NeuroNexus nDrive is a versatile and reusable device designed to facilitate precise implantation and adjustment of silicon probes in neuroscience research. With its innovative features, the nDrive allows researchers to:
- Implant probes at a desired depth with micrometer-level precision, minimizing tissue damage and maximizing recording quality.
- Adjust the probe position post-implantation to optimize signal quality or target specific brain regions.
- Sterilize the entire assembly using various methods, ensuring aseptic conditions during implantation.
NeuroNexus nDrive is a versatile and reusable device designed to facilitate precise implantation and adjustment of silicon probes in neuroscience research. With its innovative features, the nDrive allows researchers to:
- Implant probes at a desired depth with micrometer-level precision, minimizing tissue damage and maximizing recording quality.
- Adjust the probe position post-implantation to optimize signal quality or target specific brain regions.
- Sterilize the entire assembly using various methods, ensuring aseptic conditions during implantation.
User Manual
Updated March 05, 2021
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
Contents
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Diagrams
Exploded View
Assembled View
Procedures
Sterilization
Pre-Implantation Procedures
Implantation Procedures
Post-Implantation Procedures
Connecting To An Omnetics Connector
Probe Position Adjustment
Mounting A New Probe For The Next Surgery
Appendix
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NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
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Cap
nDrive
Positioning Screw Probe Mount Screw
Probe Mount
Mounted Probe
Base
Exploded View
Base Screw
DIAGRAMS
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
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Assembled View
nDrive Screwdriver
Stereotaxic Adaptor
IST-Adpt
IST-nDrive
Accessories
DIAGRAMS
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
p.5
Sterilization
We highly recommend sterilization by soaking in an alcohol-based cleaning solution (10
minutes maximum). Lower the entire assembly into the cleaning solution until the Probe
and Base are submerged. Caution: high temperature sterilization may deform
parts of the nDrive. Long immersion in alcohol or water will result in parts
absorbing the uid and losing structural integrity. Sterilization via UV or EtO may
be also used as long as it does not introduce high heat. User may sterilize the entire case
without disassembling the drive if using UV or EtO sterilization.
Pre-Implantation Procedures
1. Locate the assembled nDrive.
2. Disconnect the nDrive from the packaging frame. Pay close attention to the silicon
Probe to avoid damage.
3. Make any necessary modi cations to the Base. (Shortening the feet, sanding, etc.)
4. Place the nDrive onto the Base and fasten the Positioning Screw.
5. Sterilize according to the instructions listed above.
6. Mount the nDrive assembly (with Base attached) onto a Stereotaxic Manipulator
with the Stereotaxic Adaptor. (The Stereotaxic Adaptor consists of the IST-Adpt and
IST-nDrive.)
Removing the nDrive from the Packaging Frame
PROCEDURES
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
p.6
Implantation Procedures
1. Prepare animal. Perform surgery/craniotomy. Install
bone/skull screws.
2. Adjust the position of the Probe to the desired
implant depth. Be sure to account for the thickness
of the skull and the Base. Tips: some users pre-
position the initial Probe implant depth to be some
distance above the target during the surgery, then
slowly advance the Probe over a course of days/
weeks to the target depth. This may minimize tissue
damage.
3. Install the nDrive assembly to the Stereotaxic
Adaptor and to the Stereotaxic Manipulator.
4. Manipulate the nDrive assembly using the
Stereotaxic Manipulator until the Probe tip is directly
above the target region. Lower the nDrive assembly
and implant the Probe into tissue. Be careful to
avoid blood vessels and minimize any de ection or
bulking of the silicon shank. If this happens, wait and
let the Probe slowly advance itself into the tissue.
5. Attach the ground and reference wires to the
appropriate bone screws.
6. Using the feet of the Base as anchor points, attach
the Base to the skull with dental cement.
a. Avoid excess contact with nDrive assembly, as
this may shift the Probe position set by
the Stereotaxic Manipulator.
7. Once the dental cement has fully cured, carefully
release the nDrive and the Connector from the
Stereotaxtic Adaptor by loosening the screws.
8. Determine the positioning strategy for the
Connector (i.e. cement directly on the skull or to the
Cap) and take the appropriate action.
nDrive Implantation
IST-nDrive
PROCEDURES
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
p.7
NOTES
• It may be helpful to connect the headstage during the insertion and prior to the close-up in
order to make sure the Probe is working properly.
• The niPOD can provide useful impedance monitoring pre- and post-surgery
• For additional surgical guidance, view the Buzsaki lab’s JoVE article, available in the
Support section of the NeuroNexus website.
9. If necessary, adjust Probe position by turning the Positioning Screw using the nDrive
Screwdriver.
a. Avoid excess downward & lateral force.
b. One complete turn = 282μm travel.
c. Counter-clockwise turn = downwards travel
d. Clockwise turn = upwards travel
10. Once the desired Probe position/recording quality
is reached, seal the craniotomy using appropriate
materials such as sterile Vaseline or a mixture of
paraf n and mineral oil (Fisher Scienti c 10-20g in
10mL, heated at 65oC).
11. Place the Cap or Copper Mesh in place and cement
the enclosure to expose only the Connector with
access to the Positioning Screw.
nDrive Screwdriver Attached
PROCEDURES
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
p.8
Post-Implantation Procedures
Headstage Connection
It may be easier to keep the headstage connected to the Probe Connector using rubber
bands.
Post-Implantation Probe Position Adjustment
• Monitor impediance using the niPOD. This may help you determine the condition of
the electrode-tissue interface.
• Using the nDrive Screwdriver, adjust the Probe position until signal quality is improved.
• One full rotation of the Positioning Screw corresponds to 282μm.
Turning counter-clockwise = downwards travel. Turning clockwise = upwards travel.
Mounting a New Probe
The nDrive is reusable. However, the Base, Cap, Probe Mount, and Probe are not and must
be disposed of at the end of the chronic experiment.
1. To recover the nDrive, disassemble the Cap
to expose the nDrive. Depending on cement
strength, this may require destroying the Cap.
2. Unfasten the Positioning Screw and detach the
nDrive from the Base.
3. Unfasten the Probe Mount Screw and detach the
used Probe Mount from the nDrive.
4. Locate the new Probe, mounted on the new
Probe Mount.
5. Slide the Probe Mount onto the nDrive, aligning
the holes. Be careful not to break or damage the
Probe. (It may be easier to rst secure the new
Connector to the Stereotaxic Adaptor.)
6. Fasten the Probe Mount Screw to secure the
Probe Mount.
7. The nDrive is now ready for the next surgery.
Mounting a New Probe
PROCEDURES
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
p.9
Speci cations
Screw Speci cations
• Base Screw: M1.2, 2mm length
• Probe Mount Screw: M1.2, 2mm length
• Positioning Screw: #00-90 thread, 5/16” length
Acknowledgements
NeuroNexus would like to thank Dr. Liset Menendez de la Prida (Instituto Cajal CSIC) and
Dr. Bijan Pesaran (NYU) for their feedback on the nDrive design.
APPENDIX
NeuroNexus Technologies, Inc. ©2021 | 5200 S. State Street, Suite 200, Ann Arbor, Michigan USA
Telephone: +1.734.913.8858 | Fax: +1.734.786.0069 | [email protected]
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NeuroNexus nDrive is a versatile and reusable device designed to facilitate precise implantation and adjustment of silicon probes in neuroscience research. With its innovative features, the nDrive allows researchers to:
- Implant probes at a desired depth with micrometer-level precision, minimizing tissue damage and maximizing recording quality.
- Adjust the probe position post-implantation to optimize signal quality or target specific brain regions.
- Sterilize the entire assembly using various methods, ensuring aseptic conditions during implantation.
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