Thermo Fisher Scientific Waters 2795 Separations Module User guide

Brand
Thermo Fisher Scientific
Model
Waters 2795 Separations Module
Type
User guide
Waters 2795
Separations Module
Getting Connected Guide
XCALI-97220 Revision B February 2011
© 2011 Thermo Fisher Scientific Inc. All rights reserved.
Xcalibur is a registered trademark of Thermo Fisher Scientific Inc. in the United States.
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countries.
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All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history: Revision A, January 2009; Revision B, February 2011.
Software versions: Xcalibur 2.1 or later; LC Devices 2.1.0 or later.
For Research Use Only. Not for use in diagnostic procedures.
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide iii
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi
Chapter 1 Connecting the Hardware Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Required Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Connecting the Contact Closure Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Connecting the Serial Communication Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chapter 2 Setting the Operating Mode to Remote Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
Chapter 3 Adding the Waters 2795 Separations Module to the Software
Configuration9
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Contents
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide v
P
Preface
This guide describes how to connect a Waters™ 2795 Separations Module to a Thermo
Scientific mass spectrometer (MS detector) and the data system computer.
To suggest changes to documentation or to Help
Complete a brief survey about this document by clicking the link below.
Thank you in advance for your help.
Related Documentation
In addition to this guide, the Waters ACQUITY instrument control application provides
Help. See Chapter 4, “Accessing Waters ACQUITY Help.”
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
CAUTION Highlights electric shock related hazards to human beings. Each electric shock
notice is accompanied by the international High Voltage symbol.
Preface
vi Waters 2795 Separations Module Getting Connected Guide Thermo Scientific
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
To contact Technical Support
Find software updates and utilities to download at mssupport.thermo.com.
To contact Customer Service for ordering information
To get local contact information for sales or service
Go to www.thermoscientific.com/wps/portal/ts/contactus.
To copy manuals from the Internet
Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click
Customer Manuals in the left margin of the window.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
Phone 800-532-4752
Fax 561-688-8736
E-mail us.techsupport.analyze@thermofisher.com
Knowledge base www.thermokb.com
Phone 800-532-4752
Fax 561-688-8731
E-mail us.customer-support.analyze@thermofisher.com
Web site www.thermo.com/ms
Preface
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide vii
To suggest changes to documentation or to Help
• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.
• Send an e-mail message to the Technical Publications Editor at
techpubs-lcms@thermofisher.com.
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 1
1
Connecting the Hardware Cables
Required Parts
To connect the Waters 2795 Separations Module to a Thermo Scientific mass spectrometer,
order the Waters Interface Kit from Thermo Fisher Scientific. This kit contains the cables
listed in Table 1. The serial communication cable connects the Waters 2795 Separations
Module to the data system computer. The contact closure (trigger) cable connects the Inject
Out pins of the separations module to the START IN pins on the input/output (I/O) panel of
the mass spectrometer.
Contents
•Required Parts
•Connecting the Contact Closure Cable
•Connecting the Serial Communication Cable
Table 1. Xcalibur kit for the Waters 2795 Separations Module
Part number Description of kit
OPTON-21710 Waters Interface Kit:
• Serial communication cable with two DB9 connectors
• 2-wire contact closure cable, with an 8-pin connector and a 2-pin
connector
1 Connecting the Hardware Cables
Connecting the Contact Closure Cable
2Waters 2795 Separations Module Getting Connected Guide Thermo Scientific
Connecting the Contact Closure Cable
To trigger the start of data acquisition with the Waters 2795 Separations Module, connect the
Inject Out pins of the Waters 2795 Separations Module to the START IN pins on the I/O
panel of the mass spectrometer.
To conect the Waters 2795 Separations Module to the MS detector
1. Connect the 8-pin connector of the 2-wire, contact closure cable to the contact closure
terminal (labeled B) on the back panel of the Waters 2795 Separations Module.
2. Connect the 2-pin connector of the 2-wire, contact closure cable to the START IN pins
of the MS detector, following the wiring scheme listed in Table 2 and shown in the
Figure 1 examples.
Table 2. Contact closure cable connections
Waters 2795
Contact closure terminal B MS detector
Pin 1 START IN +
Pin 2 START IN –
1 Connecting the Hardware Cables
Connecting the Contact Closure Cable
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 3
Figure 1. Contact closure connection between the Waters 2795 Separations Module and MS detectors
Surveyor
MSQ Plus
MS detector
+ -
LTQ Series
MS detector
START IN
START IN
TSQ Series
MS detector
START IN +
START IN -
START IN
(5V Max)
(5V Max)
+
– LCQ Series (Legacy)
MS detector
Peripheral
Control
B A
RS232
B A
White Wire
Brown Wire
White wire
Brown wire
(right-side
panel)
(right-side panel)
(left-side
panel)
(back panel)
(back panel)
(back panel)
Back panel of Waters 2795
Not drawn to scale
1 Connecting the Hardware Cables
Connecting the Serial Communication Cable
4Waters 2795 Separations Module Getting Connected Guide Thermo Scientific
Connecting the Serial Communication Cable
As Figure 2 shows, the data system computer communicates with the Waters 2795
Separations Module through an RS232 serial communication cable (Ta b le 1 on page 1).
To connect the serial communication cable
1. Connect one end of the serial communication cable to the RS232 port on the back panel
of the Waters 2795 Separations Module (Figure 2).
2. Connect the other end of the serial communication cable to an RS232 port on the data
system computer (Figure 2).
Figure 2. Serial communication connection
RS232
B A
Computer
Waters 2795 Separations Module
Back panel
Serial communication cable
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 5
2
Setting the Operating Mode to Remote Control
For the Waters 2795 Separations Module, firmware version 2.04 is compatible with the
Xcalibur-based instrument control application. To control the Waters 2795 Separations
Module, set the separations module to operate by remote control through an RS232
connection to the data system computer as described in the following procedure.
To check the firmware version of the Waters 2795 Separations Module and set the
operating mode to remote control
1. Turn on the Waters instrument and watch the front panel LCD display as the instrument
cycles through the system startup.
At the end of the system initialization process, the Main LCD display appears (Figure 3).
The firmware version appears in the lower-right corner of the display.
Figure 3. Main LCD display
Main
<Default> Idle
Separations Module
Press Menu/
Status for monitoring and direct control
User:
Revision 2.04
Develop
Methods Run
Samples Diag Config Log
01/02/2007 09:00:00 pm
© 2001 Waters Corporation
Tester
Waters 2795
**
Firmware
version
2 Setting the Operating Mode to Remote Control
6Waters 2795 Separations Module Getting Connected Guide Thermo Scientific
2. Press the CONFIG key (Figure 4) to open the Configuration page (Figure 5).
Figure 4. Front panel LCD display and keypad for the Waters 2795 Separations Module
Figure 5. Configuration page
?
123
456
789
0.
Exit Menu/
Status
Stop
Flow
Enter
Clear
Display
Contrast
Diag Config Log
Main
<Default> Idle
Separations Module
Press Menu/
Status for monitoring and direct control
User:
Revision 2.04
Develop
Methods Run
Samples Diag Config Log
01/02/2007 09:00:00 pm
© 2001 Waters Corporation
Tester
Waters 2795
**
Arrow keys
Config key
Enter key
Exit key
Configuration
<Default> Idle
Tester
Pressure Units:
500
Syringe Size: Loop Size:
System
Remote Control via RS-232
Printer Date Format: Alarm Sound:
User
Names
Detec-
tors Options
Set
Clock
Events
In
More
psi
MDY
Baud Rate:
1200
None Continuous
50
µL µL
IEEE 488 Address:
10
o
2 Setting the Operating Mode to Remote Control
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 7
3. Highlight the System box by pressing the RIGHT ARROW ( ) key, and then press
ENTER on the front panel keypad (Figure 4) to view the list of system options
(Figure 6).
Figure 6. Configuration page with the list of system options
4. Select Controlled via RS232 by pressing the UP ARROW ( ) and DOWN ARROW
( ) keys, and then press ENTER to save the change.
5. Press EXIT to return to the Main page.
Configuration
<Default> Idle
Tester
Pressure Units:
500
Syringe Size: Loop Size:
System
Remote Control via RS-232
Printer Date Format: Alarm Sound:
User
Names
Detec-
tors Options
Set
Clock
Events
In
More
psi
MDY
Baud Rate:
1200
None Continuous
50
µL µL
IEEE 488 Address:
10
o
No Interaction
System Controller
Controlled by Millennium 32
Controlled by MassLynx
Controlled by Millennium 2.XX
Operate gradient by event in
Controlled via RS232
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 9
3
Adding the Waters 2795 Separations Module to the
Software Configuration
This chapter describes how to add the Waters 2795 Separations Module to the Xcalibur
instrument configuration, including how to specify the port on the data system computer
where you connected the serial communication cable and the sample plates you are using.
To add the Waters 2795 Separations Module to the Xcalibur instrument configuration
1. From the Microsoft Windows XP taskbar, choose Start > Programs >
Thermo Foundation 1.0 > Instrument Configuration to open the Instrument
Configuration window (Figure 7).
Figure 7. Xcalibur Instrument Configuration window
3 Adding the Waters 2795 Separations Module to the Software Configuration
10 Waters 2795 Separations Module Getting Connected Guide Thermo Scientific
2. Under Available Devices, double-click the Waters 2795 icon to add it to the Configured
Devices list.
3. Under Configured Devices, double-click the Waters 2795 icon to open the
Configuration dialog box (Figure 8).
Figure 8. Alliance 2795 Configuration dialog box
4. Under RS-232 Configuration, in the Port list, confirm that the selected COM port is the
RS232 port on the data system computer where you physically connected the separations
module.
For information about connecting the serial communication cable, see “Connecting the
Serial Communication Cable” on page 4.
IMPORTANT When the correct COM port is not selected in the Port list, the
Xcalibur data system does not communicate with the Waters 2795 Separations
Module.
3 Adding the Waters 2795 Separations Module to the Software Configuration
Thermo Scientific Waters 2795 Separations Module Getting Connected Guide 11
5. In the Carrier Plate lists, specify the Plate Type that you are using and the Sequential
Reference counting order for each plate.
6. Click Autoconfigure, and then click OK to save the settings and close the dialog box.
The Waters 2795 Separations Module is ready for remote control from the Xcalibur data
system.
IMPORTANT The Waters 2795 Separations Module does not automatically
recognize the tray type loaded in the sample compartment. You must specify the tray
type to ensure that the autosampler draws sample from the correct location.
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Thermo Fisher Scientific Waters 2795 Separations Module User guide

Brand
Thermo Fisher Scientific
Model
Waters 2795 Separations Module
Type
User guide
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