Thermo Fisher Scientific Owl A3-1 Gel Caster Operating instructions

  • Hello! I've reviewed the user manual for the Thermo Scientific A3-1 Wide Gel System. This document provides clear instructions on how to cast gels using the Owl's End Gate Casting System. I can answer questions about the proper placement of end gates, pouring agarose solution, using combs, and preparing the gel for electrophoresis.
  • What is the recommended temperature for the agarose solution?
    What should I do after pouring the agarose?
    What is the next step after placing combs?
Part of Thermo Fisher Scientific
Thermo Scientific A3-1 Wide Gel System
Gel Casting Using Owl's End Gate Casting System
1. Place the two end gates into the tray
with the gasket side facing out.
2. Prepare and pour warm (>60o)
agarose solution onto the UVT gel
tray(s).
3. Place combs into slots provided on
the walls of the UVT gel tray.
4. Remove the end gates, place the UVT
gel tray in the buffer chamber, add
buffer, load samples, and run the gel.
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