Thermo Fisher Scientific Jurkat Genomic DNA User guide

Brand
Thermo Fisher Scientific
Model
Jurkat Genomic DNA
Type
User guide
PRODUCT INFORMATION
Jurkat Genomic DNA
#SD1111 15 µg
Lot: ___
Concentration: 0.1 mg/mL
Store at -20°C
V
www.thermoscientific.com/onebio
Description
Human male genomic DNA from Jurkat cells.
Genomic DNA was isolated from Jurkat E6.1 (human acute
T-cell leukemia, clone E6.1) cells grown to confluency in
RPMI media supplemented with 10% bovine fetal serum.
Isolated genomic DNA is dissolved in TE buffer.
Applications
• As controls in the following:
− Bisulfite sequencing;
− Combined Bisulfite Restriction Analysis (COBRA);
− Methylation-sensitive Single-Nucleotide Primer
Extension (Ms-SNuPE).
• Genomic DNA library construction.
• PCR, Southern blotting.
Storage Buffer
DNA is supplied in TE buffer (10 mM Tris-HCl pH 8.0,
1 mM EDTA).
Rev.2
CERTIFICATE OF ANALYSIS
Predominant >30 kb band is observed during agarose gel
electrophoresis.
DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by labeled
oligonucleotide tests.
A260/280 ratio 1.75-1.98
A260/230 ratio ≥2
The absence of mycoplasma DNA is confirmed by PCR.
Quality authorized by:
Jurgita Zilinskiene
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and
in vitro use only. The product was not tested for use in diagnostics or for drug
development, nor is it suitable for administration to humans or animals.
Please refer to www.thermoscientific.com/onebio for Material Safety Data Sheet of
the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
  • Page 1 1
  • Page 2 2

Thermo Fisher Scientific Jurkat Genomic DNA User guide

Brand
Thermo Fisher Scientific
Model
Jurkat Genomic DNA
Type
User guide
Download PDF
report

Ask a question and I''ll find the answer in the document

Finding information in a document is now easier with AI

Related papers