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Thermo Fisher Scientific Jurkat Genomic DNA User guide

  • Hello! I've reviewed the product details for the Thermo Scientific Jurkat Genomic DNA SD1111. This document outlines the key features, applications including its use in Bisulfite sequencing and PCR, and storage information of the product. I'm ready to answer your questions about its contents or other aspects of the DNA!
  • What is the source of this genomic DNA?
    What buffer is the DNA supplied in?
    What are some of the applications for this genomic DNA?
PRODUCT INFORMATION
Jurkat Genomic DNA
#SD1111 15 µg
Lot: ___
Concentration: 0.1 mg/mL
Store at -20°C
V
www.thermoscientific.com/onebio
Description
Human male genomic DNA from Jurkat cells.
Genomic DNA was isolated from Jurkat E6.1 (human acute
T-cell leukemia, clone E6.1) cells grown to confluency in
RPMI media supplemented with 10% bovine fetal serum.
Isolated genomic DNA is dissolved in TE buffer.
Applications
• As controls in the following:
− Bisulfite sequencing;
− Combined Bisulfite Restriction Analysis (COBRA);
− Methylation-sensitive Single-Nucleotide Primer
Extension (Ms-SNuPE).
• Genomic DNA library construction.
• PCR, Southern blotting.
Storage Buffer
DNA is supplied in TE buffer (10 mM Tris-HCl pH 8.0,
1 mM EDTA).
Rev.2
CERTIFICATE OF ANALYSIS
Predominant >30 kb band is observed during agarose gel
electrophoresis.
DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by labeled
oligonucleotide tests.
A260/280 ratio 1.75-1.98
A260/230 ratio ≥2
The absence of mycoplasma DNA is confirmed by PCR.
Quality authorized by:
Jurgita Zilinskiene
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and
in vitro use only. The product was not tested for use in diagnostics or for drug
development, nor is it suitable for administration to humans or animals.
Please refer to www.thermoscientific.com/onebio for Material Safety Data Sheet of
the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the
property of Thermo Fisher Scientific Inc. and its subsidiaries.
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