Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

Brand: Thermo Fisher Scientific

Model: Axiom 2.0 Assay 96-Array Format

Type: Owner's manual

For Research Use Only. Not for use in diagnostic procedures.

Axiom™ 2.0 Assay 96-Array Format Manual

Workﬂow

SITE PREPARATION GUIDE

for use with:

Axiom™ Arrays

Axiom™ 2.0 Reagent Kit

Catalog Number 901758

Publication Number MAN0018132

Revision C.0

Thermo Fisher Scientiﬁc Baltics UAB |

V.A. Graiciuno 8, LT-02241 |

Vilnius, Lithuania

Products manufactured at this site:

Axiom™ 2.0 Reagent Kit

Aymetrix Pte Ltd |

7 Gul Circle #2M-01 |

Keppel Logistics Building |

Singapore 629563

Products manufactured at this site:

•Axiom™ 96F Array Plates

•Axiom™ myDesign™ Array Plates

Revision history:MAN0018132 C.0 (English)

Revision Date Description

C.0 5 October 2023 Adding GeneTitan™ MC Fast Scan Instrument information.

B.0 19 May 2022 Add the following product options:

•MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with Barcode

•Bio-Rad™ HSP9631, HSP9601, HSS9601, and HSS9641 PCR plates.

•Eppendorf™ DeepWell™ Plate 96 for microbiome assay use.

•ProFlex™ 96‑well PCR System thermal cycler.

•Genomic DNA Standard (Ref 103) positive control, available for purchase separately.

•Axiom™ 2.0 Assay Training Module for CEU Arrays

A.0 26 December

2018 •Initial release in Thermo Fisher Scientiﬁc document control system.

•Supersedes legacy Aymetrix publication number 702991.

•Updated to the current document template, with associated updates to trademarks, logos, licensing, and

warranty.

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE

LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR

ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these

products, you accept the terms and conditions of all applicable Limited Use Label Licenses.

TRADEMARKS: All trademarks are the property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed. Microsoft,

and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries. Vortex-

Genie is a registered trademark of Scientiﬁc Industries, Inc. Jitterbug is a trademark of Boekel Scientiﬁc. Eppendorf is a registered

trademark of Eppendorf AG. Molecular Devices and SpectraMax are trademarks of Molecular Devices Corporation. Pipet-Lite is a

trademark of Rainin Instrument, LLC. Pipet-Aid is a registered trademark of Drummond Scientiﬁc Company. Bio-Rad, Microseal, DNA

Engine Tetrad, DNA Engine, and Hard-Shell are registered trademarks of Bio-Rad Laboratories, Inc.

Contents

■Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format

ManualWorkﬂow ................................................................... 4

Equipment, software, reagents, and arrays required ................................. 4

Equipment required for manual target preparation .................................. 6

Instruments required ........................................................ 6

Preampliﬁcation area/ampliﬁcation staging area ................................ 7

Oven requirements .......................................................... 7

Spectrophotometers ........................................................ 8

Thermal cycler recommendations ............................................. 8

Thermal cyclerconsumables ................................................ 10

Platecentrifuge ............................................................ 10

Sample plates ............................................................. 12

Pipettes andtips .......................................................... 12

Other labware required ..................................................... 13

Other equipment, reagents, and gels required ................................. 14

Other lab equipment required ............................................... 14

■Documentation and support ....................................................... 16

Relateddocumentation ......................................................... 16

Customer and technical support ................................................. 17

Limited product warranty ........................................................ 17

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 3

Equipment and supplies for the

Axiom™ 2.0 Assay 96-Array Format

Manual Workﬂow

This site preparation guide includes the supplier and catalog number information for the equipment,

software, reagents, arrays, labware, and other consumables that have been veriﬁed for use with the

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow.

Equipment, software, reagents, and arrays required

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Item Source

Equipment

GeneTitan™ Multi-Channel (MC) Instrument[1], or

GeneTitan™ MC Fast Scan Instrument

00-0373

00-0370

Software

GeneChip™ Command Console™version 6.1 or later

Axiom™ Analysis Suite version 5.1.1 or

later

Reagents

Axiom™ 2.0 Reagent Kit (96 reactions) 901758

Genomic DNA Standard (Ref 103) 951957

Arrays

Axiom™ 96-array format array plates

Visit our website for a complete list of array plates available for use with the Axiom™ 2.0

Assay.

Varies, depending

on array design

Axiom™ Microbiome Array Plate, 96-array format 902904

4Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

(continued)

Item Source

Consumables

Axiom™ GeneTitan™ Consumables Kit

Each kit Includes:

•One 96-layout hybridization tray

•One 96-layout scan tray with cover and protective base

•Five 96-layout stain trays with covers

These trays are required for processing Axiom™ array plates on the GeneTitan™ MC

Instrument.

Note: Consumables are included with the Axiom™ Genome-Wide BOS and Pan-African

Genotyping Bundles.

901606

Training modules

Axiom™ 2.0 Assay Training Module with CEU Arrays

Kit contains:

•2 Axiom™ Genome-Wide CEU 1 Array Plates

•2 full plates of 96 HapMap DNA samples in deep well titer plate[2]

•2 Axiom™ 2.0 Reagent Kits (96 reactions)

•2 Axiom™ GeneTitan™ Consumables Kits

•1 GeneTitan™ ZeroStat AntiStatic Gun

901767

Axiom™ 2.0 Assay Training Module with ASI Arrays

Kit contains:

•2 Axiom™ Genome-Wide ASI 1 Array Plates

•2 full plates of 96 HapMap DNA samples in deep well titer plate[2]

•2 Axiom™ 2.0 Reagent Kits (96 reactions)

•2 Axiom™ GeneTitan™ Consumables Kits

•1 GeneTitan™ ZeroStat AntiStatic Gun

901768

Axiom™ 2.0 Assay Training Module with CEU and BOS Arrays

Kit contains:

•1 Axiom™ Genome-Wide BOS 1 Array Plate

•1 Axiom™ Genome-Wide CEU 1 Array Plate

•1 full plate of 96 HapMap DNA samples in deep well titer plate[2]

•2 Axiom™ 2.0 Reagent Kits (96 reactions)

•2 Axiom™ GeneTitan™ Consumables Kits

•1 GeneTitan™ ZeroStat AntiStatic Gun

901750

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment, software, reagents, and arrays required

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 5

(continued)

Item Source

Axiom™ 2.0 Pan-African Training Module

Kit contains:

•1 Axiom™ Genome-Wide Pan-African Genotyping Bundle (1 set of 96-array plates,

3 Axiom™ 2.0 Reagent Kits, and 3 Axiom™ GeneTitan™ Consumables Kits)

•3 identical 96 HapMap DNA plates[2]

•1 GeneTitan™ ZeroStat AntiStatic Gun

901769

[1] For a complete list of all equipment and supplies required for GeneTitan™ MC Instrument installation and operation, see the

GeneTitan™ Multi-Channel Instrument Site Preparation Guide.

[2] NOTE: Per agreement with Coriell Life Sciences, all residual HapMap DNA samples must be destroyed immediately following

completion of training.

Equipment required for manual target preparation

Instruments required

The following instruments are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow.

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Equipment Source

Two or 3 ovens See “Oven requirements” on page7.

Fume hood (Strongly recommended.) See the Axiom™ 2.0 Assay 96-Array Format

Manual Workﬂow User Guide.

One of the following thermal cyclers:

•Applied Biosystems™ ProFlex™ PCR System

•Applied Biosystems™ GeneAmp™ PCR System 9700

•Applied Biosystems™ 2720 Thermal Cycler

•Bio-Rad™ DNA Engine™ PTC-200 Thermal Cycler/MJ

PTC-200

•Bio-Rad™ DNA Engine Tetrad™ 2 PTC-0240G

See “Thermal cycler recommendations” on page8.

•Thermo Fisher Scientiﬁc

•Bio-Rad Laboratories™

One of the following shakers:

•Thermo Scientiﬁc™ Digital Microplate Shaker

•Thermo Scientiﬁc™ Compact Digital Microplate Shaker

•Jitterbug™ shaker

 

•88882005 or 88882006

•88880023

•Boekel™ Scientiﬁc, 130000

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

6Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

Preampliﬁcation area/ampliﬁcation staging area

Precautions are required when manipulating genomic DNA or setting up ampliﬁcation reactions to avoid

contamination with foreign DNA ampliﬁed in other reactions and procedures. It is recommended that

genomic DNA manipulations and ampliﬁcation reaction set up are performed in a dedicated room or

area separate from the main laboratory.

These areas should have dedicated sets of pipettes and plasticware. If no dedicated area is available,

use of a dedicated bench or a dedicated biosafety hood and dedicated pipettes is suggested. If no

dedicated bench or biosafety hood is available, a set of dedicated pipettes is recommended.

Ideally, the preampliﬁcation and ampliﬁcation staging areas would be separate; however these areas

may be combined due to space and equipment limitations.

Oven requirements

Multiple ovens are required for manual target preparation. The exact number depends upon whether

you are running only a single sample plate and array plate through the workﬂow, or whether you are

trying to run the 3 plate/week manual target preparation protocol.

•If you are running individual plates, you will need 2 ovens for the workﬂow.

•If you are running the 3 plate/week workﬂow, a third oven is highly recommended.

See chapter Processing Three Axiom™ Array Plates per Week with Manual Target Preparation in the

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow User Guide for more information.

Table1Suggested settings for ovens when performing the 3 plate/week manual target preparation

protocol.

Day of workﬂow Oven 1 Oven 2 Oven 3

Day 1 37°C N/A N/A

Day 2 37°C 65°C 37°C

Day 3 48°C 65°C 37°C

Day 4 48°C 65°C 37°C

Day 5 N/A N/A N/A

Two to 3 ovens are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow. Use any

combination of the following ovens.

Table2Oven recommendations.

Equipment Source

Thermo Scientiﬁc™ Heratherm™ Advanced Protocol Microbiological Incubator,

capacity 66 L

•120V, 60 Hz

•230V, 50/60 Hz



 

•51028066

•51028133

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 7

Table 2 Oven recommendations.(continued)

Equipment Source

BINDER™ ED 56 Drying and Heating Chamber

•ED056UL-120V Voltage: 120 V 1~60 Hz

•ED056-230V Voltage: 230 V 1~50/60 Hz

 

•BINDER™, 9010-0334

•BINDER™, 9010-0333

BINDER™ BD 56 Standard-Incubator with natural convection

•BD056UL-120V Voltage: 120 V 1~60 Hz

•BD056-230V Voltage: 230 V 1~50/60 Hz

 

•BINDER™, 9010-0324

•BINDER™, 9010-0323

GeneChip™ Hybridization Oven 645 00-0331

Spectrophotometers

We recommend that you use one of the following spectrophotometers, or the equivalent.

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Item Source

Thermo Scientiﬁc™ Multiskan™ Sky Microplate Spectrophotometer 51119500

DTX 880 Multimode Detector with genomic ﬁlter slide Beckman Coulter™

987921 Detector

A30184 Filter slide

Thermal cycler recommendations

We have veriﬁed the performance of this assay using the following thermal cyclers:

•Applied Biosystems™ ProFlex™ 96‑well PCR System

•Applied Biosystems™ ProFlex™ 2×96‑well PCR System

•Applied Biosystems™ GeneAmp™ PCR System 9700 (with gold-plated or silver block)

•Applied Biosystems™ 2720 Thermal Cycler

•Bio-Rad™ DNA Engine™ PTC-200 Thermal Cycler/MJ PTC-200

•Bio-Rad™ DNA Engine Tetrad™ 2 PTC-0240G

The performance of this assay has not been veriﬁed with other thermal cyclers. Use of other thermal

cyclers may result in assay failure and may violate the Axiom™ array and reagent replacement policy.

The thermal cycler needs to be programmed with the Axiom 2.0 Denature protocol:

95°C

48°C 48°C

10 min

3 min Infinity

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

8Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

Use the heated lid option when setting up or running the protocol.

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 9

Thermal cycler consumables

The following table provides details about the consumables to be used with each thermal cycler.

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Thermal cycler model PCR plate type Seal

Applied Biosystems™ ProFlex™

PCR System

Bio-Rad™Hard-Shell™ 96-Well PCR Plate,

high proﬁle, semi skirted, HSS9601 or

HSS9641.

MicroAmp™ Clear Adhesive

Film (Cat. No. 4306311)

Applied Biosystems™

GeneAmp™ PCR System 9700

(with gold-plated or silver

block)

Bio-Rad™Hard-Shell™ 96-Well PCR Plate,

high proﬁle, semi skirted, HSS9601 or

HSS9641.

MicroAmp™ Clear Adhesive

Film (Cat. No. 4306311)

Applied Biosystems™ 2720

Thermal Cycler

Bio-Rad™Hard-Shell™ 96-Well PCR Plate,

high proﬁle, semi skirted, HSS9601 or

HSS9641.

MicroAmp™ Clear Adhesive

Film (Cat. No. 4306311)

Bio-Rad™ DNA Engine™

PTC-200 Thermal Cycler

Bio-Rad™ Hard-Shell™ 96-Well PCR Plate,

low proﬁle, full skirted, HSP9631 (blue) or

HSP9601 (white).

MicroAmp™ Clear Adhesive

Film (Cat. No. 4306311)

Bio-Rad™ DNA Engine Tetrad™

Bio-Rad™ Hard-Shell™ 96-Well PCR Plate,

low proﬁle, full skirted, HSP9631 (blue) or

HSP9601 (white).

MicroAmp™ Clear Adhesive

Film (Cat. No. 4306311)

Note: Bio-Rad™ Microseal™ 'B' PCR Plate Sealing Film (Bio-Rad™, MSB1001) may be used in place of

MicroAmp™ Clear Adhesive Film for the Bio-Rad™ and Applied Biosystems™ thermal cyclers.

For Axiom™ Microbiome applications, use only the MicroAmp™ Clear Adhesive Film. The Microseal™ 'B'

PCR Plate Sealing Film has not been validated for use with Axiom™ Microbiome applications.

Plate centrifuge

One plate centrifuge is required for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow. We

recommend the plate centrifuges listed in the following table. When centrifuging and drying pellets, the

centrifuge must be able to centrifuge plates at:

•Rcf: 3,200x g (4,000rpm for the Eppendorf™ Centrifuge 5810 R) with an appropriate rotor/bucket

combination

•Temperature: 4°C

In addition, the bottom of the rotor buckets should be soft rubber to ensure that the deepwell plates do

not crack. Do not use buckets where the plates sit directly on a metal or hard plastic bottom.

For the Eppendorf™ Centrifuge 5810 R, do not use the A-4-62 rotor with a WO-15 plate carrier (hard

bottom).

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

10 Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

Table3Plate centrifuges recommended for the Axiom™ 2.0 Assay 96-Array Format Manual

Workﬂow.

Item Source

Sorvall™ Legend™ XTR Centrifuge

(refrigerated), with:

•TX-750 4 + 750mL Swinging Bucket Rotor

•Carrier for microplates (including plate

trays and neoprene pads)

75004521 (60 Hz, 120 V, ﬂoor model)

75004523 (50–60 Hz, 230 V, USA and Canada, ﬂoor model)

75004520 (50 Hz, 230 V, ﬂoor model)

75003017 (bucket)

One of either:

•75003795 (set of 2 carriers)

•75003617 (set of 4 carriers)

Allegra™ 25R Benchtop Centrifuge,

Refrigerated

Beckman Coulter™

369434 (60Hz, 280V)

369435 (50/60Hz, 200V)

369436 (50Hz, 230V)

368954 S5700 Swinging-Bucket Rotor

Centrifuge 5810 R (refrigerated)

A-4-81 MTP/Flex swinging bucket rotor with 4

microtest-plate buckets

Eppendorf™

022625501 (60Hz, 120V)

022625101 (50 Hz, 120 V, 20 AMP version)

022625551 (50Hz, 230V)

022638807

Relative centrifugal force (rcf) can be calculated as follows:

rcf = (1.118 × 10-5) R S2

where

•R is the radius of the rotor in centimeters

•S is the speed of the centrifuge in revolutions per minute

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 11

Sample plates

The following sample plates are required for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow.

Unless otherwise indicated, all materials are available through thermoﬁsher.com.

Equipment Source

Abgene™ 96 Well 2.2 mL Polypropylene Deepwell Storage Plate Mark II,

2.2 mL. (For use with Axiom™ Genotyping Assay.)

AB0932

Eppendorf™ 96 Deepwell Plate, 2,000 µL (For use with Axiom™

Microbiome Assay.)

Note: This is the only plate supported for Axiom™ microbiome use.

Using a dierent plate type may result in assay failure.

Eppendorf™, 951033481

Bio-Rad™ Hard-Shell™ 96-Well PCR Plate, low proﬁle, full skirted Bio-Rad Laboratories™, HSP9631

(blue) or HSP9601 (white)

OD Plate, option 1:

Corning™ UV-Transparent Microplate

Axiom™ 96-format Consumables

Kit for QC, 902909.

Also available from Fisher

Scientiﬁc™, 07-200-623

OD Plate, option 2:

Greiner Bio-One™ UV-Star™ 96-Well UV Spectroscopy Microplate

Fisher Scientiﬁc™, 07-000-407

Bio-Rad™Hard-Shell™ 96-Well PCR Plate, high proﬁle, semi skirted

Required only if using Applied Biosystems™ 9700 or 2720 or ProFlex™

PCR System thermal cyclers.

Bio-Rad Laboratories™, HSS9601

(clear) or HSS9641 (green)

MicroAmp™ EnduraPlate™ Optical 96-Well Clear Reaction Plates with

Barcode

4483354 or 4483352

Pipettes and tips

Pipettes and tips recommended for manual target preparation are listed in the following table.

Equipment Rainin™ part number

Pipet-Lite™, magnetic-assist, single-channel P20 L-20

Pipet-Lite™, magnetic-assist, single-channel P200 L-200

Pipet-Lite™, magnetic-assist, single-channel P1000 L-1000

Pipette, 12-channel P20 L12-20

Pipette, 12-channel P50 (optional) L12-50

Pipette, 12-channel P200 L12-200

Pipette, 12-channel P1200 L12-1200

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

12 Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

(continued)

Equipment Rainin™ part number

Pipette tips GP = reﬁll GP-L10F

Pipette tips GP = reﬁll GP-L200F

Pipette tips GP = reﬁll GP-L1000F

Pipette tips RT = with rack RT-L10F

Pipette tips RT = with rack RT-L200F

Pipette tips RT = with rack RT-L1000F

Other labware required

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that

the material is available from ﬁsherscientiﬁc.com or another major laboratory supplier.

Item Source

Conical tube, 50mL VWR™, 21008-178

Conical tube, 15mL VWR™, 21008-202

Tube Holder, 15 mL and 50mL MLS

Reagent reservoir, 100 mL sterile, multichannel VWR™, 89092-836

25-mL Matrix™ Reagent Reservoir Fisher Scientiﬁc™, 809311

96 well plate metal chamber Diversiﬁed Biotech™,

CHAM-1000

10-mL serological pipettes VWR™, 89130-898 or MLS

5-mL serological pipettes VWR™, 89130-896 or MLS

MicroAmp™ Clear Adhesive Film 4306311

Bio-Rad™ Microseal™ 'B' PCR Plate Sealing Film

Note: For Axiom™ Microbiome applications, use only the MicroAmp™

Clear Adhesive Film. The Microseal™ 'B' PCR Plate Sealing Film has not

been validated for use with Axiom™ Microbiome applications.

Bio-Rad™, MSB1001

Laboratory wipes MLS

1.7-mL microcentrifuge tubes, DNAse and RNAse-free MLS

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 13

Other equipment, reagents, and gels required

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that

the material is available from ﬁsherscientiﬁc.com or another major laboratory supplier.

Table4Other reagents required.

Item Source

2-Propanol, anhydrous, 99.5% (Isopropanol) Sigma-Aldrich™, 278475

TE Buer, 1X Solution pH 8.0, Low EDTA J75793.AP

Table5Equipment, reagents, and gels required to run QC steps.

Item Source

E‑Gel™ Power Snap Plus Electrophoresis Device G9110

Safe Imager™ Viewing Glasses S37103

E‑Gel™ Power Snap Plus Camera G9200

Invitrogen™ E‑Gel™ 48 Agarose Gels, 4% (for Axiom™ QC) G800804

Invitrogen™ E‑Gel™ 48 Agarose Gel, 1% (for gDNA QC) G800801

Invitrogen™ E‑Gel™ 96 High Range DNA Marker (for gDNA QC) 12352019

Invitrogen™ RediLoad™ Loading Buer (for gDNA QC) 750026

Applied Biosystems™25bp DNA Ladder or equivalent (for Axiom™ QC) 931343

Invitrogen™ TrackIt™ Ultra Low Range DNA Ladder 10488023

Invitrogen™ TrackIt™ Cyan/Orange Loading Buer (for Axiom™ QC) 10482028

UltraPure™ DNase/RNase-Free Distilled Water 10977023

Other lab equipment required

Unless otherwise indicated, all materials are available through thermoﬁsher.com. "MLS" indicates that

the material is available from ﬁsherscientiﬁc.com or another major laboratory supplier.

Item Source

Bel-Art™ Cryo-Safe™ Mini Cooler, –15℃[1] Fisher Scientiﬁc™, 03-410-497

Fine-point permanent markers MLS

Freezer, –20°C MLS

Ice bucket, 4—9 liters MLS

Mini Microcentrifuge for 2mL tubes MLS

Electronic pipettor for serological pipettes MLS

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

14 Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide

(continued)

Item Source

Refrigerator, 2—8°C MLS

Vortexer (for plates and microtubes) MLS

[1] Equivalent items from other manufacturers are acceptable.

Equipment and supplies for the Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow

Equipment required for manual target preparation

Axiom™ 2.0 Assay 96-Array Format Manual Workﬂow Site Preparation Guide 15

Documentation and support

Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

Brand: Thermo Fisher Scientific

Model: Axiom 2.0 Assay 96-Array Format

Type: Owner's manual

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Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

Thermo Fisher Scientific Axiom 2.0 Assay 96-Array Format Owner's manual

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