Thermo Fisher Scientific NorthernMax 10X User guide

Brand
Thermo Fisher Scientific
Model
NorthernMax 10X
Type
User guide
NorthernMax™ 10X Denaturing Gel Buer
Catalog Number AM8676
Pub. No. MAN0019800 Rev. A.0
WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves. Safety Data Sheets (SDSs) are available from thermofisher.com/support.
Product description
The Invitrogen™ NorthernMax™ 10X Denaturing Gel Buer (Cat. No. AM8676) is a concentrated formaldehyde-based formula for the
preparation of denaturing formaldehyde/MOPS agarose gels. The 10X gel buer is for use with the NorthernMax™ system.
Contents and storage
Contents Amount Storage
NorthernMax™ 10X Denaturing Gel Buer 250 mL 4°C
Prepare a denaturing formaldehyde/MOPS 1% gel
For 100 mL of gel:
1. Melt 1 g agarose in 90 mL nuclease-free water in a microwave or on a hot plate, with frequent agitation, until the agarose is
completely in solution. Transfer the solution to a 50–60°C water bath until equilibrated.
2. Under a ventilation hood, add 10 mL of NorthernMax™ 10X Denaturing Gel Buer to the equilibrated agarose solution and mix well
by swirling.
3. Pour the agarose solution into a gel casting tray in a ventilation hood and allow it to solidify.
The gel should be approximately 0.6 cm thick for ecient transfer.
Once gel has hardened, proceed with electrophoresis in 1X MOPS buer (e.g., prepared from NorthernMax™ 10X Running Buer;
Cat. No. AM8671)
Quality control
•Nonspecific endonuclease activity: A sample is incubated with supercoiled plasmid DNA and analyzed by agarose gel
electrophoresis.
•Exonuclease activity: A sample is incubated with labeled double-stranded DNA, followed by PAGE analysis.
•RNase activity: A sample is incubated with labeled RNA and analyzed by PAGE.
•Physico-chemical testing: pH, conductivity, density, refraction index
Limited product warranty
Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the Life Technologies' General Terms and
Conditions of Sale at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you have any questions, please
contact Life Technologies at www.thermofisher.com/support.
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, CA 92008
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all
applicable Limited Use Label Licenses.
©2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
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thermofisher.com
22 December 2020
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Thermo Fisher Scientific NorthernMax 10X User guide

Brand
Thermo Fisher Scientific
Model
NorthernMax 10X
Type
User guide
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