins and Printheads.

Destination

Microarray substrates, glass slides, microplates, custom

Printing technology

Arrayit Pro, 946 or Stealth pins and printheads

Number of pins

Configurable 1 to 48 at 4.5 mm centers or 1 to 192 at

2.25mm

Spot diameter

65 microns or larger to meet all applications

Minimum spot spacing

50 microns

Pre-printing

User definable

Wash/Dry station

Ultrasonic with 2 wash positions and a dry station

Number of microplates

Three standard 384-well, customizable

Number of substrates

60 substrate slides (LM60) or 210 substrate slides

(LM210)

Number of microplates

15 96-well microplates (LM60) or 45 96-well microplates

(LM210)

Microplate type

96-well or 384-well microplates

Microplate cooling

Cool 1-3 microplates, for protein microarray applications,

or use the refrigerated stacker for higher throughput and

hands free automation.

Environmental control

Fully enclosed, HEPA filtration and user-defined humidity

control

10!

NanoPrint™ Flexible Deck

Configurations: Front left

view of the NanoPrint™

showing three microplates,

the wash/dry module and the

printhead and pins printing

onto glass substrates. A 48-

pin NanoPrint printhead

loaded with 48 946 Micro

Spotting Pins is printing

location 8 on the deck. The

modular deck design and

platen cassettes allow users to

easily switch between glass

substrates, microplates,

wafers and other types of

substrates.

Accessory Control Module

(ACM): The ACM unit provides

computer control for the

wash/dry, humidity, and

ultrasonication stations on the

deck of the NanoPrint™. The

ACM unit fits neatly beneath

the laboratory bench or under

the optional anti-vibration

table available with the

system. The ACM is extremely

quiet and operates only as

needed by the system.

Humidity is maintained in a

user-specified manner of

±1%. Wash buffers are stored

in carboys in front of the ACM.

NanoPrint™ Pin Cleaning

Module: NanoPrint™

microarrayers are each

equipped with a station that

provides pin washing, drying

and sonication of 1-48 pins.

The sonicator is automatically

filled and emptied during the

print run in a completely

automated manner. Systems

sensors prevent splashing and

overflowing for pin and deck

safety. Drying is accomplished

by vacuum using a quiet but

powerful ACM-controlled

function. The Pin Cleaning

Module is rugged, durable and

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easy to maintain.

NanoPrint™ ACM Connectivity:

Easy connectivity between the

ACM and the robot and

accurate sensing of the

humidity inside the chamber

assures that proper humidity

levels are achieved and

maintained during the entire

duration of each print run.

Dehumidification is an option

available at an extra cost and

is highly recommended for

users who wish to operate

their systems in humid

environments. The

humidification and

dehumidification systems are

triggered by sensors that

automatically maintain the

levels set by the user.

12!

NanoPrint™ Humidity Sensing:

Accurate sensing of the

humidity inside the chamber

assures that proper humidity

levels are achieved and

maintained during the entire

duration of each print run.

Dehumidification is an option

available at an extra cost and

is highly recommended for

users who wish to operate

their systems in humid

environments. The

humidification and

dehumidification systems are

triggered by the RH sensor

that automatically maintain

the levels set by the user.

NanoPrint™ Microplate Peltier

Cooling: NanoPrint™ systems

offer sophisticated microplate

cooling via Peltier as an

affordable and highly

recommended option for users

wishing to minimize sample

evaporation during printing.

Microplate cooling is also

highly recommended for

protein microarray

applications to minimize

protein denaturation and

microbial growth in

recombinant protein samples.

The Peltier module fits directly

beneath the microplate for

highly efficient cooling while

maintaining a low deck profile.

NanoPrint™ Software: The

extraordinary and highly

intuitive graphical user

interface provides users with

software writing speed, power

and flexibility. Shown here is a

screen shot of the interstitial

printing software command

that automatically directs the

robot to deposit the printed

spots into a “orange packing”

configuration for dramatically

increased microarray printing

density.

13!

48 and 192 Pin Configurations

are now available. 192 Pin

configuration allows the user

to run from 1 to 192 pins at

2.25 mm center to center

spacing to pickup samples

from 1536 well plates.

Standard deliverable is 48 Pins

at 4.5mm centers for use with

384 well plates (96 well plates

can also be configured).

NanoPrint software and unique

Pin and Printhead design

allows the end user to setup

the pin tool any way that they

like. Multiplex array

processing systems, plates,

biochips micro fluidics, and

cartridges can all be easily

configured.

Superior Z-Axis Resolution for

Optimum Spot Morphology:

The Z-axis encoder on the

NanoPrint™ reads at 250-

nanometer resolution. Unlike

belt and stepper motor driven

Z-axis motion control, the

NanoPrint™ offers highly

precise resolution,

repeatability and computer

control over the speed and

acceleration settings to ensure

optimal printing onto any

surface using our patented

contact printing technology.

Optimal parameters are set at

the factory but can be easily

changed by the user for

printing onto many different

surfaces.

14!

System Installation and Performance

Figure 3. The NanoPrint™ deck is configured in a module manner, allowing different

worktables to be inserted and removed from the deck for different printing applications such

as glass substrates, microplates, and proprietary cassettes and cartridges. The screenshot

shows a worktable allowing printing into 15 microplates (96-well) for the NanoPrint™ LM60.

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Figure 4. Data showing the printing consistency of eight different SMP3B pins in a single

print run using the NanoPrint LM60. The data were provided courtesy of W.G. Shreffler and

L. Bardina at the Mount Sinai School of Medicine (New York, USA).

16!

Figure 5. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100

cleanroom. The entire deck is accessible via two doors that open and close independently

for user convenience and safety. The top of the Accessory Control Module (ACM) is shown

beneath the robot.

17!

Figure 6. Shown is the NanoPrint™ LM210 system configured in an ArrayIt® class 100

cleanroom. Microarray manufacturing parameters including the number of pins, substrates,

spots, technical replicates, printing pattern, worktable, printing offsets, speed, acceleration,

and many other technical aspects are completely controllable by the operator via an

intuitive graphical user interface (GUI). The NanoPrint™ MicroarrayManager Software

allows users to write completely customized printing routines in minutes. Worktables allow

the user to switch between glass substrate slides and microplates using different worktables

and software routines.

18!

Figure 7. Shown is the NanoPrint™ 48-pin printhead designed to hold up to 48 microarray

printing pins of the Professional, 946 or Stealth series. The attachment bayonet allows the

printhead to be attached and removed for cleaning easily and precisely, eliminating the

need for system re-calibration.

19!

Figure 8. Shown is the point of a Professional Series 3 (Pro3) microarray printing pin.

Samples load into the pin uptake channel by capillary action, and direct contact between the

sample layer on the pin tip and the glass printing substrate results in the formation of a

printed microarray spot. The NanoPrint™ LM60 and LM210 systems control pin movements

in 500 nanometer increments for unsurpassed printing precision at speeds of 48 spots per

second. A total of 48 microarray printing pins of any tip size from the Professional, 946 or

Stealth series may be used on our NanoPrint™ systems.

20!

Figure 9. Shown is the Worktable Workstation for the NanoPrint™ LM60 and LM210

systems. The Worktable Workstation allows fully automated conveyor-based loading and

unloading of glass slide substrate, microplate, and custom worktables from the NanoPrint™

deck. With a capacity of 60 worktables, the WorkTable Workstation allows fully automated

printing onto 1800 glass substrates, 300 96-well microplates, or 900 custom cassettes by

special purchase quoted separately from standard NanoPrint configurations.

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