B
19
Pathatrix® 5-Pooling Salmonella spp. Kit Linked to Selective Agar Plates
Background
Product overview
Description of
target
microorganism
Morethan2,400Salmonellaserotypeshavebeenreported,allofwhicharepotentially
pathogenic.Salmonellaisafrequentlyreportedcauseoffoodborneillness,occurringin
bothepidemicsandinisolatedcases.Salmonellabacteriaarethecausativeagentfor
Salmonellosis.Outbreakshavebeenassociatedwithrawmeatsandpoultry,eggs,milk
anddairyproducts,seafood,coconutsauces,saladdressings,cocoa,chocolate,spices,
frozenproducts,andvegetablessuchashotpeppers.
Audience ThePathatrix®5‐PoolingSalmonellaspp.Kitisforprofessionaluseonlyandis
intendedforusebyqualifiedusersinterestedindeterminingthepresence/absenceof
Salmonellaspp.infoodsamples.Usersmayinclude,butarenotlimitedto,food
producers,foodprocessors,foodmanufacturers,retailers,andmicrobiologytesting
laboratories.
Sampling protocol ThestandardfoodsamplesizeusedinthePathatrix®Autosystemis25goffood
dilutedwith225mLofenrichmentmedium.Werecommendthatsub‐samplesfor
poolingareprocessedimmediatelyor,ifstorageisrequired,thatthesamplesare
refrigeratedat5±3°C.Samplesshouldberewarmedto37±1°Cpriortoanalysiswith
thePathatrix®Autosystem.Theremainingenrichedsampleshouldbestoredat5±3°C
forupto32hoursuntiltheresultsofthepooledsamplehavebeendetermined.
Kit sensitivity Thesamplepreparationprocedureallowsyoutodetectasfewas1–10cfufrom
25–325gramsoffoodsample.ThelimitationofthePathatrix®5‐PoolingSalmonella
spp.Kitisintheabilityofthetargettoreproduceintheenrichmentmedium,be
capturedbythemagnet,andsubsequentlybeisolatedonselectiveagarplates.
CAUTION! ThePathatrix®kithasbeenevaluatedoncookedham,chocolate,and
choppedtomatofoodmatrices.Giventhewidevarietyofproductsandmanufacturing
procedures,werecommendthatyoucheckthatthecompositionofthematricestobe
testeddoesnotaffectthereliabilityoftheresults.
Anegativeresultdoesnotguaranteetheabsenceoftargetorganismintheoriginal
sampleandmaybeduetotheinabilityoftheorganismtoadequatelyreproduceto
requiredlevelsintheenrichmentmedium(withsubsequentoutgrowthonselective
agarplates)potentiallydueto,butnotlimitedto,competitivemicroflora,sub‐lethal
injury,ormatrixinhibition.