Thermo Fisher Scientific CpG Methylated Jurkat Genomic DNA User guide
- Type
- User guide
PRODUCT INFORMATION
CpG Methylated
Jurkat Genomic DNA
#SD1121 15 µg
Lot: ___
Concentration: 0.1 mg/mL
Store at -20°C
h
www.thermoscientific.com/onebio
Description
Human male genomic DNA from Jurkat cells, enzymatically
methylated with CpG Methyltransferase (M.SssI).
Genomic DNA was isolated from Jurkat E6.1 (human acute
T-cell leukemia, clone E6.1) cells grown to confluency in
RPMI media supplemented with 10% bovine fetal serum.
Isolated genomic DNA was treated with CpG
Methyltransferase, repurified and dissolved in TE buffer.
≥98% CpG methylation level confirmed by bisulfite
sequencing.
Applications
As controls in the following:
• Bisulfite sequencing;
• Combined Bisulfite Restriction Analysis (COBRA);
• Methylation-sensitive Single-Nucleotide Primer Extension
(Ms-SNuPE).
Storage Buffer
DNA is supplied in TE buffer (10 mM Tris-HCl pH 8.0,
1 mM EDTA).
Rev.2
CERTIFICATE OF ANALYSIS
Predominant >30 kb band is observed during agarose gel
electrophoresis. DNA concentration is determined
spectrophotometrically. The absence of nucleases is
confirmed by labeled oligonucleotide tests.
CpG methylated DNA was protected from digestion by the
methylation sensitive restriction enzyme HpaII as
determined by qPCR analysis. Following qPCR, equivalent
Cq values for HpaII digested and control undigested DNA
are observed.
DNA purity is confirmed through a functional qPCR test in
which Cq values of MspI and HpaII digested template DNA
must differ by ≥5 cycles.
A260/280 ratio 1.75-1.98
A260/230 ratio ≥2
The absence of mycoplasma DNA is confirmed by PCR.
Quality authorized by:
Jurgita Zilinskiene
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and
in vitro use only. The product was not tested for use in diagnostics or for drug
development, nor is it suitable for administration to humans or animals.
Please refer to www.thermoscientific.com/onebio for Material Safety Data Sheet of
the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
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Thermo Fisher Scientific CpG Methylated Jurkat Genomic DNA User guide
- Type
- User guide
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