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CHROMELEON®
Chromatography Management System
Tutorial
and
User Manual
Version: 6.80
June 2006
© Dionex 2006
Doc.: CM_Manual_680.doc
License Agreement i
CHROMELEON Software
Individual License Agreement
This legal document is an agreement between you, the end user, and
Dionex Corporation. The CHROMELEON Chromatography Management
System is provided to you only on the condition that you agree to become
bound by the terms of this agreement, which includes the software license
and the software disclaimer warranty. If you do not agree to the terms of
this agreement, promptly return the disk package(s) and the accompanying
items (including printed materials and binders or other containers) to
Dionex for a full refund.
INDIVIDUAL CHROMELEON LICENSE
1. GRANT OF LICENSE In exchange for the payment of the LICENSE
FEE, Dionex grants the LICENSEE a non-exclusive right to use and display
the CHROMELEON software (the "SOFTWARE") provided with this LICENSE
in accordance with the terms and restrictions below. The LICENSEE is the
end-user organization and its subsidiaries (excluding sub-contractors)
billed by Dionex for this LICENSE. If the organization billed is not the end-
user, the LICENSEE is the first end-user organization.
2. OWNERSHIP OF THE SOFTWARE The LICENSEE owns the
magnetic or other physical media on which the SOFTWARE is originally or
subsequently recorded, but Dionex retains title and ownership of all
SOFTWARE recorded on such media, regardless of the form in which it is
recorded. This license is not a sale of the SOFTWARE or any copy.
3. COPY RESTRICTIONS The SOFTWARE and all accompanying written
materials are copyrighted, and may contain proprietary and confidential
information of Dionex.
The LICENSEE may make one (1) copy of the original SOFTWARE
diskettes provided with this LICENSE for backup purposes only. Any such
copy automatically becomes the property of Dionex, and the LICENSEE
must include the copyright notice and any confidentiality or proprietary
legend with any such copy. The LICENSEE may copy the SOFTWARE
from the original or backup diskettes to the computers used in accordance
with the use restrictions below.
License Agreement
ii
All other copying of the SOFTWARE and the accompanying materials is
expressly forbidden. The LICENSEE may be held legally responsible for
any copyright infringement that is caused or encouraged by the failure of
the LICENSEE to abide by the terms of this license.
4. USE RESTRICTIONS Under this LICENSE, the LICENSEE may use
the SOFTWARE on one (1) single-user computer, or one (1) terminal of a
multi-user computer. The LICENSEE must purchase a CHROMELEON Data
Processing License for each additional computer on which the
SOFTWARE provided under this LICENSE is used.
The SOFTWARE may be stored on the computer's local mass storage
device, on a network file server, or both, as long as the above use
restrictions are satisfied.
The LICENSEE may not modify, adapt, translate, reverse engineer,
decompile nor disassemble the SOFTWARE, nor create derivative works
based on it. The LICENSEE may not merge the SOFTWARE with another
program. The LICENSEE may not modify, adapt, translate, or create
derivative works based on the written materials without prior consent from
Dionex.
5. TRANSFER RESTRICTIONS The SOFTWARE is licensed only to the
LICENSEE, and may not be transferred to anyone else without the prior
written consent of Dionex. Any authorized transfer of the SOFTWARE shall
be bound by the terms and conditions of this LICENSE.
6. TERMINATION This LICENSE is effective until terminated. It will
terminate automatically without notice from Dionex if the LICENSEE fails to
comply with any of its provisions. Upon termination, the LICENSEE shall
destroy the written materials and all copies of the SOFTWARE. The
LICENSEE may terminate this LICENSE by destroying all copies of the
software and accompanying written materials.
7. UPDATE POLICY Dionex may (but is not obliged to) release updated
versions of the SOFTWARE. One copy of any such versions will be
provided to the LICENSEE at no additional charge for each registered
SYSTEM that has a valid CHROMELEON Subscription Agreement
("SUBSCRIPTION") in effect at the time of the release. Any such software
update that is provided under a SUBSCRIPTION may be used to collect
data only on the one SYSTEM to which the SUBSCRIPTION applies. All
terms of this LICENSE shall apply to any updates provided to the
LICENSEE.
License Agreement iii
8. MISCELLANEOUS This LICENSE, including the disclaimer of warranty
and limited warranty, is governed by the laws of the State of California,
excluding its choice of law rules.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
The SOFTWARE and the accompanying materials (including instructions
for use) are provided "as is" without warranty of any kind. Further, Dionex
does not warranty, guarantee, or make any representations regarding the
use, or the results of the use, of the SOFTWARE or written materials in
terms of correctness, accuracy, reliability, currentness, or otherwise. The
entire risk as to the results and performance of the SOFTWARE is
assumed by the LICENSEE.
Dionex warrants to the original LICENSEE that the disks on which the
software is recorded are free from defects in material and workmanship
under normal use and service for a period of 90 days from the date of
delivery of this individual LICENSE as evidenced by a copy of the receipt.
The entire liability of Dionex and the exclusive remedy to the LICENSEE
shall be, at the discretion of Dionex, either (a) return of the purchase price
or (b) replacement of any defective disks that are returned to Dionex with a
copy of the receipt. If failure of the disks has resulted from accident, abuse,
or misapplication, Dionex shall have no responsibility to replace the disks
or refund the purchase price. Any replacement disk shall be warranted for
the remainder of the original warranty period or thirty (30) days, whichever
is longer.
THE ABOVE ARE THE ONLY WARRANTIES OF ANY KIND AND
DIONEX HEREBY DISCLAIMS ALL OTHER WARRANTIES, EITHER
EXPRESSED OR IMPLIED, INCLUDING (BUT NOT LIMITED TO) THE
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR PURPOSE, THAT ARE MADE BY DIONEX ON THIS
PRODUCT. No oral or written information or advice given by Dionex, its
dealers, distributors, agents, or employees shall create a warranty or in any
way increase the scope of this warranty, and the LICENSEE may not rely
on such information or advice.
NEITHER DIONEX NOR ANYONE ELSE WHO HAS BEEN INVOLVED IN
THE CREATION, PRODUCTION, OR DELIVERY OF THIS SOFTWARE
SHALL BE LIABLE FOR ANY DIRECT, INDIRECT, CONSEQUENTIAL,
OR INCIDENTAL DAMAGES (INCLUDING DAMAGES FOR LOSS OF
BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS
INFORMATION, AND THE LIKE) ARISING OUT OF THE USE OF OR
INABILITY TO USE THIS SOFTWARE, EVEN IF DIONEX HAS BEEN
License Agreement
iv
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. IN THE EVENT
THAT ANY LIABILITY IS IMPOSED ON DIONEX, THE LIABILITY OF
DIONEX TO THE LICENSEE OR ANY THIRD PARTY SHALL NOT
EXCEED THE PURCHASE PRICE OF THE SOFTWARE.
Some states do not allow exclusion or limitation of the duration of the
implied warranties or exclusion or limitation of liability for incidental or
consequential damages, so that the above limitations may not apply to you.
This warranty gives you specific legal rights, and you may have other
rights, which vary from state to state.
Trademarks and Product Names v
Trademarks and Product Names
Below please find a list containing various trademarks and product names.
All trademarks (™) and registered trademarks (®) belong to Dionex
Corporation unless indicated otherwise. All other trademarks or registered
trademarks are the property of their respective owners.
A
AAA-Directâ„¢ Amino Acid Analysis System
AAA-Directâ„¢ Certified
AAA-Certifiedâ„¢
AAES™ Anion Atlas® Electrolytic Suppressor
Acclaim® columns
Acrodisk® (Gelman Sciences, Inc.)
Acurateâ„¢ Flow Splitter
Advanced Computer Interface (ACI)
Advanced Gradient Pump (AGP)
AD20 or AD25 UV/Vis Absorbance Detector
AES® Atlas Electrolytic Suppressor
Alliance® (Waters Corp.)
AminoPac® Columns
AminoTrapâ„¢ Columns
AMMS®, AMMS®-ICE, or AMMS-ICE II Anion MicroMembrane Suppressor
AnchorChipsâ„¢ (Bruker BioSciences)
Anion Atlas® Electrolytic Suppressor
Anion Self-Regenerating Suppressor®
Anotopâ„¢ (Whatman)
AppliCardâ„¢
AQAâ„¢ Mass Spectrometer (Thermo Electron Corp.)
Trademarks and Product Names
vi
ARCâ„¢ Automated Run Completion
Aroclorâ„¢ (Monsanto Corp.)
AS Autosampler
AS-HV Autosampler
AS40 or AS50 Automated Sampler
AS3500 Autosampler
ASE® 100, 200, or 300 Accelerated Solvent Extractor
ASE® Solvent-Controller
ASI-100â„¢, ASI-100Tâ„¢, ASI-100Pâ„¢, or ASI-100PTâ„¢ Autosampler
ASPECâ„¢ (Gilson, Inc.)
ASRNâ„¢ Anion Self-Regenerating Neutralizer
ASRS®, ASRS® II, or ASRS® ULTRA Anion Self-Regenerating
Suppressor
ATC Anion Trap Column
Atlas® Suppressor
Auto OnGuard™ (but OnGuard®)
AutoASE® software
autoflexâ„¢ (Bruker BioSciences)
Autoion®
AutoNeutralizationâ„¢ system or technology
AutoQâ„¢ equipment qualification
AutoRegen® System
AutoSelectâ„¢
AutoSuppression® device
AXIMA-QITâ„¢ (Shimadzu Biotech)
AXP/AXP-MS Metering Pump
B
BAKER INSTRA-ANALYZED® Acids (J. T. Baker)
Biodialyserâ„¢ (AmiKa, Inc.)
Trademarks and Product Names vii
BioLC® System
BioPlusâ„¢ Columns
BioSelectâ„¢ (The Separations Group)
BorateTrapâ„¢ Column
C
CA Carbamate Analyzer
CAES® Cation Atlas® Electrolytic Suppressor
CarboPacâ„¢ MA1, PA1, PA10, PA-100 columns
Cation Atlasâ„¢ Electrolytic Suppressor
Cation MicroMembrane Suppressor®
Cation Self-Regenerating Suppressor®
CD Builderâ„¢ (AppletWare, Inc.)
CD20, CD25, CD25A Conductivity Detector
Chemraz® (Greene, Tweed & Co.)
ChromatoCrittersâ„¢
CH-2 Column Heater
CHX650 Column Temperature Controller
CHROMELEON® Chromatography Management Software
CHROMELEON® Purification Suite™
CMDâ„¢ Carbohydrate Membrane Desalter
CMMS® Cation MicroMembrane Suppressor
CMMS® II Cation MicroMembrane Suppressor®
Continuously Regenerating Anion Trap Column (CR-ATC)
Continuously Regenerating Cation Trap Column (CR-CTC)
Cryptand columns
CSRNâ„¢ Cation Self-Regenerating Neutralizer
CSRS®, CSRS® II, CSRS® ULTRA Cation Self-Regenerating Suppressor
Trademarks and Product Names
viii
D
DataDetectiveâ„¢ (AppletWare Inc.)
dBASE® (Borland International, Inc.)
Dequest® (Monsanto Corporation)
Dionex®
DNAPac® column (for NucleoPac)
DNAPhorâ„¢ SB1.5 kB Sieving Buffer Kit (for NucleoPhor)
Dowex® (Dow Chemical Company)
DX-80 Ion Analyzer
DX-120 Ion Chromatograph
DX-320 Ion Chromatograph
DX-500 or DX-600 Ion Chromatography System or HPLC System
DX-800 Process Analyzer
DX-LANâ„¢ Instrument Interface
E
ED40, ED50, or ED50A Electrochemical Detector
EG40 or EG50 Eluent Generator
Electrochrom
EluGen®
EO1 Eluent/Solvent Organizer
esquire2000â„¢(Bruker BioSciences)
esquire3000 (Bruker BioSciences)
esquireHCT (Bruker BioSciences)
F
FAMOSâ„¢ Fully Automated Micro Autosampler
FastLocâ„¢ (Thermo Electron Corp.)
Finnigan AQAâ„¢, Finnigan MSQâ„¢
Trademarks and Product Names ix
Flarefit®
FluoBoost Micro Fluorescence Flow Cell
Foxy® (Isco, Inc.)
FPLC® (Pharmacia LKB)
Freon® (E.I. du Pont de Nemours & Co.)
Fusica columns
G
GM-2, GM-3, or GM-4 Eluent Gradient Mixers
GP40 or GP50 Gradient Pump
GS50 Gradient Pump
H
HPICE® (mostly replaced by IonPac® ICE)
Hydromatrixâ„¢ (Varian Associates, Inc.)
I
ICAT® (University of Washington, Seattle, USA)
ICE (ion-exclusion columns, e.g., ICE-AS6)
ICS-90, ICS-1000, ICS-1500, ICS-2000, or ICS-2500 Ion Chromatography
System
ICS-3000 Conductivity Detector
ICS-3000 Detector/Chromatography Module
ICS-3000 Dual Pump
ICS-3000 Electrochemical Detector
ICS-3000 Eluent Generator
ICS-3000 Single Pump
InkJet® (Hewlett-Packard)
IonPac® columns
Trademarks and Product Names
x
IonPhorâ„¢ Electrolyte Buffers
IonSep® reagents
IP20, IP25, or IS25 Isocratic Pump
Irganox® (Ciba)
J
Just Add Water
K
Kalrez® (E.I. du Pont de Nemours & Co.)
KEL-F® (3M Corporation)
L
LANtastic® (Artisoft, Inc.)
LaserJet® (Hewlett-Packard Corporation)
LC5 Injection Module
LC10 Chromatography Organizer
LC20 Chromatography Enclosure
LC25 or LC30 Chromatography Oven
M
Mascot® (Matrix Science Ltd.)
MassLynxâ„¢ (Micromass)
µ-Guard™ columns
µ-Dumper
µ-Fluidics
MetPacâ„¢ Reagents
MFC-1 (Metal-Free Column)
MICRO® (International Products Corp.)
Trademarks and Product Names xi
Microcolumn
Micro Precolumn
MicroBeadâ„¢ resin
Microguard columns
MicroInjection valve
MicroMembraneâ„¢ Suppressor
Millennium® (Waters Corp.)
Milli-Q® (Millipore)
MMSâ„¢ MicroMembraneâ„¢ Suppressor
Mono Q® (Pharmacia LKB)
MonoDiskâ„¢
Monolithic capillary column
MonoStandard®
M Pathâ„¢ (Thermo Electron Corp.)
MPIC® (Mobile Phase IC)
MRAâ„¢ Active Flow Splitter (Rheodyne)
MS-DOS® (Microsoft Corporation)
MSQâ„¢ Mass Spectrometer (Thermo Electron Corp.)
N
Nano column
Nano LC
Nano precolumn
Nanospray
N-EVAP® (Organomation Associates, Inc.)
NovaPak® (Waters Corp.)
NucleoPac (now DNAPac®)
NucleoPhor (now DNAPhorâ„¢)
Trademarks and Product Names
xii
O
OligoStandardsâ„¢
OmniFLEXâ„¢ (Bruker BioSciences)
OmniPac® Columns
OnGuard®, OnGuard® II Sample Prep Station (but Auto OnGuard™)
Optimaâ„¢ (Fisher Scientific)
ORBOâ„¢ (Supelco, Inc.)
P
P680 HPLC Pump
PaintJet® (Hewlett-Packard)
PC10 Postcolumn Pneumatic Controller
PC10 Reagent Delivery Module
PD40 Diode Array Detector
PDA-100 Photodiode Array Detector
PeakNet® Chromatography Workstation
Pentium® (Intel)
PepMapâ„¢
Pico-Buffer®
PicoViewâ„¢ (New Objective, Inc.)
PolyVialâ„¢
PowerPoint® (Microsoft)
Probotâ„¢ Microfraction Collector
Process 450 (data system for the 8200 series)
ProPac® Columns
ProteinChips® (Ciphergen)
Purification Suite™ (better: Chromeleon® Purification Suite™)
PWAâ„¢ Purification Workflow Automation
Trademarks and Product Names xiii
Q
QSTAR® (Applied Biosystems)
QTRAP® (Applied Biosystems)
R
Reacti-Blockâ„¢ (Pierce Chemical Company)
Reacti-Thermâ„¢ (Pierce Chemical Company)
Reagent-Freeâ„¢
RF 2000 Fluorescence Detector
RFICâ„¢
S
SC-CSRS®
SELDI ProteinChips® (Ciphergen)
Self-Contained Cation Self-Regenerating Suppressor
SelectaPoreâ„¢ Columns (The Separations Group)
Self-Regenerating Suppressor®
SEQUEST® (University of Washington, Seattle, USA)
Series 600 SFC and SFC/GC Systems
SFE-723 Supercritical Fluid Extractor
SFMâ„¢ Sample and Fraction Manager
SmartFlowâ„¢
SP10 AutoNeutralizationâ„¢ System
SpeedVacâ„¢ (Savant Corp.)
SRC SRS Controller)
SRNâ„¢ Self-Regenerating Neutralizer
SRS® Self-Regenerating Suppressor
Summit® HPLC System
SUPELCOSILâ„¢, Supelguardâ„¢ (Supelco, Inc.)
Trademarks and Product Names
xiv
Superba columns
Superose® (Pharmacia)
Supor® (Pall Corporation)
SupraPur® (EM Industries, Inc.)
Switchosâ„¢ Microcolumn Switching Device
T
TAC-1 (Trace Anion Concentrator)
TCC-1 (Trace Cation Concentrator)
TCC-100 (Thermostatted Column Compartment)
Teflon®, Tefzel® (E.I. du Pont de Nemours & Co.)
ThermoFlareâ„¢
Thermos Column Oven
Triton® X-100 (Rohm & Haas)
TurboIonTrap® (Applied Biosystems)
TurboVap® (Zymark Corporation)
Tween® 20 (Atlas Chemical Co.)
U
UCI-100 or UCI-50 Universal Chromatography Interface
UI20 Universal Interface
ultraflex (Bruker BioSciences)
UltiChromâ„¢ software
UltiMateâ„¢ 3000 LC System
UltiMateâ„¢ Dual Nano and Capillary LC System
UltiMateâ„¢ Nano and Capillary LC System
UltiMateâ„¢ Plus
UltiMateâ„¢ x2
Ultrex® (J.T. Baker)
Trademarks and Product Names xv
UV-Booster
UVD 170S/170U HPLC UV/Vis Detectors
UVD 340S/340U HPLC Photodiode Array Detectors
U-Z Viewâ„¢ Capillary Flow Cell
V
Vespel® (E.I. du Pont de Nemours & Co.)
VHPâ„¢ (The Separations Group, Inc.)
Virtual Columnâ„¢ separation simulation tool
Vortex-Genie® (Scientific Industries, Inc)
Voyagerâ„¢ (Applied Biosystems)
Vydac® columns (The Separations Group, Inc.)
W
Windows® 2000/XP (Microsoft Corporation)
Windows NT® (Microsoft Corporation)
Wonderware InTouch® (Wonderware Corporation)
Z
Xcalibur® (Thermo Electron Corp.)
Zitex® (Norton Chemplast)
Zorbax® (E.I. du Pont de Nemours & Co.)
Trademarks and Product Names
xvi
Abbreviations xvii
Abbreviations
Below please find a list of the most frequently used abbreviations in
Chromeleon, the online Help, and the manual:
APS Automated Purification System
¾
ARC Automated Run Completion
¾
AIA Analytical Instrument Association
BCD ¾Binary-Coded Decimal Code
BOOTP Bootstrap Protocol (Internet protocol)
CAN Controller Area Network
CD Compact Disk
CDS Chromatography Data System
CE Capillary Electrophoresis
CFR Code of Federal Regulations (often synonymous with 21 CFR
Part 11)
CM Chromeleon® Chromatography Management System
CMB ¾Backup container/file (file extension)
CMS Chromatography Management System
CS Cluster Server
DAC Digital Analog Converter
DHCP Dynamic Host Communication Protocol
DNS Domain Name Server
DX Dionex
EMF Early Maintenance Feedback
GC Gas Chromatography
¾
GLP Good Laboratory Practice
GMP Good Manufacturing Practice
GPIB General Purpose Interface Bus
HPLC High Performance Liquid Chromatography
Abbreviations
xviii
IC Ion Chromatography
IPC Chromeleon Instruments Server or Instruments PC
¾
IQ Installation Qualification
¾
ISTD Internal Standard
LAN Local Area Network
LIB UV Spectra Library (file extension)
LIC Chromeleon ¾License Server
¾
LIMS Laboratory Information Management System
LOD ¾Limit of Detection
MAC Media Access Control address (unique address of each network
interface card)
MRA Mass Rate Attenuation (Flow Splitter)
MS ¾Mass Spectrometer
MSV Motorized switching valve
NFP ¾Network Failure Protection
¾
ODBC Microsoft Open Database Connectivity
¾
OQ Operational Qualification
PAN ¾Control Panel for chromatographic system control (file
extension)
¾
PDA Photodiode Array Detector
¾
PGM Control program for chromatographic system control (file
extension)
PN PeakNet® Chromatography Management System
¾
PPA Peak Purity Analysis
¾
PPI Peak Purity Index
¾
PQ Performance Qualification
¾
PWA Purification Workflow Automation
¾
QNT Quantification method for signal interpretation and evaluation (file
extension)
QRF ¾Query file (file extension)
/
