Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

Brand
Thermo Fisher Scientific
Model
Axiom 2.0 Assay 96-Array Format
Type
Owner's manual
For Research Use Only. Not for use in diagnostic procedures.
Axiom™ 2.0 Assay 96-Array Format Manual
Workflow
SITE PREPARATION GUIDE
for use with:
Axiom™ Arrays
Axiom™ 2.0 Reagent Kit
Catalog Number 901758
Publication Number MAN0018132
Revision C.0
Thermo Fisher Scientific Baltics UAB |
V.A. Graiciuno 8, LT-02241 |
Vilnius, Lithuania
Products manufactured at this site:
Axiom™ 2.0 Reagent Kit
Aymetrix Pte Ltd |
7 Gul Circle #2M-01 |
Keppel Logistics Building |
Singapore 629563
Products manufactured at this site:
•Axiom™ 96F Array Plates
•Axiom™ myDesign™ Array Plates
Revision history:MAN0018132 C.0 (English)
Revision Date Description
C.0 5 October 2023 Adding GeneTitan™ MC Fast Scan Instrument information.
B.0 19 May 2022 Add the following product options:
•MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with Barcode
•Bio-Rad™ HSP9631, HSP9601, HSS9601, and HSS9641 PCR plates.
•Eppendorf™ DeepWell™ Plate 96 for microbiome assay use.
•ProFlex™ 96‑well PCR System thermal cycler.
•Genomic DNA Standard (Ref 103) positive control, available for purchase separately.
•Axiom™ 2.0 Assay Training Module for CEU Arrays
A.0 26 December
2018 •Initial release in Thermo Fisher Scientific document control system.
•Supersedes legacy Aymetrix publication number 702991.
•Updated to the current document template, with associated updates to trademarks, logos, licensing, and
warranty.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Microsoft,
and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries. Vortex-
Genie is a registered trademark of Scientific Industries, Inc. Jitterbug is a trademark of Boekel Scientific. Eppendorf is a registered
trademark of Eppendorf AG. Molecular Devices and SpectraMax are trademarks of Molecular Devices Corporation. Pipet-Lite is a
trademark of Rainin Instrument, LLC. Pipet-Aid is a registered trademark of Drummond Scientific Company. Bio-Rad, Microseal, DNA
Engine Tetrad, DNA Engine, and Hard-Shell are registered trademarks of Bio-Rad Laboratories, Inc.
©2018-2023 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format
ManualWorkflow ................................................................... 4
Equipment, software, reagents, and arrays required ................................. 4
Equipment required for manual target preparation .................................. 6
Instruments required ........................................................ 6
Preamplification area/amplification staging area ................................ 7
Oven requirements .......................................................... 7
Spectrophotometers ........................................................ 8
Thermal cycler recommendations ............................................. 8
Thermal cyclerconsumables ................................................ 10
Platecentrifuge ............................................................ 10
Sample plates ............................................................. 12
Pipettes andtips .......................................................... 12
Other labware required ..................................................... 13
Other equipment, reagents, and gels required ................................. 14
Other lab equipment required ............................................... 14
■Documentation and support ....................................................... 16
Relateddocumentation ......................................................... 16
Customer and technical support ................................................. 17
Limited product warranty ........................................................ 17
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 3
Equipment and supplies for the
Axiom™ 2.0 Assay 96-Array Format
Manual Workflow
This site preparation guide includes the supplier and catalog number information for the equipment,
software, reagents, arrays, labware, and other consumables that have been verified for use with the
Axiom™ 2.0 Assay 96-Array Format Manual Workflow.
Equipment, software, reagents, and arrays required
Unless otherwise indicated, all materials are available through thermofisher.com.
Item Source
Equipment
GeneTitan™ Multi-Channel (MC) Instrument[1], or
GeneTitan™ MC Fast Scan Instrument
00-0373
00-0370
Software
GeneChip™ Command Console™version 6.1 or later
Axiom™ Analysis Suite version 5.1.1 or
later
Reagents
Axiom™ 2.0 Reagent Kit (96 reactions) 901758
Genomic DNA Standard (Ref 103) 951957
Arrays
Axiom™ 96-array format array plates
Visit our website for a complete list of array plates available for use with the Axiom™ 2.0
Assay.
Varies, depending
on array design
Axiom™ Microbiome Array Plate, 96-array format 902904
4Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
(continued)
Item Source
Consumables
Axiom™ GeneTitan™ Consumables Kit
Each kit Includes:
•One 96-layout hybridization tray
•One 96-layout scan tray with cover and protective base
•Five 96-layout stain trays with covers
These trays are required for processing Axiom™ array plates on the GeneTitan™ MC
Instrument.
Note: Consumables are included with the Axiom™ Genome-Wide BOS and Pan-African
Genotyping Bundles.
901606
Training modules
Axiom™ 2.0 Assay Training Module with CEU Arrays
Kit contains:
•2 Axiom™ Genome-Wide CEU 1 Array Plates
•2 full plates of 96 HapMap DNA samples in deep well titer plate[2]
•2 Axiom™ 2.0 Reagent Kits (96 reactions)
•2 Axiom™ GeneTitan™ Consumables Kits
•1 GeneTitan™ ZeroStat AntiStatic Gun
901767
Axiom™ 2.0 Assay Training Module with ASI Arrays
Kit contains:
•2 Axiom™ Genome-Wide ASI 1 Array Plates
•2 full plates of 96 HapMap DNA samples in deep well titer plate[2]
•2 Axiom™ 2.0 Reagent Kits (96 reactions)
•2 Axiom™ GeneTitan™ Consumables Kits
•1 GeneTitan™ ZeroStat AntiStatic Gun
901768
Axiom™ 2.0 Assay Training Module with CEU and BOS Arrays
Kit contains:
•1 Axiom™ Genome-Wide BOS 1 Array Plate
•1 Axiom™ Genome-Wide CEU 1 Array Plate
•1 full plate of 96 HapMap DNA samples in deep well titer plate[2]
•2 Axiom™ 2.0 Reagent Kits (96 reactions)
•2 Axiom™ GeneTitan™ Consumables Kits
•1 GeneTitan™ ZeroStat AntiStatic Gun
901750
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment, software, reagents, and arrays required
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 5
(continued)
Item Source
Axiom™ 2.0 Pan-African Training Module
Kit contains:
•1 Axiom™ Genome-Wide Pan-African Genotyping Bundle (1 set of 96-array plates,
3 Axiom™ 2.0 Reagent Kits, and 3 Axiom™ GeneTitan™ Consumables Kits)
•3 identical 96 HapMap DNA plates[2]
•1 GeneTitan™ ZeroStat AntiStatic Gun
901769
[1] For a complete list of all equipment and supplies required for GeneTitan™ MC Instrument installation and operation, see the
GeneTitan™ Multi-Channel Instrument Site Preparation Guide.
[2] NOTE: Per agreement with Coriell Life Sciences, all residual HapMap DNA samples must be destroyed immediately following
completion of training.
Equipment required for manual target preparation
Instruments required
The following instruments are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow.
Unless otherwise indicated, all materials are available through thermofisher.com.
Equipment Source
Two or 3 ovens See “Oven requirements” on page7.
Fume hood (Strongly recommended.) See the Axiom™ 2.0 Assay 96-Array Format
Manual Workflow User Guide.
One of the following thermal cyclers:
•Applied Biosystems™ ProFlex™ PCR System
•Applied Biosystems™ GeneAmp™ PCR System 9700
•Applied Biosystems™ 2720 Thermal Cycler
•Bio-Rad™ DNA Engine™ PTC-200 Thermal Cycler/MJ
PTC-200
•Bio-Rad™ DNA Engine Tetrad™ 2 PTC-0240G
See “Thermal cycler recommendations” on page8.
•Thermo Fisher Scientific
•Thermo Fisher Scientific
•Thermo Fisher Scientific
•Bio-Rad Laboratories™
•Bio-Rad Laboratories™
One of the following shakers:
•Thermo Scientific™ Digital Microplate Shaker
•Thermo Scientific™ Compact Digital Microplate Shaker
•Jitterbug™ shaker
 
•88882005 or 88882006
•88880023
•Boekel™ Scientific, 130000
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
6Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
Preamplification area/amplification staging area
Precautions are required when manipulating genomic DNA or setting up amplification reactions to avoid
contamination with foreign DNA amplified in other reactions and procedures. It is recommended that
genomic DNA manipulations and amplification reaction set up are performed in a dedicated room or
area separate from the main laboratory.
These areas should have dedicated sets of pipettes and plasticware. If no dedicated area is available,
use of a dedicated bench or a dedicated biosafety hood and dedicated pipettes is suggested. If no
dedicated bench or biosafety hood is available, a set of dedicated pipettes is recommended.
Ideally, the preamplification and amplification staging areas would be separate; however these areas
may be combined due to space and equipment limitations.
Oven requirements
Multiple ovens are required for manual target preparation. The exact number depends upon whether
you are running only a single sample plate and array plate through the workflow, or whether you are
trying to run the 3 plate/week manual target preparation protocol.
•If you are running individual plates, you will need 2 ovens for the workflow.
•If you are running the 3 plate/week workflow, a third oven is highly recommended.
See chapter Processing Three Axiom™ Array Plates per Week with Manual Target Preparation in the
Axiom™ 2.0 Assay 96-Array Format Manual Workflow User Guide for more information.
Table1Suggested settings for ovens when performing the 3 plate/week manual target preparation
protocol.
Day of workflow Oven 1 Oven 2 Oven 3
Day 1 37°C N/A N/A
Day 2 37°C 65°C 37°C
Day 3 48°C 65°C 37°C
Day 4 48°C 65°C 37°C
Day 5 N/A N/A N/A
Two to 3 ovens are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow. Use any
combination of the following ovens.
Table2Oven recommendations.
Equipment Source
Thermo Scientific™ Heratherm™ Advanced Protocol Microbiological Incubator,
capacity 66 L
•120V, 60 Hz
•230V, 50/60 Hz
 
•51028066
•51028133
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 7
Table 2 Oven recommendations.(continued)
Equipment Source
BINDER™ ED 56 Drying and Heating Chamber
•ED056UL-120V Voltage: 120 V 1~60 Hz
•ED056-230V Voltage: 230 V 1~50/60 Hz
 
•BINDER™, 9010-0334
•BINDER™, 9010-0333
BINDER™ BD 56 Standard-Incubator with natural convection
•BD056UL-120V Voltage: 120 V 1~60 Hz
•BD056-230V Voltage: 230 V 1~50/60 Hz
 
•BINDER™, 9010-0324
•BINDER™, 9010-0323
GeneChip™ Hybridization Oven 645 00-0331
Spectrophotometers
We recommend that you use one of the following spectrophotometers, or the equivalent.
Unless otherwise indicated, all materials are available through thermofisher.com.
Item Source
Thermo Scientific™ Multiskan™ Sky Microplate Spectrophotometer 51119500
DTX 880 Multimode Detector with genomic filter slide Beckman Coulter™
987921 Detector
A30184 Filter slide
Thermal cycler recommendations
We have verified the performance of this assay using the following thermal cyclers:
•Applied Biosystems™ ProFlex™ 96‑well PCR System
•Applied Biosystems™ ProFlex™ 2×96‑well PCR System
•Applied Biosystems™ GeneAmp™ PCR System 9700 (with gold-plated or silver block)
•Applied Biosystems™ 2720 Thermal Cycler
•Bio-Rad™ DNA Engine™ PTC-200 Thermal Cycler/MJ PTC-200
•Bio-Rad™ DNA Engine Tetrad™ 2 PTC-0240G
The performance of this assay has not been verified with other thermal cyclers. Use of other thermal
cyclers may result in assay failure and may violate the Axiom™ array and reagent replacement policy.
The thermal cycler needs to be programmed with the Axiom 2.0 Denature protocol:
95°C
48°C 48°C
10 min
3 min Infinity
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
8Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
Use the heated lid option when setting up or running the protocol.
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 9
Thermal cycler consumables
The following table provides details about the consumables to be used with each thermal cycler.
Unless otherwise indicated, all materials are available through thermofisher.com.
Thermal cycler model PCR plate type Seal
Applied Biosystems™ ProFlex™
PCR System
Bio-Rad™Hard-Shell™ 96-Well PCR Plate,
high profile, semi skirted, HSS9601 or
HSS9641.
MicroAmp™ Clear Adhesive
Film (Cat. No. 4306311)
Applied Biosystems™
GeneAmp™ PCR System 9700
(with gold-plated or silver
block)
Bio-Rad™Hard-Shell™ 96-Well PCR Plate,
high profile, semi skirted, HSS9601 or
HSS9641.
MicroAmp™ Clear Adhesive
Film (Cat. No. 4306311)
Applied Biosystems™ 2720
Thermal Cycler
Bio-Rad™Hard-Shell™ 96-Well PCR Plate,
high profile, semi skirted, HSS9601 or
HSS9641.
MicroAmp™ Clear Adhesive
Film (Cat. No. 4306311)
Bio-Rad™ DNA Engine™
PTC-200 Thermal Cycler
Bio-Rad™ Hard-Shell™ 96-Well PCR Plate,
low profile, full skirted, HSP9631 (blue) or
HSP9601 (white).
MicroAmp™ Clear Adhesive
Film (Cat. No. 4306311)
Bio-Rad™ DNA Engine Tetrad™
2
Bio-Rad™ Hard-Shell™ 96-Well PCR Plate,
low profile, full skirted, HSP9631 (blue) or
HSP9601 (white).
MicroAmp™ Clear Adhesive
Film (Cat. No. 4306311)
Note: Bio-Rad™ Microseal™ 'B' PCR Plate Sealing Film (Bio-Rad™, MSB1001) may be used in place of
MicroAmp™ Clear Adhesive Film for the Bio-Rad™ and Applied Biosystems™ thermal cyclers.
For Axiom™ Microbiome applications, use only the MicroAmp™ Clear Adhesive Film. The Microseal™ 'B'
PCR Plate Sealing Film has not been validated for use with Axiom™ Microbiome applications.
Plate centrifuge
One plate centrifuge is required for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow. We
recommend the plate centrifuges listed in the following table. When centrifuging and drying pellets, the
centrifuge must be able to centrifuge plates at:
•Rcf: 3,200x g (4,000rpm for the Eppendorf™ Centrifuge 5810 R) with an appropriate rotor/bucket
combination
•Temperature: 4°C
In addition, the bottom of the rotor buckets should be soft rubber to ensure that the deepwell plates do
not crack. Do not use buckets where the plates sit directly on a metal or hard plastic bottom.
For the Eppendorf™ Centrifuge 5810 R, do not use the A-4-62 rotor with a WO-15 plate carrier (hard
bottom).
Unless otherwise indicated, all materials are available through thermofisher.com.
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
10 Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
Table3Plate centrifuges recommended for the Axiom™ 2.0 Assay 96-Array Format Manual
Workflow.
Item Source
Sorvall™ Legend™ XTR Centrifuge
(refrigerated), with:
•TX-750 4 + 750mL Swinging Bucket Rotor
•Carrier for microplates (including plate
trays and neoprene pads)
75004521 (60 Hz, 120 V, floor model)
75004523 (50–60 Hz, 230 V, USA and Canada, floor model)
75004520 (50 Hz, 230 V, floor model)
75003017 (bucket)
One of either:
•75003795 (set of 2 carriers)
•75003617 (set of 4 carriers)
Allegra™ 25R Benchtop Centrifuge,
Refrigerated
Beckman Coulter™
369434 (60Hz, 280V)
369435 (50/60Hz, 200V)
369436 (50Hz, 230V)
368954 S5700 Swinging-Bucket Rotor
Centrifuge 5810 R (refrigerated)
A-4-81 MTP/Flex swinging bucket rotor with 4
microtest-plate buckets
Eppendorf™
022625501 (60Hz, 120V)
022625101 (50 Hz, 120 V, 20 AMP version)
022625551 (50Hz, 230V)
022638807
Relative centrifugal force (rcf) can be calculated as follows:
rcf = (1.118 × 10-5) R S2
where
•R is the radius of the rotor in centimeters
•S is the speed of the centrifuge in revolutions per minute
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 11
Sample plates
The following sample plates are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow.
Unless otherwise indicated, all materials are available through thermofisher.com.
Equipment Source
Abgene™ 96 Well 2.2 mL Polypropylene Deepwell Storage Plate Mark II,
2.2 mL. (For use with Axiom™ Genotyping Assay.)
AB0932
Eppendorf™ 96 Deepwell Plate, 2,000 µL (For use with Axiom™
Microbiome Assay.)
Note: This is the only plate supported for Axiom™ microbiome use.
Using a dierent plate type may result in assay failure.
Eppendorf™, 951033481
Bio-Rad™ Hard-Shell™ 96-Well PCR Plate, low profile, full skirted Bio-Rad Laboratories™, HSP9631
(blue) or HSP9601 (white)
OD Plate, option 1:
Corning™ UV-Transparent Microplate
Axiom™ 96-format Consumables
Kit for QC, 902909.
Also available from Fisher
Scientific™, 07-200-623
OD Plate, option 2:
Greiner Bio-One™ UV-Star™ 96-Well UV Spectroscopy Microplate
Fisher Scientific™, 07-000-407
Bio-Rad™Hard-Shell™ 96-Well PCR Plate, high profile, semi skirted
Required only if using Applied Biosystems™ 9700 or 2720 or ProFlex™
PCR System thermal cyclers.
Bio-Rad Laboratories™, HSS9601
(clear) or HSS9641 (green)
MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with
Barcode
4483354 or 4483352
Pipettes and tips
Pipettes and tips recommended for manual target preparation are listed in the following table.
Equipment Rainin™ part number
Pipet-Lite™, magnetic-assist, single-channel P20 L-20
Pipet-Lite™, magnetic-assist, single-channel P200 L-200
Pipet-Lite™, magnetic-assist, single-channel P1000 L-1000
Pipette, 12-channel P20 L12-20
Pipette, 12-channel P50 (optional) L12-50
Pipette, 12-channel P200 L12-200
Pipette, 12-channel P1200 L12-1200
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
12 Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
(continued)
Equipment Rainin™ part number
Pipette tips GP = refill GP-L10F
Pipette tips GP = refill GP-L200F
Pipette tips GP = refill GP-L1000F
Pipette tips RT = with rack RT-L10F
Pipette tips RT = with rack RT-L200F
Pipette tips RT = with rack RT-L1000F
Other labware required
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Conical tube, 50mL VWR™, 21008-178
Conical tube, 15mL VWR™, 21008-202
Tube Holder, 15 mL and 50mL MLS
Reagent reservoir, 100 mL sterile, multichannel VWR™, 89092-836
25-mL Matrix™ Reagent Reservoir Fisher Scientific™, 809311
96 well plate metal chamber Diversified Biotech™,
CHAM-1000
10-mL serological pipettes VWR™, 89130-898 or MLS
5-mL serological pipettes VWR™, 89130-896 or MLS
MicroAmp™ Clear Adhesive Film 4306311
Bio-Rad™ Microseal™ 'B' PCR Plate Sealing Film
Note: For Axiom™ Microbiome applications, use only the MicroAmp™
Clear Adhesive Film. The Microseal™ 'B' PCR Plate Sealing Film has not
been validated for use with Axiom™ Microbiome applications.
Bio-Rad™, MSB1001
Laboratory wipes MLS
1.7-mL microcentrifuge tubes, DNAse and RNAse-free MLS
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 13
Other equipment, reagents, and gels required
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Table4Other reagents required.
Item Source
2-Propanol, anhydrous, 99.5% (Isopropanol) Sigma-Aldrich™, 278475
TE Buer, 1X Solution pH 8.0, Low EDTA J75793.AP
Table5Equipment, reagents, and gels required to run QC steps.
Item Source
E‑Gel™ Power Snap Plus Electrophoresis Device G9110
Safe Imager™ Viewing Glasses S37103
E‑Gel™ Power Snap Plus Camera G9200
Invitrogen™ E‑Gel™ 48 Agarose Gels, 4% (for Axiom™ QC) G800804
Invitrogen™ E‑Gel™ 48 Agarose Gel, 1% (for gDNA QC) G800801
Invitrogen™ E‑Gel™ 96 High Range DNA Marker (for gDNA QC) 12352019
Invitrogen™ RediLoad™ Loading Buer (for gDNA QC) 750026
Applied Biosystems™25bp DNA Ladder or equivalent (for Axiom™ QC) 931343
Invitrogen™ TrackIt™ Ultra Low Range DNA Ladder 10488023
Invitrogen™ TrackIt™ Cyan/Orange Loading Buer (for Axiom™ QC) 10482028
UltraPure™ DNase/RNase-Free Distilled Water 10977023
Other lab equipment required
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Bel-Art™ Cryo-Safe™ Mini Cooler, –15℃[1] Fisher Scientific™, 03-410-497
Fine-point permanent markers MLS
Freezer, –20°C MLS
Ice bucket, 4—9 liters MLS
Mini Microcentrifuge for 2mL tubes MLS
Electronic pipettor for serological pipettes MLS
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
14 Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
(continued)
Item Source
Refrigerator, 2—8°C MLS
Vortexer (for plates and microtubes) MLS
[1] Equivalent items from other manufacturers are acceptable.
Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workflow
Equipment required for manual target preparation
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 15
Documentation and support
Related documentation
Document Publication number
Axiom™ 2.0 Assay 96-Array Format Manual Workflow User Guide MAN0018000
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Quick Reference MAN0018119
Axiom™ Microbiome Solution User Guide MAN0019888
Axiom™ 2.0 gDNA Sample Preparation Quick Reference MAN0017720
Axiom™ gDNA Sample Prep for Genome-Wide BOS 1 Array Plate Quick Reference 702975
Axiom™ Genotyping Solution Data Analysis User Guide MAN0018363
GeneChip™ Command Console™ (GCC) User Guide MAN0027771
Axiom™ Analysis Suite User Guide MAN0027928
GeneTitan™ Multi-Channel Instrument User Guide MAN0027694
GeneTitan™ Multi-Channel Instrument Site Preparation Guide MAN0025571
GeneTitan™ MC Protocol for Axiom™ Array Plate Processing Quick Reference MAN0017718
Recommended Alternative Microarray Consumables Quick Reference MAN0019853
16 Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide
Customer and technical support
Visit thermofisher.com/support for the latest service and support information.
•Worldwide contact telephone numbers
•Product support information
–Product FAQs
–Software, patches, and updates
–Training for many applications and instruments
•Order and web support
•Product documentation
–User guides, manuals, and protocols
–Certificates of Analysis
–Safety Data Sheets (SDSs; also known as MSDSs)
Note: For SDSs for reagents and chemicals from other manufacturers, contact the
manufacturer.
Limited product warranty
Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the
Life Technologies' General Terms and Conditions of Sale at www.thermofisher.com/us/en/home/
global/terms-and-conditions.html. If you have any questions, please contact Life Technologies at
www.thermofisher.com/support.
Documentation and support
Customer and technical support
Axiom™ 2.0 Assay 96-Array Format Manual Workflow Site Preparation Guide 17
Axiom 2.0 Assay 96F Manual Workflow_SPG_MAN0018132-v4-GUID-
C8A9BF67-5EF3-4401-81EB-99069EE255DE-2023/10/05 22:46:29 en
22:51:24.779+01:00
thermofisher.com/support|thermofisher.com/askaquestion
thermofisher.com
5 October 2023
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Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

Brand
Thermo Fisher Scientific
Model
Axiom 2.0 Assay 96-Array Format
Type
Owner's manual
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