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  3. pUC18, pUC19 DNA

Thermo Fisher Scientific pUC18, pUC19 DNA User guide

  • Hello! I've reviewed the product detail for the Thermo Scientific pUC19 DNA plasmid, specifically model #SD0061. This document outlines the plasmid's characteristics, such as its size, molecular weight, and applications like cloning and sequencing. I'm ready to answer any questions you have about this product.
  • What is the size of the plasmid?
    What is the molecular weight?
    What is the storage buffer?
    What is it used for?
PRODUCT INFORMATION
pUC19 DNA
#SD0061 50 µg
Lot: _
Concentration: 0.5 µg/µL
Store at -20°C
www.thermoscientific.com/onebio
Description
Double-stranded closed circular high copy plasmid, 2686
base pairs with a molecular weight of 1.74 × 10
6
Da. Isolated
from E.coli (dam
+
, dcm
+
).
GeneBank/EMBL accession number L09137. For map and
features see www.thermoscientific.com/reviewer.
Features
• Purified by chromatography using proprietary patented
technology.
• More than 90% in the supercoiled form.
Applications
• Cloning.
• Sequencing of insert DNA.
Storage Buffer
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
CERTIFICATE OF ANALYSIS
DNA concentration is confirmed spectrophotometrically.
Identity and homogeneity of DNA is confirmed by digestion
by NdeI+HindIII and SmaI and analysis of DNA fragmentation
patterns.
Quality authorized by: Jurgita Zilinskiene
PRODUCT USE LIMITATION
This product is developed, designed and sold exclusively for research purposes and in vitro use only.
The product was not tested for use in diagnostics or for drug development, nor is it suitable for
administration to humans or animals. Please refer to www.thermoscientific.com/onebio for Material
Safety Data Sheet of the product.
© 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo
Fisher Scientific Inc. and its subsidiaries.
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