Richard-Allan Scientific

Subsidiary of Thermo Fisher Scientific

4481 Campus Drive, Kalamazoo, MI 49008

Phone: 1 (800) 522-7270

Phone: + 1 (269) 544-5600

www.thermofisher.com/pathology

Anatomical Pathology

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Thermo Shandon Limited

Subsidiary of Thermo Fisher Scientific

Tudor Road, Manor Park,

Runcorn, Cheshire WA7 1TA, UK

Phone: +44 (0) 1928 534 050

Fax: + 44 (0) 1928 534 049

processing and staining of histological and cytological specimens. The products are a clear, colorless blend of

ethyl, isopropyl, and methyl alcohol that in some cases have trace amounts of other denatured materials. All

Dehydrant Alcohol products are non-controlled substances and do not require record keeping. The formulations

are readily miscible with water to form any desired grade of alcohol and can be used with all clearing reagents.

The Dehydrant Alcohol line can be used with both open and closed tissue processors. Protective gloves should

be worn and work should be performed in a ventilated area. Dehydrant Alcohol is a ammable product and must

be stored in a ammable re cabinet. The Dehydrant Alcohol product line can also be used for hydrating and

dehydrating tissue sections during staining. They can be used with all special stains that require dehydration, as

solvents for preparing special stains, and immunohistochemical procedures that are compatible with alcoholic

dehydrants. The Dehydrant Alcohol product line can be substituted in place of any alcohol you are presently using

in the cleaning cycle of a closed tissue processor in an anhydrous concentration.

The laboratory should develop a product rotation and change out schedule that adheres to the policies of their

The following tissue processing schedule is recommended for the average hospital surgical load.

Station Solution Time

1 10% Neutral Buffered Formalin holding

2 10% Neutral Buffered Formalin 1 hour

3 Pen-Fix™ or 80% Dehydrant 40 minutes

4 95% Dehydrant 40 minutes

11 Parafn 1 hour

12 Parafn 1 hour

Note: This procedure may not t every situation. Modications may be necessary.

When small biopsy or thin tissue specimens (less than 2 mm in thickness) are processed separately, the

following tissue processing schedule is recommended. Tissues are assumed to be xed. If not, stations 1 and 2

should utilize 10% Neutral Buffered Formalin for a minimum of 30 minutes each.

Station Solution Time

1 10% Neutral Buffered Formalin (30 minutes)

2 10% Neutral Buffered Formalin (30 minutes)

3 Pen-Fix or 80% Dehydrant 10 minutes

10 Clearing Reagent 15 minutes

11 Parafn 20 minutes

12 Parafn 20 minutes

Note: This procedure may not t every situation. Modications may be necessary.

Staining Instructions for Use

It is recommended that three stations of 100% Dehydrant Alcohol for 1 minute each follow the clearing reagent

after deparafnization, and at least one station of 95% Dehydrant Alcohol be used prior to the water rinse.

After staining is completed, slides should be dehydrated in three stations of 100% Dehydrant Alcohol for 1 minute

each before clearing. This will assure complete dehydration, which results in maximum slide clarity.

A 95% Dehydrant Alcohol can be used prior to the eosin-y to eliminate water carryover into the stain. Another

1 Clearing Reagent 3 minutes

2 Clearing Reagent 3 minutes

3 Clearing Reagent 3 minutes

4 100% Dehydrant 1 minute

5 100% Dehydrant 1 minute

6 100% Dehydrant 1 minute

7 95% Dehydrant 1 minute

8 Rinse in running tap water Briey

9 Deionized or distilled water Rinse

10 Hematoxylin Chosen Time

17 Eosin-Y Chosen Time

18 100% Dehydrant 1 minute

19 100% Dehydrant 1 minute

20 100% Dehydrant 1 minute

21 Clearing Reagent 1 minute

22 Clearing Reagent 1 minute

23 Clearing Reagent 1 minute

Note: This procedure may not t every situation. Modications may be necessary.

Warnings and Precautions

See Safety Data Sheets for warnings and precautions, as well as EUH code denitions.

Dehydrant Alcohol 100% 5 L 4/cs. 6215SS

Dehydrant Alcohol 95% 1 gal. (3.79 L) bottle 4/cs. 6301

Dehydrant Alcohol 95% 5 L 4/cs. 6315

Dehydrant Alcohol 95% 55 gal. (208 L) drum 2/cs. 6355

Dehydrant Alcohol 80% 1 gal. (3.79 L) bottle 4/cs. 6401

Dehydrant Alcohol 80% 5 L 4/cs. 6415

Dehydrant Alcohol 70% 1 gal. (3.79 L) bottle 4/cs. 6501

Dehydrant Alcohol 70% 5 L 4/cs. 6515

* For further instructions on how to use the Syntri Safeguard products please consult the Thermo Scientic Revos

user’s manual