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CHROMELEON®
Chromatography Management System
Tutorial
and
User Manual
Version: 6.60
March 2004
© Dionex 2004
Doc.: CM_Manual_660.doc
License Agreement i
CHROMELEON Software
Individual License Agreement
This legal document is an agreement between you, the end user, and
Dionex Corporation. The CHROMELEON Chromatography Management
System is provided to you only on the condition that you agree to become
bound by the terms of this agreement, which includes the software license
and the software disclaimer warranty. If you do not agree to the terms of
this agreement, promptly return the disk package(s) and the accompanying
items (including printed materials and binders or other containers) to
Dionex for a full refund.
INDIVIDUAL CHROMELEON LICENSE
1. GRANT OF LICENSE In exchange for the payment of the LICENSE
FEE, Dionex grants the LICENSEE a non-exclusive right to use and display
the CHROMELEON software (the "SOFTWARE") provided with this LICENSE
in accordance with the terms and restrictions below. The LICENSEE is the
end-user organization and its subsidiaries (excluding sub-contractors)
billed by Dionex for this LICENSE. If the organization billed is not the end-
user, the LICENSEE is the first end-user organization.
2. OWNERSHIP OF THE SOFTWARE The LICENSEE owns the
magnetic or other physical media on which the SOFTWARE is originally or
subsequently recorded, but Dionex retains title and ownership of all
SOFTWARE recorded on such media, regardless of the form in which it is
recorded. This license is not a sale of the SOFTWARE or any copy.
3. COPY RESTRICTIONS The SOFTWARE and all accompanying written
materials are copyrighted, and may contain proprietary and confidential
information of Dionex.
The LICENSEE may make one (1) copy of the original SOFTWARE
diskettes provided with this LICENSE for backup purposes only. Any such
copy automatically becomes the property of Dionex, and the LICENSEE
must include the copyright notice and any confidentiality or proprietary
legend with any such copy. The LICENSEE may copy the SOFTWARE
from the original or backup diskettes to the computers used in accordance
with the use restrictions below.
License Agreement
ii
All other copying of the SOFTWARE and the accompanying materials is
expressly forbidden. The LICENSEE may be held legally responsible for
any copyright infringement that is caused or encouraged by the failure of
the LICENSEE to abide by the terms of this license.
4. USE RESTRICTIONS Under this LICENSE, the LICENSEE may use
the SOFTWARE on one (1) single-user computer, or one (1) terminal of a
multi-user computer. The LICENSEE must purchase a CHROMELEON Data
Processing License for each additional computer on which the
SOFTWARE provided under this LICENSE is used.
The SOFTWARE may be stored on the computer's local mass storage
device, on a network file server, or both, as long as the above use
restrictions are satisfied.
The LICENSEE may not modify, adapt, translate, reverse engineer,
decompile nor disassemble the SOFTWARE, nor create derivative works
based on it. The LICENSEE may not merge the SOFTWARE with another
program. The LICENSEE may not modify, adapt, translate, or create
derivative works based on the written materials without prior consent from
Dionex.
5. TRANSFER RESTRICTIONS The SOFTWARE is licensed only to the
LICENSEE, and may not be transferred to anyone else without the prior
written consent of Dionex. Any authorized transfer of the SOFTWARE shall
be bound by the terms and conditions of this LICENSE.
6. TERMINATION This LICENSE is effective until terminated. It will
terminate automatically without notice from Dionex if the LICENSEE fails to
comply with any of its provisions. Upon termination, the LICENSEE shall
destroy the written materials and all copies of the SOFTWARE. The
LICENSEE may terminate this LICENSE by destroying all copies of the
software and accompanying written materials.
7. UPDATE POLICY Dionex may (but is not obliged to) release updated
versions of the SOFTWARE. One copy of any such versions will be
provided to the LICENSEE at no additional charge for each registered
SYSTEM that has a valid CHROMELEON Subscription Agreement
("SUBSCRIPTION") in effect at the time of the release. Any such software
update that is provided under a SUBSCRIPTION may be used to collect
data only on the one SYSTEM to which the SUBSCRIPTION applies. All
terms of this LICENSE shall apply to any updates provided to the
LICENSEE.
License Agreement iii
8. MISCELLANEOUS This LICENSE, including the disclaimer of warranty
and limited warranty, is governed by the laws of the State of California,
excluding its choice of law rules.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
The SOFTWARE and the accompanying materials (including instructions
for use) are provided "as is" without warranty of any kind. Further, Dionex
does not warranty, guarantee, or make any representations regarding the
use, or the results of the use, of the SOFTWARE or written materials in
terms of correctness, accuracy, reliability, currentness, or otherwise. The
entire risk as to the results and performance of the SOFTWARE is
assumed by the LICENSEE.
Dionex warrants to the original LICENSEE that the disks on which the
software is recorded are free from defects in material and workmanship
under normal use and service for a period of 90 days from the date of
delivery of this individual LICENSE as evidenced by a copy of the receipt.
The entire liability of Dionex and the exclusive remedy to the LICENSEE
shall be, at the discretion of Dionex, either (a) return of the purchase price
or (b) replacement of any defective disks that are returned to Dionex with a
copy of the receipt. If failure of the disks has resulted from accident, abuse,
or misapplication, Dionex shall have no responsibility to replace the disks
or refund the purchase price. Any replacement disk shall be warranted for
the remainder of the original warranty period or thirty (30) days, whichever
is longer.
THE ABOVE ARE THE ONLY WARRANTIES OF ANY KIND AND
DIONEX HEREBY DISCLAIMS ALL OTHER WARRANTIES, EITHER
EXPRESSED OR IMPLIED, INCLUDING (BUT NOT LIMITED TO) THE
IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
PARTICULAR PURPOSE, THAT ARE MADE BY DIONEX ON THIS
PRODUCT. No oral or written information or advice given by Dionex, its
dealers, distributors, agents, or employees shall create a warranty or in any
way increase the scope of this warranty, and the LICENSEE may not rely
on such information or advice.
NEITHER DIONEX NOR ANYONE ELSE WHO HAS BEEN INVOLVED IN
THE CREATION, PRODUCTION, OR DELIVERY OF THIS SOFTWARE
SHALL BE LIABLE FOR ANY DIRECT, INDIRECT, CONSEQUENTIAL,
OR INCIDENTAL DAMAGES (INCLUDING DAMAGES FOR LOSS OF
BUSINESS PROFITS, BUSINESS INTERRUPTION, LOSS OF BUSINESS
INFORMATION, AND THE LIKE) ARISING OUT OF THE USE OF OR
INABILITY TO USE THIS SOFTWARE, EVEN IF DIONEX HAS BEEN
License Agreement
iv
ADVISED OF THE POSSIBILITY OF SUCH DAMAGES. IN THE EVENT
THAT ANY LIABILITY IS IMPOSED ON DIONEX, THE LIABILITY OF
DIONEX TO THE LICENSEE OR ANY THIRD PARTY SHALL NOT
EXCEED THE PURCHASE PRICE OF THE SOFTWARE.
Some states do not allow exclusion or limitation of the duration of the
implied warranties or exclusion or limitation of liability for incidental or
consequential damages, so that the above limitations may not apply to you.
This warranty gives you specific legal rights, and you may have other
rights, which vary from state to state.
Trademarks and Product Names v
Trademarks and Product Names
Below please find a list containing various trademarks and product names.
All trademarks (™) and registered trademarks (®) belong to Dionex
Corporation unless indicated otherwise. All other trademarks or registered
trademarks are the property of their respective owners.
A
AAA-Directâ„¢ Amino Acid Analysis System
AAA-Certifiedâ„¢
AAESâ„¢
Acclaim® columns
Acrodisk® (Gelman Sciences, Inc.)
Acurateâ„¢ Flow Splitter
Advanced Computer Interface (ACI)
Advanced Gradient Pump (AGP)
AD20 UV/Vis Absorbance Detector
AES® Atlas Electrolytic Suppressor
Alliance® (Waters Corp.)
AminoPac® Columns
AminoTrapâ„¢ Columns
AMMS®-ICE, AMMS-ICE II Anion MicroMembrane Suppressor
AnchorChipsâ„¢ (Bruker BioSciences)
Anion Atlas® Electrolytic Suppressor
Anion Self-Regenerating Suppressor®
Anotopâ„¢ (Whatman)
AppliCardâ„¢
AQAâ„¢ Mass Spectrometer (Thermo Electron Corp.)
Trademarks and Product Names
vi
Aroclorâ„¢ (Monsanto Corp.)
AS40, AS50 Automated Sampler
AS3500 Autosampler
ASE® 100, 200, or 300 Accelerated Solvent Extractor or Solvent Controller
ASI-100â„¢ or ASI-100Tâ„¢ Automated Sample Injector
ASI-100Pâ„¢ or ASI-100PTâ„¢ Automated Sample Injector
ASRNâ„¢ Anion Self-Regenerating Neutralizer
ASRS®, ASRS® II, or ASRS® ULTRA Anion Self-Regenerating Suppressor
Atlas® Suppressor
Auto OnGuard™ (but OnGuard®)
AutoASE® software
autoflexâ„¢ (Bruker BioSciences)
Autoion®
AutoNeutralizationâ„¢ system or technology
AutoQâ„¢
AutoRegen® System
AutoSelectâ„¢
AutoSuppression® device
AXIMA-QITâ„¢ (Shimadzu Biotech)
B
BAKER INSTRA-ANALYZED® Acids (J. T. Baker)
Biodialyserâ„¢ (AmiKa, Inc.)
BioLC® System
BioPlusâ„¢ Columns
BioSelectâ„¢ (The Separations Group)
BorateTrapâ„¢ Column
Trademarks and Product Names vii
C
CA Carbamate Analyzer
CAES® Cation Atlas® Electrolytic Suppressor
CarboPacâ„¢ MA1, PA1, PA10, PA-100 columns
Cation Atlasâ„¢ Electrolytic Suppressor
Cation MicroMembrane Suppressor®
Cation Self-Regenerating Suppressor®
CD Builderâ„¢ (AppletWare Inc.)
Chemraz® (Greene, Tweed & Co.)
ChromatoCrittersâ„¢
CH-2 Column Heater
CHX650 Column Temperature Controller
CHROMELEON® Chromatography Management Software
CMDâ„¢ Carbohydrate Membrane Desalter
CMMS® Cation MicroMembrane Suppressor
Continuously Regenerating Anion Trap Column (CR-ATC)
Continuously Regenerating Cation Trap Column (CR-CTC)
CSRNâ„¢ Cation Self-Regenerating Neutralizer
CSRS®, CSRS® II, CSRS® ULTRA Cation Self-Regenerating Suppressor
CD20 Conductivity Detector
CMMS® II Cation MicroMembrane Suppressor®
Cryptand columns
D
DataDetectiveâ„¢ (AppletWare Inc.)
dBASE® (Borland International, Inc.)
Dequest® (Monsanto Corporation)
Dionex®
Trademarks and Product Names
viii
DNAPac® column (for NucleoPac)
DNAPhorâ„¢ SB1.5 kB Sieving Buffer Kit (for NucleoPhor)
Dowex® (Dow Chemical Company)
DX-80 Ion Analyzer
DX-120 Ion Chromatograph
DX-320 Ion Chromatograph
DX-500 or DX-600 Ion Chromatography System or HPLC System
DX-800 Process Analyzer
DX-LANâ„¢ Instrument Interface
E
ED40, ED50 Electrochemical Detector
EG40, EG50 Eluent Generator
Electrochrom
EluGen®
EO1 Eluent/Solvent Organizer
F
FAMOSâ„¢ Fully Automated Micro Autosampler
FastLocâ„¢ (Thermo Electron Corp.)
Finnigan AQAâ„¢, Finnigan MSQâ„¢
Flarefit®
FluoBoost Micro Fluorescence Flow Cell
Foxy® (Isco, Inc.)
FPLC® (Pharmacia LKB)
Freon® (E.I. du Pont de Nemours & Co.)
Fusica columns
Trademarks and Product Names ix
G
GM-2, GM-3, or GM-4 Eluent Gradient Mixers
GP40, GP50 Gradient Pump
GS50 Gradient Pump
H
HPICE® (mostly replaced by IonPac® ICE)
Hydromatrixâ„¢ (Varian Associates, Inc.)
I
ICE (ion-exclusion columns, e.g., ICE-AS6)
ICS-90, ICS-1000, ICS-1500, ICS-2000, ICS-2500
InkJet® (Hewlett-Packard)
IonPac® columns
IonPhorâ„¢ Electrolyte Buffers
IonSep® reagents
IP20 Isocratic Pump
Irganox® (Ciba)
J
Just Add Water
K
Kalrez® (E.I. du Pont de Nemours & Co.)
KEL-F® (3M Corporation)
Trademarks and Product Names
x
L
LANtastic® (Artisoft, Inc.)
LaserJet® (Hewlett-Packard Corporation)
LC5 Injection Module
LC10 Chromatography Organizer
LC20 Chromatography Enclosure
LC25, LC30 Chromatography Oven
M
Mascot® (Matrix Science Ltd.)
MassLynxâ„¢ (Micromass)
µ-Guard™ columns
µ-Dumper
µ-Fluidics
MetPacâ„¢ Reagents
MFC-1 (Metal-Free Column)
MICRO® (International Products Corp.)
Micro Precolumn
MicroBeadâ„¢ resin
MicroInjection valve
MicroMembraneâ„¢ Suppressor
Millennium® (Waters Corp.)
Milli-Q® (Millipore)
MMSâ„¢ MicroMembraneâ„¢ Suppressor
Monolithic capillary column
Mono Q® (Pharmacia LKB)
Trademarks and Product Names xi
MonoStandard®
M Pathâ„¢ (Thermo Electron Corp.)
MPIC® (Mobile Phase IC)
MS-DOS® (Microsoft Corporation)
MSQâ„¢ Mass Spectrometer (Thermo Electron Corp.)
N
Nano precolumn
N-EVAP® (Organomation Associates, Inc.)
NovaPak® (Waters Corp.)
NucleoPac (now DNAPac®)
NucleoPhor (now DNAPhorâ„¢)
O
OligoStandardsâ„¢
OmniFLEXâ„¢ (Bruker BioSciences)
OmniPac® Columns
OnGuard®, OnGuard® II Sample Prep Station (but Auto OnGuard™)
Optimaâ„¢ (Fisher Scientific)
ORBOâ„¢ (Supelco, Inc.)
P
P680 HPLC Pump
PaintJet® (Hewlett-Packard)
PC10 Postcolumn Pneumatic Controller
PC10 Reagent Delivery Module
PD40 Diode Array Detector
PDA-100 Photodiode Array Detector
Trademarks and Product Names
xii
PeakNet® Chromatography Workstation
Pentium® (Intel)
PepMapâ„¢
Pico-Buffer®
PicoViewâ„¢ (New Objective, Inc.)
PolyVialâ„¢
PowerPoint® (Microsoft)
Probotâ„¢ Microfraction Collector
Process 450 (Datensystem für 8200er-Serie)
ProPac® Columns
ProteinChips® (Ciphergen)
Q
QSTAR® (Applied Biosystems)
R
Reacti-Thermâ„¢ (Pierce Chemical Company)
Reagent-Freeâ„¢
S
SC-CSRS®
SELDI ProteinChips® (Ciphergen)
Self-Contained Cation Self-Regenerating Suppressor
SelectaPoreâ„¢ Columns (The Separations Group)
Self-Regenerating Suppressor®
SEQUEST® (University of Washington, Seattle, USA)
Series 600 SFC and SFC/GC Systems
SFE-723 Supercritical Fluid Extractor
SmartFlowâ„¢
Trademarks and Product Names xiii
SP10 AutoNeutralizationâ„¢ System
SpeedVacâ„¢ (Savant Corp.)
SRC SRS Controller)
SRNâ„¢ Self-Regenerating Neutralizer
SRS® Self-Regenerating Suppressor
Summit® HPLC System
SUPELCOSILâ„¢, Supelguardâ„¢ (Supelco, Inc.)
Superba columns
Superose® (Pharmacia)
Supor® (Pall Corporation)
SupraPur® (EM Industries, Inc.)
Switchosâ„¢ Microcolumn Switching Device
T
TAC-1 (Trace Anion Concentrator)
TCC-1 (Trace Cation Concentrator)
TCC-100 (Thermostatted Column Compartment)
Teflon®, Tefzel® (E.I. du Pont de Nemours & Co.)
ThermoFlareâ„¢
Thermos Column Oven
Triton® X-100 (Rohm & Haas)
TurboVap® (Zymark Corporation)
Tween® 20 (Atlas Chemical Co.)
U
UCI-100 or UCI-50 Universal Chromatography Interface
UI20 Universal Interface
ultraflex (Bruker BioSciences)
Trademarks and Product Names
xiv
UltiChromâ„¢ software
UltiMateâ„¢
Ultrex® (J.T. Baker)
UV-Booster
UVD 170S/170U UV/Vis Detector
UVD 340S/340U Photodiode Array Detector
U-Z Viewâ„¢ Capillary Flow Cell
V
Vespel® (E.I. du Pont de Nemours & Co.)
VHPâ„¢ (The Separations Group, Inc.)
Vortex-Genie® (Scientific Industries, Inc)
Voyagerâ„¢ (Applied Biosystems)
Vydac® columns (The Separations Group, Inc.)
W
Windows® 2000/XP (Microsoft Corporation)
Windows NT® (Microsoft Corporation)
Wonderware InTouch® (Wonderware Corporation)
Z
Zitex® (Norton Chemplast)
Zorbax® (E.I. du Pont de Nemours & Co.)
Abbreviations xv
Abbreviations
Below please find a list of the most frequently used abbreviations in
Chromeleon, the online Help, and the manual:
¾AIA Analytical Instrument Association
BCD ¾Binary-Coded Decimal Code
BOOTP Bootstrap Protocol (Internet protocol)
CAN Controller Area Network
CD Compact Disk
CDS Chromatography Data System
CE Capillary Electrophoresis
CFR Code of Federal Regulations
(often synonymous with 21 CFR Part 11)
CM Chromeleon® Chromatography Management System
CMB ¾Backup container/file (file extension)
CMS Chromatography Management System
CS Cluster Server
DHCP Dynamic Host Communication Protocol
DNS Domain Name Server
DX Dionex
GC Gas Chromatography
¾GLP Good Laboratory Practice
GMP Good Manufacturing Practice
GPIB General Purpose Interface Bus
HPLC High Performance Liquid Chromatography
IC Ion Chromatography
IPC Chromeleon Instruments Server or Instruments PC
¾IQ Installation Qualification
Abbreviations
xvi
ISTD Internal Standard
LAN Local Area Network
LIB UV Spectra Library (file extension)
LIC Chromeleon ¾License Server
¾LIMS Laboratory Information Management System
LOD Limit of Detection
MAC Media Access Control address
(unique address of each network interface card)
MS ¾Mass Spectrometer
NFP ¾Network Failure Protection
¾ODBC Microsoft Open Database Connectivity
¾OQ Operational Qualification
PAN ¾Control Panel for chromatographic system control
(file extension)
¾PDA Photodiode Array Detector
¾PGM Control program for chromatographic system control
(file extension)
PN PeakNet® Chromatography Management System
¾PPA Peak Purity Analysis
¾PPI Peak Purity Index
¾PQ Performance Qualification
¾QNT Quantification method for signal interpretation and evaluation
(file extension)
QRF ¾Query file (file extension)
RDF ¾Report Definition File; includes the layout definition
for the report and export (file extension)
¾RPC Remote Procedure Call
RSD Relative Standard Deviation
¾SDK Software Development Kit for Chromeleon
Abbreviations xvii
SEQ ¾Sequence; that is, the sequence of samples plus the
corresponding files (file extension)
¾SIM Selected Ion Monitoring (MS channel for specific masses)
¾SOR Signed Off Results (File) = signed sequence
¾SQL Structured Query Language (computer language)
SST ¾System Suitability Test
TCP/IP Transmission Control Protocol/Internet Protocol
(class of Internet protocols)
¾TIC Total Ion Current
(MS channel for the entire mass range or a part thereof)
¾TTL Transistor-Transistor Logic
UDP User Datagram Protocol (IP communication protocol)
UDC ¾User-Defined Column
USB Universal Serial Bus
VCD ¾Virtual Channel Driver (driver for virtual devices)
WSP Workspace; that is, the definition of a screen layout
(file extension)
For a list of the ¾Institutes and Institutions for Industry Standards, refer to
the Glossary.
Abbreviations
xviii
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