Thermo Fisher Scientific Waters LC User guide

Brand: Thermo Fisher Scientific

Model: Waters LC

Type: User guide

XCALI-97192 Revision B July 2007

Waters LC

LC Devices

Getting Connected

Waters® is a registered trademark and ACQUITY UPLC™ is a trademark of Waters Company.

Xcalibur™ is a trademark of Thermo Fisher Scientific Inc.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might

result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Revision A for LC Devices 2.02, Revision B for LC Devices 2.1.0. The instructions for

connecting the Waters 2795 Separation Module are backward compatible to LC Devices 2.0.2.

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug

Administration or other competent authorities.

Thermo Scientific Waters LC Devices Getting Connected iii

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v

About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi

Chapter 1 Connecting the Waters 2795 Separations Module . . . . . . . . . . . . . . . . . . . . . . . . . .1

Required Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Connecting the Contact Closure Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Connecting the Serial Communication Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

Setting Up the Separations Module for Remote Control . . . . . . . . . . . . . . . . . . . 5

Checking the Firmware Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Setting the Operating Mode to Remote Control . . . . . . . . . . . . . . . . . . . . . . . 6

Configuring the Waters 2795 Separations Module . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 2 Connecting a Waters Acquity LC System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Connecting the Waters Acquity LC to the Data System Computer . . . . . . . . . . 11

Installing and Configuring a Network Interface Card . . . . . . . . . . . . . . . . . . 11

Connecting the Ethernet Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Connecting the Contact Closure Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Configuring the Waters Acquity Devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Troubleshooting Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Starting the Waters Server Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Upgrading the Firmware of the Waters Acquity Devices . . . . . . . . . . . . . . . . 28

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Contents

Thermo Scientific Waters LC Devices Getting Connected v

Preface

About This Guide

This guide describes how to connect a Waters LC system to a Thermo Scientific mass

spectrometer and the data system computer.

Related Documentation

The Xcalibur LC Devices instrument control software provides Help.

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each

CAUTION notice is accompanied by an appropriate CAUTION symbol.

CAUTION Highlights electric shock related hazards to human beings. Each electric shock

notice is accompanied by the international High Voltage symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

vi Waters LC Devices Getting Connected Thermo Scientific

Contacting Us

There are several ways to contact Thermo Fisher Scientific.

YTo contact Technical Support

Find software updates and utilities to download at www.mssupport.thermo.com.

YTo contact Customer Service for ordering information

YTo suggest changes to documentation or to Help

• Fill out a reader survey online at www.thermo.com/lcms-techpubs.

• Send an e-mail message to the Technical Publications Editor at

techpubs.finnigan-lcms@thermofisher.com.

Phone 800-685-9535

Fax 561-688-8736

E-mail TechSupport.C+MS@thermofisher.com

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

Web site www.thermo.com/finnigan

Thermo Scientific Waters LC Devices Getting Connected 1

Connecting the Waters 2795 Separations Module

Required Parts

To connect the Waters 2795 Separations Module to a Thermo Scientific mass spectrometer,

order the Waters Interface kit from Thermo Fisher Scientific. This kit contains the cables

listed in Tab le 1. The serial communication cable connects the Waters 2795 Separations

Module to the data system computer. The contact closure (trigger) cable connects the Inject

Out pins of the separations module to the Start In pins on the I/O panel of the mass

spectrometer.

Connecting the Contact Closure Cable

If you want the separations module to trigger the start of data acquisition from a Thermo

Scientific mass spectrometer during a run, connect the Inject Out pins of the Waters 2795

Separations Module to the Start In pins on the I/O panel of the mass spectrometer.

Contents

•Required Parts

•Connecting the Contact Closure Cable

•Connecting the Serial Communication Cable

•Setting Up the Separations Module for Remote Control

•Configuring the Waters 2795 Separations Module

Table 1. Xcalibur kit used with the Waters 2795 Separations Module

Part Number Description of Kit

OPTON-21710 Waters Interface Kit

Serial communication cable with two DB9 connectors

2-wire contact closure cable, with an 8-pin connector and

a 2-pin connector

1 Connecting the Waters 2795 Separations Module

Connecting the Contact Closure Cable

2Waters LC Devices Getting Connected Thermo Scientific

YTo hardwire the Waters 2795 Separations Module to the MS detector

1. Connect the 8-pin connector of the 2-wire, contact closure cable (supplied in the Waters

Interface kit) to the contact closure terminal (labeled B) on the back panel of the Waters

2795 Separations Module.

2. Connect the 2-pin connector of the 2-wire, contact closure cable to the User I/O of the

MS detector. Follow the wiring scheme listed in Tabl e 2 and shown in Figure 1.

Table 2. Wiring the MS detector and the Waters 2795 Separations Module for contact closure

Waters 2795

Contact closure terminal B MS detector

Pin 1 Start In +

Pin 2 Start In -

1 Connecting the Waters 2795 Separations Module

Connecting the Contact Closure Cable

Thermo Scientific Waters LC Devices Getting Connected 3

Figure 1. Contact closure connection between the Waters 2795 Separations Module and the MS detector

Surveyor MSQ

(back panel)

Not drawn to scale

LCQ Fleet, LXQ, or LTQ

MS Detector

(right-side panel)

START IN

TSQ Series MS Detector

(back panel)

(right-side

panel)

Start In +

Start In -

START IN

(5V Max)

–

LCQ Series

(back panel)

(left-side

panel)

Peripheral

Control

RS232

Back panel of Waters 2795

White Wire

Brown Wire

White wire

Brown wire

(right-side

panel)

(right-side panel)

(left-side

panel)

(back panel)

Back panel of Waters 2795

Not drawn to scale

1 Connecting the Waters 2795 Separations Module

Connecting the Serial Communication Cable

4Waters LC Devices Getting Connected Thermo Scientific

Connecting the Serial Communication Cable

As Figure 2 shows, the data system computer communicates with the Waters 2795

Separations Module through an RS232 serial communication cable. This cable is provided in

the Waters Interface kit.

YTo connect the serial communication cable

1. Connect one end of the serial communication cable to the RS232 port on the back panel

of the Waters 2795 Separations Module. See Figure 2.

2. Connect the other end of the serial communication cable to an RS232 port on the data

system computer.

Figure 2. Serial communication connection

RS232

Computer

Waters 2795 Separations Module

(back panel)

Serial communication cable

(with two DB9 connectors)

1 Connecting the Waters 2795 Separations Module

Setting Up the Separations Module for Remote Control

Thermo Scientific Waters LC Devices Getting Connected 5

Setting Up the Separations Module for Remote Control

The Xcalibur based instrument control software for the Waters 2795 Separations Module is

compatible with firmware version 2.04. To control the Waters 2795 Separations Module, set

the separations module to operate by remote control through an RS232 connection to the

computer where Xcalibur resides.

To set up the separations module for remote control, follow these procedures:

•Checking the Firmware Version

•Setting the Operating Mode to Remote Control

Checking the Firmware Version

YTo check the firmware version for the Waters Separations Module

Turn on the instrument and watch the front panel LCD display as the instrument cycles

through the system startup.

At the end of the system initialization process the Main LCD display appears. See Figure 3.

The firmware version is listed in the lower-right corner of the display.

Figure 3. Main LCD display

Main

<Default> Idle

Separations Module

Press Menu/

Status for monitoring and direct control

User:

Revision 2.04

Develop

Methods Run

Samples Diag Config Log

01/02/2007 09:00:00 pm

Tester

Waters 2795

1 Connecting the Waters 2795 Separations Module

Setting Up the Separations Module for Remote Control

6Waters LC Devices Getting Connected Thermo Scientific

Setting the Operating Mode to Remote Control

YTo set up the Waters 2795 Separations Module for remote control

1. From the Main page on the LCD display of the Waters 2795 Separations Module, press

the Config screen key. See Figure 4.

Figure 4. Front panel LCD display and keypad for the Waters 2795 Separations Module

The Configuration page appears. See Figure 5.

Figure 5. Configuration page

2. Press the < > arrow key to highlight the System box, and then press the Enter key on the

front panel keypad.

123

456

789

Exit Menu/

Status

Stop

Flow

Enter

Clear

Display

Contrast

Arrow Keys

Conﬁg Key

Diag Config Log

Main

<Default> Idle

Separations Module

Press Menu/

Status for monitoring and direct control

User:

Revision 2.04

Develop

Methods Run

Samples Diag Config Log

01/02/2007 09:00:00 pm

Teste r

Waters 2795

Arrow keys

Config key

Configuration

<Default> Idle

Tester

Pressure Units:

500

Syringe Size: Loop Size:

System

Remote Control via RS-232

Printer Date Format: Alarm Sound:

User

Names

Detec-

tors Options

Set

Clock

Events

psi

MDY

Baud Rate:

1200

None Continuous

µL µL

IEEE 488 Address:

1 Connecting the Waters 2795 Separations Module

Configuring the Waters 2795 Separations Module

Thermo Scientific Waters LC Devices Getting Connected 7

The list of system options appears as shown in Figure 6.

Figure 6. Configuration page with the list of system options

3. Select Controlled via RS232 by pressing the < > and < > arrow keys, and then press

the Enter key on the front panel keypad to save the change.

4. Press the Exit key on the front panel keypad to return to the Main page.

Configuring the Waters 2795 Separations Module

To establish communication between the data system and the separations module, specify the

communication port to which you have connected the separations module. To make sure that

the separations module draws sample from the correct location, specify the plates you are

using.

YTo configure the Waters 2795 Separations Module

1. From the Windows XP taskbar, choose Start > All Programs > Xcalibur > Instrument

Configuration.

The Instrument Configuration dialog box appears. See Figure 7.

Configuration

<Default> Idle

Tester

Pressure Units:

500

Syringe Size: Loop Size:

System

Remote Control via RS-232

Printer Date Format: Alarm Sound:

User

Names

Detec-

tors Options

Set

Clock

Events

psi

MDY

Baud Rate:

1200

None Continuous

µL µL

IEEE 488 Address:

No Interaction

System Controller

Controlled by Millennium 32

Controlled by MassLynx

Controlled by Millennium 2.XX

Operate gradient by event in

Controlled via RS232

1 Connecting the Waters 2795 Separations Module

Configuring the Waters 2795 Separations Module

8Waters LC Devices Getting Connected Thermo Scientific

Figure 7. Instrument Configuration dialog box

2. Under Available Devices, double-click .

A copy of the Waters 2795 button appears under Configured Devices.

3. Under Configured Devices, double-click .

The Waters Alliance 2795 Configuration dialog box appears. See Figure 8.

1 Connecting the Waters 2795 Separations Module

Configuring the Waters 2795 Separations Module

Thermo Scientific Waters LC Devices Getting Connected 9

Figure 8. Alliance 2795 Configuration dialog box

4. In the Port list, confirm that the selected COM port is the RS232 port on the data system

computer to which you physically connected the separations module.

For information on connecting the serial communication cable, see “Connecting the

Serial Communication Cable” on page 3.

5. In the Carrier Plate boxes, specify the tray type that you are using and the sequential

reference counting order for each plate.

6. Click Autoconfigure, and then click OK to save the settings and close the dialog box.

The separations module is ready for remote control from the Xcalibur data system.

Port list

Thermo Scientific Waters LC Devices Getting Connected 11

Connecting a Waters Acquity LC System

Connecting the Waters Acquity LC to the Data System Computer

The Waters Acquity LC stack communicates with the data system computer through an

Ethernet connection. To make this connection, follow these procedures:

1. Installing and Configuring a Network Interface Card

2. Connecting the Ethernet Cables

Installing and Configuring a Network Interface Card

The Waters Acquity LC stack requires a dedicated network interface card.

YTo install an additional network interface card in the data system computer

Follow the instructions in the manuals provided by the manufacturer of the computer.

YTo set the IP address for the dedicated network interface card

1. To open the Internet Protocol (TCP/IP) Properties dialog box, do the following:

a. From the computer desktop, choose Start > Control Panel > Network Connections

> Local Area Connection.

The General page of the Local Area Connection Status dialog box appears.

Contents

•Connecting the Waters Acquity LC to the Data System Computer

•Connecting the Contact Closure Cable

•Configuring the Waters Acquity Devices

•Troubleshooting Communications

CAUTION To prevent personal injury caused by an electric shock, always unplug your

computer from the electrical outlet before removing the cover.

2 Connecting a Waters Acquity LC System

Connecting the Waters Acquity LC to the Data System Computer

12 Waters LC Devices Getting Connected Thermo Scientific

b. Click Properties.

The Local Area Connection Properties dialog box appears.

c. In the This connection uses the following items list, select Internet Protocol TCP/IP

and click Properties.

The Internet Protocol (TCP/IP) Properties dialog box appears. See Figure 9.

Figure 9. Internet Protocol (TCP/IP) Properties dialog box with the IP address for the

dedicated Ethernet card

2. Select the Use the following IP address option.

3. In the IP address box, type 192.168.0.1.

4. In the Subnet mask box, type 255.255.255.0.

5. Click OK to accept the new IP address and subnet mask, and exit the Internet Protocol

(TCP/IP) Properties dialog box.

6. Click OK to exit the Local Area Connection dialog box.

2 Connecting a Waters Acquity LC System

Connecting the Waters Acquity LC to the Data System Computer

Thermo Scientific Waters LC Devices Getting Connected 13

Connecting the Ethernet Cables

The Waters Acquity LC system comes with an Ethernet switch. Connect each device of the

Waters Acquity LC stack to the Ethernet switch, and connect the Ethernet switch to the data

system computer with category 5, shielded Ethernet cables.

YTo connect the Waters Acquity LC to the data system computer

1. Connect one end of a category 5, shielded Ethernet cable to the Ethernet switch supplied

with the Waters Acquity LC system.

2. Connect the other end of the category 5, shielded Ethernet cable to the Ethernet port of

the network interface card dedicated to the Waters Acquity LC system. See Figure 10.

Figure 10. Ethernet connections

CAUTION To ensure compliance with safety and EMC regulations, use the Ethernet

cables supplied in the Ethernet Communication kit or category 5, shielded Ethernet

cables no longer than 3 m (10 ft) in length.

Ethernet

III

Ethernet switch

Dedicated

network

interface card

2 Connecting a Waters Acquity LC System

Connecting the Contact Closure Cable

14 Waters LC Devices Getting Connected Thermo Scientific

Connecting the Contact Closure Cable

The LC system sends a ready signal after the Waters Acquity Sample Manager (autosampler)

makes an injection. If you want the Thermo Scientific mass spectrometer to start data

acquisition after the autosampler starts an injection, you must hardwire the output terminals

of the autosampler to the start terminals on the I/O panel of the mass spectrometer.

YTo connect the contact closure cable

1. Connect one end of the two-wire, contact closure cable in the Waters accessory kit to

pin 1 and pin 2 of the contact closure terminal (labeled I) on the back of the Waters

Acquity Sample Manager.

2. Connect the other end of the two-wire contact closure cable to the User I/O of the mass

spectrometer. Follow the wiring scheme listed in Table 3 and shown in Figure 11.

Table 3. Connecting the contact closure cable

Waters

Contact closure terminal I MS detector

Pin 1 Start In +

Pin 2 Start In -

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Thermo Fisher Scientific Waters LC User guide

Brand: Thermo Fisher Scientific

Model: Waters LC

Type: User guide

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Thermo Fisher Scientific Waters LC User guide

Thermo Fisher Scientific Waters LC User guide

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