Thermo Fisher Scientific Agilent 1100 Series User guide

Brand: Thermo Fisher Scientific

Model: Agilent 1100 Series

Type: User guide

Thermo

Agilent 1100 Series

Getting Connected Guide for LC Devices

XCALI-97190 Revision J December 2013

Foundation and MSQ are trademarks, and LCQ, LCQ Fleet, LTQ, LXQ, Surveyor, Thermo Scientific, and

Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.

The following are registered trademarks in the United States and other countries:

Adobe and Reader are registered trademarks of Adobe Systems Incorporated. Agilent is a registered trademark

of Agilent Technologies Inc. Dell is a registered trademark of Dell Inc. Hewlett Packard and JetDirect are

registered trademarks of Hewlett-Packard Development Company. Microsoft and Windows are registered

trademarks of Microsoft Corporation.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Revision A, Feb 2007; Revision B, July 2007; Revision C, Jan 2009; Revision D, Feb 2011;

Revision E, Dec 2011; Revision F, May 2012; Revision G, July 2012; Revision H, Dec 2012; Revision J,

Dec 2013

Software version: Thermo Foundation (see page vii), Thermo LC Devices 2.2.1 or later

For Research Use Only. Not for use in diagnostic procedures.

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices iii

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

System Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Supported Firmware Versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix

Cautions and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . x

Chapter 1 Checking the Agilent Firmware Versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Chapter 2 Connecting the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

Ethernet Communication Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Installing a Network Card in an Agilent Module. . . . . . . . . . . . . . . . . . . . . . . . . 4

Connecting the Ethernet Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Installing the External Contact Interface Board . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Connecting the Contact Closure Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Chapter 3 Specifying the TCP/IP Settings and the BCD Board Location. . . . . . . . . . . . . . . .11

Assigning Contact Closure Control to the Autosampler . . . . . . . . . . . . . . . . . . 11

Specifying the TCP/IP Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Chapter 4 Triggering Data Acquisition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

Chapter 5 Turning Off the Solvent Tracking Feature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Chapter 6 Upgrading an HP 1100 Series LC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Contents

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices v

Preface

This guide describes how to connect an Agilent™ 1100 Series liquid chromatograph (LC)

system to the Thermo Scientific™ mass spectrometer (MS) and the data system computer, and

how to establish communication between the LC system and a Thermo Scientific™ MS data

system, such as Thermo Xcalibur™.

This guide also describes how to upgrade a Hewlett Packard™ 1100 Series LC to an

Agilent 1100 Series LC with Ethernet communication.

To suggest changes to the documentation or to the Help

Complete a brief survey about this document by clicking the button below. Thank you in

advance for your help.

Contents

•Related Documentation

•System Requirements

•Supported Firmware Versions

•Cautions and Special Notices

•Contacting Us

Preface

vi Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

Related Documentation

In addition to this guide, you can also access the following documents as PDF files from the

data system computer.

To view the product manuals

From the Microsoft™ Windows™ taskbar, do the following:

• For an LC instrument (LC Devices 2.2 or later), choose Start > All Programs >

Thermo Instruments > Manuals > LC Devices > Product Family, and then click

the PDF file that you want to view.

• For the Xcalibur manual set, choose Start > All Programs > Thermo Xcalibur >

Manuals > Xcalibur.

• For a Thermo Scientific mass spectrometer, choose Start > All Programs > Thermo

Instruments and so on.

For access to the application Help, follow this procedure.

To view application-specific Help

• From the Xcalibur Instrument Setup window, choose Help > Agilent 1100 Help.

• If information about setting parameters is available for a specific view, page, or dialog

box, click Help or press the F1 key for information about setting parameters.

Preface

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices vii

System Requirements

Ensure that the system meets these minimum requirements.

IMPORTANT Before you install the device drivers, ensure that the data system computer

has a compatible version of the Thermo Foundation™ platform as noted in the Thermo LC

Devices x.x.x Release Notes.

Table 1. System requirements

System Minimum requirements

Computer • 2 GHz processor with 1 GB RAM

•DVD drive

• 80 GB available on drive C

• Video card and monitor capable of 1280 × 1024 resolution

• Network interface cards, two

•NTFS format

Software • Adobe™ Reader™ 9.0

• Microsoft™ Windows™ operating system:

Windows 7 Professional (32-bit and 64-bita)

Windows XP Workstation SP3

• Thermo Scientific software:

a LC Devices 2.8.0 and later are compatible with Windows 7, 32-bit and 64-bit

LC Devices Foundation

2.6.0 and later 1.0.2 SP2 and later

2.5.0 SP3 1.0.2 SP2

2.5.0 SP1 or SP2 2.0

2.5.0 1.0.2 SP2

2.2.1–2.4 1.0 to 1.0.2 SP1 only

Preface

viii Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

(Windows 7 only) If you receive a server failure error when you try to open the Xcalibur

Instrument Setup window, follow the next procedure.

To resolve a server failure for the Xcalibur data system

1. Verify that the installed versions of Thermo Foundation and LC Devices are compatible

(see Table 1).

2. If the installed LC Devices software is compatible with Foundation, go to step 3. If it is

not compatible, do the following:

a. Use the Windows Control Panel to uninstall all of the modules from LC Devices.

b. Install the compatible version of LC Devices.

c. Restart the data system computer.

3. If the installed LC Devices software is compatible with Foundation, do the following:

a. Open the Windows Control Panel.

b. In the top Search box, type Change User Account Control Settings, and then select

this link to open the User Account Control Settings dialog box.

c. Move the slider to the Default position (Figure 1).

d. Click OK.

Figure 1. User Account Control Settings dialog box

Slider set at the

Default position

Preface

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices ix

Supported Firmware Versions

LC Devices supports the model numbers and firmware versions of the Agilent 1100 Series

modules listed in Tabl e 2 . For instructions on how to check the firmware version, see

Chapter 1, “Checking the Agilent Firmware Versions.”

Cautions and Special Notices

Make sure you follow the cautions and special notices presented in this guide. Cautions and

special notices appear in boxes; those concerning safety or possible system damage also have

corresponding caution symbols.

This guide uses the following types of cautions and special notices.

Table 2. Supported firmware versions for the Agilent 1100 Series LC

Module Model number Firmware version

Autosampler G1313A A.06.01 [012]

Thermostatted Autosampler G1329A A.06.01 [012]

Well-plate Autosampler G1367A A.06.01 [012]

Thermostatted Column Compartment G1316A A.06.01 [012]

Diode Array Detector G1315A A.06.01 [012]

Multiple Wavelength Detectora

a Only works with version B of the Control Module.

G1365B A.06.01 [012]

Variable Wavelength Detector G1314A A.06.01 [012]

Binary Pump G1312A A.06.01 [012]

Quaternary Pump G1311A A.06.01 [012]

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

xAgilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

The following additional caution-specific symbol appears in the Agilent 1100 Series Getting

Connected Guide for LC Devices.

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

To contact Technical Support

To contact Customer Service for ordering information

To copy manuals from the Internet

Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click

Customer Manuals in the left margin of the window.

To suggest changes to the Help

• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

CAUTION Highlights electric shock-related hazards to human beings. Each electric shock

notice is accompanied by the international High Voltage symbol.

Phone 800-532-4752

Fax 561-688-8736

E-mail us.techsupport.analyze@thermofisher.com

Phone 800-532-4752

Fax 561-688-8731

E-mail us.customer-support.analyze@thermofisher.com

Web site http://www.thermoscientific.com/en/support-landing/support.html

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices 1

Checking the Agilent Firmware Versions

This chapter describes how to check the compatibility of the Agilent 1100 Series LC system

with the device drivers provided on the Thermo Scientific LC Devices software DVD.

To check the firmware version of the LC module

1. Make sure that the modules are connected by controller area network (CAN)

communication cables, that all the modules are turned on, and that the handheld

Agilent 1100 Control Module is connected.

For instructions on how to connect the CAN cables, refer to the Agilent 1100 Series LC

reference manuals.

2. On the Control Module, press the ESC key until System appears in the upper left corner

of the LCD display.

3. On the Control Module, press F4 to access the Records LCD display (Figure 2).

Figure 2. Records LCD display

4. Compare the firmware versions to those in Table 2 on page ix.

IMPORTANT When you are done, to prevent data system failure, disconnect the

handheld Control Module cable from the LC stack.

Records

Module Product Serial# Version On-time EMF

Time Ready

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices 3

Connecting the Hardware

This chapter describes how to install the interface boards, connect the Ethernet cables,

and make the contact closure connections required to control an LC/MS

(Agilent LC/Thermo Scientific MS) system from the Xcalibur data system.

Ethernet Communication Kit

To connect the Agilent LC system to a Thermo Scientific mass spectrometer, you must have

the following parts:

• Ethernet Communication Kit (Table 3)

• JetDirect™ 400N LAN card (P/N 00825-01140) or an Agilent LAN card

(Model G1369A)

• Agilent binary-coded decimal (BCD) interface board for contact closure

Contents

•Ethernet Communication Kit

•Installing a Network Card in an Agilent Module

•Connecting the Ethernet Cables

•Installing the External Contact Interface Board

•Connecting the Contact Closure Cable

Table 3. Ethernet Communication Kit (P/N OPTON 30012)

Description Part number

Agilent BCD (contact interface) board 00012-27714

Contact closure cable with 15-pin connector 00012-27716

Ethernet cable, straight shielded Cat5, 3 m (10 ft) 00012-70008

Fast Ethernet switch, 10T/100Base-TX, 5-port 00825-01-00024

2 Connecting the Hardware

Installing a Network Card in an Agilent Module

4Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

Installing a Network Card in an Agilent Module

For the Agilent LC system to communicate with the Xcalibur data system through an

Ethernet connection, one of the modules in the Agilent 1100 Series LC stack must have a

network interface card. If the stack has a detector, the card is usually installed in the detector.

Agilent 1100 Series LC systems with JetDirect network cards must have revision 2 main

boards to function properly with LAN communications. Tab l e 4 lists the required main board

and its starting serial number for each module.

Tip Vendors label network cards with a unique MAC address, which is a 12-digit

hexadecimal number. The first portion of the MAC address identifies the vendor. The

second portion of the MAC address is a unique serial number. For example, a typical mark

on a network card is AD: 0030C1C49982, where 0030C1C49982 is the stack MAC

address. The first portion, 0030C1, identifies the vendor as Hewlett-Packard. The MAC

address of the HP JetDirect 400N network card is located on the back of the card.

Because you need the MAC address when you configure the Agilent stack, record the

network card MAC address before you install the card.

Table 4. Supported main board serial numbers for each module

Module P/N (Main board) Serial numbers for supported version a

a All serial numbers above the listed number in numeric order are supported.

G1310A

Isocratic pump

G1311-65520 DE64300355 (made in Germany)

US64400233 (made in the U.S.)

G1311A

Quaternary pump

G1311-65520 DE64301137 (made in Germany)

US64401134 (made in the U.S.)

G1312A

Binary pump

G1312-65520 DE64300703 (made in Germany)

US64400425 (made in the U.S.)

G1313A

ALS autosampler

G1313-65520 DE64302092 (made in Germany)

US64400886 (made in the U.S.)

G1314A

Variable wavelength detector

G1314-65520 JP64201926 (made in Japan)

G1315A

Diode array detector

G1315-65520 DE64301532 (made in Germany)

US64400333 (made in the U.S.)

2 Connecting the Hardware

Installing a Network Card in an Agilent Module

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices 5

To install the network interface card in an Agilent module

1. Make sure the module (usually the detector) is turned off and unplugged from line power.

Figure 3 shows the location of the power switches.

When the power switch of a module is in the On position, the LED on the switch is

green. When you turn the power switch of a module to the Off position, the LED

turns off.

If your LC stack contains a detector (for example, a diode-array detector, variable

wavelength detector, or multiple wavelength detector), install the network interface card

in the detector. If your LC stack does not contain a detector, install the network card in

any module with an open slot.

Figure 3. Power switches on the front of the Agilent 1100 Series modules

CAUTION To avoid electric shock, unplug the power cable that connects the Agilent

module to line power. After you turn the power switch to the Off position, the power

supply continues to draw current.

Note You can install an Agilent LAN interface board (Model G1369A) instead of a

JetDirect 400N network card.

Binary pump

Autosampler

Column

compartment

Detector

position

Off

position

2 Connecting the Hardware

Installing a Network Card in an Agilent Module

6Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

2. To prevent damage to the interface board, ensure that you are using protection from

electrostatic discharge (ESD). For more information on preventing damage caused by an

electric discharge, refer to the reference manual for the Agilent module.

3. To remove the cover plate from the slot where you plan to install the network interface

card, loosen the two screws that fasten the plate to the chassis of the module.

Figure 4 shows the location of the cover plate on the back panel of an Agilent 1100 Series

detector.

Figure 4. Back panel of an Agilent 1100 Series detector

4. Record the MAC address (12-digit hexadecimal number) located on the back of the

HP JetDirect 400N network card. You use this number to complete the configuration of

the Agilent stack (see Figure 9 on page 12).

5. Insert the HP JetDirect 400N network card into the slot, and then tighten the two screws

that fasten the PCB to the chassis of the module.

CAUTION To prevent damage to an instrument, always use ESD protection when

handling electronic boards and components.

ANALOG ANALOG RS232 RS232 CAN CAN

Cover plate

2 Connecting the Hardware

Connecting the Ethernet Cables

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices 7

Connecting the Ethernet Cables

To connect the Ethernet communication cable to an Agilent LC stack

1. Connect one Ethernet cable from the JetDirect 400N network card to the Ethernet

switch.

2. Connect the second Ethernet cable from the Ethernet switch to the Ethernet network

card in the data system computer that is dedicated to the LC/MS system (typically

network interface card number 3) (Figure 5).

Figure 5. Ethernet connections

CAUTION Safety and EMC regulations require the use of Category 5 shielded Ethernet

communication cables, maximum 3 m (10 ft) long.

HEWLETT

PACKARD

JET DIRECT 400N

BNC

100 (RJ-45)

HEWLETT

PACKARD

JET DIRECT 400N

USB

ENET

Computer

Dell™ Precision T3500

(subject to change)

JetDirect 400N card

(installed in one of the

Agilent modules)

(Not drawn to scale.)

Ethernet switch

Cat5 shielded

Ethernet cables

2 Connecting the Hardware

Installing the External Contact Interface Board

8Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

Installing the External Contact Interface Board

If your Agilent 1100 Series autosampler is not already equipped with an external contact

interface board, install one as described below.

To install the external contact interface board

1. If you have not already done so, turn off the autosampler and unplug it from line power.

2. To prevent damage to the interface board, ensure that you are using ESD protection. For

more information on preventing damage caused by an electric discharge, refer to the

reference manual for the Agilent module.

3. To remove the cover plate from the slot where you plan to install the external contact

interface board, loosen the two screws that fasten the plate to the chassis of the

autosampler.

4. Insert the external contact interface board into the slot (see Table 3 for the external

contact interface board part number). Tighten the two screws to fasten the board to the

chassis of the autosampler.

Figure 6 shows an installed external contact interface board.

Figure 6. External contact interface board (BCD board) installed in an Agilent autosampler

CAUTION To prevent personal injury caused by an electric shock, always turn off the

Agilent module and unplug its power supply cord from the electrical outlet before

removing the cover.

CAUTION To prevent damage to an instrument, always use ESD protection when

handling electronic boards and components.

1 8

Screws that secure the cover plate

VIAL- # -OUTPUT RELAY CONTACTS

REMOTE RS232 CAN CAN HP-18 CONFIG

2 Connecting the Hardware

Connecting the Contact Closure Cable

Thermo Scientific Agilent 1100 Series Getting Connected Guide for LC Devices 9

Connecting the Contact Closure Cable

An external contact closure (trigger) cable relays the start signal from the Agilent LC module

to the Thermo Scientific mass spectrometer. One end of the cable has a DB15 connector and

the other end has a set of exposed wires.

Figure 7 shows the contact closure cable set up for a Thermo Scientific TSQ™ Series mass

spectrometer with a 2-pin connector. For ordering information, see “Ethernet

Communication Kit” on page 3.

Figure 7. Contact closure cable set up with a 2-pin connector

To connect the contact closure cable to the mass spectrometer

1. Follow the procedure “To install the external contact interface board” on page 8.

2. Using an appropriate connector for the mass spectrometer, connect the white wire from

the contact closure cable to the Start In+ pin and the brown wire to the Start In– pin on

the mass spectrometer’s input/output (I/O) panel (Figure 8).

3. Connect the cable’s DB15 plug to the RELAY CONTACTS socket located on the LC

module’s installed contact interface board (Figure 8).

Note The instrument control platform for the Thermo Scientific mass spectrometer must

be compatible with the LC Devices instrument control application, and the instrument

control application must be compatible with the Thermo Scientific application installed

on your data system computer. See the LC Devices Release Notes.

2-pin connector supplied

with the mass spectrometer

DB15 connector DB15 connector

2 Connecting the Hardware

Connecting the Contact Closure Cable

10 Agilent 1100 Series Getting Connected Guide for LC Devices Thermo Scientific

Figure 8. Contact closure connections between the Agilent contact interface board and the Thermo Scientific MS

START IN

Start In +

Start In

START IN

(5V Max)

–

(right-side

panel)

(left-side

panel)

White wire

Brown wire

TSQ Series MS

(back panel)

LCQ Fleet™,

LTQ™ Series, or

LXQ™ MS

(right-side panel)

Surveyor™

MSQ™ MS

(back panel)

LCQ™ Series MS

(back panel)

(Not drawn to scale.)

Contact closure cable

connects to the relay contacts

on the contact interface board.

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Model: Agilent 1100 Series

Type: User guide

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Thermo Fisher Scientific Agilent 1100 Series User guide

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