National DM-52 Instructions Manual

Brand: National

Model: DM-52

Category: Microscopes

Type: Instructions Manual

National Optical & Scientific Instrument Inc.

6508 Tri-County Parkway

Schertz, Texas 78154

Phone (210) 590-9010 Fax (210) 590-1104

INSTRUCTIONS FOR

MODEL DM-52

COMPOUND BIOLOGICAL MICROSCOPE

WITH DIGITAL CAMERA

(For microscope operation only. Camera operation covered in separate

supplement on disc.)

HOW TO USE YOUR MICROSCOPE SERIAL NUMBERS

1. Microscope serial number

: This number (etched on inside of small ledge

immediately behind stage plate) is the number under which your warranty

is registered.

2. Microscope DM number: This number (found on a white sticker on the

bottom of the microscope) is used for logging on the Motic web site, which

gives you the ability to download free software upgrades. This number is

also used to register the software when loading on the computer for the

first time.

National Optical & Scientific Instrument Inc.

LED indicator light

Arm

Safety rack stop

Focusing knob

Rubber feet

On/Off switch with

rheostat intensity control

LED illuminator

Rotating stage

In stage lens

Objective lens

Objective turret (nosepiece)

Eyepiece tube

Eyepiece

(ocular lens)

DC recharging

socket

USB port

Stage clips

About the Digital Microscope

The manual for your new digital microscope is in two parts. This first part describes the basic

nomenclature and functions of the microscope, which can be used as a fully functional microscope,

independent of the camera. The second part is the Motic Image Quick Start Guide located on you CD,

which provides detailed documentation for installation and operation of the Motic software. In order to

achieve optimum results, it is important that you carefully read these documentation manuals before

operating your microscope or camera.

UNPACKING

1. Your microscope is packed with the following components, all of which have been checked at the

factory. Carefully remove all components and check against this list. Retain styrofoam container in

case microscope must be transported or returned to factory for any reason.

A. Microscope, with three objective lenses already installed.

B. WF 10x eyepiece.

C. Instruction manual

D. Motic Educator CD

E. Motic Play CD.

F. Calibration slide

G. Automatic switching recharger operates on 100 to 240 volts AC 50/60 Hz.

H. USB cable (for connecting microscope to computer)

I. 0.9mm “L” type hex key wrench (for locking eyepiece and replacing LED lamp)

J. Dustcover

K. Warranty card

2. If it becomes necessary to ship the microscope for any reason, repack it in the styrofoam container,

and then pack the styrofoam in another corrugated shipping container for optimum protection. Use of

the styrofoam alone will not provide adequate protection in transit, and will void your warranty.

DESCRIPTION OF COMPONENTS

1. LED INDICATOR LIGHT: Indicates if camera is on. This light will not come on, nor will this light be

on, until microscope is connected to computer with USB cable and software activated. This

connection occurs later, and is covered in software manuals.

2. EYEPIECE (ocular lens): Lens closest to the eye, magnifies the primary image formed by the

objective lens.

3. EYEPIECE TUBE: Tube with locking setscrew, holds and secures eyepiece to microscope.

4. OBJECTIVE TURRET (nosepiece): Revolving turret which holds objective lenses, permits changes of

magnification by rotating different powered objective lenses into optical path.

5. OBJECTIVE LENS: Lens closest to the object being viewed, forms first magnified image of the

specimen.

6. STAGE CLIPS: Two locked-on clips hold specimen slide in place on stage.

7. ROTATING STAGE: Platform of the microscope where the specimen slide is placed. Stage rotates

360 degrees and glides left to right permitting easy manipulation of the specimen slide.

8. IN STAGE LENS: A specially designed diffuser lens, fixed in center of stage, diffuses light rays from

LED illuminator to evenly fill the back lens element lens to improve image resolution.

9. SAFETY RACK STOP: When properly adjusted, controls maximum upward travel of stage. Prevents

higher power objectives from breaking specimen slides, prevents damage to objective lenses. This

stop has been pre-adjusted at the factory.

10. FOCUSING KNOBS: Focusing knobs located on each side of arm, raise or lower stage to bring

specimen image into focus. .

11. LED ILLUMINATIOR: Built-in substage LED illuminator provides constant, reliable pre-focused

illumination equal to a 20-watt tungsten bulb. Powered by 3 rechargeable AA nickel metal hydride

batteries, no power outlet or electric cord are needed.

13. ON/OFF SWITCH WITH RHEOSTAT INTENSITY CONTROL This turns microscope lamp on and

controls the intensity of the illumination.

14. RECHARGER: Automatic switching charger used to recharge batteries.

15. USB CABLE: Connects microscope & camera to computer. .

ASSEMBLY

1. Remove plastic covering from eyepiece tube. Using the 0.9 “L” type hex key wrench loosen (but do

not remove) the small hex socket set screw located beneath the top of eyepiece tube.

2. Unwrap eyepiece, making certain that lens surfaces do not come in contact with dust, dirt or

fingerprints. Damage to optical surfaces can result from such contaminants, and reduce image quality.

3. Carefully insert the WF 10 x eyepiece into eyepiece tube until it is fully seated on top of eyepiece tube.

Using the 0.90 “L” type hex key wrench slowly tighten the small hex socket set screw located beneath

the top of eyepiece tube. Socket set screw fits into special groove located on lower sleeve of

eyepiece. Tighten screw until you are unable to remove eyepiece from tube but allowing the eyepiece

to rotate.

OPERATION

Your microscope is fully functional as a standard microscope. The following instructions apply only to

operation of the microscope. Refer to the software instructions for operation of the camera. Some steps

for microscope operations are altered slightly in the software documentation, in order to utilize some of the

unique features provided by the digital camera and software.

1. Place microscope directly in front of you in a manner that permits you to comfortable look into the

eyepiece and easily reach the focusing controls.

2. It is recommended that you charge the batteries before initial use and after prolonged storage as the

batteries may have discharged. Plug output cord from battery charger into DC recharging socket

located on back of microscope base. Your automatic switching recharger operates on 100 to 240

volts AC 50/60 Hz. Plug recharger into your AC wall outlet. Battery recharger is also equipped with an

automatic “trickle charge” feature, the red LED indicator lamp located on recharger will be illuminated

when batteries are receiving maximum charge. After batteries are charged, the red LED indicator

lamp will turn to green and charger automatically switches to “trickle charge”. The charger can be left

plugged in, but for safety reasons it is a good idea to disconnect the charger from the AC wall outlet

and the output cord from recharging socket after 12 hours. Batteries and charger may feel warm

when charging, and unplugging the recharger is a safety precaution.

3. Your Digital LED microscope can be used during recharging. Simply rotate the ON/OFF switch and

rheostat control knob until the LED lamp is on and proceed as follows.

4. Rotate focus knobs to move stage down (away) from objective lenses as far as possible.

5. Turn the objective turret until the 4x (smallest) objective lens “clicks” into position in the optical path.

Each time that you change from one objective lens to another you should turn the turret until you hear

the “click”, which indicates that the lens is properly indexed in the optical path.

6. Place specimen slide, cover slip facing up, on stage with specimen centered over condenser lens in

middle of stage.

7. While looking through the eyepiece, rotate focusing knobs until specimen comes into focus. If image

does not appear in field of view, move gliding stage slightly until image appears in field of view.

8. Changing magnification.

A. Rotating objective turret until different objective lens is moved into optical path changes

magnification. Always turn turret until you hear the “click”, indication that lens is properly indexed.

Otherwise, you will not be able to see anything when looking through the microscope.

B. See chart below for magnifications provided with your microscope.

MICROSCOPE SPECIFICATION CHART

OBJECTIVE

COLOR CODE RING

MAGNIGICATION

with WF 10X Eyepiece

Red

40X

10x

Yellow

100X

40X

Blue

400X

C. Also note that each objective has a color ring, which permits you to instruct changes in

magnification by referring to an easily observed color rather than to a number.

D. Microscope has been parfocalled at the factory, which allows easy change from one magnification

to another, requiring only a small adjustment of the focus knobs.

E. As magnification is increased, the field of view (area of specimen seen through the microscope)

will decrease. That is why it is easier to find the specific area of interest on the specimen by

starting with the lowest 4x objective lens, before increasing magnification with the 10x or 40x

objective lens.

NOTE: Care must be taken when rotating the 40x objective into place. This lens has a spring

retractable mechanism, which retracts slightly into its housing if the front of the lens, strikes the

specimen slide. The rack stop has been adjusted at the factory to assure the 40x lens will clear

the thickness of a normal specimen slide and cover slip. However, if the rack stop has been

improperly adjusted, or if using a thicker than normal slide or cover slip, moving the 40x lens too

quickly or carelessly could cause damage to the front lens element or to the slide.

F. Do not let the front lens element come into contact with a wet slide surface, as any contact with

any moisture could damage the lens. If lens is exposed to moisture, promptly wipe with soft tissue

to remove moisture.

MAINTENANCE

WARNING: For your own safety, turn switch off and remove recharger from power source before

maintaining your microscope. If the power cord is worn, cut or damaged in any way, have it replaced

immediately to avoid shock or fire hazard.

1. OPTICAL MAINTENANCE

A. Do not attempt to disassemble any lens components. Consult a microscope service technician

when any repairs not covered by instructions are needed.

B. Prior to cleaning any lens surface, brush dust or lint off lens surface using a camel hairbrush. You

can also use an ear syringe or canned compressed air, such as that sold by most computer

stores.

C. Do not remove eyepieces or objective lenses to clean. Clean only the outer lens surface. Breath

on lens to dampen surface, then wipe with lens paper, or use a cotton swab moistened with

distilled water. Wipe lenses with a circular motion, applying as little pressure as possible. Avoid

wiping dry lens surface as lenses are scratched easily. If excessive dirt or grease gets on lens

surfaces, a small amount of Windex can be used on a cotton swab or lens tissue. To clean

objective lenses, do not remove objectives from microscope. Clean front lens element only,

following same procedure.

NOTE: Fingerprints or other matter on the front lens element of the objective lens is the single

most common reason that you will have difficulty in focusing the microscope. Before having costly

servicing done, or before returning to National for “warranty repair”, make certain to examine the

front lens element with a magnifying glass or eye loupe for the presence of such contaminants. If

a microscope is returned for warranty repair, and it is determined that such contaminants are the

problem, this is not covered under warranty and National will submit a cost estimate for cleaning.

2. MECHANICAL MAINTENANCE

A. The rack stop screw has been pre-adjusted at the factory and should not require re-adjustment.

B. Metal parts: Use a clean, damp cloth to remove dust or dirt from metal parts, followed by a dry

cloth.

3. ELECTRICAL MAINTENANCE

The extent of electrical maintenance, by other than a qualified technician, should be LED lamp

replacement, battery recharging and battery replacement. Before maintenance, be sure that

recharger is not connected to microscope.

A. Recharging batteries: Plug output cord from battery charger into DC recharging socket located on

back of microscope base. Your automatic switching recharger operates on 100 to 240 volts AC

50/60 Hz, plug recharger into your AC wall outlet. The red LED indicator lamp located on

recharger will be illuminated when batteries are receiving maximum charge. After batteries are

charged, the red LED indicator lamp will turn to green and charger automatically switches to

“trickle charge”. The charger can be left plugged in, but for safety reasons it is a good idea to

disconnect the charger from the AC wall outlet and the output cord from recharging socket after

12 hours. Batteries and charger may feel warm when charging, and unplugging the recharger is a

safety precaution. You may operate the microscope light even while it is being recharged. Simply

continue to use the microscope while the recharger is fully engaged.

B. Replace batteries: Your microscope includes 3 rechargeable AA nickel metal hydride batteries.

These may be recharged up to 500 times, but if you observe that a recharge is providing

significantly less than 40 hours of operation it is probably time to replace batteries.

a) Gently lay microscope on its side. Remove four rubber feet that secure cover plate to base

and carefully move plate to the side (Do not place excessive stress on USB cable assembly).

Using a small Phillips screwdriver, carefully remove Phillips screw that holds battery case

together. Slide lid of case straight out to remove and expose batteries. Remove all 3

batteries and replace with new rechargeable AA nickel hydride batteries, making certain with

correct polarity according to markings on battery holder. Replace battery case lid, Philips

screw, cover plate and 4 rubber feet.

b) IMPORTANT WARNING: DO NOT USE REGULAR ALKALINE BATTERIES IN THIS

MICROSCOPE. ANY ATTEMPT TO RECHARGE ALKALINE TYPE BATTERIES COULD

RESULT IN BATTERIES EXPLODING.

c) Follow instruction on new battery packaging to determine if they are already charged, or if

they should be charged before initial use. If recharging is required follow directions in “3. A.”

above.

C. Replacing LED lamp element: A LED “lamp” will last up to 50,000 hours, so you don’t have to do

this exercise very often.

a) Using hex wrench supplied with your microscope, loosen two hex socket set screws located

on the lower part of illuminator field lens housing. Remove field lens housing to expose LED

“lamp” Remove lamp by grasping the plastic base of bulb and gently pull straight up. Insert

new LED “lamp” (Note lamp has a base designed to properly orientate this LED element in

socket)

b) Replace illuminator field lens housing and tighten the two hex socket set screws.

TROUBLESHOOTING

PROBLEM

REASON FOR PROBLEM

SOLUTION

Light fails to operate.

Batteries fully discharged.

Recharge batteries.

Batteries fully discharged and will

not recharge.

Light switch in OFF position

Replace batteries of replace

charger.

Turn light switch to ON position

12 volt DC output plug not

plugged into DC power jack.

Plug 12-volt DC output plug into

DC power jack.

Lamp burned out.

Replace lamp.

Image does not focus

Slide upside down.

Slide cover slip too thick.

Place slide on stage with cover

slip up.

Use 0.17mm thick cover slip

(No. 1 cover slip)

Poor resolution

(Image not sharp)

Objective lenses dirty

Eyepiece lens dirty.

Too much light

Clean objective lenses.

Clean eyepiece lenses.

Turn rheostat to decrease light

intensity.

Spots in field of view.

Eyepiece or condenser lens dirty.

Specimen slide dirty

Clean lens ***

Clean slide.

***Spots in field of view can also result from dirt on inside of eyepiece. It is recommended that you have

service technician clean inside of lens.

OPTIONAL ACCESSORIES AND PARTS:

#610-106 WF10x eyepiece w/pointer

#800-001 Replacement LED lamp

#802-003 Replacement auto recharger (input 100-240 volts AC, output 4.5 volts DC)

#975-001 Carrying case, anodized aluminum, fabric lining, accessory pocket. Interior dimension 14-

7/8 x 7-3/4 x 10-1/2 inches.

WARRANTY – 5 YEAR LIMITED WARRANTY

Please see our website, www.nationaloptical.com, for complete warranty details and exclusions.

Revised (11/6/2009)

National DM-52 Instructions Manual

Brand: National

Model: DM-52

Category: Microscopes

Type: Instructions Manual

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National DM-52 Instructions Manual

National DM-52 Instructions Manual

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